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Relationship between insulin A chain regions and insulin biological activities

World Journal of Gastroenterology 2000年第3期6卷 371-373页

作者： Yang SZ Huang YD Jie XF feng ym Niu JY Chinese Acad Sci Shanghai Inst Biochem Shanghai 200031 Peoples R China

AIM To study the relationship between insulinA chain regions and insulin biological activities,we designed a series of insulin analogues withchanges at A21,A12-18 of C-terminal helicalregion and A8-10 Iocated in the region of A6-A11intra-chain disulphide *** Insulin A-chain analogues wereprepared by stepwise Fmoc solid-phase manualsynthesis and then combined with natural B-chain of porcine insulin to yield correspondinginsulin *** biological activitieswere tested by receptor binding,mouseconvulsion and immunological ***[A21Ala]Ins retains 70.3% receptorbinding capacity and 60% in vivo biologicalactivity.[DesA13-14,A21Ala]Ins and[DesA12-13-14-15,A21Ala]Ins still have definite biologicalactivity,7.9% and 4.0% receptor binding,and6.2% and 3.3% in vivo biological activityrespectively.[A15Asn,A17Pro,A21Ala]Insmaintains 10.4% receptor binding and 10% invivo biological activity.[A8His,A9Arg,A10Pro,A21Ala]Ins,[A8His,A9Lys,A10Pro,A21Ala]Insand [A8His,A9Lys,A10Arg,A21Ala]Ins have51.9%,44.3% and 32.1% receptor bindingrespectively,50%,40% and 30% in vivobiological activity respectively,and 28.8%,29.6% and 15.4% immunological activityrespectively. CONCLUSION A21Asn can be replaced bysimple amino acid *** A chains withgradually damaged structural integrity in A12-18helical region and the demolition of the A12-18helical region by the substitution of Pro and Asnfor A17Glu and A15Gln respectively can combinewith the B chain and the combination productsshow definite biological activity,the helicalstructure of A12-18 is essential for biologicalactivities of insulin.A8-10 is not muchconcerned with biological activities,but is muchmore important antigenically in binding to itsantibodies,these results may help us design anew type of insulin analogue molecule.

关键词： insulin/chemistry biological activities A chain analogues

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间充质干细胞基因转染的相关研究现状

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中国组织工程研究与临床康复 2007年第11期11卷 2118-2121页

作者：冯一梅徐辉解放军第三军医大学军事预防医学院创伤烧伤与复合伤国家重点实验室重庆市400038

目的:对间充质干细胞的基因转染进行介绍,以及对骨形态发生蛋白、转化生长因子、血管内皮细胞生长因子等转染间充质干细胞的相关研究进行综述。资料来源:应用计算机检索Pubmed1999-01/2007-01间充质干细胞基因转染的文章,检索词"mesench... 详细信息

目的:对间充质干细胞的基因转染进行介绍,以及对骨形态发生蛋白、转化生长因子、血管内皮细胞生长因子等转染间充质干细胞的相关研究进行综述。资料来源:应用计算机检索Pubmed1999-01/2007-01间充质干细胞基因转染的文章,检索词"mesenchymal stem cells,gene transfer",文章语言限定为英语,同时检索中文CNKI全文数据库1999-01/2007-01间充质干细胞基因转染的文章,检索词"间充质干细胞,基因转染",文章语言限定为中文。资料选择:对资料进行初审,并查看每篇文章后的引文。纳入间充质干细胞基因转染的相关实验研究及综述文献,排除陈旧、重复文献。资料提炼:共收集到165篇相关文献,入选29篇。资料综合:间充质干细胞具有高可塑性、可移植性、易接受外源基因、可以传送基因、自身可以参与组织修复等特点,被广泛应用于骨形态发生蛋白、转化生长因子、血管内皮细胞生长因子等的转染,成为基因治疗的理想靶细胞。但间充质干细胞转基因技术在基因工程中还面临一些问题,如完善转基因技术,以及使转染后的靶细胞具有更大的生物效应等。结论:随着研究的进一步深入,间充质干细胞介导的细胞和基因治疗将会逐步成熟,有望更好的用于临床治疗。

关键词：间充质干细胞基因转染综述文献

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Toughening of Epoxy Resin with Microspheres

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Journal of Materials Science & Technology 1996年第1期12卷 46-50页

作者： Jin, SJ feng, SB Pang, JQ Hua, XL Li, ym Yu, YH Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

Special microspheres dispersed in epoxy resin prior to curing can toughen brittle resin by formation of a second phase. In this work, rigid particles (HP), hollow particles (PO) and rubber powders (PR) are used to modify epoxy resins for the study on the toughening mechanism. The single-edge notched three-point bending test and T peel test have been used to measure the toughness of epoxy resin. The morphology (particle/matrix interfacial shape) of epoxy resin modified with these three kinds of microspheres has been investigated by transmission electron microscopy (TEM). The results show that these three kinds of modifiers are all effective, and the core-shell rubber particle (PR) is the best toughening modifier among them. It is also found that the toughening effect is dependent on the morphology of the interface between the particles and matrix and the different curing conditions really result in obviously different interface states. The excellent interfacial interaction plays a significant role in toughening epoxy resin.

关键词： Epoxy resins

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恶性肿瘤患者血清与尿液中一氧化氮含量测定

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第四军医大学学报 2001年第4期22卷 F002-F002页

作者：黄克楠冯英明张贺龙姬统理闵婕第四军医大学唐都医院肿瘤科陕西西安710038

0 引言一氧化氮（Nitric oxide，NO）是一种具有活跃生物化学性质的无机小分子. NO对许多肿瘤细胞和微生物有细胞毒性［1］，为探讨NO与肿瘤的关系，我们检测了119例恶性肿瘤患者血清及尿液中的NO.

关键词：肿瘤一氧化氮血清尿液标本采集

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速度滑冰与短道速度滑冰运动员身体功能评定

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中国组织工程研究与临床康复 2007年第39期11卷 7943-7945页

作者：葛艺明冯松冯长坤沈阳农业大学军体部辽宁省沈阳市110030 北京大学软件与微电子学院北京市100871 解放军冰上训练基地辽宁省沈阳市110032

目的:总结速度滑冰与短道速度滑冰运动员各项生理生化指标,开发应用生理生化指标对身体功能进行评定,为科学训练提供参考依据。方法:分析速度滑冰与短道速度滑冰项目特点,并根据其特点制定了各项生理生化指标的参考值。结果:速度滑冰与... 详细信息

目的:总结速度滑冰与短道速度滑冰运动员各项生理生化指标,开发应用生理生化指标对身体功能进行评定,为科学训练提供参考依据。方法:分析速度滑冰与短道速度滑冰项目特点,并根据其特点制定了各项生理生化指标的参考值。结果:速度滑冰与短道速度滑冰均有周期性耐力性项目特点,要求运动员必须具备较强的无氧代谢能力,尤其是抗乳酸能力,可用4mmol/L做无氧阈训练监控指标。血尿素参考值4￣7mmol/L,功能下降或训练量大时增高。血清肌酸激酶强度训练时可高达16.67μkat/L以上。血红蛋白参考值为男120￣160g/L,女110￣150g/L。免疫指标和内分泌指标均应定期监测,为提供身体功能信息有重要意义。结论:可用血乳酸系统监控和评估有氧与无氧耐力水平,运动员身体功能评定是科学化训练重要保证,是科学训练的重要部分。

关键词：速度滑冰短道速度滑冰血乳酸身体机能

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Neuroprotective mechanisms ofε-viniferin in a rotenone-induced cell model of Parkinson's disease:significance of SIRT3-mediated FOXO3 deacetylation

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Neural Regeneration Research 2020年第11期15卷 2143-2153页

作者： Shuo Zhang Yan Ma Juan feng Department of Neurology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Department of Ultrasound Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Trans-(-)-ε-viniferin(ε-viniferin)has antioxidative and anti-inflammatory *** also has neuroprotective effects in Huntington's disease by activating the SIRT3/LKB1/AMPK signaling pathway;however,it remains unknown whetherε-viniferin also has a neuroprotective role in Parkinson's disease.A Parkinson's disease cell model was induced by exposing SH-SY5 Y cells to 3.0μM rotenone for 24 hours,and cells were then treated with 1.0μMε-viniferin for 24 *** withε-viniferin upregulated SIRT3 expression,which promoted FOXO3 deacetylation and nuclear localization.ε-Viniferin also increased ATP production and decreased reactive oxygen species ***,ε-viniferin treatment alleviated rotenone-induced mitochondrial depolarization and reduced cell apoptosis,and restored the expression of mitochondrial homeostasis-related ***,when cells were transfected with SIRT3 or FOXO3 shRNA prior to rotenone andε-viniferin treatment,these changes were *** results from the present study indicate thatε-viniferin enhances SIRT3-mediated FOXO3 deacetylation,reduces oxidative stress,and maintains mitochondrial homeostasis,thus inhibiting rotenone-induced cell apoptosis.ε-Viniferin may therefore be a promising treatment strategy for Parkinson's disease.

关键词： deacetylation FOXO3 mitochondrial homeostasis mitophagy oxidative stress Parkinson's disease SIRT3 ε-viniferin

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Spectral Fittings of Warm Coronal Radiation with High Seed Photon Temperature:Apparent Low-temperature and Flat Soft Excess in AGNs

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Research in Astronomy and Astrophysics 2024年第7期24卷 135-144页

作者： Ze-Yuan Tang Jun-Jie feng Jun-Hui Fan School of Mathematics and Information Science Guangzhou UniversityGuangzhou 510006China Center for Astrophysics Guangzhou UniversityGuangzhou 510006China Astronomy Science and Technology Research Laboratory of Department of Education of Guangdong Province Guangzhou 510006China Key Laboratory for Astronomical Observation and Technology of Guangzhou Guangzhou 510006China

A warm corona has been widely proposed to explain the soft excess(SE)in X-ray above the 2-10 ke V power law extrapolation in Active Galactic Nuclei(AGNs).In actual spectral fittings,the warm coronal seed photon temperature(T_s)is usually assumed to be far away from the soft X-ray,but kT_scan reach close to 0.1 ke V in the standard accretion disk *** this study,we used Monte Carlo simulations to obtain radiation spectra from a slab-like warm corona and fitted the spectra using the spherical-geometry-based routine THCOMP or a thermal *** findings reveal tha high T_scan influence the fitting results.A moderately high kT_s(around 0.03 ke V)can result in an apparent low-temperature and flat SE,while an extremely high kT_s(around 0.07 ke V)can even produce an unobserved blackbody-like *** conclusions indicate that,for spectral fittings of the warm coronal radiation(SE in AGNs),kT_sshould be treated as a free parameter with an upper limit,and an accurate coronal geometry is necessary when kT_s>0.01 ke V.

关键词： galaxies active-accretion-accretion disks-black hole physics

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静脉移植骨髓间充质干细胞对大鼠脊髓损伤后生长相关蛋白43、神经丝蛋白及巢蛋白的调节

引用

中国组织工程研究与临床康复 2007年第42期11卷 8421-8425页

作者：冯大雄康建平宋跃明泸州医学院附属医院骨科四川省泸州市646000 四川大学华西医院骨科四川省成都市610041

目的:已证实骨髓间充质干细胞不仅具有向中胚层间质细胞分化的能力,在一定条件下或微环境中还能够向外胚层和内胚层细胞分化。为此观察静脉移植骨髓间充质干细胞对脊髓损伤后生长相关蛋白43、神经丝蛋白及巢蛋白的影响。方法:实验于2004... 详细信息

目的:已证实骨髓间充质干细胞不仅具有向中胚层间质细胞分化的能力,在一定条件下或微环境中还能够向外胚层和内胚层细胞分化。为此观察静脉移植骨髓间充质干细胞对脊髓损伤后生长相关蛋白43、神经丝蛋白及巢蛋白的影响。方法:实验于2004-12在四川大学华西医院组织工程实验室完成。①实验动物:雌性SD大鼠66只,随机数字表法分为3组:细胞移植组、注射液对照组、模型对照组,22只/组。另取6周龄SD大鼠10只用于骨髓间充质干细胞的分离培养。②实验方法:应用密度梯度离心、贴壁培养和酶消化控制相结合,分离纯化鼠骨髓间充质干细胞。传至第6代在移植前1d予以BrdU标记,制成浓度约为2×109L-1细胞悬液。各组大鼠均建立脊髓损伤模型,造模后1周作尾静脉穿刺,细胞移植组注入骨髓间充质干细胞悬液1mL,注射液对照组注入等量的无血清低糖培养基,模型对照组未作处理。③实验评估:分别于细胞移植后2,3,6周取材,免疫组织化学检测骨髓间充质干细胞在损伤段脊髓的存活情况,观察损伤段脊髓内生长相关蛋白43、神经丝蛋白及巢蛋白的表达。结果:①骨髓间充质干细胞存活及计数:移植后2,3,6周BrdU阳性的骨髓间充质干细胞主要分布于损伤段及邻近节段脊髓内,计数分别为(9830±874)个,(8780±720)个,(5700±420)个,各占移植细胞总量的4.9%,4.4%,2.9%。②生长相关蛋白43及神经丝蛋白的表达:细胞移植后2,3,6周,细胞移植组生长相关蛋白43及神经丝蛋白的表达面积均明显高于其余两组(P<0.05)。③巢蛋白的表达:细胞移植后2,3周,细胞移植组的巢蛋白阳性细胞数均明显高于其余两组(P<0.05)。至第6周细胞移植组巢蛋白阳性细胞数明显降低(P<0.05),其余两组不表达。结论:脊髓损伤后静脉移植的骨髓间充质干细胞能向脊髓损伤灶内迁徙、存活,上调生长相关蛋白43、经丝蛋白及巢蛋白的表达。

关键词：骨髓间充质干细胞脊髓损伤细胞移植

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Comparison of intracytoplasmic sperm injection(ICSI)outcomes in infertile men with spermatogenic impairment of differing severity

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Asian Journal of Andrology 2022年第3期24卷 299-304页

作者： Ping Ping Zhong Zheng Yi Ma Sha-Sha Zou Xiang-feng Chen Center for Reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200135China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200135China Shanghai Human Sperm Bank Shanghai 200135China

The extent of spermatogenic impairment on intracytoplasmic sperm injection(ICSI)outcomes and the risk of major birth defects have been little *** this study,we evaluated the relationship between various spermatogenic conditions,sperm origin on ICSI outcomes,and major birth defects.A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji Hospital(Shanghai,China)were classified into six groups:nonobstructive azoospermia(NOA;n=84),extremely severe oligozoospermia(esOZ;n=163),severe oligozoospermia(sOZ,n=174),mild oligozoospermia(mOZ;n=148),obstructive azoospermia(OAZ;n=155),and normozoospermia(NZ;n=210).Rates of fertilization,embryo cleavage,high-quality embryos,implantation,biochemical and clinical pregnancies,abortion,delivery,newborns,as well as major birth malformations,and other newborn outcomes were analyzed and compared among *** NOA group showed a statistically lower fertilization rate(68.2%vs esOZ 77.3%,sOZ 78.0%,mOZ 73.8%,OAZ 76.6%,and NZ 79.3%,all P<0.05),but a significantly higher implantation rate(37.8%)than the groups esOZ(30.1%),sOZ(30.4%),mOZ(32.6%),and OAZ(31.0%)(all P<0.05),which was similar to that of Group NZ(38.4%).However,there were no statistically significant differences in rates of embryo cleavage,high-quality embryos,biochemical and clinical pregnancies,abortions,deliveries,major birth malformations,and other newborn outcomes in the six *** results showed that NOA only negatively affects some embryological outcomes such as fertilization *** was no evidence of differences in other embryological and clinical outcomes with respect to sperm source or spermatogenic *** failure and sperm origins do not impinge on the clinical outcomes in ICSI treatment.

关键词： intracytoplasmic sperm injection nonobstructive azoospermia normozoospermia obstructive azoospermia oligozoospermia

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Clinical efficacy of the partial rectus muscle transportation procedure for paralytic strabismus

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 978-984页

作者： Yan-Qiong Tu Xiao-Ying Wu Jie-Yue Wang feng-Yun Li Eye Center of Xiangya Hospital Central South UniversityChangsha 410008Hunan ProvinceChina Hunan Key Laboratory of Ophthalmology Changsha 410008Hunan ProvinceChina

AIM:To analyze the clinical efficacy of the partial rectus muscle transportation(PRT)procedure for paralytic strabismus due to single rectus muscle ***:In total,28 patients(31 eyes)who underwent the PRT procedure for paralytic strabismus due to single rectus muscle palsy were retrospectively *** following data were collected pre-and postoperatively:angle of deviation in the primary position,presence of diplopia in the primary position,presence of compensatory head posture,and motility of the affected *** follow-up period was 6 ***:Based on the preoperative and intraoperative findings,different operations were performed:2 eyes were treated with PRT,26 eyes were treated with PRT combined with the recession of the antagonist muscle(Am)of the paralytic rectus muscle,and 3 eyes were treated with PRT along with the recession of the Am and the yoke muscle(ym).On the first day after the operation,24 patients were found to be orthophoric in the primary position,without diplopia or abnormal head ***,2 patients with monocular lateral rectus muscle palsy had mild overcorrection to 5 prism diopters(PD)and 8 PD,respectively,whereas 2 patients with binocular lateral rectus muscle palsy had mild undercorrection to 8 PD and 10 PD,*** the 6-month follow-up period,the mean deviation was rectified from 96.79±41.21 PD to 0.64±2.38 PD(t=12.48,P<0.001),whereas the deviations in the 2 patients with mild overcorrection were corrected to *** mean preoperative limitation of motility improved from-4.55±0.51 to-2.65±0.61(t=-15.13,P<0.001)after 6 mo ***:PRT is an effective treatment for complete paralytic strabismus due to single rectus muscle palsy,and can achieve stable clinical efficacy.

关键词： ophthalmoplegia strabismus rectus transposed procedure

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