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Breviscapine alleviates hepatic injury and inhibits PKC-mRNA and its protein expression in brain-dead BA-Ma mini pigs

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Hepatobiliary & Pancreatic Diseases International 2007年第6期6卷 604-609页

作者： Zhang, Shui-Jun Song, Yan Zhai, Wen-Long Shi, Ji-Hua feng, liu-shun Zhao, Yong-Fu Chen, Shi Zhengzhou Univ Affiliated Hosp 1 Dept Surg Zhengzhou 450052 Peoples R China Huazhong Univ Sci & Technol Tongi Med Coll Tongji Hosp Inst Transplantat Wuhan 430030 Peoples R China

BACKGROUND: Brain-dead donors are the main sources for organ transplantation, but many studies show that brain-death affects the organ's function after transplantation. This study was undertaken to investigate liver injury after brain-death in BA-Ma mini pigs and the protective effects of breviscapine on hepatic function and on PKC-alpha mRNA and its protein expression. METHODS: Fifteen BA-Ma mini pigs were equally divided into 3 groups at random: brain-dead (group B), breviscapine pretreated (group P), and control (group Q. The brain-dead model was established by increasing intracranial pressure in a modified, slow and intermittent way. At 3, 6, 12, 18 and 24 hours after the initial brain-death, the levels of serum AST, ALT, TNF-alpha, IL-1 beta, and IL-6 were determined. The changes in hepatic tissues were assessed, and the expression of PKC-alpha and PKC-alpha mRNA was detected by immunohistochemistry and RTPCR, respectively. RESULTS: The levels of AST and ALT in groups B and P began to increase 12 hours after brain-death, while the values in group P were lower than those in group B (P<0.05). The levels of IL-1 beta, IL-6, and TNF-alpha in groups B and P at 3, 6, 12 and 18 hours were lower than those in group B (P<0.05). At 6, 12 and 24 hours, the expressions of PKC-a mRNA and PKC-a protein in group P were lower than those in group B (P<0.05). The degree of injury to hepatic cells in group P was milder than that in group B. CONCLUSIONS: Breviscapine inhibits the degree of PKC-alpha mRNA transcription and its protein translation, decreases the release of inflammatory factors, and thus alleviates hepatic injury during brain-death.

关键词： breviscapine BA-Ma mini pigs brain-death protein kinase C

Synthesis and in vitro Antimycobacterial Activity of Moxifloxacin Methylene and Ethylene Isatin Derivatives

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Chemical Research in Chinese Universities 2012年第1期28卷 61-66页

作者： feng Lian-shun, liu Ming-liang, WANG Shuo, CHAI Yun, LI Su-jie and GUO Hui-yuan Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P. R. China Chinese Acad Med Sci Inst Med Biotechnol Beijing 100050 Peoples R China

A series of novel moxifloxacin methylene and ethylene isatin derivatives with remarkable improvement in lipophilicity, compared to the parent moxifloxacin, was designed, synthesized and characterized by 1H NMR, MS and HRMS. These derivatives were initially evaluated for their in vitro antimycobacterial activity against M. smegmatis CMCC 93202. Compounds 3a―3f, 5a, 5f and 5j were chosen for the further evaluation of their in vitro activity against Mycobacterium tuberculosis（MTB） H37Rv ATCC 27294 and MDR-MTB 09710. All the target com pounds[minimum inhibitory concentration（MIC）： 0.39―〉16 μg/mL] were far more active than rifampin（MIC： 2.0―〉256 μg/mL）, but less active than moxifloxacin（MIC： 0.1―1.0 μg/mL） against the three tested strains. The most active compounds 3a and 3c were found to be 2―64 fold more potent than isoniazid and rifampin against M. smegmatis CMCC 93202, 2 fold more potent than rifampin against MTB H37Rv ATCC 27294, and 16―〉64 fold more potent than ethambutol, isoniazid and rifampin against MDR-MTB 09710.

关键词： Moxifloxacin derivative Antimycobacterial activity Isatin

Smart Gas Sensors:Recent Developments and Future Prospective

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Nano-Micro Letters 2025年第3期17卷 55-86页

作者： Boyang Zong Shufang Wu Yuehong Yang Qiuju Li Tian Tao shun Mao College of Environmental Science and Engineering Biomedical Multidisciplinary Innovation Research InstituteShanghai East HospitalState Key Laboratory of Pollution Control and Resource ReuseTongji University1239 Siping RoadShanghai 200092People’s Republic of China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092People’s Republic of China Microbiome Medicine Center Department of Laboratory MedicineZhujiang HospitalSouthern Medical UniversityGuangzhou 510280People’s Republic of China

Gas sensor is an indispensable part of modern society withwide applications in environmental monitoring,healthcare,food industry,public safety,*** the development of sensor technology,wireless communication,smart monitoring terminal,cloud storage/computing technology,and artificial intelligence,smart gas sensors represent the future of gassensing due to their merits of real-time multifunctional monitoring,earlywarning function,and intelligent and automated *** electronicand optoelectronic gas sensors have been developed for high-performancesmart gas *** the development of smart terminals and the maturityof integrated technology,flexible and wearable gas sensors play an increasingrole in gas *** review highlights recent advances of smart gassensors in diverse *** structural components and fundamentalprinciples of electronic and optoelectronic gas sensors are described,andflexible and wearable gas sensor devices are ***,sensorarray with artificial intelligence algorithms and smart gas sensors in“Internet of Things”paradigm are ***,the challengesand perspectives of smart gas sensors are discussed regarding the future need of gas sensors for smart city and healthy living.

关键词： Smart gas sensor Electronic sensor Optoelectronic sensor Flexible and wearable sensor Artificial intelligence

基于地理信息系统的土地利用变化制图方法研究——以黄河小浪底水库库周为例

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水土保持学报 2003年第2期17卷 110-113页

作者：李道峰柳长顺刘昌明郝芳华北京师范大学环境科学研究所水环境模拟国家重点实验室北京100875

详细介绍了土地利用变化图绘制的一般过程,认为图例的设计和不同时期土地利用现状图的代数叠加运算是整个编制过程的关键,制作过程中应本着简单实用、形象答意和美观大方的原则进行。实例选取黄河小浪底水利枢纽库周1993年和2000年TM遥... 详细信息

详细介绍了土地利用变化图绘制的一般过程,认为图例的设计和不同时期土地利用现状图的代数叠加运算是整个编制过程的关键,制作过程中应本着简单实用、形象答意和美观大方的原则进行。实例选取黄河小浪底水利枢纽库周1993年和2000年TM遥感影像进行土地利用变化分析,编制出建库前后土地利用变化图,计算出土地覆被变化转移矩阵,并对土地利用变化结果进行分析。

关键词：地理信息系统土地利用变化制图方法代数叠加运算黄河小浪底水库库周转移矩阵变化图例

Ni-catalyzed cross-electrophile alkyl-alkyl coupling reactions

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Science China Chemistry 2025年

作者： Li-Xu You Lan Tian Chun-Ling Guo shun-Xi Li Yu-Cheng liu Yu-Long Li Wei Shu College of Chemistry and Environmental Engineering Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan Sichuan University of Science and Engineering Guangming Advanced Research Institute Shenzhen Grubbs Institute Department of Chemistry and Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and Technology

Introducing saturated carbon-carbon bonds into organic molecules significantly increases the three-dimensional configuration,thus altering the physical and chemical properties as well as the functions of molecules. Transition-metal-catalyzed carboncarbon cross-couplings represent one of the most straightforward, efficient and modular alternatives to build saturation in a single step. However, the efficient construction of alkyl-alkyl bonds remains a formidable challenge due to the precise manipulation of multiple alkyl metallic intermediates as well as associated competitive side reactions and selectivity control. Over the past years, Ni-catalyzed cross-coupling under reductive conditions has gained more and more attention for alkyl-alkyl crosscoupling partially attributing to the low-valent state of metal centers under reducing conditions, which suppresses the competitive β-hydride elimination process, thus facilitating the formation of alkyl-alkyl bonds. In this review, the initiative and progress of Ni-catalyzed cross-electrophile alkyl-alkyl cross-coupling, including the efforts in racemic and enantioselective formation of alkyl-alkyl bonds, have been summarized and discussed. Moreover, the challenges and further opportunities in this area have also been discussed.

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脾静脉-下腔静脉人工血管架桥术加贲fIN围血管缝扎术治疗门脉高压26例疗效观察

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中国实用医刊 2013年第11期40卷 57-59页

作者：冯留顺郭俊强李捷郑州大学第一附属医院肝胆外科 450052

目的探讨脾静脉．下腔静脉人工血管架桥术加贲门周围血管缝扎术治疗门脉高压的疗效。方法对郑州大学第一附属医院自2006年12月至2012年1月26例门脉高压症患者行脾切除加睥静脉一下腔静脉人工血管架桥术加贲门周围血管缝扎术治疗的临床... 详细信息

目的探讨脾静脉．下腔静脉人工血管架桥术加贲门周围血管缝扎术治疗门脉高压的疗效。方法对郑州大学第一附属医院自2006年12月至2012年1月26例门脉高压症患者行脾切除加睥静脉一下腔静脉人工血管架桥术加贲门周围血管缝扎术治疗的临床资料进行回顾性分析，对手术并发症、病死率，近远期疗效及再出血率进行追踪统计。结果本研究无死亡病例，无肝功能衰竭、肝肾综合征及肝肺综合征，其他并发症：轻度肝性脑病1例，人工血管血栓形成3例，切口感染1例，肺部感染1例，切口感染、肺部感染、肝性脑病均通过非手术治疗后痊愈，人工血管血栓形成2例通过溶栓、抗凝治疗后症状消失。术后再出血2例，1例为胃黏膜病变，药物治疗痊愈，1例为人工血管血栓形成导致食管静脉曲张破裂出血，经内镜套扎止血后出血停止。术后26例脾亢完全缓解，18例腹水患者中17例患者的腹水明显消退，术后1个月21例患者肝功能较术前改善，5例无明显变化。结论脾静脉一下腔静脉人工血管架桥术加贲门周围血管缝扎术能很好地治疗门脉高压所致的脾亢、腹水及消化道出血，改善肝功能，其在降低术后再出血率、控制腹水方面优于脾切断流术。

关键词：缝扎术门脉高压脾-腔分流消化道出血

MMP-9及TIMP-1在重症急性胰腺炎大鼠肺组织中的表达及生长抑素的影响

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实用医学杂志 2007年第12期23卷 1806-1808页

作者：彭启平冯留顺李珂浙江省余姚市人民医院普外科 315400 郑州大学第一附属医院普外科 450052 中南大学湘雅三医院移植中心长沙市410013

目的:观察基质金属蛋白酶-9(MMP-9)及其组织抑制剂TIMP-1在重症急性胰腺炎(SAP)大鼠肺组织中的动态表达,并探讨其在继发肺损伤中的作用,观察生长抑素对SAP肺损伤的治疗作用。方法:采用胰被膜下多点均匀注射5%牛磺胆酸钠建立大鼠SAP模型... 详细信息

目的:观察基质金属蛋白酶-9(MMP-9)及其组织抑制剂TIMP-1在重症急性胰腺炎(SAP)大鼠肺组织中的动态表达,并探讨其在继发肺损伤中的作用,观察生长抑素对SAP肺损伤的治疗作用。方法:采用胰被膜下多点均匀注射5%牛磺胆酸钠建立大鼠SAP模型。观察炎性介质(淀粉酶、TNF-α、IL-6)、肺体指数、肺组织髓过氧化物酶水平的变化,胰腺、肺脏病理变化及MMP-9、TIMP-1在肺组织中的表达。结果:SAP可以诱导炎性介质释放增多,同时MMP-9及TIMP-1表达上调,MMP-9/TIMP-1比例失衡,并与肺损伤的严重程度呈正相关。生长抑素治疗后MMP-9表达下调,MMP-9/TIMP-1比例部分恢复,肺损伤程度减轻。结论:细胞因子介导的肺组织MMP-9/TIMP-1表达比例失衡,可能是SAP继发肺损伤机制的重要环节。

关键词：胰腺炎,急性坏死性明胶酶B 大鼠 TIMP-1 生长抑素

脑死亡对巴马小型猪心脏结构与功能的影响及其机制的实验研究

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中国病理生理杂志 2007年第8期23卷 1480-1483页

作者：张水军朱盛兴李捷马秀现冯留顺范正军郑州大学第一附属医院外科河南郑州450052

目的:探讨脑死亡状态对巴马小型猪心脏结构与功能的影响及蛋白激酶C(PKC)在其发生机制中的作用。方法:巴马小型猪10只,随机分为脑死亡组与对照组,每组5只。应用改进的缓慢间断颅内加压法建立脑死亡模型,对照组仅开颅麻醉维持。于脑死亡... 详细信息

目的:探讨脑死亡状态对巴马小型猪心脏结构与功能的影响及蛋白激酶C(PKC)在其发生机制中的作用。方法:巴马小型猪10只,随机分为脑死亡组与对照组,每组5只。应用改进的缓慢间断颅内加压法建立脑死亡模型,对照组仅开颅麻醉维持。于脑死亡后6、12和24 h检测血清中肌钙蛋白T(cTnT)、IL-1β、IL-6及TNF-α水平,24 h开胸取心肌组织,HE染色观察心脏组织结构变化,电镜观察心脏超微结构变化,免疫组化染色观察PKC-α蛋白表达,RT-PCR法检测PKC-αmRNA表达。结果:(1)心肌组织学变化:脑死亡后24 h可见心肌结构改变,光镜下见心内膜下片状出血,心肌溶解,断裂;电镜下见线粒体水肿,肌纤维模糊融合,嵴部分消失,膜部分融合。(2)血清cTnT变化:脑死亡组血清cTnT自脑死亡后6 h开始升高,并随时间延长而逐渐升高;各时点两组相比脑死亡组明显高于对照组(P<0.05)。(3)炎症介质变化:脑死亡组IL-1β、IL-6、TNF-α水平自6 h开始升高,并随时间延长而逐渐升高;各时点两组相比脑死亡组明显高于对照组(P<0.05)。(4)心肌细胞中PKC-αmRNA及其蛋白水平的变化:脑死亡组PKC-αmRNA及其蛋白表达水平均明显高于对照组(P<0.05)。结论:脑死亡导致巴马小型猪心脏结构与功能的损伤,炎症介质水平升高;脑死亡时心脏PKC-α表达水平升高。PKC-α的活化可能参与了脑死亡状态下心脏的损伤过程。

关键词：巴马小型猪脑死亡蛋白激酶C 心脏损伤

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MMP-9在胰腺癌中的表达及意义

大家健康(学术版) 2013年第17期 46-47页

作者：李旭辉易晓雷冯留顺长沙市八医院普通外科郑州大学第一附属医院普通外科

目的:探讨MMP-9在胰腺癌发生、发展及转移过程中的意义。方法:采用免疫组织化学S-P法,检测MMP-9在53例胰腺癌组织(胰腺癌组)及10例正常胰腺组织(对照组)中的表达,并与临床病理学资料进行分析比较。结果:胰腺癌组织中MMP-9的阳性表达明... 详细信息

目的:探讨MMP-9在胰腺癌发生、发展及转移过程中的意义。方法:采用免疫组织化学S-P法,检测MMP-9在53例胰腺癌组织(胰腺癌组)及10例正常胰腺组织(对照组)中的表达,并与临床病理学资料进行分析比较。结果:胰腺癌组织中MMP-9的阳性表达明显高于正常胰腺组织(P<0.05)。MMP-9的表达与胰腺癌的临床分期及淋巴结有无转移有关(P<0.05)。结论:胰腺癌组织中MMP-9的表达与淋巴结转移及临床分期相关。

关键词：胰腺癌 MMP-9