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Tim-3 mRNA expression in peripheral blood lymphocytes from asthmatic patients

Frontiers of Medicine 2009年第2期3卷 187-190页

作者： Xiaoxia LU Weikun HU Shengdao XIONG Guopeng XU fen lan Department of Respiratory Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Ophthalmology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

The study aimed to detect the expression of the Th1-speciﬁc cell surface protein T cell Ig and mucin domain-containing molecule-3(Tim-3)mRNA peripheral blood lymphocytes isolated from asthmaticin patients and to examine the correlation among Tim-3 mRNA,interleukin-4(IL-4),interferon-γ(IFN-γ)level,and FEV1/FVC(force expiratory volume in one second/forced vital capacity)to explore the role of Tim-3 in the development and progression of asthmatic inﬂ***-3 mRNA expression was detected by reverse transcription polymerase chain reaction(RT-PCR).The IL-4 and IFN-γlevels were determined by using enzyme-linked immunosorbent assay(ELISA).The correlation among Tim-3 mRNA,IL-4,IFN-γlevel,and pulmonary ventilatory capacity was *** expres-sion of Tim-3 mRNA in patients with acute asthma exacerbation was 0.39�0.06,signiﬁcantly higher than that in patients at the remission stage and controls(0.18�0.05 and 0.07�0.03,P<0.05).The level of IL-4 in patients with acute asthma exacerbation was 68.42�10.54,signiﬁcantly higher than that in the patients at the remission stage and controls(41.83�9.37 and 32.75�8.16,P<0.05).The level of IL-4 in patients in remission was signiﬁcantly higher than that in controls(P<0.05).The level of IFN-γin patients with acute asthma exacerbation was 65.74�7.85,signiﬁcantly lower than that in patients in remission and the control group(120.84�11.62 and 139.65�13.47,P<0.05).The level of IFN-γin patients in asthma remission was signiﬁcantly lower than that in controls(P<0.05).Tim-3 mRNA expression was positively correlated with the level of IL-4(r=0.68,P<0.05)and negatively with the level of IFN-γand pulmonary ventilatory capacity(r=–0.85,r=–0.76,both P<0.01).The increased expression of Tim-3 mRNA in peripheral blood lymphocytes might be involved in the development and progression of asthmatic inﬂammation.

关键词： asthma airway inﬂammation peripheral blood lymphocyte Tim-3

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Expression of Syk in non-small cell lung cancer and its relationship with clinicopathological parameters

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Frontiers of Medicine 2009年第1期3卷 41-44页

作者： fen lan Shengdao XIONG Weining XIONG Guopeng XU Xiaoxia LU Department of Respiratory Medicine Key Laboratory of Pulmonary Diseases of Ministry of Health of ChinaTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430300China

This study aims to research the expression of spleen tyrosine kinase(Syk)in non-small cell lung cancer(NSCLC)and the relationship between Syk and clinico-pathologic factors and *** was applied to detect the expression of Syk and p53 protein in 39 cases of NSCLC(23 cases of lung squamous cell can-cer,16 cases of lung adenocarcinoma)and tumor-sur-rounding normal lung *** positive rate of Syk was 46.15%(18/39)and 100%(39/39)in NSCLC and tumor-surrounding normal lung tissues,*** expres-sion level of Syk in NSCLC was signiﬁcantly lower than that in tumor-surrounding normal lung tissues(P=0.000).The Syk expression was positively correlated with the p53 expression in NSCLC specimens(P=0.025).There was no signiﬁcant association between Syk expression and lymph node metastasis,differentiation degree,tumor size and tumor node metastasis(TNM).The present study demonstrated that Syk was aberrantly expressed in the NSCLC and might have a signiﬁcant impact on tumor growth and progression.

关键词： Syk kinase carcinoma,non-small-cell lung tumor suppressor protein p53

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A rabbit model of pediatric nonalcoholic steatohepatitis: The role of adiponectin

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World Journal of Gastroenterology 2009年第8期15卷 912-918页

作者： Jun-fen Fu Yan-lan Fang Li Liang Chun-Lin Wang Fang Hong Guan-Ping Dong Department of Endocrinology The Children's Hospital of Zhejiang University School of Medicine Hangzhou 310003 Zhejiang Province China

AIM： To create a rabbit model of pediatric nonalcoholic steatohepatitis （NASH） and to evaluate the role of adiponectin in the process. METHODS： Thirty-two specific pathogen-free male New Zealand rabbits were divided randomly into three groups：（1） the normal control group （n = 10） was fed with standard diet for 12 wk; （2） the model group A （n = 11）; and （3） model group B （n = 11） were fed with a highfat diet （standard diet ＋ 10% lard ＋ 2% cholesterol） for 8 and 12 wk, respectively. Hepatic histological changes were observed and biochemical parameters as well as serum levels of adiponectin, interleukin （IL）-6, IL-10 and tumor necrosis factor （TNF）-α were measured. RESULTS： Typical histological hepatic lesions of NASH were observed in both model groups described as liver steatosis, liver inflammatory infiltration, cytologic ballooning, perisinusoidal fibrosis and overall fibrosis. Compared with the normal control group, there were significant increases in model groups A and B in weight gain （1097.2 ± 72.3, 1360.5± 107.6 vs 928.0 ±58.1, P 〈 0.05, P 〈 0.01）, liver weight （93.81±6.64, 104.6±4.42 vs 54.4±1.71, P 〈 0.01）, Lg （ALT）（1.9±0.29, 1.84± 0.28 vs 1.60±0.17, P 〈 0.01）, and Lg （TG）（1.03 ±0.24, 1.16 ±0.33 vs 0.00 ±0.16, P 〈 0.01）. Weight gain was much more in model group B than in model group A （1360.5± 107.6 vs 1097.2 ±72.3, P 〈 0.05）. But, there was no significant difference between the two groups concerning the other indexes. Pro-inflammatory cytokines （IL-6 and TNF-α） increased in model group B compared with that of control and model group A （IL-6：1.86±0.21 vs 1.41 ±0.33, 1.38± 0.42, P 〈 0.01; TNF-α： 1.18±0.07 vs 0.66 ±0.08, 0.86 ±0.43, P 〈 0.01, P 〈 0.05）, whereas serum adiponectin and IL-10 decreased in model groups compared with that in the control （adiponectin： A： 21.87±4.84 and B： 21.48 ±4.60 vs 27.36 ±7.29, P 〈 0.05. IL-10： A： 1.72± 0.38 and B： 1.83 ±0.39 vs 2.26±0.24, P 〈 0.01）. Lg （TC） and the degree of liver fatty infiltration was an independent determi

关键词： Nonalcoholic steatohepatitis Pediatricanimal model Adiponectin Interleukin 6 Tumornecrosis factor

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Optical coherent tomography angiography observation of macular subinternal limiting membrane hemorrhage in CO poisoning: a case report

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International Journal of Ophthalmology(English edition) 2022年第11期15卷 1885-1887页

作者： Heng-Di Zhang Ling Zhang Fei Han Wei Jiang fen lan Department of Ophthalmology of the General Hospital of Western Theater Command Chengdu 610083Sichuan ProvinceChina Department of Radiotherapy of the General Hospital of Western Theater Command Chengdu 610083Sichuan ProvinceChina

Dear Editor,I am Heng-Di Zhang,from the Department of Ophthalmology of the Geberal Hospital of Western Theater Command.I am writing to present a case report on optical coherence tomography angiography(OCTA)observation... 详细信息

关键词： limiting coherent monoxide

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Numerical simulation analysis on multi-layer low-temperature heating method of asphalt pavement in hot in-place recycling

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Journal of Central South University 2020年第12期27卷 3793-3806页

作者： MA Deng-cheng lan fen National Engineering Laboratory for Road Maintenance Equipment Chang’an UniversityXi’an 710064China Xi’an Municipal Facilities Management Center Xi’an 710016China

Asphalt mixture pavement reheating is one of the important steps in hot in-place recycling(HIR).To improve the heating speed of asphalt pavement in HIR,based on the numerical analysis model of asphalt mixture heating process,a new multi-layer low-temperature heating method(MLHM)was *** input heat flux,the thermal capacity and thermal resistance of asphalt mixture,the heat transfer model was established based on energy conservation *** heating the asphalt mixture in layers,it changes the situation that the heat energy can only be input from the upper surface of the asphalt mixture *** the simulation of the heating method of asphalt mixture in the existing technology,the result shows that the existing heating methods lead to serious aging or charring of the asphalt *** MLHM,the upper and the bottom of the asphalt mixture are heated at the same time,and the heating temperature is lower than other heat methods,which not only reduces the heating thickness and increases the heating area of the asphalt mixture pavement,but also improves the heating speed,saves the energy resource and ensures the heating ***,by MLHM,the heating uniformity is better and speed is faster.

关键词： asphalt pavement hot in-place recycling heating speed heating uniformity multi-layer low-temperature

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Cloning of the OAT gene and the correlation between its expression and drought tolerance in Phaseolus vulgaris L.

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Journal of Integrative Agriculture 2016年第5期15卷 973-982页

作者： CHEN Ji-bao CAO Yuan-nan ZHANG Zhao-yuan WANG Shu-min WU Jing WANG lan-fen School of Life Science and Technology Nanyang Normal University Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Drought stress is a major abiotic stress of common bean（Phaseolus vulgaris L.） throughout the world. Increasing the proline accumulation contributes to enhance crop drought tolerance. A c DNA for δ-o rnithine aminotransferase（δ-OAT）, an enzyme involved in the biosynthesis of proline, was isolated from Phaseolus vulgaris（Pv OAT）. Pv OAT exhibits 87.4 and 39.8% similarity of the deduced amino acid sequences with δ-OAT from Glycine max and Vigna aconitifolia, respectively. The transcriptional analysis revealed that Pv OAT was strongly induced by drought stress. And the expression of Pv OAT was higher in leaves than that in the root and stem of common bean by drought stress. Similar increase of the proline accumulation was observed in leaves and roots of common bean by drought stress. Furthermore, the proline content, the Pv OAT expression and the Pv OAT enzyme activity in cul tivar F5575 was significantly（P〈0.01） higher than that in cultivar F4851 under drought-stress conditions. Interestingly, it had been observed that, in the later stage of drought stress, the proline steadily maintained at the maximum level maybe result from the Pv OAT enzyme activity increasing steadily. These r esults indicated that the expression of Pv OAT and the accumulation of proline induced by drought stress treatment were related to the degree of common bean drought tolerance. So our results support the view that δ-OAT is associated with proline synthesis under drought stress conditions.

关键词： common bean ornithine aminotransferase gene proline drought tolerance

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Neuronal Activity in the Cerebellum During the Sleep-Wakefulness Transition in Mice

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Neuroscience Bulletin 2020年第8期36卷 919-931页

作者： Li-Bin Zhang Jie Zhang Meng-Jia Sun Hao Chen Jie Yan fen-lan Luo Zhong-Xiang Yao Ya-Min Wu Bo Hu Department of Physiology College of Basic Medical SciencesArmy Medical UniversityChongqing 400038China State Key Laboratory of Trauma Bums and Combined InjuryDaping Hospital/Research Institute of SurgeryArmy Medical UniversityChongqing 400042China Squadron 10 Battalion 3College of Basic Medical SciencesArmy Medical UniversityChongqing 400038China

Cerebellar malfunction can lead to sleep disturbance such as excessive daytime sleepiness,suggesting that the cerebellum may be involved in regulating sleep and/or ***,understanding the features of cerebellar regulation in sleep and wakefulness states requires a detailed characterization of neuronal activity within this *** performing multiple-unit recordings in mice,we showed that Purkinje cells(PCs)in the cerebellar cortex exhibited increased firing activity prior to the transition from sleep to ***,the increased PC activity resulted from the inputs of lowfrequency non-PC units in the cerebellar ***,the increased PC activity was accompanied by decreased activity in neurons of the deep cerebellar nuclei at the nonrapid eye-movement sleep-wakefulness *** results provide in vivo electrophysiological evidence that the cerebellum has the potential to actively regulate the sleep-wakefulness transition.

关键词： Sleep Wakefulness Multiple-unit recording Purkinje cell Deep cerebellar nuclei

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Application of bone transgenic zebrafish in anti-osteoporosis chemical screening

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Animal Models and Experimental Medicine 2018年第1期1卷 53-61页

作者： Hong-xin Huang Hao Lin fen lan Yong-fu Wu Zhen-guo Yang Jing-jing Zhang Affiliated Hospital of Guangdong Medical University The Central People's Hospital of Huizhou

Background: The zebrafish(Danio rerio) has recently been shown to be an ideal model to study bone disease including osteoporosis. The zebrafish osteoporosis model could be induced by glucocorticoid treatment with chemical staining for reflecting the level of bone mineralization. However, this methodology was ***, we developed a novel methodology to directly evaluate the bone mass and ***: We generated and used the bone of transgenic zebrafish Tg(***7:nlsGFP) to evaluate the bone mass and density by measuring the areal extent and the integrated optical density(IOD) of enhanced green fluorescent protein(eGFP).This methodology was further compared with the traditional chemically stained method showing the bone mineralization. Furthermore, genes related to zebrafish osteoporosis were analyzed by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results: Our results of new methods were consistent with those from chemically stained fish, following glucocorticoid-induction or epimedium flavonoid(FE)-rescue treatments. q RT-PCR analyses on mRNA levels revealed that glucocorticoid induces osteoporosis by downregulating the expression of osteoblast-related factors osterix,osteocalcin, and osteopontin, and upregulating the expression of osteoclast-related factor tartrate-resistant acid phosphatase. In FE-rescued fish, the expression of osteogenic factors osterix, osteocalcin, and osteopontin were ***: Compared to the traditional chemical staining methods, the new osteoporosis model using Tg(***7:nlsGFP) is more convenient and efficient for studying osteoporosis in vivo, and especially for high-throughput anti-osteoporosis drug screening.

关键词： drug screening osteoporosis transgenic zebrafish

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A novel confidence estimation method for heterogeneous implicit feedback

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Frontiers of Information Technology & Electronic Engineering 2017年第11期18卷 1817-1827页

作者： Jing WANG lan-fen LIN Heng ZHANG Jia-qi TU Peng-hua YU College of Computer Science and Technology Zhejiang University Hangzhou 310027 China

Implicit feedback, which indirectly reflects opinion through user behaviors, has gained increasing attention in recommender system communities due to its accessibility and richness in real-world applications. A major way of exploiting implicit feedback is to treat the data as an indication of positive and negative preferences associated with vastly varying confidence levels. Such algorithms assume that the numerical value of implicit feedback, such as time of watching, indicates confidence, rather than degree of preference, and a larger value indicates a higher confidence, although this works only when just one type of implicit feedback is available. However, in real-world applications, there are usually various types of implicit feedback, which can be referred to as heterogeneous implicit feedback. Existing methods cannot efficiently infer confidence levels from heterogeneous implicit feedback. In this paper, we propose a novel confidence estimation approach to infer the confidence level of user preference based on heterogeneous implicit feedback. Then we apply the inferred confidence to both point-wise and pair-wise matrix factorization models, and propose a more generic strategy to select effective training samples for pair-wise methods. Experiments on real-world e-commerce datasets from *** show that our methods outperform the state-of-the-art approaches, consid- ering several commonly used ranking-oriented evaluation criteria.

关键词： Recommender systems Heterogeneous implicit feedback Confidence Collaborative filtering E-commerce

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国内药学领域2010-2019年SCI论文的文献计量分析

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药学服务与研究 2020年第3期20卷 217-221页

作者：兰芬海军军医大学长海医院药学部上海200433

目的:利用ESI和InCites数据库,对国内药学领域2010-2019年产出的SCI论文的文献计量指标进行分析,以了解国内药学领域近10年的发展情况和发展趋势。方法:在ESI和InCites数据库中,设定论文发表时间为2010-2019年,机构地域为“中国大陆”(C... 详细信息

目的:利用ESI和InCites数据库,对国内药学领域2010-2019年产出的SCI论文的文献计量指标进行分析,以了解国内药学领域近10年的发展情况和发展趋势。方法:在ESI和InCites数据库中,设定论文发表时间为2010-2019年,机构地域为“中国大陆”(China's Mainland),学科领域为“药理学和药学”(pharmacology and pharmacy),检索国内研究者在2010-2019年发表的药学领域的SCI论文。统计论文发表年份、发表期刊、作者机构所在地域、国际合作情况、基金资助情况等,检索并分析高影响力论文。结果:国内药学领域2010-2019年发表的SCI论文共81679篇,国际合作论文13720篇,高影响力论文470篇。发文量、国际合作论文数和高影响力论文数均逐年增加,与129个国家/地区产生国际合作。48.49%的论文得到国内科研基金的资助。结论:近10年来,国内药学领域的SCI论文产出量持续增加,研究规模在扩大,学术影响力不断上升,国际合作论文和高影响力论文数量增加,但在论文质量和国际合作方面还需进一步提升。

关键词：药学文献计量 SCI论文国际合作

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