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Characterization of blood flow and the effects of exogenous estradiol benzoate on residual follicles formed after ultrasound-guided transvaginal follicle aspiration in cattle

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Journal of Animal Science and Biotechnology 2017年第1期8卷 51-56页

作者： Alberto Mansur Ghetti Luiz Gustavo Bruno Siqueira Eduardo Kenji Nunes Arashiro Miller Pereira Palhao felipe zandonadi brandao Joao Henrique Moreira Viana Universidade Federal Fluminense Niteroi RJ 24230-340 Brazil Embrapa Gado de Leite Juiz de Fora MG 36038-330 Brazil Universidade Jose do Rosario Vellano Alfenas MG 37130-000 Brazil Embrapa Recursos Geneticos e Biotecnologia PqEB Av. W5 N Brasilia DF 70770-917 Brazil

Background： Removal of the follicular content by ultrasound-guided transvaginal follicle aspiration（TVFA） may fail to induce immediate atresia and loss of function, resulting in the occurrence of residual follicles（RF）. The aims of this study were to characterize the blood flow in RF and to determine the effects of the treatment with estradiol benzoate on RF fate. Lactating, cyclic Holstein-Gir crossbred cows were used. In Experiment 1, follicular wave emergence（D0） was synchronized in cows（n = 10） and follicular growth was then monitored by transrectal ultrasonography from D0 to D8, followed by TVFA of the largest follicle present on the ovaries 24 h later. Color Doppler ultrasound imaging was used to examine blood flow on the follicular wall, which was recorded immediately before and every 12 h after TVFA, up to 72 h. In experiment 2, cows（n = 22） were randomly allocated to receive either 2 mL of saline i.m.（Control group, n = 11） or 2 mL estradiol benzoate i.m.（EB group, n = 11）immediately after TVFA. Ovaries were scanned every 12 h to confirm the presence and to measure the diameter of RF. The contents of the RF, if present, were collected 72 h after the first TVFA, using the same aspiration *** fluid from original follicles and RF were stored at-20 °C until hormonal ***： In Experiment 1, there was no reduction（P 〉 0.05） of blood flow in the remaining follicle walls after TVFA and maximum blood flow values were observed at 49.5 ± 19.7 h post-TVFA. In Experiment 2, formation of RF after TVFA was proportionally similar between Controls（5/9） and EB（5/10） cows. Also, RF diameter did not differ between groups（P 〉 0.05）. Nonetheless, the content of RF from cows in the EB group had lower（P = 0.0004）estradiol（E2） concentration and lower（P = 0.0005） E2：P4 ratio compared with ***： In conclusion, 1） the persistence of vascularization in the remaining follicle wall may contribute to the formation of RF after follicle aspiration,

关键词： Doppler Ovary Ovum pick-up Steroidogenesis Vascularization

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