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Dry Matter Production and Nutritional Status of Sunflower Grown in Nutrient Solution under Macronutrient Omission

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Agricultural Sciences 2018年第11期9卷 1479-1486页

作者： Allan Nunes Alves felipe guedes de souza Lúcia Helena Garófalo Chaves Ana Carolina Feitosa de Vasconcelos department of Agricultural Engineering Federal University of Campina Grande Campina Grande Brazil

The objective of this study was to evaluate the effect of omission of macronutrients on dry matter production and on the nutritional status of sunflower cv. BRS 122. The experiment was carried out in a greenhouse, in randomized blocks, with seven treatments corresponding to the Hoagland & Arnon standard solution (1950) and the individual omission of N, P, K, Ca, Mg and S, in three replicates. After 50 days of sowing, the dry matter and the nutritional status of the plants were evaluated. The omission of N, P, K, Ca, Mg and S limited the dry mass production of sunflower cv. BRS 122 in relation to the complete solution, besides causing nutritional imbalance due to the interaction between the nutrients. The omission of the macronutrients K, Ca, and Mg caused the higher imbalances in the plants, highlighting the elevated N contents in these treatments comparing to the complete solution.

关键词： Helianthus annuus Nutritional Diagnosis Omission of Nutrients

Biochar on Soil Chemical Properties and Beak Pepper (<i>Capsicun chinense</i>) Production

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Agricultural Sciences 2020年第12期11卷 1133-1142页

作者： Gilvanise Alves Tito Lúcia Helena Garófalo Chaves Edilma Rodrigues Bento Dantas Laysa Gabryella de souza Laurentino felipe guedes de souza Hugo Orlando Carvallo Guerra department of Agricultural Engineering Federal University of Campina Grande (DEAG/UFCG) Campina Grande Brazil

The transformation and recycling of poultry litter through the pyrolysis process produces a co-product called biochar which, applied to the soil, improves the chemical characteristics of the soil and is used as a soil fertilizer due to its high content of nutrients to plants. The objective of this study was to assess the effect of biochar, produced from poultry litter wastes on soil chemical properties and the culture of the beak pepper (Capsicun chinense). For this, the experimental units, corresponding to plastic vessels, were prepared with 5 kg of soil mixed with increasing doses of biochar: 0;2.5;5.0;7.5 and 10 t∙ha−1, with three replications. This material (soil + biochar) was incubated for 20 days keeping the humidity close to the field capacity. After this period, soil samples from the experimental units were collected and then chemically analyzed. The seedlings of pepper were produced in a greenhouse and transplanted to the experimental units when the seedlings were 15 cm tall. After 10 days of transplanting, thinning was done, leaving a plant/vessel. Biometric variables were analyzed at 120 DAS, after fruit harvesting: plant height (PH), stem diameter (SD), fresh shoot biomass (FSB), fresh fruit biomass (FFB), dry biomass of the aerial part (DSB) and the fruits (DFB). The results were submitted to analysis of variance and regression by orthogonal polynomials using the statistical program SISVAR. Biochar improved the soil chemical properties using as an acidity corrective and source of nutrients, mainly phosphorus and potassium. The doses used, in general, favored the development and production of the beak pepper, increasing the fresh shoot biomass, the fresh and dry fruit biomass. To improve the soil chemical properties, the highest biochar dose is recommended, that is, 10 ton∙ha

Melon Seedlings Phytomass under Poultry Litter Biochar Doses

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Agricultural Sciences 2021年第3期12卷 181-197页

作者： Laysa Gabryella de souza Laurentino Lúcia Helena Garófalo Chaves Antônio Ramos Cavalcante Jean Pereira Guimarães felipe guedes de souza Washington Benevenuto de Lima Hugo Orlando Carvallo Guerra Josely Dantas Fernandes department of Agricultural Engineering Federal University of Campina Grande (DEAG/UFCG) Campina Grande Brazil

The use of organic waste for the preparation of seedling substrates is an important environmental and economic option. In this perspective, substrates using biochar emerges as an alternative for seedling production due to their favorable physical and chemical characteristics. The present study aimed to evaluate the efficiency of doses of poultry litter biochar as a substrate constituent for the production and quality of the seedlings. The work was conducted in a semi protected environment, belonging to the Federal University of Campina Grande—UFCG. The statistical design used was completely randomized in a 6 × 2 factorial scheme, consisting of 6 doses of biochar (0, 4, 8, 12, 16 and 20 t∙ha−1) and two varieties of melon (Yellow and Hales Best Jumbo) with 4 repetitions totaling 48 experimental units. The fresh and dry plant phytomass mass (aerial, roots and total), root length and the quality of seedlings were evaluated. It was concluded that the addition of poultry litter biochar to the substrate was beneficial, promoting an increase in the analyzed seedling variables, being the ideal dose for good development of melon seedlings 12 t∙ha−1. The Yellow variety presented a better response than the Hales Best Jumbo to the charcoal application. Considering the advantages of the use of poultry litter biochar on the substrate composition, found in the present study, its utilization constitutes a viable alternative for the development of melon seedlings and for the environmental disposal of the poultry litter.

关键词： Biochar Cucumis melo L. Nutrition Alternative Substrate

Growth of Pepper under Different Concentration of the Solutions Used in the Hydroponic System

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Agricultural Sciences 2018年第8期9卷 925-935页

作者： felipe guedes de souza Guilherme de Freitas Furtado Antonio Ramos Cavalcante Lucia Helena Garófalo Chaves Josely Dantas Fernandes José Amilton Santos Júnior department of Agricultural Engineering Federal University of Campina Grande Campina Grande Brazil department of Agricultural Engineering Federal Rural University of Pernambuco Recife Brazil

Water availability is a fundamental condition for sustainable agriculture. However, a satisfactory water condition is not always possible in terms of quantity and quality in certain regions, such as the Brazilian semi-arid region. Thus, the purpose of this extremely important research was to evaluate the growth of cv. All Big with six combinations of wastewater plus saline (WW + SW) and two nutrient solution percentages, in a low-cost hydroponic system. The experiment was carried out in a greenhouse at the department of Agricultural Engineering at Federal University of Campina Grande. A completely randomized experimental design was used, with treatments arranged in a factorial scheme (6 × 2), with 5 replications, being the treatments composed by the combination of six proportions of wastewater with saline water (50%/50%;60%/40%;70%/30%;80%/20%;90%/10%;100% of wastewater) and two nutrient solution concentration (80% and 100%), totalizing 60 experimental units. The biometric variables were analyzed at 55 and 96 days after sowing, namely: plant height (PH), stem diameter (SD), leaf number (LN) and leaf area (LA). The interaction between the concentration of the nutrient solution and the ratio of the saline water with wastewater was influenced in the behavior of peppers (cv. All Big) biometric variables. The nutrient solution prepared at 80% of its normal concentration had better performance in the development of plants when mixed with the proportions of water containing more wastewater. The proportion 30% saline water and 70% wastewater (30/70) with concentration of 80% of the nutrient solution obtained better satisfactory results in the studied biometric variables.

关键词： Capsicum annuum L. Saline Water Wastewater Nutrient Solution

Effect of rare-earth oxides on properties of silicon nitride obtained by normal sintering and sinter-HIP

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Journal of Rare Earths 2012年第11期30卷 1177-1183页

作者： Cecilia C.guedes-Silva Flávio Machado de souza Carvalho José Carlos Bressiani Nuclear and Energy Research Institute-IPEN/CNEN Geoscience Institute-IGC/USP

This paper presented the microstructure, mechanical properties and oxidation behavior of silicon nitride obtained by both conventional sintering and sintering followed by hot isostatic pressing （HIP）. Silicon nitride with additives such as 5 wt.% Al2O3 and 5 wt.% Ln203 （Ln= La, La concentrate, Gd or La＋Gd） were studied. The results revealed that Gd203 additions increased the formation of elongated grains of β-Si3N4, the fracture toughness and oxidation resistance. La2O3 additions led to higher densification and hardness values, while addition of La2O3 concentrate promoted the formation of materials with intermediate properties, compared to the other studied compositions. Hot isostatic pressing increased the hardness but decreased the fracture toughness of the material, mainly because it allowed residual pores to close and also reduced the average aspect ratio of β-Si3N4 grains.

关键词： rare earths silicon nitride properties normal sintering sinter-HIP

Biofilm analyses and exoproduct release by clinical and environmental isolates of Burkholderia pseudomallei from Brazil

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Asian Pacific Journal of Tropical Medicine 2023年第7期16卷 321-328页

作者： Glaucia Morgana de Melo guedes Crister JoséOcadaque Alyne Soares Freitas Rodrigo Machado Pinheiro Giovanna Barbosa Riello Silviane Praciano Bandeira Rossana de Aguiar Cordeiro Marcos Fábio Gadelha Rocha JoséJúlio Costa Sidrim Débora de souza Collares Maia Castelo-Branco department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyGroup of Applied Medical MicrobiologyFederal University of CearáFortalezaCEBrazil department of Clinical and Toxicological Analysis School of PharmacyFederal University of CearáFortalezaCEBrazil department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyLaboratory of Emerging and Reemerging PathogensFederal University of CearáFortalezaCEBrazil School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of CearáFortalezaCEBrazil

Objective:To characterize biofilm production by clinical(n=21)and environmental(n=11)isolates of Burkholderia pseudomallei and evaluate the production of proteases,hemolysins and ***:Initially,the 32 strains were evaluated for biofilm production in Müller-Hinton broth-1%glucose(MH-1%glucose)and BHI broth-1%glucose,using the crystal violet staining ***,growing(48 h)and mature(72 h)biofilms were evaluated by confocal ***,the production of proteases,hemolysins and siderophores by planktonic aggregates,growing biofilms and mature biofilms was ***:All isolates produced biofilms,but clinical isolates had significantly higher biomass in both MH-1%glucose(Pderophores,mature biofilms presented higher production than growing biofilms(Pderophores seem important for growth and maintenance of Burkholderia pseudomallei biofilms.

关键词： Mangosteen Burkholderia pseudomallei Biofilms Proteases Hemolysins Siderophores

Buzz-pollination in Neotropical bees:genus-dependent frequencies and lack of optimal frequency for pollen release

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Insect Science 2020年第1期27卷 133-142页

作者： Conrado Augusto Rosi-denadai Priscila Cassia souza Araujo Lucio Antonio de Oliveira Campos Lirio Cosme Jr. Raul Narciso Carvalho guedes departamento de Entomologia Universidade Federal de VigosaVigosaMinas GeraisBrazil departamento de Biologia Geral Universidade Federal de VigosaVigosaMinas GeraisBrazil

Over 50 genera of bees release pollen from flower anthers using thoracic vibrations,a phenomenon known as *** efficiency of this process is directly affected by the mechanical properties of the buzzes,namely the duration,amplitude,and ***,although the effects of the former two properties are well described,the role of buzz frequency on pollen release remains ***,nearly all of the existing studies describing vibrational properties of natural buzz-pollination are limited to bumblebees(Bombus)and carpenter bees(Xvlocopa)constraining our current understanding of this behavior and its ***,we attempted to minimize this shortcoming by testing whether flower anthers exhibit optimal frequency for pollen release and whether bees tune their buzzes to match these(optimal)*** true,certain frequencies will trigger more pollen release and lighter bees will reach buzz frequencies closer to this optimum to compensate their smaller buzz *** strategies were used to test these hypotheses:(i)the use of(artificial)vibrational playbacks in a broad range of buzz frequencies and amplitudes to assess pollen release by tomato plants(Solarium Ivcopersicum L.)and(ii)the recording of natural buzzes of Neotropical bees visiting tomato plants during *** playback experiment indicates that although buzz frequency does affect pollen release,no optimal frequency exists for *** addition,the recorded results of natural buzz-pollination reveal that buzz frequencies vary with bee genera and are not correlated with body ***,neither bees nor plants are tuned to optimal pollen release *** frequency of buzz-pollination is a likely consequence of the insect flight machinery adapted to reach higher accelerations,while flower plant response to buzz-pollination is the likely result of its pollen granular properties.

关键词： buzz amplitude buzz frequency floral sonication pollination stingless bees vibration

Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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World Journal of Gastroenterology 2012年第31期18卷 4162-4168页

作者： Raimundo Fernandes de Araújo Júnior Luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino Hugo Gon alo guedes Pedro Ros Petrovick Tatiane Pereira de souza Aurigena Antunes de Araújo Gerlane Coelho Bernardo Guerra department of Morphology/Postgraduate Program in Morphological Sciences Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PernambucoRecife 50670-901PernambucoBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Rio Grande do NorteNatal 59012-570Rio Grande do NorteBrazil department of Production and control of drugs Federal University of Rio Grande do SulPorto Alegre 90610-000Rio Grande do SulBrazil department of Food and Drug Administration Federal University of AmazonasManaus 69077-000AmazonasBrazil department of Biophysics and Pharmacology Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil

AIM:To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell ***:Colorectal carcinoma(HT29) and human hepatocellular carcinoma(HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri(SdePN) either alone or in combination with cisplatin at different concentrations(0.5 mg/mL and 1 mg/mL) for 4 h and 24 *** verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability,we stained the cells with propidium iodide and assessed them by flow *** percentage of cells in different cell cycle phases was quantified and data were expressed as *** differences between groups were determined using analysis of variance and Bonferroni's test,as indicated.A value of P dered to be statistically ***:SdePN had significantly different cytotoxic effects on HT29(2.81 ± 0.11 vs 3.51 ± 1.13,P > 0.05) and HepG2(5.07 ± 0.3 vs 15.9 ± 1.04,P 0.05) and HepG2(14.56 ± 1.6 vs 35.67 ± 3.94,P dependent manner compared to control cells(HepG2 cells for 4 h:10.78 ± 1.58 vs 53.89 ± 1.53,P dePN and subsequent treatment with cis-platin resulted in a greater number of cells being killed(12.78 ± 1.01 vs 93.76 ± 1.6,P dePN when combined with cisplatin(12.87 ± 2.78 vs 78.8 ± 3.02,P dePN is selectively toxic against two cancer cell ***,SdePN in combinati

关键词：肿瘤细胞株提取物生长抑制作用顺铂珍珠 HepG2细胞细胞毒作用喷雾干燥

Vibrio *** Macrobrachium amazonicum prawn farming are inhibited by Moringa oleifera extracts

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Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 899-902页

作者： Raimunda Samia Nogueira Brilhante Jamille Alencar Sales Celia Maria de souza Sampaio Francisco Geraldo Barbosa Manoel de Araujo Neto Paiva Glaucia Morgana de Melo guedes Lucas Pereira de Alencar Yago Brito de Ponte Tereza de Jesus Pinheiro Gomes Bandeira Jose Luciano Bezerra Moreira debora de souza Collares Maia Castelo-Branco Waldemiro de Aquino Pereira-Neto Rossana de Aguiar Cordeiro Jose Julio Costa Sidrim Marcos Fabio Gadelha Rocha department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceara School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceara department of Organic and Inorganic Chemistry Federal University of Ceara

Objective:To investigate the in vitro antimicrobial potential of extracts of stem,leaves,flowers,pods and seeds of Moringu okifera(***) against Vibrio *** hatchery water and the prawn Mmrobrachium ***:The ethanol extracts of stem,leaves,pods and seeds and chloroform extract of flowers of *** were tested against Vibrio cholerue(***) serogroups non-O1/non-O139(n=4).Vibrio vulnificus(n=l) and Vibrio mimicus(n=1).Escherichia coli(***)(ATCC(?) 25922) was used as quality *** species were obtained from Macmbrachium amazonicum prawns and from hatchery water from prawn *** Minimum Inhibitory Concentration(MIC) was determined by broth microdilution ***:The best result was obtained with the ethanol extract of pods,which inhibited three strains of the ***,Vibrio vulnificus,Vibrio minimis and ***(MIC range 0.312-5.000 mg/mL).The chloroform extract of flowers was effective against all *** strains and ***(MIC range 0.625-1.250 mg/mL).However,the ethanol extracts of stem and seeds showed low effectiveness in inhibiting the bacterial ***:The extracts of pods,flowers and leaves of *** have potential for the control of Vibrio *** studies are necessary to isolate the bioaetive compounds responsible for this antimicrobial activity.

关键词： Prawn Macrobrachium amazonicum Moringa oleifera Vi