检索结果-南通市图书馆

Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2021年第4期14卷 556-570页

作者： Mengjiao chen Weijuan Fan feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin chen Jie Qiu Xin Wei Ziru chen Peng Zhang Dong Pei Jun Yang Xuehui Huang Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai Chenshan Botanical GardenShanghai 201602China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Tai'an Academy of Agricultural Sciences Tai'an 271000ShandongChina National Genomics Data Center Bio-Med Big Data CenterCAS Key Laboratory of Computational BiologyCAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China CAS Center for Excellence of Molecular Plant Sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China These authors contributed equally to this article.

Many important crops(e.g.,tuber,root,and tree crops)are *** these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid *** this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing *** applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic *** causative genes at these loci were detected based on functional *** together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient *** OutcrossSeq software and its instruction manual are available for downloading at ***/tool/***.

关键词： genotyping genetic mapping outcrossing polyploid

Promoting interactions between cognitive science and large language models

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Innovation 2024年第2期5卷 9-10页

作者： Youzhi Qu Penghui Du Wenxin Che chen Wei Chi Zhang Wanli Ouyang Yatao Bian feiyang Xu Bin Hu Kai Du Haiyan Wu Jia Liu Quanying Liu Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen 518055China Shanghai AI Laboratory Shanghai 200232China Tencent AI Lab Shenzhen 518057China iFLYTEK AI Research Hefei 230088China School of Medical Technology Beijing Institute of TechnologyBeijing 100081China Institute for Artificial Intelligence Peking UniversityBeijing 100871China Centre for Cognitive and Brain Sciences and Department of Psychology University of MacaoMacao 999078China Department of Psychology Tsinghua UniversityBeijing 100084China

Large language models(LLMs)have made unprecedented progress,demonstrating human-like language proficiency and an extraordinary ability to encode complex *** emergence of high-level cognitive capabilities in LLMs,such as in-context learning and complex reasoning,suggests a path toward the realization of artificial general intelligence(AGI).However,we lack scientific theories and tools to assess and interpret such an emergence of the advanced intelligence of *** intelligence(AI)has been extensively applied in various areas of fundamental science to accelerate scientific research.

关键词： cognitive such language

A potent neutralizing antibody provides protection against SARS-CoV-2 Omicron and Delta variants via nasal delivery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第9期7卷 3584-3591页

作者： Xinghai Zhang Huajun Zhang Tingting Li Shaohong chen feiyang Luo Junhui Zhou Peiyi Zheng Shuyi Song Yan Wu Tengchuan Jin Ni Tang Aishun Jin chengyong Yang Guofeng cheng Rui Gong Sandra Chiu Ailong Huang CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430o71 HubeiChina Department of Immunology College of Basic MedicineChongqing Medical UniversityChongqing 400010China Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology Chongqing Medical UniversityChongqing 400010China University of Chinese Academy of Sciences 100049 BeijingChina Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230026 AnhuiChina Key Laboratory of Molecular Biology on Infectious Diseases Ministry of EducationChongqing Medical UniversityChongqing 400010China Mindao Haoyue Co LtdSQ0042 Lanyuan HallNo.61 Daxuecheng Middle RoadChongqing 400042China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still rapidly spreading *** drugs and vaccines have been approved for clinical use show efficacy in the treatment and prevention of SARS-CoV-2 ***,the emergence of SARS-CoV-2 variants of concern(VOCs),such as Delta(B.1.617.2)and the recently emerged Omicron(B.1.1.529),has seriously challenged the application of current ***,there is still a pressing need for identification of new broad-spectrum ***,we further characterized a human antibody(58G6),which we previously isolated from a patient,with a broadly authentic virus-neutralizing activity that inhibits the Delta and Omicron variants with half-maximal inhibitory concentrations(ICso)of 1.69 ng/ml and 54.31 ng/ml,respectively.58G6 shows prophylactic and therapeutic effcacy in hamsters challenged with the Delta and Omicron variants through nasal ***,a very low dosage(2 mg/kg daily)of 58G6 efficiently prevented Omicron variant replication in the *** advantages may overcome the efficacy limitation of currently approved neutralizing antibodies that can be administered only by intravenous *** general,58G6 is a promising prophylactic and therapeutic candidate against current circulating VOCs and even future emerging *** the best of our knowledge,58G6 is one of the most potent neutralizing antibodies against Omicron,with a broader spectrum than those approved for clinical use.58G6 could be developed as a nebulized therapy,which would be more cost effective and user friendly and enhance the clinical outcome comparedto thatobtainedwithdirect nasaldelivery.

关键词： nasal dosage prevention

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

民营企业身份认同与审计师选择

会计研究 2023年第8期 164-178页

作者：陈丽蓉高菲阳西南政法大学商学院 401120

民营企业是推动质量强国建设、促进经济社会发展和共同富裕的重要力量,民营企业身份认同问题深刻影响其审计师选择的经营行为。本文以2003-2021年我国沪深A股非金融类上市民营企业数据为样本,考察民营企业身份认同问题对审计师选择的影... 详细信息

民营企业是推动质量强国建设、促进经济社会发展和共同富裕的重要力量,民营企业身份认同问题深刻影响其审计师选择的经营行为。本文以2003-2021年我国沪深A股非金融类上市民营企业数据为样本,考察民营企业身份认同问题对审计师选择的影响及其作用途径。研究发现,身份认同问题降低民营企业对高质量审计师的选择意愿,且短视行为、经营风险和额外成本在其中发挥部分中介作用。进一步研究发现,这一影响关系在市场化水平低的地区、《关于完善产权保护制度依法保护产权的意见》颁布前更为显著。本文从民营企业身份认同问题这一新视角拓展审计师选择的相关研究,厘清民营企业身份认同问题与审计师选择之间的作用机理,为推动政府依法保护民营企业产权和企业家权益、提升民营企业经济绩效,促进我国经济高质量发展提供经验证据和政策参考。

关键词：国有转制型民营企业创业型民营企业身份认同审计师选择

Efficient Large Language Model Application Development: A Case Study of Knowledge Base, API, and Deep Web Search Integration

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Computer and Communications 2024年第12期12卷 171-200页

作者： Xiangyu Wang Yan Tan Tao Yang Meng Yuan Shaohan Wang Min chen feiyang Ren Zijian Zhang Yuqi Shao COSCO SHIPPING Technology Co. Ltd. Shanghai China COSCO SHIPPING Specialized Carriers Co. Ltd. Guangzhou China

This paper presents a reference methodology for process orchestration that accelerates the development of Large Language Model (LLM) applications by integrating knowledge bases, API access, and deep web retrieval. By incorporating structured knowledge, the methodology enhances LLMs’ reasoning abilities, enabling more accurate and efficient handling of complex tasks. Integration with open APIs allows LLMs to access external services and real-time data, expanding their functionality and application range. Through real-world case studies, we demonstrate that this approach significantly improves the efficiency and adaptability of LLM-based applications, especially for time-sensitive tasks. Our methodology provides practical guidelines for developers to rapidly create robust and adaptable LLM applications capable of navigating dynamic information environments and performing effectively across diverse tasks.

关键词： Large Language Model Knowledge Base API Integration Web Retrieval Application Development

Broadly neutralizing antibodies derived from the earliest COVID-19 convalescents protect mice from SARS-CoV-2 variants challenge

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第10期8卷 4914-4926页

作者： Qianyun Liu Haiyan Zhao Zhiqiang Li Zhen Zhang Rui Huang Mengxue Gu Ke Zhuang Qing Xiong Xianying chen Weiyi Yu Shengnan Qian Yuzhen Zhang Xue Tan Muyi Zhang feiyang Yu Ming Guo Zhixiang Huang Xin Wang Wenjie Xiang Bihao Wu Fanghua Mei Kun Cai Limin Zhou Li Zhou Ying Wu Huan Yan heng Cao Ke Lan Yu chen State Key Laboratory of Virology Instute for Vaccine ResearchCollege of Life SciencesWuhan UniversityWuhan 430072China Department of Thoracic Surgery Renmin HospitalWuhan UniversityWuhanChina CAS Key Laboratory of Special Pathogens Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430o71P.R.China Animal Biosafety Level-ll Laboratory/lnstitute for Vaccine Research Wuhan UniversityWuhanChina State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology Institute of Medical VirologyTaikang Medical SchoolWuhan UniversityWuhan 430072China Hubei Center for Disease Control and Prevention Wuhan 430079China Maternal and Child Health Hospital of Hubei Province Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Coronavirus disease 2019(COviD-19)was first reported three years ago,when a group of individuals were infected with the original SARS-CoV-2 strain,based on which vaccines were ***,we develop six human monoclonal antibodies(mAbs)from two elite convalescents in Wuhan and show that these mAbs recognize diverse epitopes on the receptor binding domain(RBD)and can inhibit the infection of SARS-CoV-2 original strain and variants of concern(VOCs)to varying degrees,including Omicron strains XBB and XBB.

关键词： antibodies monoclonal neutral

Cumulative live birth rates of in vitro fertilization/intracytoplasmic sperm injection after multiple complete cycles in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2020年第5期34卷 361-368,I0002-I0006页

作者： Lei Huang Qun Lu Jiangbo Du Hong Lv Shiyao Tao Shiyao chen Xiuzhu Li Xiumei Han Kun Zhou Bo Xu Xiaoyu Liu Hongxia Ma Yankai Xia Guangfu Jin Hongbing Shen Xiufeng Ling Zhibin Hu Jichun Tan feiyang Diao State Key Laboratory of Reproductive Medicine School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Center for Global Health School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Epidemiology School of Public HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Toxicology School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Reproduction the Affiliated Nanjing Maternity and Child Health Hospital of Nanjing Medical UniversityNanjingJiangsu 210004China Center of Reproductive Medicine Department of Obstetrics and GynecologyShengjing Hospital of China Medical UniversityShenyangLiaoning 110022China Department of Reproduction the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

There were few studies of cumulative live birth rates(CLBRs) based on multicenter reproductive clinical data from the general Chinese *** we report a retrospective cohort study,including 14 311 women with17 315 cycles,in three reproductive centers to evaluate two estimated parameters of CLBRs with multiple transfer cycles of in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI) in a Chinese *** found that CLBRs were related to female age and endometrial *** the fourth transfer cycle,the conservative and optimal estimates of CLBRs were 52.95% and 77.30% in women under 30 years of age,and 18.17% and26.51% in those 37 years of age or older,*** two estimates were 44.70% and 63.15% in women with endometrial thickness more than 7 mm,and 32.05% and 46.18% in those with less than 7 mm,*** addition,body mass index(BMI),duration of infertility,and infertility diagnoses may also be related to CLBRs on certain *** findings from this study on CLBRs after multiple transfer cycles of IVF/ICSI treatment on different conditions in the Chinese population should be beneficial to both infertile couples and clinicians.

关键词： cumulative live birth rate in vitro fertilization intracytoplasmic sperm injection

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

蜡样芽孢杆菌分型方法研究进展

食品科学 2017年第17期38卷 286-290页

作者：曹飞扬王娉江连洲陈颖中国检验检疫科学研究院农产品安全研究中心北京100176 东北农业大学食品学院黑龙江哈尔滨150030

蜡样芽孢杆菌是一种食源性条件致病菌,在环境和食品中分布比较广泛,所引起的食物中毒常分为腹泻型和呕吐型。研究蜡样芽孢杆菌的分型方法对该菌的预防、预警、溯源及分子流行病学的调查都具有重要意义。目前,蜡样芽孢杆菌的分型方法主... 详细信息

蜡样芽孢杆菌是一种食源性条件致病菌,在环境和食品中分布比较广泛,所引起的食物中毒常分为腹泻型和呕吐型。研究蜡样芽孢杆菌的分型方法对该菌的预防、预警、溯源及分子流行病学的调查都具有重要意义。目前,蜡样芽孢杆菌的分型方法主要包括噬菌体分型、生化分型等传统分型方法以及多位点序列分型、重复序列聚合酶链式反应分型、随机扩增多态性DNA分型、脉冲场凝胶电泳分型等分子分型方法。以蜡样芽孢杆菌为对象,综述其分型方法的研究进展,以期为该菌的分型提供一定参考。

关键词：蜡样芽孢杆菌传统分型分子分型

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

潜艇静力矩作用下垂直面的操纵运动特性分析

中国舰船研究 2023年第1期18卷 124-140,151页

作者：陈前刘志华骆飞洋海军工程大学舰船与海洋学院武汉430033

[目的]针对潜艇垂直面操舵潜浮机动模式中存在的低航速下艉升降舵变深能力差,以及艉升降舵卡舵和遭遇海水密度断崖时挽回困难的问题,开展静力矩操纵方式的机理性研究。[方法]首先,通过理论分析证明采用静力矩操纵方式可以消除艉升降舵... 详细信息

[目的]针对潜艇垂直面操舵潜浮机动模式中存在的低航速下艉升降舵变深能力差,以及艉升降舵卡舵和遭遇海水密度断崖时挽回困难的问题,开展静力矩操纵方式的机理性研究。[方法]首先,通过理论分析证明采用静力矩操纵方式可以消除艉升降舵的逆速现象;然后,对潜艇采用静力矩和舵水动力操纵方式实施上浮机动过程进行数值模拟。[结果]结果显示,采用静力矩操纵方式能使潜艇在低航速下保持良好的垂直面机动性能,消除艉升降舵的逆速现象,并可避免潜艇在高航速下产生过大的纵倾角;同时,还能改善卡舵和掉深这2种紧急情况下的挽回效果。[结论]采用静力矩操纵方式有利于提高潜艇的机动性和安全性,研究结果可为潜艇的操纵能力设计提供参考。

关键词：潜艇垂直面机动静力矩操纵逆速卡艉升降舵密度断崖