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3D scene graph prediction from point clouds

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Virtual Reality & Intelligent Hardware 2022年第1期4卷 76-88页

作者： Fanfan WU feihu yan Weimin SHI Zhong ZHOU State Key Laboratory of Virtual Reality Technology and Systems School of Computer Science and EngineeringBeihang UniversityBeijing 100191China

Background In this study,we propose a novel 3D scene graph prediction approach for scene understanding from point clouds.Methods It can automatically organize the entities of a scene in a graph,where objects are nodes and their relationships are modeled as edges.More specifically,we employ the DGCNN to capture the features of objects and their relationships in the scene.A Graph Attention Network(GAT)is introduced to exploit latent features obtained from the initial estimation to further refine the object arrangement in the graph structure.A one loss function modified from cross entropy with a variable weight is proposed to solve the multi-category problem in the prediction of object and predicate.Results Experiments reveal that the proposed approach performs favorably against the state-of-the-art methods in terms of predicate classification and relationship prediction and achieves comparable performance on object classification prediction.Conclusions The 3D scene graph prediction approach can form an abstract description of the scene space from point clouds.

关键词： Scene understanding 3D scene graph Point cloud DGCNN GAT

Viral vectored vaccines:design,development,preventive and therapeutic applications in human diseases

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Signal Transduction and Targeted Therapy 2023年第5期8卷 2113-2150页

作者： Shen Wang Bo Liang Weiqi Wang Ling Li Na Feng Yongkun Zhao Tiecheng Wang feihu yan Songtao yang Xianzhu Xia Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research InstituteChinese Academy of Agricultural SciencesChangchunChina College of Veterinary Medicine Jilin UniversityChangchunChina China National Research Center for Exotic Animal Diseases China Animal Health and Epidemiology CenterQingdaoChina

Human diseases,particularly infectious diseases and cancers,pose unprecedented challenges to public health security and the global economy.The development and distribution of novel prophylactic and therapeutic vaccines are the prioritized countermeasures of human disease.Among all vaccine platforms,viral vector vaccines offer distinguished advantages and represent prominent choices for pathogens that have hampered control efforts based on conventional vaccine approaches.Currently,viral vector vaccines remain one of the best strategies for induction of robust humoral and cellular immunity against human diseases.Numerous viruses of different families and origins,including vesicular stomatitis virus,rabies virus,parainfluenza virus,measles virus,Newcastle disease virus,influenza virus,adenovirus and poxvirus,are deemed to be prominent viral vectors that differ in structural characteristics,design strategy,antigen presentation capability,immunogenicity and protective efficacy.This review summarized the overall profile of the design strategies,progress in advance and steps taken to address barriers to the deployment of these viral vector vaccines,simultaneously highlighting their potential for mucosal delivery,therapeutic application in cancer as well as other key aspects concerning the rational application of these viral vector vaccines.Appropriate and accurate technological advances in viral vector vaccines would consolidate their position as a leading approach to accelerate breakthroughs in novel vaccines and facilitate a rapid response to public health emergencies.

关键词： vaccines diseases protective

Nanobodies with cross-neutralizing activity provide prominent therapeutic efficacy in mild and severe COVID-19 rodent models

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Virologica Sinica 2023年第5期38卷 787-800页

作者： Qiuxue Han Shen Wang Zhenshan Wang Cheng Zhang Xinyue Wang Na FengTiecheng Wang Yongkun Zhao Hang Chi feihu yan Xianzhu Xia Institute of Laboratory Animal Sciences Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100021China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun132122China Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou225009China

The weakened protective efficacy of COVID-19 vaccines and antibodies caused by SARS-CoV-2 variants presents a global health emergency,which underscores the urgent need for universal therapeutic antibody intervention for clinical patients.Here,we screened three alpacas-derived nanobodies(Nbs)with neutralizing activity from twenty RBD-specific Nbs.The three Nbs were fused with the Fc domain of human IgG,namely aVHH-11-Fc,aVHH-13-Fc and aVHH-14-Fc,which could specifically bind RBD protein and competitively inhibit the binding of ACE2 receptor to RBD.They effectively neutralized SARS-CoV-2 pseudoviruses D614G,Alpha,Beta,Gamma,Delta,and Omicron sub-lineages BA.1,BA.2,BA.4,and BA.5 and authentic SARS-CoV-2 prototype,Delta,and Omicron BA.1,BA.2 strains.In mice-adapted COVID-19 severe model,intranasal administration of aVHH-11-Fc,aVHH-13-Fc and aVHH-14-Fc effectively protected mice from lethal challenges and reduced viral loads in both the upper and lower respiratory tracts.In the COVID-19 mild model,aVHH-13-Fc,which represents the optimal neutralizing activity among the above three Nbs,effectively protected hamsters from the challenge of SARS-CoV-2 prototype,Delta,Omicron BA.1 and BA.2 by significantly reducing viral replication and pathological alterations in the lungs.In structural modeling of aVHH-13 and RBD,aVHH-13 binds to the receptor-binding motif region of RBD and interacts with some highly conserved epitopes.Taken together,our study illustrated that alpaca-derived Nbs offered a therapeutic countermeasure against SARS-CoV-2,including those Delta and Omicron variants which have evolved into global pandemic strains.

关键词： COVID-19 SARS-CoV-2 Nanobody Broad-spectrum Therapeutic Rodent models

An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

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Virologica Sinica 2023年第2期38卷 244-256页

作者： Haili Zhang Hongli Jin feihu yan Yumeng Song Jiaxin Dai Cuicui Jiao Yujie Bai Jingxuan Sun Di Liu Shen Wang Mengyao Zhang Jilong Lu Jingbo Huang Pei Huang Yuanyuan Li Xianzhu Xia Hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun130062China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun130122China Changchun Sino Biotechnology Co. Ltd.Changchun130012China

Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in animals.Rabies virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health threat.The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and dogs.The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice.Notably,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer periods.Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.

关键词： SARS-CoV-2 Vaccine RBD Chimeric expression RABV

蓝狐细小病毒VP2基因的测序及遗传进化分析

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中国病原生物学杂志 2024年第6期19卷 621-624,630页

作者：曹思凡祁少华王建忠赵永坤闫飞虎冯娜夏咸柱东北农业大学动物医学院黑龙江哈尔滨150036 中国农业科学院长春兽医研究所吉林省人兽共患病预防与控制重点实验室北京协和医学院比较医学中心吉林农业大学动物医学院

目的探究山东省潍坊市蓝狐细小病毒VP2基因的遗传进化特征,了解国内蓝狐细小病毒流行毒株的分子特性。方法根据GenBank公布的BFPV及CPV参考株基因序列设计VP2基因引物,应用细小病毒PCR检测方法对来自山东省潍坊市某蓝狐养殖场的健康和... 详细信息

目的探究山东省潍坊市蓝狐细小病毒VP2基因的遗传进化特征,了解国内蓝狐细小病毒流行毒株的分子特性。方法根据GenBank公布的BFPV及CPV参考株基因序列设计VP2基因引物,应用细小病毒PCR检测方法对来自山东省潍坊市某蓝狐养殖场的健康和腹泻粪便样品进行核酸检测,扩增核酸阳性样品的VP2全基因序列,使用MegAlign软件进行拼接整理,之后借助DNASTAR和MEGA7.0软件构建进化树进行遗传进化分析并完成核苷酸与氨基酸序列分析。结果20份样品中有15份样品(9份腹泻蓝狐样品、6份健康蓝狐样品)均呈细小病毒核酸阳性且扩增出符合预期长度的VP2基因特异性条带。序列同源性比对结果显示,BFPV/WF/2023株与国内CPV-2c参考株同源性最高,可达99.7%~99.9%,与疫苗参考株(CPV-2)同源性为98.7%;与FPV、MEV、BFPV参考株同源性最低,为98.1%~98.4%。系统进化树结果显示,BFPV/WF/2023株与国内CPV-2c参考株处于一个大分支且与BFPV参考株距离较远。蛋白氨基酸分析结果显示,BFPV/WF/2023株与国内CPV-2c参考株关键氨基酸位点(297 A、324 I、370 R、426 E)一致。结论山东省潍坊市健康和腹泻的蓝狐种群中均存在细小病毒感染,且蓝狐细小病毒已演变为犬科的优势毒株CPV-2c亚型,需加强对狐细小病毒感染情况的追踪和监测。

关键词：狐细小病毒 VP2基因基因型遗传进化

我国沿海滩涂盐碱地改良与综合利用现状与展望

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中国草地学报 2024年第2期46卷 1-13页

作者：孙盛楠严学兵尹飞虎扬州大学动物科学与技术学院江苏扬州225000 新疆农垦科学院农田水利与土壤肥料研究所新疆石河子832000

沿海滩涂盐碱地是我国重要的后备土地资源,其生态生产力价值是沿海“蓝碳”的重要组成部分。针对长期以来沿海地区盐碱地综合利用效率低下和经济、生态、社会效益不高等问题,本文通过梳理沿海滩涂开发的历史和现状,总结不同改良方式主... 详细信息

沿海滩涂盐碱地是我国重要的后备土地资源,其生态生产力价值是沿海“蓝碳”的重要组成部分。针对长期以来沿海地区盐碱地综合利用效率低下和经济、生态、社会效益不高等问题,本文通过梳理沿海滩涂开发的历史和现状,总结不同改良方式主导的滩涂盐碱地利用方式的转变,评估滩涂盐碱地的生态生产力价值,分析滩涂盐碱地利用亟需解决的问题,以期为新时期我国沿海滩涂盐碱地的改良与综合利用提供参考。

关键词：滨海地区盐碱地草田轮作生态种养循环土地资源

双能量CT血管造影“点征”联合CT平扫“岛征”预测自发性脑出血早期血肿扩大的临床价值

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当代医学 2022年第19期28卷 132-134页

作者：龚涛晏飞虎陈志勤宜春市人民医院放射科江西宜春336000 宜春市人民医院外科护理江西宜春336000

目的探讨双能量CT血管造影“点征”联合CT平扫“岛征”预测自发性脑出血早期血肿扩大的临床价值。方法选取2021年1—4月本院收治的60例疑似自发性脑出血患者,发病6 h内行首次基线CT+双能量CTA检查,24 h内复查CT平扫。根据两次CT平扫结... 详细信息

目的探讨双能量CT血管造影“点征”联合CT平扫“岛征”预测自发性脑出血早期血肿扩大的临床价值。方法选取2021年1—4月本院收治的60例疑似自发性脑出血患者,发病6 h内行首次基线CT+双能量CTA检查,24 h内复查CT平扫。根据两次CT平扫结果,将患者分为扩大组和无扩大组。分析两组CT平扫“岛征”及双能量CTA“点征”检出率及预测自发性脑出血早期血肿扩大的临床价值,分析自发性脑出血早期血肿扩大的危险因素。结果 60例患者中血肿扩大38例,未扩大22例。扩大组CT平扫“岛征”及双能量CTA“点征”检出率均明显高于未扩大组(P<0.05)。其中“岛征”和“点征”预测敏感度分别为73.68%、55.26%,特异度为68.18%、77.27%,阳性预测值为80.00%、80.77%,阴性预测值为60.00%、50.00%。扩大组患者年龄及酗酒、昏迷和服用阿司匹林者比例均明显高于未扩大组(P<0.05);经Logistic回归分析显示,年龄(≥60岁)、“岛征”及“点征”为预测自发性脑出血早期血肿扩大的独立危险因素(P<0.05)。结论 CT平扫“岛征”及双能量CTA“点征”可有效预测自发性脑出血患者急性脑血肿扩大的风险。

关键词：双能量CTA“点征” CT平扫“岛征” 自发性脑出血血肿扩大

The duration of acute kidney injury is an additional parameter to predict1-year survival in very elderly patients

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Journal of Intensive Medicine 2023年第3期3卷 283-290页

作者： Qinglin Li yan Wang feihu Zhou Department of Critical Care Medicine The First Medical CenterChinese PLA General HospitalBeijing 100853China Department of Cardiology The Second Medical CenterChinese PLA General HospitalBeijing 100853China Chinese PLA General Hospital National Clinical Research Center for Geriatric Diseases Beijing 100853China Medical Engineering Laboratory of Chinese PLA General Hospital Beijing 100853China

Background:Acute kidney injury(AKI)is primarily defined and classified according to the magnitude of theelevation of serum creatinine(Scr).We aimed to determine whether the duration of AKI adds prognostic valuein addition to that obtained from the magnitude of injury alone.Methods:This retrospective study enrolled very elderly inpatients(≥75 years)in the Chinese PLA General Hospitalfrom January 2007 to December 2018.AKI was stratified by magnitude according to KDIGO stage(1,2,and 3)andduration(1–2 days,3–4 days,5–7 days,and>7 days).The primary outcome was the 1-year mortality after AKI.Multivariable Cox regression analysis was performed to identify covariates associated with the 1-year mortality.The probability of survival was estimated using the Kaplan–Meier method,and curves were compared using thelog-rank test.Results:In total,688 patients were enrolled,with the median age was 88(84–91)years,and the majority(652,94.8%)were male.According to the KDIGO criteria,317 patients(46.1%)had Stage 1 AKI,169(24.6%)hadStage 2 AKI,and 202(29.3%)had Stage 3 AKI.Of the 688 study subjects,61(8.9%)with a duration of AKIlasted 1–2 days,104(15.1%)with a duration of AKI lasted 3–4 days,140(20.3%)with a duration of AKI lasted5–7 days,and 383(55.7%)with a duration of AKI lasted>7 days.Within each stage,a longer duration of AKIwas slightly associated with a higher rate of 1-year mortality.However,within each of the duration categories,the stage of AKI was significantly associated with 1-year mortality.When considered separately in multivariateanalyses,both the duration of AKI(3–4 days:HR=3.184;95%CI:1.733–5.853;P7 days:HR=1.766;95%CI:1.017–3.065;P=0.043)and more advanced AKIstage(Stage 2:HR=3.063;95%CI:2.207–4.252;P<0.001;Stage 3:HR=7.333;95%CI:5.274–10.197;P<0.001)were independently associated with an increased risk of 1-year mortality.Conclusions:In very elderly AKI patients,both a higher stage and duration were independently associated withan increased risk of 1-year mortality.H

关键词： Acute kidney injury Duration Very elderly 1-Year mortality

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某岛礁医院放射医疗工作的实践与启示

海军医学杂志 2023年第4期44卷 328-331页

作者：张叶顾群卞承玲闫飞虎海军军医大学第一附属医院影像科上海200433 海军军医大学第一附属医院肛肠外科上海200433 海军军医大学第一附属医院海军特色医学中心上海200433

目的总结某岛礁医院放射医疗工作的主要内容及方法,为同类型岛礁医院放射医疗工作开展提供借鉴和启示。方法回顾性分析某岛礁医院2016年10月初次接诊患者至2020年11月期间的全部放射医疗工作开展情况,统计分析驻礁官兵及相关海域作业人... 详细信息

目的总结某岛礁医院放射医疗工作的主要内容及方法,为同类型岛礁医院放射医疗工作开展提供借鉴和启示。方法回顾性分析某岛礁医院2016年10月初次接诊患者至2020年11月期间的全部放射医疗工作开展情况,统计分析驻礁官兵及相关海域作业人员常见X线检查部位、检查项目分布及对应检查结果的阳性率。结果共计完成2692人次的X线检查,其中胸部(体检)X线检查占比28.1%(756/2692),下肢X线检查占比22.6%(608/2692),上肢X线检查占比20.5%(552/2692),脊柱占比12.1%(327/2692),胸部占比8.2%(221/2692),上述5类检查部位占检查总量的91.5%。结论驻礁官兵及相关海域作业人员外伤以四肢伤居多,训练及作业过程需加强相应的防护。放射科人员、医疗设备科学配置,人工智能、远程医疗等创新应用,是岛礁医院今后开展放射医疗工作的努力方向。

关键词：岛礁医院放射摄影数字化X线摄影