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Aberrant DNA methyltransferase 1 expression in clear cell renal cell carcinoma development and progression

Chinese Journal of Cancer Research 2014年第4期26卷 371-381页

作者： Ming Li Ying Wang Yongsheng Song Renge Bu Bo Yin Xiang fei Qizhen Guo Bin wu Department of Urology Shengjing Hospital of China Medical University Department of Cell Biology Harvard Medical School Department of Nuclear Medicine The First Affiliated Hospital of China Medical University

Objective： To better understand the contribution of dysregulated DNA methyltransferase 1 （DNMT1） expression to the progression and biology of clear cell renal cell carcinoma （ccRCC）. Methods： We examined the differences in the expression of DNMT1 in 89 ecRCC and 22 normal tissue samples by immunohistochemistry. In addition, changes in cell viability, apoptosis, colony formation and invading ability of ccRCC cell lines （786-0 and Caki-1） were assessed after transfection with DNMT1 siRNA. Results： We found DNMT1 protein was significantly higher expressed in ccRCC than that of in no-tumor tissues （56.2% and 27.3%, respectively, P=0.018）. The expression of DNMT1 was strongly associated with ccRCC tumor size, tumor pathology stage, histological grading, lymph node metastasis, vascular invasion, recurrence and prognosis. Moreover, knockdown of DNMT1 expression significantly inhibited ccRCC cell viability, induced apoptosis, decreased colony formation and invading ability. Conclusions： Expression of DNMTI protein is increased in ccRCC tissues, and DNMT1 expression is associated with poor prognosis of patients. Experiments in vitro further showed DNMT1 played an essential role in proliferation and invasion of renal cancer cells. Moreover, targeting this enzyme could be a promising strategy for treating ccRCC, as evidenced by inhibited cell viability, increased apoptosis, decreased colony formation and invading ability.

关键词： Clear cell renal cell carcinoma (ccRCC) DNA methyltransferase I (DNMT1) immunohistochemistry siRNA

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Effects of freeze-thaw cycle on engineering properties of loess used as road fills in seasonally frozen ground regions,North China

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Journal of Mountain Science 2017年第2期14卷 356-368页

作者： LI Guo-yu MA Wei MU Yan-hu WANG fei FAN Shan-zhi wu Ya-hu State Key Laboratory of Frozen Soil Engineering Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of SciencesLanzhou 730000China

Compacted loess is widely used as fills of road embankments in loess regions of northern ***, densely-compacted loess can satisfy the requirements of embankment strength and postconstruction deformation. However, uneven subsidence, pavement cracks and other related damages can affect the integrity of loess subgrade after several years of operation,and even cause some hazards, especially in North China, where the strong freeze-thaw erosion occurs. In this study, cyclic freeze-thaw tests for both densely and loosely compacted loess samples were performed to determine the variation in engineering properties such as volume, void ratio, collapsible settlement,microstructure, and the related mechanisms were addressed. The experimental results showed that an obvious water migration and redistribution occurred within the samples during freeze-thaw cycles. Ice lenses and fissures could be identified in the upper frozen layers of the samples. After freeze-thaw cycles,the dry densities of the upper layers of samples changed significantly due to strong freeze-thaw erosion. The dry densities decreased for the dense sample and increased for the loose sample. It can be found that dense samples become loose, while loose samples became dense with the increasing number of freeze-thaw cycles. Their related void ratios changed reversely. Both void ratios tended to fall into a certain range, which verified the concept of a residual void ratio proposed by Viklander. The loosening process of densely compacted samples involves the formation of large pores, volume increase and density reduction as well as the related changes in mechanical properties because freeze-thaw cycles may be important contribution to problems of loess road *** effects of freeze-thaw cycles, therefore,should be taken into account in selecting loess parameters for the stability evaluation of road embankment in seasonally frozen ground regions.

关键词： Loess Freeze-thaw cycle Frost heave Thaw settlement Road engineering

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High yield synthesis of cyclic analogues of antibacterial peptides P-113 by Sortase A-mediated ligation and their conformation studies

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Chinese Chemical Letters 2017年第3期28卷 553-557页

作者： Zhi-Meng wu Shao-Zhong Liu Xiao-Zhong Cheng Xin-Rui Zhao Hao-fei Hong The Key Laboratory of Carbohydrate Chemistry 8' Biotechnology Ministry of Education School of Biotechnology Jiangnan University Wuxi 214122 China

P-113 is a fragment of natural occurring peptide Histatin 5 found in human saliva. This peptide exhibited broad spectrum of antibacterial and antifungal biological activities. In this study, bifunctional P-113 peptides 2–5 were designed as Sortase A substrates and synthesized by solid support peptide synthesis,where the N-terminus were equipped with glycine and its analogues, and C-terminus were extended with LPETGGS, respectively. Under Sortase A catalyzed condition, head to tail cyclization products 7–10were afforded in yields from 76% to 93%. The conformation insights of linear peptides 2–5 and cyclic analogues 7–10 in aqueous buffers and in trifluroethanol（TFE） analyzed by circular dichroism（CD）suggested that a-helix structures were produced progressively in hydrophobic environment independent of the cyclization, which displayed the similar behavior as parent peptide P-113.

关键词： Antibacterial peptide P-113 Cyclization Sortase A-mediated ligation Conformation Circular dichroism

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Forward Phase Conjugation Wave due to Multi-wave Coupling and Grating Share in Lithium Niobate Crystal

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Chinese Physics Letters 2001年第12期18卷 1601-1603页

作者： QIAO Hai-Jun XU Jing-Jun ZHANG Xin-Zheng LI fei-fei SUN Qian wu Qiang ZHANG Guang-Yin Photonics Research Center Department of PhysicsNankai UniversityTianjin 300071TEDA CollegeNankai UniversityTianjin 300457

The steady intensity of a forward phase conjugate wave (FPCW) is successfully observed along the c-axis in the highly magnesium-doped lithium niobate crystal. We put forward a detailed physical picture for our experiment phenomena. The FPCW is generated from the phase-mismatch in a three-wave coupling process, and tends to be stable accompanied with the building of photorefractive gratings and four-wave coupling processes. Our experimental result is also discussed and analysed by means of the wave coupling theory, and the theoretical expression is provided for the experiment.

关键词： wave Crystal coupling

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Surrogacy of bird species in systematic conservation planning and conservation assessments in Yunnan Province,China

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Journal of Mountain Science 2022年第10期19卷 2861-2873页

作者： ZHOU Jian YANG fei-ling ZHONG Zi-jie ZHANG Ji LENG Xian YE Jin wu Rui-dong Conservation Biogeography Research Group Institute of International Rivers and Eco-securityYunnan UniversityKunming 650091China Yunnan Key Laboratory of International Rivers and Transboundary Eco-security Yunnan UniversityKunming 650091China

Bird diversity is often used as surrogates for other species diversity,but there is a limited understanding of the extent of this surrogate in identifying representative priority conservation areas(PCAs).We used Yunnan Province in China as a case to measure the surrogacy of birds in systematic conservation ***,we collected distribution records of mammals,amphibians,reptiles,and birds from public databases and literature,and applied systematic conservation planning methods to identify *** targeted four taxonomic groups at conservation targets of 17%,31%,and 50%of the total study area,and used the Jaccard similarity index to evaluate the surrogate extent of bird PCAs for the other three ***,we overlapped different portfolios of PCAs with six categories of protected areas(PAs)in Yunnan,calculated the protection rates,and evaluated the effect of sustainable-use PAs on improving the protection rate of strict Nature *** results showed that bird PCAs generally had the highest surrogacy for representing PCAs of the other three *** the 50%conservation target,the Jaccard indices between birds and the other taxa reached about ***,amphibian PCAs had a good surrogacy for conserving reptiles,with a Jaccard index of 0.30,0.44 and 0.51 at the 17%,31%and 50%conservation targets,*** existing PA system had quite low coverages for PCAs of these four taxa,and the average protection rates were 22.97%,19.78%and 16.52%at the three levels of conservation targets,*** sustainable-use PAs can greatly complement the strict Nature Reserves by achieving average increased PA coverages of 10.00%,9.20%and 7.90%at the three conservation *** findings provide a basis for surrogate-based conservation planning for areas with insufficient biodiversity information.

关键词： Biodiversity surrogates Priority conservation areas Protected areas Systematic conservation planning Threatened species Yunnan

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在一个磁通量子内dc Josephson电流的阶梯效应(Ⅱ) 大尺寸Josephson结

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物理学报 1984年第8期 1075-1083页

作者：张裕恒沈秀玲郑捷飞吴彦汪茂泉中国科学技术大学物理系中国科学院等离子体物理研究所 82级研究生 82届毕业生.现在中国科学院半导体研究所. 现在美国UC Berkeley做研究生

大尺寸的Josephson结放置于Q值足够高的谐振腔中,当其Josephson频率ω=2cVc/h激励起腔的第r个本征模谐振时,这个振荡将反馈辐照结,必须考虑反馈场本征模的空间部分在结上是不同的。理论上得到了和文献[2]不同的结果,给出了阶跃电流随磁... 详细信息

大尺寸的Josephson结放置于Q值足够高的谐振腔中,当其Josephson频率ω=2cVc/h激励起腔的第r个本征模谐振时,这个振荡将反馈辐照结,必须考虑反馈场本征模的空间部分在结上是不同的。理论上得到了和文献[2]不同的结果,给出了阶跃电流随磁场的变化,分析了腔的反馈场本征模和Josephson结的辐射电磁波空间匹配的条件,解释了为什么大尺寸的Josephson结不存在周期小于φ0的阶梯效应的物理原因。

关键词：阶梯效应物理原因 dc Josephson Josephson 磁通量子大尺寸

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Asymptomatic hepatobiliary cystadenoma of the hepatic caudate lobe: a case report

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The Journal of Biomedical Research 2013年第4期27卷 336-338页

作者： Zhengdong fei Yeting Zhou Aiqing Peng Bohua wu Manhong Sun Shifang wu Departments of Ultrasound Shuyang People's Hospital Departments of Hepatobiliary Surgery Shuyang People's Hospital Departments of Radiology Shuyang People's Hospital

Human hepatobiliary cystadenoma is a rare benign cystic tumor of the liver, and is extremely rare in the caudate lobe. We herein present a case of a 70-year-old male with a hepatobiliary cystadenoma originating from t... 详细信息

关键词： liver biliary tract cystadenoma MRI ultrasonography

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不同地区油茶内生真菌多样性及其抑菌活性

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生物灾害科学 2018年第4期41卷 261-267页

作者：陈言柳裴男才吴斐张扬郭春兰张林平李子林江西农业大学鄱阳湖流域森林生态系统保护与修复国家林业局重点实验室江西南昌330045 中国林业科学研究院热带林业研究所广东广州510520 九连山国家级自然保护区管理局江西龙南341702

以江西、湖南、福建3个地区的油茶不同组织为材料,通过组织分离法分离其内生真菌并进行多样性分析和抑菌性分析。结果表明,共分离出352株内生真菌,隶属34属56种,其中子囊菌46种(86.65%),担子菌10种(13.35%)。球座菌属(Guigardia)、拟茎... 详细信息

以江西、湖南、福建3个地区的油茶不同组织为材料,通过组织分离法分离其内生真菌并进行多样性分析和抑菌性分析。结果表明,共分离出352株内生真菌,隶属34属56种,其中子囊菌46种(86.65%),担子菌10种(13.35%)。球座菌属(Guigardia)、拟茎点属(Phomopsis)、炭疽菌属(Colletotrichum)以及链格孢属(Alternaria)为优势属,分别占总株数的17.33%、16.19%、13.35%以及6.25%。从分离部位上看,叶片中的内生真菌分离率和定殖率最高,分别为71.11%和73.89%,而树皮中的内生真菌拥有最高的多样性指数;从采样地点上看,江西油茶内生真菌的分离率和定殖率最高,其次是湖南和福建。抑菌性结果显示,共有26种(46.43%)内生真菌具有抑菌活性,其中3株内生真菌(CF108,CF100,CF65)对所选的病原菌具有较强的抑制活性,表明这3株内生真菌具有较好的应用潜能。

关键词：油茶内生真菌多样性抑菌活性

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慢病毒介导酪氨酸激酶受体A过表达载体促进神经干细胞增殖和分化的实验研究

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安徽医药 2020年第12期24卷 2410-2414,I0003页

作者：邓明谢萍马永刚周炎贺斌陈庆吴飞陈忠辉明江华武汉大学人民医院骨科湖北武汉430060 武汉市第三医院中医科湖北武汉430060

目的探究酪氨酸激酶受体A(Trk A)过表达载体对神经干细胞增殖和分化能力的影响。方法取SD大鼠海马组织进行提取神经干细胞,采用免疫荧光的方法对神经球进行鉴定,利用siRNA技术构建Trk A过表达载体,将培养的神经干细胞分成三组,空白对照... 详细信息

目的探究酪氨酸激酶受体A(Trk A)过表达载体对神经干细胞增殖和分化能力的影响。方法取SD大鼠海马组织进行提取神经干细胞,采用免疫荧光的方法对神经球进行鉴定,利用siRNA技术构建Trk A过表达载体,将培养的神经干细胞分成三组,空白对照组为未做任何处理的细胞组,阴性对照组是经空白载体的慢病毒转染的细胞组,实验组是由Trk A的过表达载体构建的慢病毒转染细胞组。逆转录PCR(RT⁃PCR)检测Trk A和神经干细胞分化标志基因Tuj1,GFAP和CNPase的表达,CCK⁃8试剂盒检测各组细胞的增殖率。结果免疫荧光染色显示神经干细胞细胞球Nestin(红色荧光),Brud染色(绿色荧光)阳性,且占细胞量的90%以上;荧光双标染色(棕色荧光)显示双染细胞量占总细胞量的90%以上,说明培养的细胞是神经干细胞。慢病毒滴度的检测结果:Trk A过表达慢病毒载体转染滴度为3×10^8 TU/mL。RT⁃PCR结果表明,空白对照组(1.09±0.09)和阴性对照组(1.11±0.05)的Trk A的mRNA相对表达量比较差异无统计学意义(P>0.05),实验组的Trk A的mRNA相对表达量(7.12±1.32)要明显高于空白对照组和阴性对照组(P0.05),实验组的细胞增殖率要明显高于空白对照组和阴性对照组(P<0.05)。结论Trk A过表达载体可以促进神经元干细胞向神经元细胞,星型胶质细胞和少突胶质细胞的分化,并且可以促进神经元干细胞的增殖。

关键词：受体,Eph家族神经干细胞慢病毒属转染增殖分化大鼠,Sprague⁃Dawley

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Recent Advances and Opportunities of Lead-Free Perovskite Nanocrystal for Optoelectronic Application

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Energy Material Advances 2021年第1期2021卷 295-332页

作者： fei Zhang Zhuangzhuang Ma Zhifeng Shi Xu Chen Di wu Xinjian Li Chongxin Shan Key Laboratory of Materials Physics of Ministry of Education School of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China

Metal halide perovskite nanocrystals(NCs),as a new class of light-emitting and light-harvesting materials,have recently attracted intensive attention for an impressive variety of optoelectronic ***,the lead toxicity and poor stability of such materials severely restrict their practical applications and future ***-free perovskite NCs and their derivatives,designed by the reasonable chemical substitution of Pb with other nontoxic elements,are recently booming as an attractive alternative to lead-based *** this review,we firstly present a comprehensive overview of currently explored lead-free perovskite NCs with an emphasis on their design routes,morphologies,optoelectronic properties,and environmental stability ***,we discuss the preliminary achievements of lead-free perovskite NCs in versatile optoelectronic applications,such as light-emitting devices,solar cells,photodetectors,and *** finish this review with a critical outlook into the currently existing challenges and possible development opportunities of this rapidly evolving field.

关键词： field. perovskite stability

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