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The genomes of Taxus species unveil novel candidates in the biosynthesis of taxoids

Molecular Plant 2021年第11期14卷 1773-1775页

作者： Youjun Zhang federico scossa Alisdair R.Fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-lnstitut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Research Center for Genomics and Bioinformatics(CREA-GB) Via Ardeatina 54600178 RomeItaly

Taxaceae is the yew family of conifers and includes evergreen trees and small shrubs mainly distributed in Eurasia and North *** genus of Taxus has attracted considerable attention since Monroe *** and Mansukh *** firstly isolated and characterized an unusual cyclic diterpenoid from the bark of the Pacific yew tree(Wani et al.,1971).This compound,subsequently called paclitaxel,was shown to promote tubulin assembly into microtubules,preventing their ***,paclitaxel was approved for medical use against ovarian cancer in 1992(Fischer and Ganellin,2010).

关键词： unusual Monro compound

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Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis

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Molecular Plant 2023年第12期16卷 1951-1961页

作者： Youjun Zhang Lorenz Wiese Hao Fang Saleh Alseekh Leonardo Perez de Souza federico scossa John Molloy Mathias Christmann Alisdair R.Fernie Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Institute of Chemistry and Biochemistry Freie Universitat BerlinTakustraBe 314195 BerlinGermany Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces14476 PotsdamGermany Research Center for Genomics and Bioinformatics(CREA-GB) Via Ardeatina 54600178 RomeItaly

The diterpenoid paclitaxel(Taxol)is a chemotherapy medication widely used as a first-line treatment against several types of solid *** supply of paclitaxel from natural sources is ***,missing knowledge about the genes involved in several specific metabolic steps of paclitaxel biosynthesis has rendered it difficult to engineer the full *** this study,we used a combination of transcriptomics,cell biology,metabolomics,and pathway reconstitution to identify the complete gene set required for the heterologous production of *** identified the missing steps from the current model of paclitaxel biosynthesis and confirmed the activity of most of the missing enzymes via heterologous expression in Nicotiana ***,we identified a new C4β-C20 epoxidase that could overcome the first bottleneck of metabolic *** used both previously characterized and newly identified oxomutases/epoxidases,taxane 1β-hydroxylase,taxane 9aα-hydroxylase,taxane 9α-dioxygenase,and phenylalanine-CoA ligase,to successfully biosynthesize the key intermediate baccatin Ill and to convert baccatin Ill into paclitaxel in *** combination,these approaches establisha metabolic route to taxoidbiosynthesis and provide insights into the unique chemistry that plants use to generate complex bioactive metabolites.

关键词： paclitaxel biosynthesis synthetic biology baccatin Ill biosynthesis Taxus

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Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

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Horticulture Research 2020年第1期7卷 1149-1160页

作者： Evangelos Karagiannis Michail Michailidis Georgia Tanou federico scossa Eirini Sarrou George Stamatakis Martina Samiotaki Stefan Martens Alisdair R.Fernie Athanassios Molassiotis Laboratory of Pomology Department of AgricultureAristotle University of Thessaloniki54124 ThessalonikiGreece Institute of Soil and Water Resources ELGO-DEMETERThermiThessaloniki 57001Greece Max-Planck-Institute of Molecular Plant Physiology Am Müehlenberg 1.Potsdam-Golm 14476Germany Council for Agricultural Research and Economics Research Center for Genomics and BioinformaticsVia Ardeatina 54600178 RomeItaly Institute of Plant Breeding and Genetic Resources ELGO-DEMETERThermiThessaloniki 57001Greece Biomedical Sciences Research Center“Alexander Fleming” Vari 16672Greece Fondazione Edmund Mach Centro Ricerca e InnovazioneDepartment of Food Quality and NutritionVia E.Mach138010 San Michele all’AdigeTNItaly

Apple(Malus domestica Borkh)is an important fruit crop cultivated in a broad range of environmental *** fruit ripening is a physiological process,whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel *** this study,the influence of environmental conditions associated with low(20 m)and high(750 m)altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and *** apple fruit ripening was itself not affected by the different environmental conditions,several key color parameters,such as redness and color index,were notably induced by high *** with this observation,increased levels of anthocyanin and other phenolic compounds,including cyanidin-3-O-galactoside,quercetin-3-O-rhamnoside,quercetin-3-O-rutinoside,and chlorogenic acid were identified in the peel of apple grown at high ***,the high-altitude environment was characterized by elevated abundance of various carbohydrates(e.g.,arabinose,xylose,and sucrose)but decreased levels of glutamic acid and several related proteins,such as glycine hydroxymethyltransferase and glutamate–glyoxylate *** processes affected by high altitude were the TCA cycle,the synthesis of oxidative/defense enzymes,and the accumulation of photosynthetic *** the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel *** combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions.

关键词： altitude network prevailing

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Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex

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Molecular Plant 2020年第7期13卷 1027-1046页

作者： Takayuki Tohge federico scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie Max-Planck-lnstitut fiir Molekulare Pflanzenphysiologie Am Muehlenberg 114476 Potsdam-Golm.Germany Graduate School of Biological Science Nara Institute of Science and TechnologyIkoma 630-0192 Japan Council for Agricultural Research and Economics(CREA) Research Centre for Genomics and Bioinformaticsvia Ardeatina 54600178 RomeItaly Universite de Toulouse INP-ENSA ToulouseGenomique et Biotechnologie des FruitsCastanet-Tolosan 31326France Institute of Plant Systems Biology 4000 PlovdivBulgaria Max Planck Institute for Biology of Ageing Joseph Stelzmann Strasse 9b50931 CologneGermany Institute of Botany and Molecular Genetics BioSCRWTH Aachen University52056 AachenGermany National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China

While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across *** this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of *** this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic *** combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid *** combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit *** results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.

关键词： Solanumly copersicum secondary metabolism natural diversity wild accessions pathway elucidation gene discovery

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