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Stable diffusion gradients in microfluidic conduits bounded by fluid walls

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Microsystems & Nanoengineering 2024年第3期10卷 357-367页

作者： federico Nebuloni Cyril Deroy Peter R.Cook Edmond j.Walsh Department of Engineering Science Osney Thermo-Fluids InstituteUniversity of OxfordOxford OX20ESUK Sir William Dunn School of Pathology University of OxfordOxford OX13REUK

Assays mimicking in vitro the concentration gradients triggering biological responses like those involved in fighting infections and blood clotting are essential for biomedical *** assays prove especially attractive as they allow precise control of gradient shape allied to a reduction in *** microfluidic devices are fabricated using solid plastics that prevent direct access to responding ***-walled microfluidics allows the manufacture of circuits on standard Petri dishes in seconds,coupled to simple operating methods;cell-culture medium sitting in a standard dish is confined to circuits by fluid walls made of an immiscible *** develop and experimentally validate an analytical model of diffusion between two or more aqueous streams flowing at different rates into a fluid-walled conduit with the cross-section of a circular *** solid walls,fluid walls morph during flows as pressures fall,with wall shape changing down the *** model is validated experimentally for Fourier numbers<0.1 using fluorescein diffusing between laminar *** enables a priori prediction of concentration gradients throughout a conduit,so allowing rapid circuit design as well as providing bioscientists with an accurate way of predicting local concentrations of bioactive molecules around responsive and nonresponsive cells.

关键词： walls diffusion attractive

Distinct vibrational signatures and complex phase behavior in metallic oxygen

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Matter and Radiation at Extremes 2024年第2期9卷 4-11页

作者： Philip Dalladay-Simpson Bartomeu Monserrat Li Zhang federico Gorelli Center for High Pressure Science Technology Advanced Research 1690 Cailun RoadShanghai 201203China Department of Materials Science and Metallurgy University of Cambridge27 Charles Babbage RoadCambridge CB3 OFSUnited Kingdom Cavendish Laboratory University of CambridgeJ.J.Thomson AvenueCambridge CB3 OHEUnited Kingdom Istituto Nazionale di Ottica Consiglio Nazionale delle Ricerche(CNR-INO)50125 FlorenceItaly Shanghai Advanced Research in Physical Sciences(SHARPS) ShanghaiChina

Evidence for metallization in dense oxygen has been reported for over 30 years[Desgreniers et al.,***.94,1117(1990)]at a now routinely accessible 95 GPa[Shimizu et al.,Nature 393,767(1998)].However,despite the longevity of this result and the technological advances since,the nature of the metallic phase remains poorly constrained[Akahama et al.,***.74,4690(1995);Goncharov et al.,***.B 68,224108(2003);Ma,***.B 76,064101(2007);and Weck et al.,***.102,255503(2009)].In this work,through Raman spectroscopy,we report the distinct vibrational characteristics of metallicζ-O_(2) from 85 to 225 *** comparison with numerical simulations,weﬁnd reasonable agreement with the candidate structure up to about 150 *** higher pressures,the C2/mstructure is found to be unstable and incompatible with experimental *** candidate structures,and Ci,with C2/m C2/conly two molecules in the primitive unit cell,are found to be stable and more compatible with measurements above 175 GPa,indicative of the dissociation of(O_(2))4 ***,we report and discuss a strong hysteresis and metastability with the precursory phaseϵ-O_(2).Theseﬁndings will reinvigorate experimental and theoretical work into the dense oxygen system,which will have importance for oxygen-bearing chemistry,prevalent in the deep Earth,as well as fundamental physics.

关键词： metallic phase vibrational

CONSTANS alters the circadian clock in Arabidopsis thaliana

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Molecular Plant 2024年第8期17卷 1204-1220页

作者： Pedro de los Reyes Gloria Serrano-Bueno Francisco j.Romero-Campero He Gao jose M.Romero federico Valverde Plant Development Group-Institute for Plant Biochemistry and Photosynthesis Consejo Superior de Investigaciones CientificasUniversidad de SevillaSevilleSpain Department of Computer Science and Artificial Intelligence Universidad de SevillaSevilleSpain Department of Plant Developmental Biology Max Planck Institute for Plant Breeding ResearchCologneGermany Department of Plant Biochemistry and Molecular Biology Universidad de SevillaSevilleSpain

Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the *** these processes,the floral transition is essential to ensure reproductive success and is finelyregulated by several internal andexternal genetic *** photoperiodic pathway,which controls plant response to day length,is one of the most important pathways controlling *** Arabidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long *** circadian clock strongly regulates Co ***,to date,no evidence has been reported regarding a feedbackloop from the photoperiod pathway back to the circadian *** transcriptional networks,we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod *** expression,chromatin immunoprecipitation experiments,and phenotypic analysis allowed us to elucidate the role of Co over the circadian *** with altered cO expression showed a different internal clock period,measured by daily leaf rhythmic *** showed that co upregulates the expression of key genes related to the circadian clock,such as CCA1,LHY,PRR5,and Gl,at the end of a long day by bindingto specific sites on their ***,a high numberof PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition promoted by *** CO-PRR5 complex interacts with the bZiP transcriptionfactor HY5andhelps to localize the complex in the promoters of clock *** together,our results indicate that there may be a feedback loop in which co communicates back to the circadian clock,providing seasonal information to the circadian system.

关键词： CONSTANS circadian clock photoperiod

Low-Cost and Biodegradable Thermoelectric Devices Based on van der Waals Semiconductors on Paper Substrates

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Energy & Environmental Materials 2024年第1期7卷 205-210页

作者： Gulsum Ersu Carmen Munuera federico j.Mompean Daniel Vaquero jorge Quereda jo?o Elias F.S.Rodrigues jose A.Alonso Eduardo Flores jose R.Ares Isabel j.Ferrer Abdullah M.Al-Enizi Ayman Nafady Sruthi Kuriakose joshua O.Island Andres Castellanos-Gomez Materials Science Factory Instituto de Ciencia de Materiales de Madrid (ICMM-CSIC) Nanotechnology Group USAL–Nanolab Univesidad de Salamanca GISC Departamento de Física de Materiales Universidad Complutense de Madrid Departamento de Física Aplicada Centro de Investigación y Estudios Avanzados MIRE Group Departamento de Física de Materiales Universidad Autónoma de Madrid Instituto Nicolás Cabrera Universidad Autónoma de Madrid Department of Chemistry College of Science King Saud University Department of Physics and Astronomy University of Nevada Las Vegas

We present a method to fabricate handcrafted thermoelectric devices on standard office paper *** devices are based on thin films of WS2,Te,and BP（P-type semiconductors） and TiS3and TiS2（N-type semiconductors）,depo... 详细信息

关键词： paper-based electronics Seebeck effect semiconductors thermoelectrics van der Waals materials

Oxidized organic molecules in the tropical free troposphere over Amazonia

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National Science Review 2024年第1期11卷 174-184页

作者： Qiaozhi Zha Diego Aliaga Radovan Krejci Victoria A.Sinclair Cheng Wu Giancarlo Ciarelli Wiebke Scholz Liine Heikkinen Eva Partoll Yvette Gramlich Wei Huang Markus Leiminger joonas Enroth Otso Per?kyl? Runlong Cai Xuemeng Chen Alkuin Maximilian Koenig Fernando Velarde Isabel Moreno Tuukka Pet?j? Paulo Artaxo Paolo Laj Armin Hansel Samara Carbone Markku Kulmala Marcos Andrade Douglas Worsnop Claudia Mohr federico Bianchi Institute for Atmospheric and Earth System Research/Physics University of Helsinki joint International Research Laboratory of Atmospheric and Earth System Sciences Nanjing University Department of Environmental Science & Bolin Centre for Climate Research Stockholm University Department of Chemistry and Molecular Biology University of Gothenburg Institute for Ion and Applied Physics University of Innsbruck Ionicon Analytik GmbH Laboratory for Atmospheric Physics Institute for Physics Research Universidad Mayor de San Andrés Institute of Physics University of Sao Paulo Institute for Geosciences and Environmental Research (IGE) University of Grenoble Alpes Agrarian Sciences Institute Federal University of Uberl?ndia Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Department of Atmospheric and Oceanic Sciences University of Maryland Aerodyne Research Inc. Department of Environmental System Science ETH Zürich Switzerland and Laboratory of Atmospheric Chemistry Paul Scherrer Institute

New particle formation(NPF) in the tropical free troposphere(FT) is a globally important source of cloud condensation nuclei, affecting cloud properties and climate. Oxidized organic molecules(OOMs)produced from biogenic volatile organic compounds are believed to contribute to aerosol formation in the tropical FT, but without direct chemical observations. We performed in situ molecular-level OOMs measurements at the Bolivian station Chacaltaya at 5240 m above sea level, on the western edge of Amazonia. For the first time, we demonstrate the presence of OOMs, mainly with 4–5 carbon atoms, in both gas-phase and particle-phase(in terms of mass contribution) measurements in tropical FT air from Amazonia. These observations, combined with air mass history analyses, indicate that the observed OOMs are linked to isoprene emitted from the rainforests hundreds of kilometers away. Based on particle-phase measurements, we find that these compounds can contribute to NPF, at least the growth of newly formed nanoparticles, in the tropical FT on a continental scale. Thus, our study is a fundamental and significant step in understanding the aerosol formation process in the tropical FT.

关键词： oxidized organic molecules tropical aerosol formation Amazon free troposphere

Shedding Light on Large-Scale Chromatin Reorganization in Arabidopsis thaliana

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Molecular Plant 2012年第3期5卷 583-590页

作者： Martijn van Zanten federico Tessadori Anton j.M. Peeters Paul Fransz Molecular Plant Physiology Institute of Environmental Biology Utrecht University Padualaan 8 3584 CH Utrecht The Netherlands Department of Plant Breeding and Genetics Max Planck Institute for Plant Breeding Research Carl-von-Linne＇-weg 10 50829 Cologne Germany Cardiac Development and Genetics Group Hubrecht Institute for Developmental Biology and Stem Cell Research Uppsalalaan 8 3584 CT Utrecht The Netherlands Plant Ecophysiology Institute of Environmental Biology Utrecht University Padualaan 8 3584 CH Utrecht The Netherlands Nuclear Organization Group Swammerdam Institute for Life Sciences University of Amsterdam Kruislaan 318 1098 SM Amsterdam The Netherlands

Plants need to respond quickly and appropriately to various types of light signals from the environment to optimize growth and development. The immediate response to shading, reduced photon flux （low light）, and changes in spectral quality involves changes in gene regulation. In the case of more persistent shade, the plant shows a dramatic change in the organization of chromatin. Both plant responses are controlled via photoreceptor signaling proteins. Re- cently, several studies have revealed similar features of chromatin reorganization in response to various abiotic and biotic signals, while others have unveiled intricate molecular networks of light signaling towards gene regulation. This opinion paper briefly describes the chromatin （de）compaction response from a light-signaling perspective to provide a link be- tween chromatin and the molecular network of photoreceptors and E3 ubiquitin ligase complexes.

关键词： light signaling chromatin compaction molecular network Arabidopsis

Mobile NMR for geophysical analysis and materials testing

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Petroleum Science 2009年第1期6卷 1-7页

作者： BLüMICH Bernhard MAULER jrg HABER Agnes PERLO juan DANIELI Ernesto CASANOVA federico Institut für Technische und Makromolekulare Chemie RWTH Aachen University

Initiated by well logging NMR, portable NMR instruments are being developed for a variety of novel applications in materials testing, process analysis and control, which provides new opportunities for geophysical investigations. Small-diameter cylindrical sensors can probe short distances into the walls of slim-line logging holes, and single-sided sensors enable non-destructive testing of large objects. Both sensors are characterized by small sensitive volumes. Barrel-shaped magnets that accommodate the sample in their center have higher sensitivity due to a larger sensitive volume but can accommodate only samples like drill cores, which fit in size to the diameter of the magnet bore. Both types of magnets can be scaled down to the size of a coffee mug to arrive at sub-compact NMR equipment. Portable NMR magnets are reviewed in the context of applications related to geophysics.

关键词： Mobile NMR portable NMR relaxation imaging spectroscopy

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Biomechanical analysis of the "running" vs. "conventional" diagonal stride uphill techniques as performed by elite cross-country skiers

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journal of Sport and Health Science 2022年第1期11卷 30-39页

作者： Barbara Pellegrini Chiara Zoppirolli federico Stella Lorenzo Bortolan Hans-Christer Holmberg federico Schena CeRiSM(Research Centre of Mountain Sport and Health) University of VeronaRovereto 38068Italy Department of Neuroscience Biomedicine and MovementUniversity of VeronaVerona 37129Italy Swedish Winter Sports Research Centre Department of Health SciencesMid Sweden UniversityÖstersund 83125Sweden School of Kinesiology University of British ColumbiaVancouver BC V6T 1Z1Canada Biomechanics Laboratory Beijing Sport UniversityBeijing 100084China

Purpose:This study aimed to compare biomechanical aspects of a novel"running"diagonal stride(DSRUN)with"conventional"diagonal stride(DSCONV)skiing techniques performed at high ***:Ten elite Italian male junior cross-country skiers skied on a treadmill at 10 km/h and at a 10°incline utilizing both variants of the diagonal stride *** 3-dimensional kinematics of the body,poles,and roller skis;the force exerted through the poles and foot plantar surfaces;and the angular motion of the leg joints were ***:Compared to DSCONV,DSRUN demonstrated shorter cycle times(1.05±0.05 s vs.0.75±0.03 s(mean±SD),pj/m/kg vs.0.10±0.02 j/m/kg,pjoint extension than DSCONV-Further investigations,preferably on snow,should reveal whether DSRUN results in higher acceleration and/or higher peak speed.

关键词： Classical skiing Kinetics Roller skiing

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The case of the missing appendix:a case report of appendiceal intussusception at the site of colonic mullerianosis

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Gastroenterology Report 2017年第4期5卷 309-312,I0003页

作者： federico P Quirante Lisandro M Montorfano federico Serrot Mary E Billington Giovanna Da Silva Emanuele Lo Menzo Samuel Szomstein Raul j Rosenthal Department of Surgery Cleveland Clinic FloridaWestonFLUSA

Right lower quadrant pain is a symptom with an exceptionally broad differential *** of the appendix is a very uncommon condition with many ***,the pathologic finding of ectopic presence of a mixture of at least two mullerian-derived tissue components is *** report presents the case of a 49-year-old woman who presented twice with acute right lower abdominal *** of appendiceal inversion was made *** examination of the specimen identified extensive endometriosis,endosalpingiosis and endocervicosis of the colon *** intussusception and colonic mullerianosis,present together,are discussed,and recommendations for the diagnosis and treatment of appendiceal intussusception are discussed.

关键词： appendix appendiceal intussusception mullerianosis endocervicosis