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Advances in Alzheimer's Disease 2017年第1期6卷 13-31页

作者： Teresa Ponce-Lopez enrique Hong Manuel abascal-Díaz Alfredo Meneses Deparment of Pharmacobiology CINVESTAV México City México Faculty of Health Sciences Anahuac University Huixquilucan State of México Mexico

Intracerebroventricular administration (ICV) of streptozotocin (STZ) in rats has been associated to desensitization of the insulin receptor (IR) and biochemical changes similar to those occurring in Alzheimer’s disease (AD) or older brains, so it has been proposed as a suitable model for studying some of the pathological features of AD sporadic type (SAD). In this study, we investigated the role of glycogen synthase kinase 3β (GSK3β) and protein phosphatase 2A (PP2A) in the regulation of the phosphorylation of tau (p-tau). Results showed that ICV-STZ treated rats had deficits in short- (1.5-h) and long-term (24- and 48-h) memory after one month of ICV-STZ treatment and six months relative to control rats. The memory deficit was associated to increasing [F(3, 12) = 31.48, p β (p-GSK3β) and PP2A in hippocampus and PFC, indicating that GSK3β and PP2A contributed to regulation of p-tau. These data supporting the model with ICV-STZ in rat are adequate to study the progressive memory impairment associated to hyperphosphorylation of tau and the cascade of insulin receptor signaling;confirm that phosphatidyl-inositol-3 kinase-protein kinase B (PI3K-PKB/Akt-GSK3β) and PP2A are involved in the modulation of proteins responsible for the regulation of neurodegeneration in AD.

关键词： Memory Deficit Tau Hyperphosphorylation GSK3β PP2A Streptozotocin Hippocampus

SAP expression in Candida albicans Strains Isolated from Mexican Patients with Vaginal Candidosis

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International Journal of Clinical Medicine 2013年第1期4卷 25-31页

作者： eric Monroy-Pérez Gloria Paniagua-Contreras felipe Vaca-Paniagua erasmo Negrete-abascal Sergio Vaca feS-Iztacala Universidad Nacional Autónoma de MéxicoMéxicoDFMéxico Laboratorio de Genómica Instituto Nacional de CancerologíaMéxicoDFMéxico

To determine the frequency and expression of the ten SAP (secreted aspartyl protease) genes in a group of Candida albicans strains isolated from Mexican women suffering from vaginal candidosis, a group of 264 women (age 18 - 57 years) with vaginal infections, predisposed by diabetes mellitus or contraceptive consumption, were evaluated. C. albicans was identified using PCR to amplify the rRNA internal transcribed spacer regions ITS1 and ITS2. The presence of the SAP genes was determined using conventional PCR, and their expression levels were determined using real-time PCR after the C. albicans strains had been grown in reconstituted human vaginal epithelium (RHVe). C. albicans was identified in the samples from 50 women (18.9%). The genotyping frequencies of the SAP genes were as follows: SAP1, 94%;SAP2, 98%;SAP3, 80%;SAP4, 100%;SAP5, 100%;SAP6, 100%;SAP7, 63%;SAP8, 96%;SAP9, 70%;and SAP10, 88%. The most frequently expressed genes in the strains harboring all of the genes were SAP1, 90%;SAP2, 90%;SAP3, 90%;SAP4, 100%;SAP5, 90%;SAP6, 90%;SAP7, 100%;SAP8, 90%;SAP9, 100%;and SAP10, 100%. SAP genes were expressed in the RHVe, suggesting that the Sap proteins play an important role in the pathogenesis of infection.

关键词： Candida albicans RHVe SAP expression

Virulence Markers in Staphylococcus aureus Strains Isolated from Hemodialysis Catheters of Mexican Patients

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Advances in Microbiology 2012年第4期2卷 476-487页

作者： Gloria Paniagua-Contreras Teresita Sainz-espunes eric Monroy-Perez Jose Raymundo Rodriguez-Moctezuma Diego Arenas-Aranda erasmo Negrete-abascal Sergio Vaca feS-Iztacala Universidad Nacional Autonoma de Mexico(UNAM)TlalnepantlaMéxico Ciencias Biológicas y de la Salud Universidad Autonoma Metropolitana(UAM)—XochimilcoCalzada del HuesoMéxico Departamento de Sistemas Biológicos División de Ciencias Biológicas y de la SaludUniversidad Autonoma Metropolitana(UAM)—XochimilcoCalzada del HuesoMéxico Hospital General Regional 72 Instituto Mexicano del Seguro SocialGustavo Baz Esquina F.GómezTlalnepantlaMéxico

Methicillin-resistant Staphylococcus aureus is an increasingly important cause of nosocomial bacterial infections worldwide. S. aureus is responsible for several pathologies, including skin infections, endocarditis, meningitis, deep-tissue ulcers, and sepsis. S. aureus biofilm formation on catheters and other medical devices is a major post-operative concern, because biofilms are often the source of persistent and difficult to treat bacterial infections. While catheter-related S. aureus infections have been reported, the strains responsible for these infections have not been genetically characterized. We genetically characterized S. aureus strains isolated from hemodialysis catheters in Mexican patients. The frequency of 35 genes coding for adhesins, toxins, and other virulence-associated products in the 55 isolated S. aureus strains was determined using PCR, while real-time PCR was used to examine the level of gene expression. Of the 55 S. aureus strains isolated from 109 patients, 45 (81.8%) were determined to be methicillin-resistant. The icaA, rbf, sarA, and agr genes are involved in biofilm formation and bacterial dispersion and were detected in 96.3%, 40.0%, 74.5%, and 100% of S. aureus strains, respectively, and 70.9% of the strains formed a detectable biofilm. Interestingly, 67.2% of the strains contained the icaA, agr, spa, clfA, sdrC, sdrD, sdre, seg, seh, and sei genes, suggesting that this gene combination is important for successful catheter colonization. The results of this study provide significant insight into the virulence gene make-up of catheter-colonizing S. aureus strains, and will assist in developing a more targeted treatment approach for persistent S. aureus biofilm contamination of medical devices.

关键词： S.aureus Hemodialysis Catheter Genotyping

Prefrontal and Accumbal electric Activity during Auditory Stimulation in Virgin female Rats: Changes Related to the estrous Cycle

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Journal of Behavioral and Brain Science 2013年第6期3卷 454-462页

作者： Marisela Hernández-González Diego Radberto Chapa García abascal Miguel ángel Guevara Ricardo Alonso Romero Orozco Mayra Linné Almanza Sepúlveda Leopoldo eduardo Flores Mancilla Facultad de Medicina Universidad Autónoma de Zacatecas Unidad Académica de Medicina Humana y Ciencias de la Salud Zacatecas México Instituto de Neurociencias Universidad de Guadalajara Guadalajara México

The estrous cycle is a physiological process modulated by hormonal changes associated, in turn, with alterations of the attraction or sensitivity with which females perceive different sensory stimuli. Auditory stimuli play an important role in the social interaction of several mammals, including their sexual behavior and the mother-young relationship. Since the prefrontal cortex (PFC) and nucleus accumbens (Acc) had been associated with the processing and assignation of the incentive value of stimuli, this study was designed to analyze whether electroencephalographic (eeG) activity in the PFC and Acc was modified during the two antagonist phases of the estrous cycle (proestrus-estrus and diestrus) when female rats heard sound recordings with different types of cries emitted by other rats. eeGs were recorded in eight female virgin Wistar rats, 80-100 days old, bilaterally implanted in the PFC and Acc during an awake-quiet state, in three conditions: when hearing 1) cries of pups;2) cries of adult male rats;and, 3) cries of adult female rats. The cries were recorded from pups (2-10 days old) and male and female adult rats after gentle prodding by a human hand. each auditory stimulus (which included vocalizations up to 22 kHz) was randomly played back through a speaker placed near the female rats. Only upon perceiving the cries of male rats did the females in the proestrus-estrous phase show a lower relative power in the 14-30 Hz band in the Acc and an increased inter-prefrontal correlation in the 8-13 Hz band. It is probable that these eeG changes are associated with the lower activation or non-anxious state that the female rat presents specifically during the proestrus-estrous phase in response to the alarm cries of adult male rats.

关键词： Auditory Stimulation eeG Accumbens Prefrontal Cortex estrous Cycle Rats

Adhesion of <i>Gallibacterium anatis</i>to Chicken Oropharyngeal epithelial Cells and the Identification of Putative Fimbriae

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Advances in Microbiology 2012年第4期2卷 505-510页

作者： Mónica L. Salgado Lucio Sergio Vaca Candelario Vázquez edgar Zenteno Ismael Rea Víctor M. Pérez-Márquez erasmo Negrete-abascal Biotecnología Veterinaria Tehuacán Mexico Carrera de Biología Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México (UNAM) Tlalnepantla México Centro de Investigaciones en Ciencias Microbiológicas Benemérita Universidad Autónoma de Puebla (BUAP) Puebla México Departamento de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México México City México

Microbial infections are typically initiated by the colonization of tissues by a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized the ability of Gallibacterium anatis F149T to express fimbriae that may be involved in mucosal attachment. Using transmission electron microscopy, the fimbriae-like structures could be observed on the surface of negatively stained G. anatis F149T, and these structures were further visualized after being released by physical shaking. When the fimbriae-like structures were separated by SDS-PAGe, the proteins comprising them were isolated and sized at 13 and 25 kDa. G. anatis F149T was able to adhere to chicken oropharyngeal epithelial cells. Adhesion could be completely inhibited by pretreatment of the bacterial cells with trypsin, whereas 25% inhibition was attained after pretreatment with an antiserum against the 13 kDa protein. We demonstrated by immuno-gold electron microscopy that the antibodies from the antiserum were specifically associated with the fimbria-like structures on G. anatis. These results indicated that G. anatis F149T expresses fimbriae that contribute to its adhesion to chicken oropharyngeal epithelial cells and may be important for colonization of the upper respiratory tract.

关键词： G. anatis Adhesion Fimbria Pili

ethanol effects on Cortical eeG Correlation and Sexual Behavior in Male Rats

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Journal of Behavioral and Brain Science 2014年第2期4卷 92-98页

作者： Miguel Angel Guevara Marisela Hernández-González Mayra Linné Almanza-Sepúlveda Diego Radberto Chapa García abascal Pilar Durán Carolina Sotelo Tapia Fabricio Luna Torres Departamento de Biología Celular Facultad de Ciencias Universidad Nacional Autónoma de México México DF México Instituto de Neurociencias Universidad de Guadalajara Guadalajara Jalisco México

ethanol affects sexual behavior drastically, as much as its execution as well as its motivation. ethanol is a central nervous system depressant and the prefrontal cortex (PFC) is one of the cortical areas most sensitive to its effects. This prefrontal area participates in the modulation of the cognitive control, and furthermore, by mean of connections with the parietal cortex (PC) plays an important role in the processing of sexually relevant stimuli. Considering that it has been reported that the ethanol affects the proper functioning of these cortical areas, particularly decreasing the degree of electroencephalographic (eeG) coupling or correlation between them, the aim of this study was to characterize the effect of a low dose of ethanol on eeG correlation, as well determining if this effect is associated with impaired sexual behavior in male rats. Nine male rats unilaterally implanted were simultaneously recorded the eeG from the PFC and PC to calculate the degree of eeG synchronization (correlation, r) between both cortices during the first 40 minutes post administration of 0.75 g/kg of ethanol. At the end of the eeG recording, the males were submitted to copulatory behavior with a receptive female rat. The ethanol administration was associated with a decreased intrahemispheric correlation of the fast bands between PFC and PC, as well as with an increased mount and intromission latency. This data showed that ethanol in moderate doses decreased the coupling degree between cortices. It is likely that this functional disconnection between the PFC and PC after the ethanol administration could be associated with the difficulty in discriminating incentive value of the sexual stimuli so that decreased the sexual motivation but not the performance of the copulatory parameters in the male rat.

关键词： ethanol Sexual Behavior eeG Prefrontal Cortex Parietal Cortex Rat

Limited long-distance dispersal success in a Western european fairy shrimp evidenced by nuclear and mitochondrial lineage structuring

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Current Zoology 2020年第3期66卷 227-237页

作者： Paula C.RODRIGUeZ-FLOReS ernesto ReCUeRO Yolanda JIMeNeZ-RUIZ Mario GARCIA-PARIS Museo Nacional de Ciencias Naturales(MNCN-CSIC) Jose Gutierrez Abascal2Madrid 28006Spain Centre d'estudis Avanqats de Blanes(CeAB-CSIC) C.d'Acces Cala Sant Francesc 14Blanes 17300Spain

Anostraca are known by their ability for long-distance dispersal,but the existence in several species of deep,geographically structured mtDNA lineages suggests their populations are subjected to allopatric differentiation,isolation,and prevalence of local scale *** stagnalis is one of the most widespread species of Anostraca and previous studies revealed an unclear geographical pattern of mtDNA genetic ***,we analyze populations from the Iberian and Italian Peninsulas,Central europe,and Scandinavia,with the aim to characterize the patterns of genetic diversity in a spatio-temporal framework using mtDNA and nuclear markers to test gene flow among close *** these aims we built a time-calibrated phylogeny and carried out Bayesian phylogeographic analyses using a continuous diffusion *** results indicated that *** presents a deeply structured genetic diversity,including 7 ancient lineages,some of them even predating the *** Iberian Peninsula harbors high diversity of lineages,with strong isolation and recent absence of gene flow between *** at local scale seems to be the prevailing dispersal mode of ***,which exhibits a pattern of isolation-bydistance in the Iberian *** remark the vulnerability of most of these lineages,given the limited known geographic distribution of some of them,and the high risk of losing important evolutionary potential for the species.

关键词： allopatric fragmentation Anostraca Crustacea nuclear markers Pleistocene phylogeography

新研究确定了与肝病和脂肪代谢有关的肝细胞突变

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中西医结合肝病杂志 2021年第11期31卷 1011-1011页

作者： Stanley W. K. Ng Foad J. Rouhani Simon F. Brunner Natalia Brzozowska fe.e.ico abascal Luiza Moore Lia Chappell Daniel Leongamornlert Aleksandra Ivovic Philip Robinson Timothy Butler Mathijs A. Sanders Nicholas Williams Tim H. H. Coorens Jon Teague Keiran Raine Adam P. Butler Yvette Hooks Beverley Wilson Natalie Birtchnell Michael R. Stratton Iñigo Martincorena Raheleh Rahbari Peter J. Campbell Foad J. Rouhani & Huw Naylor Sarah J. Aitken & Matthew Hoare Sarah J. Aitken & Susan e. Davies Sarah J. Aitken Ming Yang efterpi Nikitopoulou Christian Frezza Matthew Hoare Cancer Genome Project Wellcome Sanger Institute Hinxton UK Department of Hematology Erasmus University Medical Center Rotterdam The Netherlands Stem Cell Institute University of Cambridge Cambridge UK Department of Surgery Addenbrooke’s Hospital Cambridge UK CRUK Cambridge Institute Cambridge UK Department of Pathology Addenbrooke’s Hospital Cambridge UK MRC Toxicology Unit University of Cambridge Cambridge UK MRC Cancer Unit University of Cambridge Cambridge UK Department of Medicine University of Cambridge Addenbrooke’s Hospital Cambridge UK

一项来自英国韦尔科姆基金会桑格研究所和剑桥大学等研究机构的研究人员的新研究分析了来自34例患者肝脏样本的1590个基因组,包括健康对照、酒精相关肝病和非酒精性脂肪肝疾病患者的肝脏样本,结果发现ACVR2A、TNRC6B、NeAT1、FOXO1和GP... 详细信息

一项来自英国韦尔科姆基金会桑格研究所和剑桥大学等研究机构的研究人员的新研究分析了来自34例患者肝脏样本的1590个基因组,包括健康对照、酒精相关肝病和非酒精性脂肪肝疾病患者的肝脏样本,结果发现ACVR2A、TNRC6B、NeAT1、FOXO1和GPAM 五个基因在肝病患者的肝细胞中发生了突变,过程如图a所示。而其中的FOXO1、CIDeB、GPAM三个基因是对肝细胞如何代谢脂肪和对胰岛素作出反应有直接影响的基因。

关键词： FOXO1 肝病患者细胞突变肝细胞脂肪代谢胰岛素 GPA 剑桥大学

Self-modulation and anomalous collective scattering of laser produced intense ion beam in plasmas

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Matter and Radiation at extremes 2018年第3期3卷 127-134页

作者： K.Mima J.Fuchs T.Taguchi J.Alvarez J.R.Marques S.N.Chen T.Tajima J.M.Perlado The Graduate School for the Creation of New Photonics Industries 1955-1KurematsuNishikuHamamatsuJapan LULI-CNRS Ecole PolytechniqueCEA:Universite Paris-SaclayUPMC Univ Paris 06:Sorbonne UniversitesF-91128Palaiseau CedexFrance Faculty of Science and engineering Setsunan UniversityNeyagawa572-8508OsakaJapan Instituto de Fusion Nuclear ETSI de IndustrialesUniversidad Politecnica de MadridC/Jose Gutierrez Abascal2E-28006MadridSpain Department of Physics and Astronomy University of California at IrvineIrvineCA92697USA

The collective interaction between intense ion beams and plasmas is studied by simulations and experiments,where an intense proton beam produced by a short pulse laser is injected into a pre-ionized *** is found that,depending on its current density,collective effects can significantly alter the propagated ion beam and the stopping *** quantitative agreement that is found between theories and experiments constitutes the first validation of the collective interaction *** effects in the interaction between intense ion beams and background gas plasmas are of importance for the design of laser fusion reactors as well as for beam physics.

关键词： Two stream instabilities Ultra intense short pulse laser Proton beam Wake field electron plasma wave Laser plasma interaction

GABA/孕酮激发豚鼠精子聚磷酸肌醇降解及其在顶体反应中的作用

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中国科学（C辑） 2001年第2期31卷 142-149页

作者：袁玉英毛丽珍石其贤陈文颖 e.R.S.Roldan 俞淑清庄元忠徐世芳浙江省医学科学院 National Museum of Natural Sciences(CSIC Jose Gutierrez Abascal 228006-Madrid Spain)

为了研究ＧＡＢＡ／孕酮（Ｐ４）激发精子聚磷酸肌醇（ＰＰＩ）降解及其在顶体反应（ＡＲ）中的作用，将豚鼠精子在ＭＣＭ培养基获能培养５．５ｈ，３２Ｐ－标记精子１ｈ，经Ｐｅｒｃｏｌｌ密度梯度离心洗涤，然后以ＡＲ刺激剂激发精子... 详细信息

为了研究ＧＡＢＡ／孕酮（Ｐ４）激发精子聚磷酸肌醇（ＰＰＩ）降解及其在顶体反应（ＡＲ）中的作用，将豚鼠精子在ＭＣＭ培养基获能培养５．５ｈ，３２Ｐ－标记精子１ｈ，经Ｐｅｒｃｏｌｌ密度梯度离心洗涤，然后以ＡＲ刺激剂激发精子ＡＲ，并对精子膜脂进行提取、分离和鉴定，同时，以相差显微镜评价精子ＡＲ％．结果为：（ｉ）ＧＡＢＡ刺激二磷酸磷脂酰肌醇（ＰＩＰ２）和一磷酸磷脂酰肌醇（ＰＩＰ）迅速降解，而磷脂酸（ＰＡ）增加；在５ｍｉｎ时ＰＩＰ２和ＰＩＰ明显下降，１０ｍｉｎ几近完成，而ＡＲ明显滞后，１５ｍｉｎ才达到峰值；（ｉｉ）Ａ２３１８７，Ｐ４和ＧＡＢＡ均可激发ＰＰＩ降解和ＡＲ增加，其能力依次为Ａ２３１８７≥ＧＡＢＡ；（ｉｉｉ）新霉素可明显地抑制Ｃａ２＋／ＧＡＢＡ或Ｐ４及Ａ２３１８７刺激ＰＩＰ２和ＰＩＰ降解、ＰＡ产生和ＡＲ增加；（　）ＰＰＩ降解需Ｃａ２＋参与．当ＥＧＴＡ或Ｃａ２＋通道阻滞剂（Ｌａ３＋）存在时，ＰＩＰ２和ＰＩＰ降解，ＰＡ产生和ＡＲ升高均被明显抑制．上述结果表明，天然激动剂ＧＡＢＡ或Ｐ４刺激豚鼠精子膜ＰＰＩ降解是引起ＡＲ的基础，该作用是通过Ｃａ２＋介导、激活膜磷脂酰肌醇特异磷脂酶Ｃ（ＰＩＣ）而完成的．

关键词： GABA 孕酮二磷酸磷脂酰肌醇一磷酸磷脂酰肌醇顶体反应精子豚鼠降解新霉素