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Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens

World Journal of Gastroenterology 2006年第33期12卷 5368-5374页

作者： Azam Roohi Yaghoub Yazdani Jalal Khoshnoodi Seyed Mohammad Jazayeri William F Carman Mahmood Chamankhah Manley Rashedan fazel shokri Department of Immunology School of Public Health Tehran University of Medical Sciences Tehran Iran Department of Virology School of Public Health Tehran University of Medical Sciences Tehran Iran Division of Virology Institute of Biomedical and Life Science University of Glasgow Glasgow United Kingdom Monoclonal Antibody Research Center Avesina Research Center Tehran Iran

AIM: To investigate the reactivity of a panel of 8 mouse anti-hepatitis B surface antigen (HBsAg) monoclonal antibodies (mAbs) using a collection of 9 recombinant HBsAg mutants with a variety of amino acid substitutions mostly located within the “a” ***: The entire HBs genes previously cloned into a mammalian expression vector were transiently transfected into COS7 cells. Two standard unmutated sequences of the ayw and adw subtypes served as controls. Secreted mutant proteins were collected and measured by three commercial diagnostic immunoassays to assess transfection efficiency. Reactivity of anti-HBs mAbs with mutated HBsAgs was determined by sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: Reactivity of anti-HBs mAbs with mutated HBsAgs revealed different patterns. While three mutants reacted strongly with all mAbs, two mutants reacted weakly with only two mAbs and the remaining proteins displayed variable degrees of reactivity towards different mAbs. Accordingly, four groups of mAbs with different but overlapping reactivity patterns could be envisaged. One group consisting of two mAbs (37C5-S7 and 35C6-S11) was found to recognize stable linear epitopes conserved in all mutants. Mutations outside the “a” determinant at positions 120 (P→S), 123(T→N) and 161(M→T) were found to affect reactivity of these ***: Our findings could have important implications for biophysical studies, vaccination strategies and immunotherapy of hepatitis B virus (HBV) mutants.

关键词： Hepatitis B surface antigen Hepatitis B virus Mutant Epitope mapping Vaccination Monoclonal antibody

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<i>In vitro</i>analysis of T cell responses induced by breast tumor cell lysate pulsed with autologous dendritic cells

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Advances in Bioscience and Biotechnology 2012年第2期3卷 126-136页

作者： Nowruz Delirezh Seyed Mohammad Moazzeni fazel shokri Mohammad Ali Shokrgozar Morteza Morteza Atri Hamid Karbassian Cancer Institute Imam Hospital Tehran University of Medical Science Tehran Iran Department of Cellular and Molecular Institute of Biotechnology Urmia University Urmia Iran Department of Immunology School of Health Tehran University of Medical Science Tehran Iran Department of Immunology School of Medical Science Trabiat Modares University Tehran Iran Department of Surgery Athieh Hospital Tehran Iran National Cell Bank of Iran Pasteur Institute of Iran Tehran Iran

In this In vitro study, T cell responses induced by breast tumor cell lysate pulsed monocyte-derived DCs were analyzed in terms of proliferation, specific cytotoxicity and cytokine-release in order to use in immunotherapeutic settings. Nylon wool enriched T lymphocytes from 5 patients with breast cancer stimulated In vitro with tumor cell lysate pulsed monocyte-derived DCs and their proliferation response were analyzed by [3H] thymidine uptake test. Specific cytotoxic activity of tumor antigen primed T cells after three rounds weekly stimulation was evaluated by flow cytometry, and interferon-γ (IFN-γ) and interleukin-4 (IL-4) cytokines release assay was carried out 24 hours after last stimulation in the supernatant of primed T cells using commercially available ELI-SA kits. T cell proliferation assay revealed that tumor cell lysate pulsed DCs could stimulate autologous T cell proliferation response with stimulation indices 4.9 - 30. T cell mediated cytotoxicity assay demonstrated that tumor antigen primed T cells could significantly kill autologous tumor cells more than normal cells (P γ and IL-4 in response to restimulation by antigen pulsed DCs which were dominated by IFN-γ production in 2 and IL-4 production in 3 out of 5 patients. Our result suggested that breast tumor antigen pulsed DCs could elicit effective specific antitumor T cell responses In vitro, therefore, tumor antigen pulsed DC vaccination may be considered as a novel strategy for immunotherapy of patients with breast cancer refractory to standard modalities.

关键词： Breast Lysate Dendritic Cells T Cell

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Effect of Ion Concentrations on the Characteristics of a Multi-Component Magnetized Plasma Sheath

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Plasma Science and Technology 2009年第6期11卷 642-646页

作者： M.M.HATAMI B.shokri Laser-Plasma Research Institute of Shahid Beheshti University G.C.EvinTehranIran Physical Department and Laser-Plasma Research Institute of Shahid Beheshti University G.C.EvinTehranIran

A hydrodynamic approach is used to investigate a three-component magnetized plasma sheath which consists of electrons and two species of positive ions. Assuming a phase space of one-dimensional spatial coordinate system and three-dimensional velocity coordinate system, the effect of different concentrations of positive ion species on some characteristics of the plasma sheath such as the velocity and density distribution of positive ion species and the electrostatic potential of this region is investigated. The calculated results show that the increase in the density ratio of positive ion species causes a decrease in both the ion velocities and the electrostatic potential of the sheath region. Also, it is shown that in the sheath region of a magnetized plasma consisting of only one positive ion species the bumps of the net density of charged particles disappears much faster. In addition, three-dimensional velocity of each positive ion species in the sheath region is plotted for different concentrations of positive ion species.

关键词： magnetized plasma plasma and electropositive plasma sheath hydrodynamic equations multi-component sheath

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Lattice Boltzmann simulation of natural convection in open end cavity with inclined hot wall

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Applied Mathematics and Mechanics(English Edition) 2015年第4期36卷 523-540页

作者： M.NAZARI H.shokri A.A.MOHAMAD Department of Mechanical Engineering Shahrood University of Technology Department of Mechanical Engineering Schulich School of EngineeringCentre for Environmental Engineering for Research and EducationUniversity of Calgary

Natural convection in an open end cavity with a hot inclined wall is simulated based on the lattice Boltzmann method （LBM）. The physics of flow and energy transfer in open end cavities are addressed when the hot wall is inclined. The combination of the two topics （open cavity and inclined walls） is the main novelty of the present study. The effects of the angle of the hot inclined wall on the flow field and heat transfer are thoroughly investigated. The Prandtl number is fixed to 0.71 （air）. The Rayleigh number and the angle of the hot inclined wall are varied in the range of 10^4 to 10^6 and 60° to 85°, respectively. The results are presented for two different aspect ratios, i.e., A = 1 and 2. The results obtained with the LBM are also compared with those of the finite volume method （FVM）. The predicted results of the LBM conform to those of the FVM. The results show that by increasing the angle of the hot inclined wall and the aspect ratio of the cavity, the average Nusselt number decreases. The trend of the local Nusselt number on the inclined wall is also discussed.

关键词： lattice Boltzmann method (LBM) natural convection open end cavity,inclined wall

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Recent tectonic activity in Central Alborz Mountain,Iran:morphometric analysis and knickpoint identification

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Journal of Mountain Science 2020年第12期17卷 3137-3161页

作者： Parisa shokri Maryam DEHBOZORGI Saeid HAKIMI ASIABAR Department of Earth Science Kharazmi UniversityTehran15719-14911Iran Department of Geology Lahijan BranchAzad UniversityGilan1616Iran

Alborz,as part of the Alpine-Himalayan orogeny belt,has been the result of the convergence of the Central Iranian and Eurasian plate since the late *** study area located in Central Alborz encompasses different Quaternary ***,the present study aimed to evaluate the effect of faults on river landforms through morphotectonic indices and the causes of knickpoints and knickzones in the Bedrock Rivers in part of Central *** this end,six morphometric indices such as longitudinal gradient(SL),hierarchical anomaly(Δα),the bifurcation index(R),hypsometric integral and hypsometric curve(Hi-Hc),relative relief(Bh),and basin shape(Bs) were calculated and analyzed in38 sub-basins of the ***,the area was divided into extremely high,high,medium,and low tectonic activity areas using the relative tectonic activity(IAT).Normalized steepness and concavity indices along with the longitudinal profiles of the streams were obtained using Matlab and Arc GIS *** on the findings,the existence of slope-break knickpoints at the mountain piedmont at the outlet of large rivers through active faults demonstrated that most of these landforms were created by movements along the main faults such as Khazar,Nusha,Soheil,Deylaman,Kashachal,representing high tectonic activity in the central and southern parts of the study ***,the values of these indices were extremely low and indicated low tectonic activity in the northern parts located in the embayment part of the Caspian ***,the findings revealed that these classes correspond well to the areas with landforms such as V-shaped and narrow valleys,gorges,deflected and offset streams,deformed Neogene,and Quaternary units that indicate the post-Neogene activity.

关键词： Morphotectonic Bedrock rivers Knickpoint Quaternary Fault Uplift Central Alborz

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Cutaneous anthrax associated with facial palsy:A case report

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Asian Pacific Journal of Tropical Medicine 2023年第8期16卷 379-382页

作者： Majid Ghafouri Seyed Mehran Mojtabaei Azar shokri Vector-borne Diseases Research Center North Khorasan University of Medical SciencesBojnurdIran Student Research Committee Bojnurd University of Medical SciencesBojnurddIran

Rationale:Anthrax is a zoonotic disease caused by spores of Gram-positive Bacillus anthracis,commonly affects mammals and in rare cases *** infection occurs accidentally through direct or indirect exposure to animal or their *** concerns:A 63-year-old man was referred to our hospital with flu-like symptoms and severe swelling and redness on the face,the roof of the mouth,and *** had a history of direct contact with a slaughtered mutton two days *** declared controlled diabetes,hypertension,hypertriglyceridemia,and heart *** were normal in lung high resolution CT,but multiple lymphadenopathies were seen in the *** axillary lymphadenopathy with a maximum sad of 23 mm and pleural effusion on the right side was *** scan of the nose and sinuses showed an increased density of polyps in the left maxillary *** were prepared from the patient's lesions and examined under a light *** shape with Streptococcus bacteria was ***:Anthrax co-infection with herpes systemic virus and Streptococcus ***:Multidrug therapy started with appropriate ***:The symptoms of the patient gradually *** patient was discharged without any ***:Cutaneous anthrax in endemic areas in patients with skin presentations and a history of contact with infected animals or products should be considered a differential *** is more important in mixed infections where the main cause of the problem may be hidden.

关键词： Case report Anthrax Cutaneous North East Iran

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Effect of Lactate on Epigenetic Regulation in the Development of Hepatitis B Virus-related Hepatocellular Carcinoma

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Journal of Clinical and Translational Hepatology 2022年第5期10卷 786-787页

作者： Shahab Mahmoudvand Somayeh shokri Research Center for Molecular Medicine Department of VirologySchool of MedicineHamadan University of Medical SciencesHamadanIran

Dear Editor,Hepatitis B virus(HBV)is a hepatotropic virus that can establish a persistent and chronic infection in *** hepatitis B(CHB)infection is associated with an increased risk of hepatic decompensation,cirrhosis,and hepatocellular carcinoma.1 Emerging studies show that lactate,an end product of anaerobic glycolysis,has an important role in metabolism beyond energy production and is also a regulator of the tumor microenvironment(TME)and immune cell function.2 It is noteworthy that lactate levels have been associated with mortality in patients,and large HBV surface proteins(LHBs)have been shown to reduce Pyruvate kinase M2(PKM2)activity and thereby increase overall glucose consumption and lactate production in hepatocytes.

关键词： Carcinoma metabolism infection

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Soft sensor design for hydrodesulfurization process using support vector regression based on WT and PCA

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Journal of Central South University 2015年第2期22卷 511-521页

作者： Saeid shokri Mohammad Taghi Sadeghi Mahdi Ahmadi Marvast Shankar Narasimhan Department of Chemical Engineering Iran University of Science and Technology (IUST) Tehran 16765?163 Iran Process & Equipment Technology Development Division Research Institute of Petroleum Industry (RIPI) Tehran 14665?137 Iran Department of Chemical Engineering IIT Madras Chennai 600036 India

A novel method for developing a reliable data driven soft sensor to improve the prediction accuracy of sulfur content in hydrodesulfurization(HDS) process was proposed. Therefore, an integrated approach using support vector regression(SVR) based on wavelet transform(WT) and principal component analysis(PCA) was used. Experimental data from the HDS setup were employed to validate the proposed model. The results reveal that the integrated WT-PCA with SVR model was able to increase the prediction accuracy of SVR model. Implementation of the proposed model delivers the best satisfactory predicting performance(EAARE=0.058 and R2=0.97) in comparison with SVR. The obtained results indicate that the proposed model is more reliable and more precise than the multiple linear regression(MLR), SVR and PCA-SVR.

关键词： soft sensor support vector regression principal component analysis wavelet transform hydrodesulfurization process

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Synthesis, Characterization, Spectroscopy and Study of Antimicrobial Properties of Two New Substituted Trichloroaluminate(Ⅲ) Complexes [AlCl_3X]^- (X = SCN^-, CN^-)

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Chinese Journal of Structural Chemistry 2013年第1期32卷 67-72页

作者： Zahra shokri Aghbolagh Shahriar Ghammamy Sajjad Sedaghat Shahram Jahandide Young Research Club Takestan Branch Islamic Azad University Takestan Iran Department of Chemistry Faculty of Science Islamic Azad University Malard Branch Malard Iran Department of Chemistry Faculty of Science Islamic Azad University Ardabil Branch Ardabil Iran

The synthesis and characterization of two new aluminate（Ⅲ） complexes with general formula K[AlCl3X] are reported. These compounds derived from aluminate trichloride and related salts. Potassium trichlorothiocyanoaluminate, PCTA, and potassium trichlorocyanatoalu-minate, PCCA, are two new ionic aluminate complexes. They can be easily synthesized in a nearly quantitative yield by using the direct reaction of AlCl3 and KX. The complexes were characterized by physico-chemical and spectroscopic methods. Theoretical calculations have been used for the extraction of structural and spectroscopic data of these new synthesized complexes. The antibacterial activities of such compounds were studied against the Staphylococcus aureus, Escherichia coli, Staphylococcus Epidermidis, Estreptococo B and Shigella.

关键词： aluminate compounds synthesis characterization PCTA PCCA theoretical calculation antibacterial activities

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Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola(Brassica napus L.)

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1403-1417页

作者： Reza shokri-Gharelo Ali Bandehagh Mohammad Anwar Hossain Department of Plant Breeding and Biotechnology University of TabrizTabriz51368Iran Department of Genetics and Plant Breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh

Drought stress is one of the most important abiotic stresses that plants face frequently in *** drought conditions,many morphological,physiological,and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth,yield,and *** expand our understanding of the molecular basis of the plant response to drought stress,the proteomic profile and protein-protein network of canola(Brassica napus L.)were *** focus was to show molecular mechanisms related to canola susceptibility to drought *** experiment used a completely randomized design,implemented in a hydroponic system under greenhouse *** impose drought stress,plants were exposed to Hoagland’s solution supplemented with polyethylene glycol(PEG)6000 for 7 *** drought stress resulted in 161reproducible protein spots in twodimensional electrophoresis of canola *** t-student test showed 21 differentially abundant proteins(DAP),of which 2 and 19 were up and down accumulated,*** spots identified as 1-aminocyclopropane-1-carboxylate oxidase and D-2-hydroxyglutarate dehydrogenase showed an increased abundance of 2.11 and 1.77,*** extended protein-protein interaction of differentially abundant proteins and KEGG analysis showed 47 pathways directly and indirectly associated with canola response to drought *** with increased abundance were associated with amino acid and signaling processes,whereas DAPs with decreased abundance were mostly connected with pathways responsible for energy *** results of the study will help to elucidate further the molecular events associated with the susceptibility to drought stress in canola.

关键词： Abiotic stress differentially abundant protein molecular function proteomics susceptibility

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