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Chemically Induced Mutants of Brassica oleracea var. botrytisMaintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation

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American Journal of Plant Sciences 2013年第3期4卷 498-507页

作者： fazal hadi Michael P. Fuller Plant Physiology Laboratory School of Biological and Biomedical Sciences University of Plymouth Plymouth UK

Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea ***) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of cauliflower were created and screened for drought and salt stress tolerance. The highly tolerant mutants were selected, regenerated by tissue culture techniques, screened again for drought and salt tolerance under in-vitro and in-vivo conditions, correlated the response of in-vitro and in-vivo plants within a clone. Free proline levels in clones were correlated with stress tolerance. Results confirmed the persistence of mutations in clones with enhanced resistance levels to stresses over control plants. The regenerated in-vitro and in-vivo plants within a clone showed a positive significant correlation for drought (R2 = 0.663) and salt (R2 = 0.647) resistance that confirms the stability of mutation in clones after generations. Proline showed a positive and significant correlation with drought (R2 = 0.524) and salt (R2 = 0.786) tolerance. Conclusively, drought and salt resistance can be successfully enhanced in cauliflower by chemical mutagenesis. Further molecular analysis is recommended to study these mutants.

关键词： Drought Salt Stress Resistance Proline In-Vitro In-Vivo Cauliflower

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Cadmium (Cd) Removal from Saline Water by Veronica anagallisand Epilobium laxumPlants in Hydroponic System

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Agricultural Sciences 2014年第11期5卷 935-944页

作者： fazal hadi Ayaz Ahmad Nasir Ali Department of Biotechnology Faculty of Biological Sciences University of Malakand Khyber Pakhtunkhwa Pakistan Department of Botany Faculty of Biological Sciences University of Malakand Khyber Pakhtunkhwa Pakistan

Present study was conducted to investigate the Cadmium (Cd) phytoextraction potential of two plants (Veronica anagallis-aquatic and Epilobium laxum Royle) for Cd removal from induced saline water. In hydroponic system, various concentrations of the Cd (50, 100, and 150 ppm) and NaCl salt (1000, 3000, and 6000 ppm) were used alone and in various combinations to evaluate the effect of salt (NaCl) concentrations on Cd absorption and accumulation in Veronica anagallis and Epilobium plants. The Cd at higher concentrations (100 and 150 ppm) significantly reduced the growth and biomass of both plants and addition of salt (NaCl) to growth media (Hoagland solution) further reduced the growth. The Cadmium (Cd) translocation factor (TF) of Epilobium plant was more than one (1), while the Veronica plant showed translocation factor less than 0.5. Veronica plant showed higher Bio-concentration factor (BCF) as more than 3.5 and Epilobium plant demonstrated Bio-concentration factor less than 1 (BCF 1 is a threshold limit for a plant to be hyper-accumulator of Cd). Conclusively, the Veronica anagallis plant is reported as Cd hyper-accumulator, while Epilobium laxum plant as non hyper-accumulator on the basis of BCF values in the present findings. Further study on Veronica and Epilobium plants is recommended.

关键词： Cadmium Saline Water Metal Phytoaccumulation Veronica anagallis Epilobium laxum

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