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GPS-based fine-scale mapping surveys for schistosomiasis assessment: a practical introduction and documentation of field implementation

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Infectious Diseases of Poverty 2022年第1期11卷 88-89页

作者： Lydia Trippler Mohammed Nassor Ali Shaali Makame.ame.Said Mohammed Ali fatma Kabole Jan Hattendorf Stefanie Knopp Swiss Tropical and Public Health Institute Kreuzstrasse 24123AllschwilSwitzerland 不详

Background Fine-scale mapping of schistosomiasis to guide micro-targeting of interventions will gain importance in elimination settings,where the heterogeneity of transmission is often *** mobile applications offer new opportunities for disease *** provide a practical introduction and documentation of the strengths and shortcomings of GPS-based household identification and participant recruitment using tablet-based applications for fine-scale schistosomiasis mapping at sub-district level in a remote area in Pemba,*** A community-based household survey for urogenital schistosomiasis assessment was conducted from November 2020 until February 2021 in 20 small administrative areas in *** the survey,1400 housing structures were prospectively and randomly selected from shapefile *** identify pre-selected structures and collect survey-related data,field enumerators searched for the houses’geolocation using the mobile applications Open Data Kit(ODK)and *** number of inhabited and uninhabited structures,the median distance between the pre-selected and recorded locations,and the dropout rates due to non-participation or non-submission of urine samples of sufficient volume for schistosomiasis testing was *** Among the 1400 randomly selected housing structures,1396(99.7%)were identified by the *** median distance between the pre-selected and recorded structures was 5.4 m.A total of 1098(78.7%)were residential *** them,99(9.0%)were dropped due to continuous absence of residents and 40(3.6%)households refused to *** 797(83.1%)among the 959 participating households,all eligible household members or all but one provided a urine sample of sufficient *** The fine-scale mapping approach using a combination of ODK and an offline navigation application installed on tablet computers allows a very precise identification of housing *** due to non-residential housin

关键词： Urogenital schistosomiasis Control Elimination Intervention Fine-scale mapping Interruption of transmission Micro-mapping Precision mapping Wayfinding Zanzibar

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Performance of a real-time PCR approach for diagnosing Schistosoma haematobium infections of different intensity in urine samples from Zanzibar

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Infectious Diseases of Poverty 2020年第5期9卷 22-34页

作者： Dominique Keller Julian Rothen Jean-Pierre Dangy Corina Saner Claudia Daubenberger Fiona Allan Shaali M.ame.Said M.Ali fatma Kabole Jan Hattendorf David Rollinson Ralf Seyfarth Stefanie Knopp Biolytix AG Benkenstrasse 2544108 WitterswilSwitzerland Swiss Tropical and Public Health Institute Socinstrasse 574002 BaselSwitzerland University of Basel Petersplatz 14001 BaselSwitzerland Wolfson Wellcome Biomedical Laboratories Department of Life SciencesNatural History MuseumCromwell RoadLondon SW75BDUK Public Health Laboratory Ivo de Carneri P.O.Box 122Chake-ChakePembaUnited Republic of Tanzania Neglected Diseases Programme Ministry of HealthP.O.Box 236Zanzibar TownUngujaUnited Republic of Tanzania

Background:Efforts to control and eliminate schistosomiasis have accelerated over the past *** parasite burden,associated morbidity and egg excretion decrease,diagnosis with standard parasitological methods becomes *** assessed the robustness and performance of a real-time PCR(qPCR)approach in comparison with urine filtration microscopy and reagent strip testing for the diagnosis of Schistosoma haematobium infections of different ***:The robustness of DNA isolation and qPCR was validated in eight laboratories from Europe and ***,792 urine samples collected during cross-sectional surveys of the Zanzibar Elimination of Schistosomiasis Transmission(ZEST)project in 2012-2017 were examined with qPCR in *** sensitivity of the qPCR was calculated at different infection intensity categories,using urine filtration microscopy as reference ***'s rank correlation between Ct-values and *** egg counts was assessed and Ct-value percentiles for infection intensity categories ***:*** Dra1 DNA-positive samples were identified correctly in all eight *** of urine samples from Zanzibar revealed Dra1 DNA in 26.8%(212/792)by qPCR,*** eggs in 13.3%(105/792)by urine filtration,and microhaematuria in 13.8%(109/792)by reagent *** of the qPCR increased with augmenting egg counts:80.6%(29/36)for counts between 1 and 4 eggs,83.3%(15/18)for counts between 5 and 9 eggs,100%(23/23)for counts between 10 and 49 eggs,and 96.4%(27/28)for counts of 50+*** was a significant negative correlation between Ct-values and egg counts(Spearman's rho=-0.49,P<0.001).Seventy-five percent of the Ct-values were≥33 in the egg-negative category,<31 in the light intensity category,and<24 in the heavy intensity ***:While the sensitiivity of the qPCR was^80%for very light intensity infections(egg counts<10),in general,the Dra1 based qPCR assay detected t

关键词： Control Diagnosis Dra 1 Elimination Microhaematuria Real-time PCR Schistosoma haematobium Surveillance Urine filtration Zanzibar

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