Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemoth...
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Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.
Efforts to improve crop yields with efficient use of fertilizers are needed to guarantee global food security.Enhanced slow-release fertilizer systems(SRFs)encapsulated in biodegradable matrices are being developed to...
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Efforts to improve crop yields with efficient use of fertilizers are needed to guarantee global food security.Enhanced slow-release fertilizer systems(SRFs)encapsulated in biodegradable matrices are being developed to address this global concern.From a wide range of strategies for SRFs development,we explored a natureinspired solution based on the plant cuticle model and its function as a membrane for water and nutrient transport control.Here,Candelilla wax,extracted from Candelilla wild plants(Euphorbia antisyphilitica),is studied as a renewable slow-release matrix for fertilizers encapsulated by a modified spray chilling process.From this process,microencapsulates containing 40 wt%of phosphorus fertilizer are obtained with distinctive sizes and chemical characteristics,presenting a slow-release behavior.Considering the abovementioned features,novel insights into fertilizer release mechanisms based on plant cuticle models are discussed.
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