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Prevalence and Associated Factors of Anemia among Pregnant Women Receiving Antenatal Care (ANC) at Fatima Hospital in Jashore, Bangladesh: A Cross-Sectional Study

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Food and Nutrition Sciences 2019年第9期10卷 1056-1071页

作者： Suzon Ahmed Md. Abdullah Al Mamun Niaz Mahmud Nisat farzana Mosammat Sadia Akther Sathi Biplob Kumar Biswas Amit Datta Tanvir Ahmad Department of Nutrition and Food Technology Faculty of Applied Science and Technology Jashore University of Science and Technology Jashore Bangladesh

Since anemia is one of the most skyrocketed public health problems worldwide, an investigation has thus been conducted to assess the prevalence and associated factors of anemia among the pregnant women receiving antenatal care (ANC) at Fatima Hospital in Jashore, Bangladesh. Facility-based cross-sectional study was carried out among 384 pregnant women at Fatima hospital, Bangladesh from February to April, 2019. Data were obtained using a structured questionnaire and participant’s current medical record card. Binary logistic regression analysis was used to identify factors associated with anemia and a P-value < 0.05 was considered as statistically significance. The overall prevalence of anemia among pregnant women was 58.9% (226/384). Out of 226 anemic pregnant mothers, 36.3% (82/226) were mildly anemic, 62.4% (141/226) were moderately anemic and 1.3% (3/384) was severely anemic. Binary logistic regression analysis revealed that the following variables were significantly associated with anemia: Monthly family income (11,000 - 20,000 Taka) [AOR (95% CI) = 0.45 (0.21 - 0.98)], Family size (joint) [AOR (95% CI) = 1.59 (1.03 - 2.45)], Gestational age (third trimester) [AOR (95% CI) = 2.18 (1.40 - 3.40)], birth spacing < 2 years [AOR (95% CI) = 2.87 (1.51 - 5.44)], Excessive blood loss during previous surgery (Yes) [AOR (95% CI) = 2.08 (1.14 - 4.17)], Food group eaten 24 hours (1 - 4 groups) [AOR (95% CI) = 3.43 (1.84 - 6.39)], Breakfast regularly (No) [AOR (95% CI) = 3.64 (1.36 - 9.75)]. The results obtained showed that the prevalence of anemia among pregnant women is severe at Fatima Hospital in Jashore, Bangladesh.

关键词： Anemia Antenatal Care (ANC) Socio-Demographic Characteristics Maternal Characteristics Dietary Characteristics Nutritional Characteristics

Predictors of Meningitis in Under-Fifteen Children Attending an Intensive Care Unit of an Urban Large Diarrheal Disease Hospital in Bangladesh

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Food and Nutrition Sciences 2014年第2期5卷 169-176页

作者： farzana Afroze Tahmeed Ahmed Shafiqul Alam Sarker Abu S. G. Faruque Hasan Ashraf Pradip Kumar Bardhan Sumon Kumar Das Mohammod Jobayer Chisti Centre for Nutrition and Food Security (CNFS) International Centre for Diarrhoeal Disease Research Dhaka Bangladesh Centre for Nutrition and Food Security (CNFS) International Centre for Diarrhoeal Disease Research Dhaka Bangladesh School of Population Health The University of Queensland Brisbane Australia.

Background: Data are lack on predicting features of meningitis in diarrheal children although the great clinical importance. Objective: To evaluate clinical and laboratory features in predicting meningitis in under-fifteen children having diarrheal illnesses. Methods: Children aged 1 month to 15 years, admitted in the ICU of the Dhaka Hospital of icddr, b between March 2011 and February 2012 with fever and seizure or altered consciousness and having LP done were enrolled into this analysis. Those children who had abnormal CSF findings [pleocytosis (normal range of leukocyte, 0 - 10/mm3) and/or elevated protein (normal range, 0.10 - 0.45 mg/dl) and low glucose (normal value, 60% of corresponding blood glucose)] were defined as meningitis. Comparison was made between children with (cases = 17) and without meningitis (controls = 66) from our study children. Data were retrospectively collected from SHEBA, an online database system of the Dhaka Hospital of icddr, b. Results: Death was significantly higher among the cases compared to the controls (29% vs. 3%, p = 0.003). In logistic regression analysis, after adjusting for potential confounders, cases frequently had hypoxemia (95% CI 1.55 - 21.93), absent peripheral pulse (95% CI 1.95 - 27.13) and neutrophilia (95% CI 1.13 - 17.00). Conclusion: Our data suggest that children with meningitis had higher case fatality rate. Simple independent predictors of meningitis such as hypoxemia, absent peripheral pulse, and neutrophilia may help clinicians to initiate early and prompt management in order to curve lifelong sequel due to meningitis and death in such patient population especially those in resource poor settings.

关键词： Children Diarrhea Hypoxemia Meningitis Neutrophilia Peripheral Pulse

ABO Blood Groups and Their Relationship with Coagulation Factor VIII in Healthy Adults

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Journal of Biosciences and Medicines 2021年第9期9卷 49-58页

作者： Afrina Binte Azad Qazi Shamima Akhter Mohammad Aminul Islam farzana Yeasmin Mukta Lily Afroz Khushbun Nahar Layla Tahmina Akter Shamanta Islam A. Z. M. Adnan National Institute of Traumatology and Orthopaedics Rehabilitation Dhaka Bangladesh Department of Physiology Dhaka Medical College and Hospital Dhaka Bangladesh Department of Orthopaedics National Institute of Traumatology and Orthopaedics Rehabilitation Dhaka Bangladesh Department of Cardiology Kurmitola General Hospital Dhaka Bangladesh Department of Physiology Mymensingh Medical College and Hospital Mymensingh Bangladesh Department of Physiology Ibrahim Medical College and Hospital Dhaka Bangladesh Bhairab Upazila Health Complex Kishoreganj Bangladesh Department of Nephrology Chattogram Medical College and Hospital Chattogram Bangladesh

Background: ABO blood group distribution defers with racial and geographic variations. They are related to diseases like cardiovascular diseases, cerebral thromboembolism. ABO blood group system may influence coagulation factor VIII which may increase the future risk of thrombosis. Aim: To assess the relation of ABO blood group with coagulation factor VIII in healthy adults. Material and Methods: A prospective type of analytical cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2019 to June 2020. After obtaining ethical clearance, a total of 190 healthy adults were selected from different areas of Dhaka city based on inclusion and exclusion criteria, with ages ranging from 18 - 45 years. The subjects were interviewed and detailed history regarding personal, family, medical and drug were taken. Prior to sample collection, informed written consent was taken from the participants. Individuals of blood group A were selected as group A, blood group B as group B, blood group AB as group AB and blood group O as group O. Coagulation factor VIII was measured in the Department of Hematology and BMT Unit, Dhaka Medical College Hospital, Dhaka. Blood grouping was done in the Department of Physiology, Dhaka Medical College, Dhaka. Statistical Analysis: For statistical analysis, ONE way ANOVA followed by Bonferroni test were considered using SPSS 25.0 version. Results: In this study, blood group B was most common (33.2%). Coagulation factor VIII was significantly higher (p Conclusions: It can be concluded that blood group A, B, AB individuals may have more chance of thrombosis due to significan

关键词： ABO Blood Group Coagulation Factor VIII Healthy Adults

The Possibility of Nucleic Acids to Act as Anti-Viral Therapeutic Agents—A Review

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Open Journal of Medical Microbiology 2021年第3期11卷 198-248页

作者： Md. Selim Reza farzana Mim Mohammad Rezaul Quader Mohammad Jahidur Rahman Khan Md. Sabir Hossain Kazi Rasel Uddin Salina Akter Sharmin Rahman Sumon Roy Md. Abdullah Rumman RT-PCR LAB Bangabandhu Sheikh Mujib Medical College Faridpur Bangladesh Department of Biochemistry and Molecular Biology Jahangirnagar University Dhaka Bangladesh Department of Biochemistry Bangabandhu Sheikh Mujib Medical College Faridpur Bangladesh Department of Microbiology Shaheed Suhrawardy Medical College Dhaka Bangladesh Department of Pharmacology Ibrahim Medical College Dhaka Bangladesh

For just about 30 years, researchers have considered the likelihood to utilize nucleic acids as antiviral therapeutics. In principle, small single-stranded nucleotide sequence (oligonucleotide) could hybridize to a particular gene or messenger RNA and diminish transcription or translation, respectively, in this manner decreasing the amount of protein that is synthesized. Until now, an incredible number of antisense oligonucleotides, double-stranded oligonucleotides, aptamers, ribozymes, deoxyribozymes, interfering RNAs, chimeric RNA-DNA molecules, antibody genes has been created artificially and applied effectively for comprehension and manipulating biological processes and in clinical preliminaries to treat a variety of diseases. Their versatility and potency make them similarly fit candidates for fighting viral infections. However, troubles with their efficiency, off-target effects, toxicity, delivery, and stability halted the development of nucleic acid-based therapeutics that can be utilized in the clinic. The potential for nucleic acid therapeutic agents is significant and is quite recently beginning to be realized. In this review, we have summarized some of the recent advancements made in the area of nucleic acid based therapeutics and focused on the methods of their delivery and associated challenges.

关键词： Antisense Oligonucleotides Aptamers Ribozymes Deoxyribozymes RNA Interference

Renewed Focus on the Threat of Cross-Contamination in Molecular Laboratories: Notes from the COVID-19 Testing Experience in Bangladesh

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Advances in Infectious Diseases 2021年第4期11卷 357-365页

作者： Mohammad Jahidur Rahman Khan Ruksana Raihan S. M. Rashed Ul Islam Nusrat Mannan Mohammad Jamal Uddin Nazia Hasan Khan Tanzila Rawnuck Amirul Huda Bhuiyan farzana Mim Md. Selim Reza Department of Microbiology Shaheed Suhrawardy Medical College Dhaka Bangladesh Department of Microbiology US-Bangla Medical College Dhaka Bangladesh Department of Virology Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh Department of Microbiology & Mycology National Institute of Preventive and Social Medicine Dhaka Bangladesh RT-PCR LAB United Hospital Limited Dhaka Bangladesh Department of Pathology & Microbiology Dhaka Dental College Dhaka Bangladesh Department of Virology Dhaka Medical College Dhaka Bangladesh Department of Biochemistry & Molecular Biology Jahangirnagar University Dhaka Bangladesh RT-PCR LAB Bangabandhu Sheikh Mujib Medical College Faridpur Bangladesh

Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one government molecular laboratory where real-time RT-PCR would be performed to diagnose SARS-CoV-2 infection. With the increasing number of suspected cases requiring confirmation diagnostic testing, there is a requirement to expand capacity for large-scale testing quickly. The government of Bangladesh established over 100 molecular laboratories within one year to test COVID-19. To expand the testing capacity, the government was compelled to recruit laboratory staff with limited experience and technical expertise, especially in molecular assays, to process specimens, interpret results, troubleshoot. As a result, the risk of diagnostic errors, such as cross-contamination, increased, potentially undermining the efficacy of public health policies, public health response, surveillance programs, and restrictive measures aimed toward containing the outbreak. In this piece, we discuss the different sources of cross-contamination in the COVID-19 RT-PCR laboratories and proffer practical preventive measures to avoid them.

关键词： SARS-CoV-2 RT-PCR Cross-Contaminations Quality Control COVID-19 False Positive

Chi-Square Distribution: New Derivations and Environmental Application

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Journal of Applied Mathematics and Physics 2019年第8期7卷 1786-1799页

作者： Thomas M. Semkow Nicole Freeman Umme-farzana Syed Douglas K. Haines Abdul Bari Abdul J. Khan Kimi Nishikawa Adil Khan Adam G. Burn Xin Li Liang T. Chu Wadsworth Center New York State Department of Health Albany NY USA Department of Environmental Health Sciences University at Albany State University of New York Rensselaer NY USA Averill Park Central School District Averill Park NY USA

We describe two new derivations of the chi-square distribution. The first derivation uses the induction method, which requires only a single integral to calculate. The second derivation uses the Laplace transform and requires minimum assumptions. The new derivations are compared with the established derivations, such as by convolution, moment generating function, and Bayesian inference. The chi-square testing has seen many applications to physics and other fields. We describe a unique version of the chi-square test where both the variance and location are tested, which is then applied to environmental data. The chi-square test is used to make a judgment whether a laboratory method is capable of detection of gross alpha and beta radioactivity in drinking water for regulatory monitoring to protect health of population. A case of a failure of the chi-square test and its amelioration are described. The chi-square test is compared to and supplemented by the t-test.

关键词： Mathematical Induction Laplace Transform Gamma Distribution Chi-Square Test Gross Alpha-Beta Drinking Water

牛疱疹病毒-1型被膜蛋白pUL21是二次被膜和病毒生长必需蛋白

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牛疱疹病毒-1型被膜蛋白pUL21是二次被膜和病毒生长必需蛋白

中国树花可培养内生真菌分离、鉴定及系统发育分析

第五届全国人畜共患病学术研讨会

作者： farzana Shahin Sohail Raza 胡长敏陈颖钰陈焕春郭爱珍华中农业大学农业微生物学国家重点实验室华中农业大学动物医学院华中农业大学湖北省兽医流行病学国际科技合作基地

牛传染性鼻气管炎是危害养牛业的重要传染病,由牛疱疹病毒1型(BoHV-1)引起。被膜病毒可能在病毒形成中具有重要作用,本研究旨在鉴定病毒被膜蛋白pUL21在病毒形成中的功能。首先利用BAC技术构建了牛疱疹病毒1型UL21突变株vBoHV-1-△UL21...

牛传染性鼻气管炎是危害养牛业的重要传染病,由牛疱疹病毒1型(BoHV-1)引起。被膜病毒可能在病毒形成中具有重要作用,本研究旨在鉴定病毒被膜蛋白pUL21在病毒形成中的功能。首先利用BAC技术构建了牛疱疹病毒1型UL21突变株vBoHV-1-△UL21和回补株△UL21R,在细胞培养模型上进行生长特征分析,发现UL21突变株病毒滴度显著降低,空斑变小。利用HA蛋白标记野生株和突变株的UL35蛋白,然后在细胞上跟踪病毒成熟过程。激光共取焦显微

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生物技术 2018年第2期28卷 142-150页

作者：菲尔则娜·艾尔肯靳文婷帕孜来提·拜合提新疆大学生命科学与技术学院新疆乌鲁木齐830046 新疆大学"中国西北干旱区地衣研究中心" 新疆乌鲁木齐830046

[目的]了解中国树花内生真菌的多样性及其系统发育关系。[方法]通过常规组织分离法,采用马铃薯葡萄糖琼脂培养基(PDA)分离培养中国树花内生真菌,对内生真菌的菌落、菌丝和孢子进行观察并结合r DNA-ITS序列的PCR扩增测序,对获得的ITS序... 详细信息

[目的]了解中国树花内生真菌的多样性及其系统发育关系。[方法]通过常规组织分离法,采用马铃薯葡萄糖琼脂培养基(PDA)分离培养中国树花内生真菌,对内生真菌的菌落、菌丝和孢子进行观察并结合r DNA-ITS序列的PCR扩增测序,对获得的ITS序列通过Gen Bank数据库搜索同源序列并进行分析确定其分类地位并构建系统发育树。[结果]从中国树花中分离得到可培养内生真菌51株,分属4纲、5目、7科、12属,其中青霉属(Penicillium sp.)和曲霉属(Aspergillus sp.)总相对分离率分别为47%、18%,为中国树花内生真菌优势属。[结论]首次对中国树花进行内生真菌的分离,并确定其分类地位,对分离得到的51株内生真菌进行合并相同菌落形态和显微结构,共获得18株内生真菌,通过系统发育分析发现这18株聚为5大类。

关键词：中国树花内生真菌 ITS序列

Discovery of Simple Sequence Repeat Markers through Transcriptome Analysis of Baccaurea motleyana

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Journal of Food Science and Engineering 2020年第2期10卷 33-46页

作者： Khairun Hisam Nasir Muhammad Fairuz Mohd Yusof Mohd Shahril Firdaus Abdul Razak Siti NorsaidahIbrahim Mira farzana Mohamad Moktar Khadijah Awang Mohd Shukri Mat Ali Centre for Molecular Marker Discovery and Validation(CMDV) Agri-Omic dan BioinformaticsBiotechnology and Nanotechnology Research CentreMalaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia Industrial Crop Research Centre Malaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia Horticulture Research Centre Malaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia

Baccaurea motleyana(rambai)is underutilized fruits that are native to Malaysia,Indonesia and *** this study,a total of 54,779 unigenes identified from rambai transcriptome were used for simple sequence repeat(SSR)analysis by MIcroSAtellite(MISA).A total of 20,420 SSRs were found to be distributed within 37.27%of the total number of *** repeats represented the main type,accounting for 64.04%,followed by trinucleotide repeats(20.28%)and dinucleotide repeats(19.94%).Gene annotation to seven databases has a success ratio of 68.53%(National Center for Biotechnology Information(NCBI)protein sequences),53.68%(NCBI nucleotide sequences),27.43%(Kyoto Encyclopedia of Genes and Genome Ortholog),56.0%(SwissProt),52.44%(Protein family),53.99%(Gene Ontology)and 26.44%(Kluster of Orthologous Group).Further rambai SSRs were randomly selected and validated to ***(rambai),***(tampoi),***(jentik-jentik),***(pupor)and ***(setambun).

关键词： Baccaurea SSR transcriptome gene annotation and SSR validation