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Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 197-202页

作者： fariha masood siddiqui Muhammad Akram Nighat Nourccn Zobia Noreen Habib Bokhari Department of Biosciences.COMSATS Institute of Information Technology

Objective:To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni(***) isolates from clinical human diarrheal infections,cattle and healthy ***:Antibiotic sensitivity patterns of *** isolates were determined by Kirby Bauer Disc Diffusion *** isolates were then subjected to virulence profiling for the detection of map A(membrane-associated protein).cadF(fibronectin binding protein).wlaN(beta-1.3-galaclosyltransferase) and neu AB(sialic acid biosynthesis gene).Further *** isolates were grouped by random amplification of polymorphic DNA(RAPD) ***:A total of436 samples from poultry(n=88).cattle(n=216) and humans(n=132) from different locations were *** revealed percentage of *** isolates were 35.2%(31/88).25.0%(54/216) and 11.3%(15/132) among poultry,cattle and clinical human samples *** susceptibility results showed that similar resistance patterns to cephalothin was ie.87.0%,87.1%and 89%among humans,poultry and cattle respectively,followed by sulfamethoxazolc+trimcthoprim 40.0%,38.7%and 31.0%in humans,poultry and cattle and Ampicillin 40%,32%and 20%in humans,poultry and cattle ***-lactamase activity was detected in 40.00%humans.20.37%cattle and 32.25%in poultry *** *** and mapA were present in all poultry,cattle and human C jejuni *** was not detected in any isolate and neu AB was found in 9/31(36%) poultry *** profiling results suggested high diversity of *** ***:Detection of multidrug resistant *** strains from poultry and cattle is alarming as they can be potential hazard to ***,predominant association of virulence factors,cadF and map A(100%each) in *** isolates from all sources and neuAB(36%) with poultry isolates suggest the potential source of transmission of diverse types of *** to humans.

关键词： Campylobacter jejuni Antibiotic susceptibility Virulence genes PCR RAPD

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EXACT SOLUTIONS FOR MAGNETOHYDRODYNAMIC FLOW IN A ROTATING FLUID

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Acta Mechanica Sinica 2002年第3期18卷 244-251页

作者： S. Asghar masood Khan A.M. siddiqui T. Hayat Department of Mathematics Quaid-i-Azam University Islamabad Pakistan Department of Mathematics Pennsylvania State University York Campus 1031 Edgecomb Avenue New York PA 17403 USA

An analytical solution is obtained for the flow due to solid-body rotations an oscillating porous disk and of a fluid at infinity. Neglecting the induced magnetic field, the effects of the transversely applied magnetic field on the flow are studied. Further, the flow confined between two disks is also discussed. It is found that an infinite number of solutions exist for the flow confined between two disks.

关键词： rotating fluid porous disk double disks magnetohydrodynamic flow

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Flow Injection Chemiluminescence Method for Nalbuphine Hydrochloride in Pharmaceutical Formulations Using Tris(2,2′-bipyridyl)ruthenium(II)Chloride-diperiodatocuprate(III)Reaction

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Chemical Research in Chinese Universities 2021年第3期37卷 629-638页

作者： AHMED Khan MUHAMMAD Asghar MOHAMMED Yaqoob masood Ahmed siddiqui SAMAR Ali Department of Chemistry University of BalochistanSariab RoadQuetta87300Pakistan

A sensitive and selective method employing chemiluminescence(CL)coupled with flow injection(FI)is reported for nalbuphine hydrochloride(NAL)assay in pharmaceutical *** enhancement effect of NAL on the CL reaction between tris(2,2′-bipyridyl)ruthenium(II)chloride-diperiodatocuprate(III){Ru[(bpy)_(3)]^(2+)-Cu(III)complex}in acidic medium is used as analytical *** optimal conditions of the CL reaction were sulfuric acid 1.0×10^(−3) mol/L,Ru[(bpy)s]2+7.5×10^(−5) mol/L,Cu(III)/Ag(III)complexes 4.0×10^(−4)/5.0×10^(−4) mol/L,sample loop volume of 120µL and flow rate of 2.5 mL/*** sensitivities of the method in terms of detection(S/N=3)and quantification(S/N=10)limits are 5×10^(−4) and 0.001 ppm(1 ppm=1 mg/L),*** linear response of the instrument in the form of CL intensity with respect to NAL concentration is over the range 0.001-15.0 ppm(R^(2)=0.9999)with relative standard deviation from 0.8%to 3.2%and injection throughput of 120 injection/*** applications of the method include the quantitative analysis of NAL in pharmaceutical injection *** and the average results of the proposed method are not significantly different from the results of a reported method by applying F-and paired student *** most likely CL reaction mechanism is written in accordance with spectrophotometric and CL studies.

关键词： Ru[(bpy)_(3)]^(2+) Cu(III)/Ag(III)complex Chemiluminescence Flow injection analysis Nalbuphine hydrochloride Pharmaceutical

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Intelligent Decision Support System for COVID-19 Empowered with Deep Learning

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Computers, Materials & Continua 2021年第2期66卷 1719-1732页

作者： Shahan Yamin siddiqui Sagheer Abbas Muhammad Adnan Khan Iftikhar Naseer Tehreem masood Khalid masood Khan Mohammed A.Al Ghamdi Sultan H.Almotiri School of Computer Science NCBA&ELahore54000Pakistan School of Computer Science Minhaj University LahoreLahore54000Pakistan Department of Computer Science Lahore Garrison UniversityLahore54792Pakistan Department of Computer Science&Information Technology Superior UniversityLahore54000Pakistan Computer Science Department Umm Al-Qura UniversityMakkah City715Saudi Arabia

The prompt spread of Coronavirus(COVID-19)subsequently adorns a big threat to the people around the *** evolving and the perpetually diagnosis of coronavirus has become a critical challenge for the healthcare *** increase of COVID-19 has rendered the necessity to detect the people who are more likely to get ***,the testing kits for COVID-19 are not available to deal it with required proficiency,along with-it countries have been widely hit by the COVID-19 *** keep in view the need of hour asks for an automatic diagnosis system for early detection of *** would be a feather in the cap if the early diagnosis of COVID-19 could reveal that how it has been affecting the masses *** to the apparent clinical research,it has unleashed that most of the COVID-19 cases are more likely to fall for a lung *** abrupt changes do require a solution so the technology is out there to pace up,Chest X-ray and Computer tomography(CT)scan images could significantly identify the preliminaries of COVID-19 like lungs *** scan and X-ray images could flourish the cause of detecting at an early stage and it has proved to be helpful to radiologists and the medical *** unbearable circumstances compel us to flatten the curve of the sufferers so a need to develop is obvious,a quick and highly responsive automatic system based on Artificial Intelligence(AI)is always there to aid against the masses to be prone to *** proposed Intelligent decision support system for COVID-19 empowered with deep learning(ID2S-COVID19-DL)study suggests Deep learning(DL)based Convolutional neural network(CNN)approaches for effective and accurate detection to the maximum extent it could be,detection of coronavirus is assisted by using X-ray and CT-scan *** primary experimental results here have depicted the maximum accuracy for training and is around 98.11 percent and for validation it comes out to be approxim

关键词： COVID-19 deep learning convolutional neural network CT-scan X-ray decision support system ID2S-COVID19-DL

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