检索结果-南通市图书馆

Aberrant DNA methylation reprogramming during induced pluripotent stem cell generation is dependent on the choice of reprogramming factors

引用

Cell Regeneration 2014年第1期3卷 30-43页

作者： Aline C Planello Junfeng Ji Vivek Sharma Rajat Singhania faridah mbabaali Fabian Müller Javier A Alfaro Christoph Bock Daniel D De Carvalho Nizar N Batada Campbell Family Cancer Research Institute Ontario Cancer InstitutePrincess Margaret Cancer CentreUniversity Health NetworkTorontoON M5G 2 M9Canada Department of Morphology Piracicaba Dental SchoolUniversity of CampinasPiracicabaSPBrazil Ontario Institute for Cancer Research TorontoOntario M4G 0A3Canada Center of Stem Cell and Developmental Biology Zhejiang UniversityHangzhouZhejiang Province 310058China National Cancer Institute NIHBethesdaMD 20892USA Department of Medical Biophysics University of TorontoTorontoON M5G 2 M9Canada Max Planck Institute for Informatics Campus E1.466123 SaarbrückenGermany CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Lazarettgasse 141090 ViennaAustria Department of Laboratory Medicine Medical University of ViennaWähringer Gürtel 18-201090 ViennaAustria

The conversion of somatic cells into pluripotent stem cells via overexpression of reprogramming factors involvesepigenetic remodeling. DNA methylation at a significant proportion of CpG sites in induced pluripotent stem cells(iPSCs) differs from that of embryonic stem cells (ESCs). Whether different sets of reprogramming factors influencethe type and extent of aberrant DNA methylation in iPSCs differently remains unknown. In order to help resolvethis critical question, we generated human iPSCs from a common fibroblast cell source using either the Yamanakafactors (OCT4, SOX2, KLF4 and cMYC) or the Thomson factors (OCT4, SOX2, NANOG and LIN28), and determinedtheir genome-wide DNA methylation profiles. In addition to shared DNA methylation aberrations present in all ouriPSCs, we identified Yamanaka-iPSC (Y-iPSC)-specific and Thomson-iPSC (T-iPSC)-specific recurrent ***, not only were the genomic locations of the aberrations different but also their types: reprogrammingwith Yamanaka factors mainly resulted in failure to demethylate CpGs, whereas reprogramming with Thomsonfactors mainly resulted in failure to methylate CpGs. Differences in the level of transcripts encoding DNMT3b andTET3 between Y-iPSCs and T-iPSCs may contribute partially to the distinct types of aberrations. Finally, de novoaberrantly methylated genes in Y-iPSCs were enriched for NANOG targets that are also aberrantly methylated insome cancers. Our study thus reveals that the choice of reprogramming factors influences the amount, location,and class of DNA methylation aberrations in iPSCs. These findings may provide clues into how to produce humaniPSCs with fewer DNA methylation abnormalities.

关键词： SOX2 programming Thomson

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：