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Antioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 702-707页

作者： farah ramdane Rym Essid Nadia Fares Dahmane El Ouassis Sana Aziz Mahfoud Hadj Mahammed Mohamed Didi Ould Hadj Ferid Limam Biogeochemistry Laboratory in Desert Environments Kasdi Merbah University Faculty of Natural and Life Sciences El Oued University Laboratory of Bioactive Substances Biotechnology Center of Borj-Cedria Technopole National Institute of Forest Research Protection Laboratory of Ecosystem in Arid and Semi Arid Zone Kasdi Merbah University

To study the phytochemical constituents and in vitro biological activities of hydromethanolic extract and fractions from Algerian Sahara Myrtus nivellei (M. nivellei) collected in Hoggar region and to identify the active fraction that can act as an alternative of commonly used antibiotics and as antileishmanial or antioxidant *** screening of M. nivellei aerial parts was realised according to the literature. Extract was firstly prepared by using aqueous methanol then fractionated with ethyl acetate and butanol solvents. Total phenolics, tannis and flavonoids, of the hydromethanolic extract and their fractions were determined by Folin-Ciocalteu method as gallic acid equivalents and by aluminium chloride as rutin equivalent respectively. Extract and fractions were tested for their antimicrobial and antiparasital activities against standard bacteria using agar diffusion method and two kinds of leishmania visceral and cutaneous. The antioxidant activities were realized using phosphomolybdenum, FRAP and DPPH *** phytochemical screening exhibited the presence of flavonoids, tannins, saponins, and alkaloids. The experimental results showed that plant extract and fractions were high in phenolic compounds and exhibited an important role as antioxidant, antimicrobial and had a moderate antileishmanial *** observations lead us toward more studies in this field, so that we can get more benefits from our local Algerian medicinal plants.

关键词： Phenolic compounds Flavonoids Antioxidant activity Antimicrobial activity Antileishmanial activity Myrtus nivellei

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