检索结果-南通市图书馆

Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

引用

Journal of Agricultural Science and Technology(A) 2018年第6期8卷 398-405页

作者： Ghizlane Sebbar Khalil Zro faouzi kichou Abdelkrim Fillali Maltouf Bouchra Belkadi Society of Veterinary Pharmaceutical and Biological Productions(Biopharma) B.P.4569RabatMorocco Laboratory of Microbiology and Molecular Biology Faculty of SciencesUniversity Mohammed V4 Ibn Battouta Ave.BP 1014 RP RabatMorocco Institute of Agronomy and Veterinary Medicine Hassan II B.P.6202RabatMorocco

Pasteurella species is considered the principal pathogen of the respiratory *** haemolytica and Pasteurella multocida were investigated and typed from nasal swabs and tissues taken from sheep,goat and ***,41 lung and 121 nasal swabs samples were collected from animals with respiratory diseases during 2015 to 2017 in six different regions in *** first,a screening of Pasteurella species using the real time PCR(RT-PCR)was carried out,then all isolated strains on agar blood were confirmed by PCR gel based assay specific for *** and *** was evaluated in mice and histopathological examination was done on some of lung *** results revealed that 34 samples of which 28(55%)from nasal swabs and six(38%)from lungs were positive for *** and nine samples of which seven(14%)from nasal swabs and two(13%)from lungs were positive on *** serogroup ***-two percent(72%)isolates were highly pathogenic to mice,which is in accordance with the results obtained by histopathology *** is the first report for widespread infections of Pasteurella(***&***)in ruminants in ***,measures including development of vaccines are highly required to mitigate the impact of the bacteria in animals.

关键词： Isolation identification PCR Mannheimia haemolytica Pasteurella multocida histopathology Morocco.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diagnosis of Clinical Cases of Infectious Bursal Disease Using a Modified Rapid Taq Man-MGB Real-Time RT-PCR Assay

引用

Journal of Agricultural Science and Technology(A) 2018年第4期8卷 230-238页

作者： Maryame Cheggag Khalil Zro Ghizlane Sebbar Naoufal Rahmatallah Mohamed Mouahid Mohammed EL Houadfi faouzi kichou Unit of Histology and Pathological Anatomy Department of Veterinary Pathology and Public HealthInstitute of Agronomy and Veterinary Medicine Hassan IIB.P.6202RabatMorocco Society of Veterinary Pharmaceutical and Biological Productions(Biopharma) B.P.4569RabatMorocco Veterinary Clinic Temara Morocco

Infectious bursal disease(IBD)is an important contagious viral infection of immune system of *** infection possesses a permanent threat to the profitability of poultry industry *** aim of this work was to modify the Taq Man-MGB real-time reverse transcription-polymerase chain reaction(rRT-PCR)in one step involving two fluorogenic Taq Man labeled probe and using this protocol for detection of infectious bursal disease virus(IBDV)collected from suspected cases distributed in different regions of the country during the period *** intralaboratory validation of modified method was realized for specificity,linearity,repeatability,sensitivity and *** allowed reducing the test running time by six *** method was applied on 102 pools of bursa of fabricius(BF)samples collected from affected broiler farms suspected to be infected by *** showing macroscopic lesions including muscle petechial hemorrhages,hypertrophy and hemorrhage of BF,were subjected to molecular analysis using modified protocol“Taq Man-MGB rRT-PCR”.The validation satisfied all criteria and the assay developed could be a useful tool for a very rapid diagnosis of IBDV and permit to detect and to discriminate in one-step very virulent(vv)from non-vv(classic and variant)IBDV *** of 84 IBDV positive samples,a prevalence of 39%for vv strains and 61%for classical strains was *** results indicate that despite the vaccination against IBDV,the vv form of this pathologie continues to cause serious problems for Moroccan broiler *** obtained results indicate the successfully detection of IBDV and differentiated all vvIBDV strains from non-vvIBDV strains;Avian infectious agent RNA viruses tested are negative,demonstrating great specificity of the *** results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of IBDV strains in samples of avian origin.

关键词： IBDV one-step rRT-PCR Taq Man-MGB macroscopic lesions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：