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Science China Life Sciences 2024年

作者： Hongyu Li Qingwen Ma Yan Xue Linlin Cai Liwen Bao Lei Hong Yitao zeng Shuzhen Huang Richard H.Finnell fanyi zeng Shanghai Institute of Medical Genetics Shanghai Children's Hospital Shanghai Jiao Tong University School of Medicine Department of Histo-Embryology Genetics and Developmental Biology Shanghai Jiao Tong University School of Medicine NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology Shanghai Key Laboratory of Embryo and Reproduction Engineering Center for Precision Environmental Health Baylor College of Medicine School of Pharmacy Macau University of Science and Technology

Autosomal recessive spinocerebellar ataxias（SCARs） are one of the most common neurodegenerative diseases characterized by progressive ataxia. Although SCARs are known to be caused by mutations in multiple genes, there are still many cases that go undiagnosed or are misdiagnosed. In this study, we presented a SCAR patient, and identified a probable novel pathogenic mutation（c.1A>G, p.M1V） in the AFG3L2 start codon. The proband's genotype included heterozygous mutations of the compound AFG3L2(p.[M1V]; [R632X]（c.[1A>G];[1894.C>T]）), which were inherited from the father（c.1A>G, p.M1V） and mother（c.1894C>T, p.R632X）. Functional studies performed on hiPSCs（human induced pluripotent stem cells） generated from the patients and HEK293T cells showed that the mutations impair mitochondrial function and the unbalanced expression of AFG3L2 mRNA and protein levels. Furthermore, this novel mutation resulted in the degradation of the protein and the reduction of the stability of the AFG3L2 protein, and MCU（mitochondrial calcium uniporter） complex mediated Ca2+overload.

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人工智能、劳资关系与劳动收入份额

当代经济科学 2024年第5期46卷 89-104页

作者：徐春华曾繁毅厦门大学经济学院/王亚南经济研究院福建361005 江西省投资集团有限公司江西330000

基于马克思主义政治经济学理论视角考察人工智能对劳动收入份额的作用机理,通过构造59个国家2001—2019年的平衡面板数据并采用面板向量自回归(PVAR)模型研究发现:(1)人工智能自身的技术属性不仅能够通过提高资本技术构成所产生的劳动... 详细信息

基于马克思主义政治经济学理论视角考察人工智能对劳动收入份额的作用机理,通过构造59个国家2001—2019年的平衡面板数据并采用面板向量自回归(PVAR)模型研究发现:(1)人工智能自身的技术属性不仅能够通过提高资本技术构成所产生的劳动力挤出效应降低劳动收入份额,还会通过其就业创造效应提升劳动收入份额;(2)人工智能作为使用价值生产的要素投入,将和普通物质资本的深化一同影响劳动收入份额变动,特别是在发展中国家或人工智能使用规模小的国家,人工智能使用增加会对劳动收入份额产生显著的负向影响;(3)人工智能的应用还将通过影响劳资关系变动而影响劳动收入份额变化,尤其是在发展中国家,人工智能的应用将抑制劳动收入份额下降。因此,在抢抓人工智能发展机遇的同时,还要客观认识人工智能发展对社会就业和劳动收入份额带来的冲击与机遇,尤其要通过加快形成新质生产力实现劳动收入份额的持续提升。

关键词：人工智能劳资关系劳动收入份额资本有机构成劳资议价能力新质生产力

新增未知攻击场景下的工业互联网恶意流量识别方法

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通信学报 2024年第6期45卷 75-86页

作者：曾凡一苘大鹏许晨韩帅王焕然周雪李欣纯杨武哈尔滨工程大学计算机科学与技术学院黑龙江哈尔滨150009

针对工业互联网中新增未知攻击所引发的流量数据分布偏移问题,提出了一种基于邻域过滤和稳定学习的恶意流量识别方法,旨在增强现有图神经网络模型在识别已知类恶意流量时的有效性和鲁棒性。该方法首先对流量数据进行图结构建模,捕获通... 详细信息

针对工业互联网中新增未知攻击所引发的流量数据分布偏移问题,提出了一种基于邻域过滤和稳定学习的恶意流量识别方法,旨在增强现有图神经网络模型在识别已知类恶意流量时的有效性和鲁棒性。该方法首先对流量数据进行图结构建模,捕获通信行为中的拓扑关系与交互模式;然后,基于有偏采样的邻域过滤机制划分流量子图,消除通信行为间的伪同质性;最后,应用图表示学习和稳定学习策略,结合自适应样本加权与协同损失优化方法,实现高维流量特征的统计独立性。2个基准数据集上的实验结果表明,相较对比方法,所提方法在新增未知攻击场景下的识别性能提升超过2.7%,展示了其在工业互联网环境下的高效性和实用性。

关键词：工业互联网恶意流量识别图神经网络邻域过滤稳定学习

Directed hepatic differentiation from embryonic stem cells

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Protein & Cell 2011年第3期2卷 180-188页

作者： Xuesong Chen fanyi zeng Laboratory of Developmental Biology Institute of Medical ScienceShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Institute of Medical Genetics Shanghai Jiao Tong University School of MedicineShanghai

The liver is the largest internal organ in mammals,and is important for the maintenance of normal physiological functions of other tissues and ***,cirrhosis,liver cancer and other chronic liver diseases are serious threats to human health,and these problems are compounded by a scarcity of liver donors for transplantation *** differentiation of embryonic stem cells to liver cells is a promising strategy for obtaining hepatocytes that can be used for cell *** vitro hepatocyte differentiation of embryonic stem cells requires a profound understanding of normal development during embryonic *** we provide a simple description of hepatogenesis in vivo and discuss directed differentiation of embryonic stem cells into hepatocytes in vitro.

关键词： stem cell differentiation liver development signaling pathway

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医学实验室试剂管理系统的构建与实践

实验室研究与探索 2024年第6期43卷 209-213页

作者：周密唐汇祥龙凌婕曾凡一上海市儿童医院上海交通大学医学院附属儿童医院上海医学遗传研究所上海200040 上海交通大学基础医学院组织胚胎学与遗传发育学系上海200025 国家卫健委胚胎分子生物学重点实验室上海市胚胎与生殖工程重点实验室上海200040

为实现医学实验试剂管理的信息化、网络化建设,以提升医学实验室管理质量和能力,设计了一套医学实验室试剂管理系统。该系统集成了基础设置、试剂入库、试剂出库、库存管理模块,通过钉钉平台及氚云开发工具实现低成本、定制化的管理功... 详细信息

为实现医学实验试剂管理的信息化、网络化建设,以提升医学实验室管理质量和能力,设计了一套医学实验室试剂管理系统。该系统集成了基础设置、试剂入库、试剂出库、库存管理模块,通过钉钉平台及氚云开发工具实现低成本、定制化的管理功能定制。该系统的构建遵循CNAS按照国际标准ISO15189:2022对医学实验室质量和能力认可的要求,实现了自助扫码出库、过期与低库存预警、多仓库管理和多项目独立管理等功能,兼具开发周期短、管理高效、便于维护等特点。实践表明,该系统可以满足医学实验室教学、科研工作中试剂管理的需求以及精准实验室标准化的管理要求,并在医学实验室试剂管理中起到了良好示范作用,为实验室其他应用场景提供有益借鉴。

关键词：试剂管理医学实验室管理系统

Integration-free Methods for Generating Induced Pluripotent Stem Cells

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Genomics, Proteomics & Bioinformatics 2013年第5期11卷 284-287页

作者： Yi-ye Zhou fanyi zeng Institute of Medical Science and Institute of Medical Genetics Shanghai Jiao Tong University School of Medicine

Induced pluripotent stem （iPS） cells by exogenous expression of four factors, Oct4, can be generated from mouse or human fibroblasts Sox2, Klf4 and c-Myc, and hold great potential for transplantation therapies and regenerative medicine. However, use of retroviral vectors during iPS cell generation has limited the techniques clinical application due to the potential risks resulting from genome integration of transgenes, including insertional mutations and altered differentiation potentials of the target cells, which may lead to pathologies such as tumorigenesis. Here we review recent progress in generating safer transgene-free or integration-free iPS cells, including the use of non-integrating vectors, excision of vectors after integration, DNA-free delivery of factors and chemical induction of pluripotency.

关键词： Induced pluripotent stem(iPS) cells Vector Transgene-free

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

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Science China(Life Sciences) 2014年第9期57卷 903-914页

作者： MOUSSA Mahmoud SHU Juan ZHANG XueHong zeng fanyi Shanghai Institute of Medical Genetics Shanghai Children’s Hospital Shanghai Jiao Tong University Institute of Medical Science School of Medicine Shanghai Jiao Tong University The Key Lab of Embryo Molecular Biology Ministry of Health China and Shanghai Lab of Embryo and Reproduction Engineering Shanghai Tao Tao Transgenic Corp. LTD Department of Bioengineering School of Life Sciences and Biotechnology Shanghai Jiao Tong University Department of Theriogenology Faculty of Veterinary Medicine Suez Canal University

Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades,emphasizing their importance in various assisted reproductive *** and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro *** progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos,including preservation of the reproductive potential of patients who may become infertile,establishment of cryopreserved oocyte banks,and transport of oocytes and embryos ***,the success rates are still far lower than those obtained with fresh oocytes and embryos,and there are still obstacles that need to be *** this review,we address the major obstacles in the development of effective cryopreservation *** knowledge may help to eliminate these hurdles by revealing which aspects need ***,this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species.

关键词： cryopreservation mammals oocytes embryos cryoinjuries

机械性血栓清除术与置管溶栓治疗急性下肢深静脉血栓的短中期疗效对比

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血管与腔内血管外科杂志 2024年第2期10卷 191-194,242页

作者：曾凡祎潘升权龙海灯殷世武崔建伟合肥市第二人民医院介入血管科安徽合肥230000

目的探讨机械性血栓清除术(PMT)与置管溶栓术(CDT)治疗急性下肢深静脉血栓(LEDVT)的短中期疗效。方法收集2019年4月至2021年4月于合肥市第二人民医院就诊的85例急性LEDVT患者的临床资料,按照治疗方法的不同将其分为PMT组(n=37)和CDT组... 详细信息

目的探讨机械性血栓清除术(PMT)与置管溶栓术(CDT)治疗急性下肢深静脉血栓(LEDVT)的短中期疗效。方法收集2019年4月至2021年4月于合肥市第二人民医院就诊的85例急性LEDVT患者的临床资料,按照治疗方法的不同将其分为PMT组(n=37)和CDT组(n=48)。比较两组患者大腿周径差、小腿周径差、尿激酶使用量、溶栓时间,术后出血、血红蛋白尿及血栓形成后综合征(PTS)发生情况。结果两组患者大腿周径差、小腿周径差比较,差异均无统计学意义(P>0.05)。PMT组患者溶栓时间明显短于CDT组患者,尿激酶使量明显小于CDT组患者,差异均有统计学意义(P0.05)。术后12个月,PMT组患者无PTS、轻度PTS比例均小于CDT组患者,差异均有统计学意义(P<0.05)。结论 PMT与CDT血栓清除率相似,PMT在避免短中期发生PTS方面更有优势,PMT溶栓时间短,溶栓药物使用剂量小,缩短了患者的住院时间。

关键词：机械性血栓清除术置管溶栓深静脉血栓疗效

Activation of proHGF by St14 induces mouse embryonic stem cell differentiation

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Protein & Cell 2016年第8期7卷 601-605页

作者： Yan, Xiaoshuang Xue, Yan Zhou, Yiye Cheng, Yan Yin, Shang Ma, Qingwen zeng, fanyi Shanghai Jiao Tong Univ Sch Med Inst Med Sci Shanghai 200025 Peoples R China Shanghai Jiao Tong Univ Shanghai Childrens Hosp Shanghai Inst Med Genet Shanghai 200040 Peoples R China Minist Hlth Key Lab Med Embryo Mol Biol Shanghai 200040 Peoples R China Shanghai Lab Embryo & Reprod Engn Shanghai 200040 Peoples R China

Embryonic stem cells （ESCs） are capable of unlimited selfrenewal and differentiation, thus generating remarkable interest in their potential therapeutic use in cell-based tissue engineering and regeneration. Stem cell based therapy has been considered as the most promising therapeutic strategy for a range of debilitating diseases （Reynolds and Lamba, 2014）.

关键词： Hepatocyte Growth Factor Pluripotent Gene Protease System Hepatocyte Growth Factor Activator Transmembrane Serine Protease

Effects of donor cells on in vitro development of cloned bovine embryos

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Journal of Genetics and Genomics 2008年第5期35卷 273-278页

作者： Jing Fu Pengfei Guan Leiwen Zhao Hua Li Shuzhen Huang fanyi zeng Yitao zeng Shanghai Institute of Medical Genetics Shanghai Children＇s Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200040 China

The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer （SCNT）. The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The oocytes were obtained through ovum pick up operation. After in vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer. The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastocyst as the parameters of SCNT efficiency. The donor cells from different individuals （04036, 06081, 06088, and 06129） had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate （P〈0.05）, and the rate was 62.3%, 37.0%, 35.1%, and 15.6%, respectively. There was no significant difference among the rate of fusion, cleaved and blastocyst in donor cells with different foreign genes （P〉0.05）. It was concluded that the genetic background of the donor cells could affect the efficiency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency.

关键词： donor cell bovine somatic nuclear transfer transgene