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MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

Science China(Life Sciences) 2021年第9期64卷 1533-1545页

作者： Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang fanjiang kong Meina Li Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 311401China Core Facility and Technical Service Center for SLSB School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Cereal&Oil Crop Institute Hebei Academy of Agricultural and Forestry SciencesShijiazhuang 050031China

Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding.

关键词： soybean male sterility ms1 kinesin cytokinesis hybrid breeding

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Development and validation of InDel markers for identification of QTL underlying flowering time in soybean

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The Crop Journal 2018年第2期6卷 126-135页

作者： Jialin Wang Lingping kong Kanchao Yu Fengge Zhang Xinyi Shi Yanping Wang Haiyang Nan Xiaohui Zhao Sijia Lu Dong Cao Xiaoming Li Chao Fang Feifei Wang Tong Su Shichen Li Xiaohui Yuan Baohui Liu fanjiang kong The Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina School of Life Sciences Guangzhou UniversityGuangzhou 510006GuangdongChina Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences Qiqihar 161006HeilongjiangChina Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157041HeilongjiangChina University of Chinese Academy of Sciences Beijing 100049China College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina The Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081HeilongjiangChina.

Soybean [Glycine max(L.) Merrill] is a major plant source of protein and oil. An accurate and well-saturated molecular linkage map is a prerequisite for forward genetic studies of gene function and for modern breeding for many useful agronomic traits. Next-generation sequence data available in public databases provides valuable information and offers new insights for rapid and efficient development of molecular markers. In this study, we attempted to show the feasibility and facility of using genomic resequencing data as raw material for identifying putative In Del markers. First, we identified 17,613 In Del sites among 56 soybean accessions and obtained 12,619 primer pairs. Second, we constructed a genetic map with a random subset of 2841 primer pairs and aligned 300 polymorphic markers with the 20 consensus linkage groups(LG). The total genetic distance was 2347.3 c M and the number of mapped markers per LG ranged from 10 to 23 with an average of 15 markers. The largest and smallest genetic distances between adjacent markers were 52.3 c M and 0.1 cM, respectively. Finally, we validated the genetic map constructed by newly developed In Del markers by QTL analysis of days to flowering(DTF) under different environments. One major QTL(qDTF4) and four minor QTL(qDTF20, qDTF13, qDTF12,and q DTF11) on 5 LGs were detected. These results demonstrate the utility of the In Del markers developed in this work for map-based cloning and molecular breeding in soybean.

关键词： Soybean Resequencing data InDel markers Genetic map QTL analysis

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FT5a interferes with the Dt1-AP1 feedback loop to control flowering time and shoot determinacy in soybean

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Journal of Integrative Plant Biology 2021年第6期63卷 1004-1020页

作者： Lin Yue Xiaoming Li Chao Fang Liyu Chen Hui Yang Jie Yang Zhonghui Chen Haiyang Nan Linnan Chen Yuhang Zhang Haiyang Li Xingliang Hou Zhicheng Dong James LWeller Jun Abe Baohui Liu fanjiang kong School of Life Sciences Innovative Center of Molecular Genetics and EvolutionGuangzhou UniversityGuangzhou 510006China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial Key Laboratory of Applied Botany South China Botanical Gardenthe Chinese Academy of SciencesGuangzhou 510650China University of the Chinese Academy of Sciences Beijing 100049China National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementNanjing Agricultural UniversityNanjing 210095China School of Natural Sciences University of TasmaniaHobartTasmania 7001Australia Research Faculty of Agriculture Hokkaido UniversitySapporo 060‐8589Japan The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and Agroecologythe Chinese Academy of SciencesHarbin 1500000Chin

Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1(TFL1), is a major controller of stem growth habit, but its underlying molecular mechanisms remain unclear.Here, we demonstrate that Dt1 affects node number and plant height, as well as flowering time,in soybean under long-day conditions. The b ZIP transcription factor FDc1 physically interacts with Dt1, and the FDc1-Dt1 complex directly represses the expression of APETALA1(AP1). We propose that FT5 a inhibits Dt1 activity via a competitive interaction with FDc1 and directly upregulates AP1. Moreover, AP1 represses Dt1 expression by directly binding to the Dt1 promoter, suggesting that AP1 and Dt1 form a suppressive regulatory feedback loop to determine the fate of the shoot apical meristem. These findings provide novel insights into the roles of Dt1 and FT5 a in controlling the stem growth habit and flowering time in soybean, which determine the adaptability and grain yield of this important crop.

关键词： AP1 Dt1 FDc1 flowering FT5a soybean stem growth habit

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Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time

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The Crop Journal 2021年第4期9卷 767-776页

作者： Zhaobo Li Qun Cheng Zhuoran Gan Zhihong Hou Yuhang Zhang Yongli Li Haiyang Li Haiyang Nan Cen Yang Linnan Chen Sijia Lu Wenqian Shi Liyu Chen Yanping Wang Chao Fang Liping kong Tong Su Shichen Li Kun Kou Lingshuang Wang fanjiang kong Baohui Liu Lidong Dong The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 130102HeilongjiangChina University of Chinese Academy of Sciences Beijing 100081China Innovation Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina Agronomy College of Heilongjiang Bayi Agricultural University Daqing 163319HeilongjiangChina National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm EnhancementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095JiangsuChina Mudanjiang Branch Heilongjiang Academy of Agricultural SciencesMudanjiang 157000HeilongjiangChina

Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of soybean adaptation to diverse latitudes is still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2(LNK2) homeologs of Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs were designed for editing the four LNK2 genes. A transgene-free homozygous quadruple mutant of the LNK2 genes was developed using the CRISPR(clustered regularly interspaced short palindromic repeats)/Cas9(CRISPR-associated protein 9). Under long-day(LD) conditions, the quadruple mutant flowered significantly earlier than the wild-type(WT). Quantitative real-time PCR(q RT-PCR)revealed that transcript levels of LNK2 were significantly lower in the quadruple mutant than in the WT under LD conditions. LNK2 promoted the expression of the legume-specific E1 gene and repressed the expression of FT2 a. Genetic markers were developed to identify LNK2 mutants for soybean breeding.These results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four LNK2 genes shortens flowering time in soybean. Our findings identify novel components in flowering-time control in soybean and may be beneficial for further soybean breeding in high-latitude environments.

关键词： Soybean LNK2 CRISPR/Cas9 Genome editing Flowering time

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Modulation of nitrate-induced phosphate response by the MYB transcription factor RLI1/HINGE1 in the nucleus

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Molecular Plant 2021年第3期14卷 517-529页

作者： Zhihua Zhang Zhao Li Wei Wang Zhimin Jiang Liping Guo Xiaohan Wang Yangwen Qian Xiahe Huang Yongqiang Liu Xiujie Liu Yahong Liu Aifu Li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva Legong Li fanjiang kong Baohui Liu Yingchun Wang Bin Hu Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina School of Life Sciences Guangzhou University.GuangzhouChina College of Plant Science Jilin UniversityChangchunChina Biogle Genome Editing Center ChangzhouChina State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina College of Advanced Agricultural Science University of Chinese Academy of SciencesBeijingChina Botanical Institute Cluster of Excellence on Plant SciencesUniversity of CologneCologneGermany College of Life Sciences Capital Normal UniversityBeijingChina

The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in rice.In this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as RLI1),a MYB-transcription factor closely related to PHR2.RLI1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced genes.More importantly,RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate response.Therefore,RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关键词： rice NIPR RLI1/HINGE1 PHR2 SPXs nucleus

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Molecular mechanism underlying photoperiod regulated flowering in soybean

Molecular mechanism underlying photoperiod regulated floweri...

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第十四届全国植物基因组学大会

作者： fanjiang kong Abe Jun Baohui Liu Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of Sciences Research Faculty of Agriculture Hokkaido University

Flowering is one of the most important processes involved in crop adaptation and productivity.A number of major genes and quantitative trait loci(QTLs) for flowering have been reported in soybean(Glycine max).These ge... 详细信息

关键词： soybean QTL flowering maturity yield adaptation

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植物光信号转导研究领域近十年重要研究进展

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植物生理学报 2024年第3期60卷 399-429页

作者：林荣呈刘宏涛李继刚孔凡江刘斌王海洋杨洪全钟上威朱丹萌淮俊玲李洪刘双荣王璠王文秀茅志磊邓兴旺中国科学院植物研究所光生物学重点实验室北京100093 中国科学院分子植物科学卓越创新中心上海200032 中国农业大学生物学院植物抗逆高效全国重点实验室北京100193 广州大学生命科学学院分子遗传与进化创新研究中心广东省植物适应性与分子设计重点实验室广州510006 中国农业科学院作物科学研究所作物基因与分子设计中心作物基因资源与育种全国重点实验室北京100081 华南农业大学生命科学学院亚热带农业生物资源保护与利用国家重点实验室广州510642 上海师范大学生命科学学院上海市植物分子科学重点实验室上海200234 北京大学生命科学学院蛋白质与植物基因研究国家重点实验室北京100871 北京大学现代农业研究院潍坊现代农业山东省实验室小麦育种全国重点实验室山东潍坊261325 北京大学现代农学院小麦育种全国重点实验室北京100871

光是影响植物生长和发育的一种环境信号,在植物的整个生命周期中发挥关键的调控功能。光信号转导的研究已有一百多年的历史,一直都是植物生物学研究领域的一个热点。本文从光敏色素、隐花素、紫外B受体等3种信号转导途径、光信号转导的... 详细信息

光是影响植物生长和发育的一种环境信号,在植物的整个生命周期中发挥关键的调控功能。光信号转导的研究已有一百多年的历史,一直都是植物生物学研究领域的一个热点。本文从光敏色素、隐花素、紫外B受体等3种信号转导途径、光信号转导的共性调控机制、光信号与内源激素信号和其他环境信号的互作、以及作物光形态建成等方面,对植物光信号转导研究领域近十年来的重要进展进行了概述。

关键词：光受体光信号转导信号互作

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Molecular mechanism of low latitude adaptation in soybean

Molecular mechanism of low latitude adaptation in soybean

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2018全国植物生物学大会

作者： Sijia Lu Xiaohui Zhao Yilong Hu Shulin Liu Haiyang Nan Xiaoming Li Chao Fang Dong Cao Xinyi Shi Lingping kong Tong Su Fengge Zhang Shichen Li Zheng Wang Xiaohui Yuan Elroy R.Cober James L.Weller Baohui Liu Xingliang Hou Zhixi Tian fanjiang kong Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of Sciences School of Life Sciences Guangzhou University Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences Eastern Cereal and Oilseed Research Centre Agricultureand Agri-Food Canada Central Experimental Farm School of Plant Science University of Tasmania

Soybean is a major legume crop that has its origins in temperate regions,and photoperiod responsiveness is a major factor in its latitudinal adaptation.Varieties from temperate regions introduced to lower latitudes typically flower and mature very early,and as a consequence grain yield is extremely low.However,introduction of the long juvenile(LJ) trait,which extends the vegetative phase and improves yield under short day conditions,has largely overcome this constraint and enabled a major expansion of soybean cultivation in tropical regions.Here we report map-based cloning and functional characterization of J,the major classical locus conferring the LJ trait.We show that J gene is the soybean orthologue of Arabidopsis ELF3,and identify variation at J as the molecular basis for the adaptation and enhancement of grain yield in tropics.The effects of J depend genetically on E1,a key legume-specific repressor of flowering,and the J protein associates physically with the E1 promoter to down-regulate its transcription,relieving the E1-dependent repression of two important FT genes that are targets of E1,and promoting flowering under short day(SD) conditions.Our findings identify an important new component in the mechanism for flowering time control in soybean,and give new insight into an adaptation of major significance for soybean production in tropical regions.

关键词： soybean photoperiod adaptation flowering grain yield tropics

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露天矿车载安防设备在线监测系统

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工矿自动化 2021年第S2期47卷 111-116页

作者：刘鹏孔凡江神华准能集团有限责任公司内蒙古鄂尔多斯010300 北京中矿华沃科技股份有限公司北京100083

露天矿车辆、设备数量繁多,在长期的生产作业中,车载安防设备发生故障的情况十分普遍,现有故障识别方法难以及时地对设备故障进行宏观的统计和分析。针对该问题,设计了露天矿车载安防在线监测系统,该系统可将露天矿中所有车辆安防设备... 详细信息

露天矿车辆、设备数量繁多,在长期的生产作业中,车载安防设备发生故障的情况十分普遍,现有故障识别方法难以及时地对设备故障进行宏观的统计和分析。针对该问题,设计了露天矿车载安防在线监测系统,该系统可将露天矿中所有车辆安防设备的状态进行汇总统计,通过车辆上搭载的Linux5.0主机自动识别故障设备,并可将设备故障信息通过4G/5G网络上传至服务器,管理人员在后台通过软件可查看全部车辆安防设备的运行状态,可以查看哪些车辆的安防设备发生故障,同时该系统也可以查看各个时间段设备的故障记录,便于工作人员通过大数据分析故障原因,发现存在的问题。该系统对露天矿企业的生产调度、设备故障分析、设备健康状态及设备改进升级起到了重要的指导作用。

关键词：露天矿运输车辆设备故障识别车载安防在线监测

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甜菜品种(系)的ISSR标记数字指纹图谱构建及聚类分析(英文)

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农业工程学报 2012年第S2期28卷 280-284页

作者：刘巧红程大友杨林罗成飞孔凡江吴玉梅哈尔滨工业大学食品科学与工程学院哈尔滨150090 中国农业科学院东北地理生态研究所哈尔滨130012 中国农业科学院甜菜研究所哈尔滨150080

本文针对来源于荷兰的4个引进甜菜品种和国内的6个甜菜品系(其中2个为一年生野生甜菜)进行了ISSR指纹图谱构建和聚类分析研究。筛选出稳定性高且多态性好的6个引物用于试验。利用筛选的6条引物ISSR-PCR共扩增出51个条带,其中多态性条带... 详细信息

本文针对来源于荷兰的4个引进甜菜品种和国内的6个甜菜品系(其中2个为一年生野生甜菜)进行了ISSR指纹图谱构建和聚类分析研究。筛选出稳定性高且多态性好的6个引物用于试验。利用筛选的6条引物ISSR-PCR共扩增出51个条带,其中多态性条带百分率为86.3%.利用该6条引物ISSR-PCR建立的指纹图谱能将试验中的全部甜菜品种都鉴定区分开。只利用2条引物L1和UBC846扩增的8个多态性条带构建了10个甜菜品种(系)的数字指纹识别码,该数字指纹图谱能完全区分10个甜菜品种(系),结果显示ISSR指纹图谱能非常有效的鉴定不同的甜菜品种。利用生物软件NTSYS-pc针对10个试验甜菜品种(系)的ISSR扩增条带进行遗传相似性聚类分析,结果显示10个甜菜品种(系)的相似系数为0.43与0.83之间,平均为0.62。利用非加权组平均法(UPGMA)进行聚类分析,结果显示10个甜菜品种(系)聚类为2个组和3个亚组。UPGMA聚类分析能清楚的显示10个甜菜群体间的遗传关系并且聚类结果与10个甜菜群体的特性一致,说明ISSR标记能用于甜菜不同群体间遗传距离的评估。

关键词：主成分分析农产品品质控制甜菜 ISSR标记指纹图谱遗传相似性

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