检索结果-南通市图书馆

D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-βsignaling pathway

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2022年第5期11卷 1215-1221页

作者： Chong wang fangting wang Yanbo wang Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

D-tryptophan is a special kind of nonprotein amino acid showing multiple physiological functions,but the detailed mechanisms are not fully revealed,impairing its further development and *** work was to investigate D-tryptophan physiological function and demonstrate the underlying mechanisms.D-tryptophan suppressed HaCaT cell proliferation but increased cell ***,D-tryptophan decreased E-cadherin and increased Snail,Twist,and Slug expression,resulting in the development of an epithelial-mesenchymal transitions(EMT)***,D-tryptophan promoted the expression of transforming growth factor-β(TGF-β)1,and Smad4 knockout damages D-tryptophan’s *** results indicated that D-tryptophan stimulated HaCaT cells to produce TGF-β1 and thus activated the TGF-β/Samd pathway,resulting in the triggering of *** study revealed the molecular mechanisms of D-tryptophan activity,provided D-tryptophan as a potential approach for cancer treatment,wound healing,organ development and other relevant applications.

关键词： D-tryptophan Epithelial-mesenchymal transitions Smad4 Transforming growth factor-β

同方期刊数据库

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2023年第3期12卷 737-744页

作者： Yanbo wang Huan Li Jinru Zhou fangting wang Yifan Qian Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic *** this work,a polydopamine(PDA)-based fluorescent aptasensor was developed to detect arginine kinase(AK),one of the major allergens in *** aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite(FAM)to build a fluorescence resonance energy transfer(FRET)system with PDA *** glycol(PEG)was employed to construct an antifouling surface for the aptasensor to eliminate food matrix *** the presence of AK,the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA *** aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection(LOD)of 0.298μg/*** proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products,which indicated its high potential in tracing the presence of AK in complex aquatic products.

关键词： Food allergy Aptasensor Antifouling Polydopamine FRET effect

同方期刊数据库

Elucidate long-term changes of ozone in Shanghai based on an integrated machine learning method

在线全文

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2023年第11期17卷 223-238页

作者： Jin Xue fangting wang Kun Zhang Hehe Zhai Dan Jin Yusen Duan Elly Yaluk Yangjun wang Ling Huang Yuewu Li Thomas Lei Qingyan Fu Joshua S.Fu Li Li School of Environmental and Chemical Engineering Shanghai UniversityShanghai 200444China Key Laboratory of Organic Compound Pollution Control Engineering(MOE) Shanghai UniversityShanghai 200444China Shanghai Environmental Monitoring Center Shanghai 200235China Institute of Science and Environment University of Saint JosephMacao 999078China Department of Civil and Environmental Engineering University of TennesseeKnoxvilleTN 37996USA

Surface ozone(O_(3))is influenced by regional background and local photochemical formation under favorable meteorological *** the contribution of these factors to changes in O_(3)is crucial to address the issue of O_(3)*** this study,we propose a novel integrated method that combines random forest,principal component analysis,and Shapley additive explanations to distinguish observed O_(3)into meteorologically affected ozone(O_(3_MET)),chemically formed from local emissions(O_(3_LC)),and regional background ozone(O_(3_RBG)).Applied to three typical stations in Shanghai during the warm season from 2013 to 2021,the results indicate that O_(3_RBG)in Shanghai was 48.8±0.3 ppb,accounting for 79.6%–89.4%at different sites,with an overall declining trend of 0.018 ppb/yr.O_(3_LC)at urban and regional sites ranged from 5.9–9.0 ppb and 8.9–14.6 ppb,respectively,which were significantly higher than the contributions of 2.5–7.4 ppb at an upwind background site.O_(3_MET)can be categorized into those affecting O_(3)photochemical generation and those changing O_(3)dispersion conditions,with absolute contributions to O_(3)ranging from 13.4–19.0 ppb and 13.1–13.7 ppb,*** found that the O_(3)rebound in 2017,compared to 2013,was primarily influenced by unfavorable O_(3)dispersion conditions and unbalanced emission reductions;while the O_(3)decline in 2021,compared to 2017,was primarily influenced by overall favorable meteorological conditions and further emissions *** findings highlight the challenge of understanding O_(3)change due to meteorology and regional background,emphasizing the need for systematic interpretation of the different components of O_(3).

关键词： Ozone Integrated method Machine learning

同方期刊数据库

Genome Mining and Biosynthesis Study of a Type B Linaridin Reveals a Highly Versatileα-N-Methyltransferase

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2021年第3期3卷 1049-1057页

作者： fangting wang Wanqing Wei Junfeng Zhao Tianlu Mo Xin wang Xuedong Huang Suze Ma Shu wang Zixin Deng Wei Ding Yong Liang Qi Zhang Department of Chemistry Fudan UniversityShanghai 200433 State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic MaterialsChemistry and Biomedicine Innovation CenterSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023 State Key Laboratory of Microbial Metabolism School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai 200240

Linaridins are a small but growing family of natural products belonging to the ribosomally synthesized and post-translationally modified peptide(RiPP)*** this study,a genome mining approach led to the identification of a novel linaridin,mononaridin(MON),from Streptomyces ***-frame deletion genetic knockout studies showed that,in addition to many genes essential for MON biosynthesis,monM encodes an S-adenosyl methionine(SAM)-dependentα-N-methyltransferase that is responsible for installing two methyl groups in the MON *** SAM,MonM also accepts ethyl-SAM and allyl-SAM,in which the methyl of SAM is replaced by an ethyl and an allyl,*** showed that ethyl-SAM and allyl-SAM have distinct reactivities in MonM catalysis,and this observation was further investigated in detail by density functional theory(DFT)***,MonM acts efficiently on nisin,a prototypic lantibiotic that is structurally very different from the native substrate,and the ability of MonM to transfer an allyl group to the nisin N-terminus allowed production of a fluorescently labeled nisin,which can be further used in microscopic cell *** studies provide new insights into linaridin biosynthesis and demonstrate the potential of linaridin methyltransferases in bioengineering applications.

关键词： substrate promiscuity SAM analogue dehydrobutyrine biosynthesis lanthipeptide

枯草芽孢杆菌对冰鲜罗非鱼肠道菌群的调节及保鲜作用

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国食品学报 2020年第9期20卷 173-179页

作者：张雯王芳婷倪莉福州大学食品科学技术研究所福建省食品生物技术创新工程技术研究中心福州350108 福建省新闽科生物科技开发有限公司福州350108

采用一株枯草芽孢杆菌(BS08)作为饲料添加剂喂养罗非鱼,研究其对冰鲜罗非鱼肠道菌群的影响,尤其是对鱼体腐败菌的粘附、定植和滋生的影响,并研究枯草芽孢杆菌延缓冰鲜罗非鱼腐败的作用。通过饲料中添加枯草芽孢杆菌,水体中添加腐败菌(... 详细信息

采用一株枯草芽孢杆菌(BS08)作为饲料添加剂喂养罗非鱼,研究其对冰鲜罗非鱼肠道菌群的影响,尤其是对鱼体腐败菌的粘附、定植和滋生的影响,并研究枯草芽孢杆菌延缓冰鲜罗非鱼腐败的作用。通过饲料中添加枯草芽孢杆菌,水体中添加腐败菌(希瓦氏菌和假单胞菌)的方式分组喂养罗非鱼后,对罗非鱼进行冰鲜贮藏,通过高通量测序确定枯草芽孢杆菌和腐败菌对冰鲜过程中罗非鱼肠道菌群的影响,并以TVB-N和K值为腐败指标,研究冰鲜鱼腐败的程度。结果表明:枯草芽孢杆菌对罗非鱼的肠道菌群的变化具有阻遏作用,使菌群多样性维持在一个相对稳定的水平。希瓦氏菌在肠道内定植程度大于假单胞菌,枯草芽孢杆菌可定植在鱼肠道内,并抑制希瓦氏菌在鱼肠道内定植,最终显著遏制希瓦氏菌在肠道中的优势富集。以TVB-N和K值为指标判断鱼的鲜度,添加枯草芽孢杆菌可显著延缓冰鲜鱼的腐败程度。

关键词：罗非鱼肠道菌群鲜度枯草芽孢杆菌希瓦氏菌假单胞菌

在线全文

学校读者我要写书评

暂无评论

大黄鱼源腐败菌的腐败能力与粘附性研究

中国食品学报 2019年第1期19卷 117-124页

作者：张雯卞丹王芳婷张晨倪莉福州大学食品科学技术研究所福建省食品生物技术创新工程技术研究中心福州350108

以从大黄鱼中分离得到的腐败菌为研究对象,从菌体粘附性这一角度解释希瓦氏菌成为海水鱼的优势腐败菌的原因。以细菌总数、TVB-N、TMA和K值为腐败指标,测定分离纯化自冰鲜大黄鱼体的3株希瓦氏菌(MA1-5,MA1-7,MA1-13)和3株假单胞菌(R3-1,... 详细信息

以从大黄鱼中分离得到的腐败菌为研究对象,从菌体粘附性这一角度解释希瓦氏菌成为海水鱼的优势腐败菌的原因。以细菌总数、TVB-N、TMA和K值为腐败指标,测定分离纯化自冰鲜大黄鱼体的3株希瓦氏菌(MA1-5,MA1-7,MA1-13)和3株假单胞菌(R3-1,R3-2,R3-5)的腐败能力,测定此6株腐败菌对大黄鱼体表、鱼鳃和鱼肠黏液的粘附能力,建立腐败能力与粘附能力之间的相关性。结果表明,希瓦氏菌的致腐性强于假单胞菌:希瓦氏菌的细菌总数增速显著高于假单胞菌,产TMA能力显著强于假单胞菌,K值变化快,TVB-N含量与冷藏时间呈指数增长趋势;而假单胞菌产TVB-N含量与冷藏时间呈正比趋势。希瓦氏菌对鱼体各部位黏液的粘附能力强于假单胞菌:希瓦氏菌对鱼鳃和鱼肠的粘附性高于体表,在高盐条件下时的粘附能力随氯化钠含量的升高而增强,而假单胞菌则对体表的粘附性较高。腐败能力与粘附力相关性分析表明,腐败菌的腐败能力和粘附能力呈正相关,尤其腐败菌对肠黏液的粘附能力与腐败能力呈极显著的正相关性。结果表明,希瓦氏菌在高盐浓度下对鱼鳃和鱼肠的高粘附力是其成为海水鱼优势腐败菌的原因之一。

关键词：大黄鱼希瓦氏菌假单胞菌腐败能力粘附优势腐败菌

长江流域武汉段典型湖泊中微塑料的赋存特征及生态风险评估

在线全文

学校读者我要写书评

暂无评论

环境工程学报 2023年第12期17卷 3953-3959页

作者：王芳婷包科齐信中国地质调查局武汉地质调查中心武汉430205 长江水利委员会河湖保护与建设运行安全中心武汉430010

塑料污染在环境中具有普遍性,对生态系统具有潜在的风险性,为新兴的全球性环境问题。武汉境内江河纵横、百湖密布,是全球同纬度地区和长江中下游湖泊型湿地的典型代表。调查了武汉湖泊表层水体中微塑料的分布特征,并采用生态风险指数(RI... 详细信息

塑料污染在环境中具有普遍性,对生态系统具有潜在的风险性,为新兴的全球性环境问题。武汉境内江河纵横、百湖密布,是全球同纬度地区和长江中下游湖泊型湿地的典型代表。调查了武汉湖泊表层水体中微塑料的分布特征,并采用生态风险指数(RI)评估了微塑料的生态风险。结果表明,微塑料丰度为2 000~7 733 items·m^(-3),远城区湖泊表层水体微塑料丰度通常高于城乡结合区湖泊,中心城区微塑料丰度具有显著差异。湖泊中微塑料以纤维状为主,其次是碎片状,大小以<1 mm的小颗粒为主,主要颜色为透明和蓝色,主要成分为聚乙烯(PE)、聚丙烯(PP)和聚对苯二甲酸乙二醇酯(PET)。武汉市典型湖泊表层水体中MPs生态风险指数为601.5~8 954,均属于危险或高危等级,且PE、PP和PET的生态风险指数普遍较高。该研究结果可为城市湖泊中微塑料污染治理提供参考。

关键词：微塑料空间分布湖泊生态风险评估武汉

在线全文

学校读者我要写书评

暂无评论

基于双目视觉多曝光的粒子轨迹测速方法研究

光学学报 2021年第12期41卷 154-162页

作者：周骛王芳婷王宵宵唐欣然蔡小舒上海理工大学能源与动力工程学院上海200093 上海市动力工程多相流动与传热重点实验室上海200093

提出双目视觉结合单帧多曝光技术的粒子轨迹测速(PSV)方法,通过长短曝光组合判断速度方向,采用灰度拟合法识别轨迹特征,并采用轨迹中心点进行速度计算以提高速度测量的准确性。该方法采用最邻近点匹配及倾斜角约束对同一颗粒的多段轨迹... 详细信息

提出双目视觉结合单帧多曝光技术的粒子轨迹测速(PSV)方法,通过长短曝光组合判断速度方向,采用灰度拟合法识别轨迹特征,并采用轨迹中心点进行速度计算以提高速度测量的准确性。该方法采用最邻近点匹配及倾斜角约束对同一颗粒的多段轨迹进行匹配与排序,利用极线约束对轨迹特征点进行双目匹配,对三维重建后的特征点进行B样条拟合以计算粒子的三维速度,而多曝光粒子轨迹法可以降低双目匹配的难度。形成了双目多曝光PSV的实验系统及相应图像处理流程。采用发光小孔进行了不同三维速度下的验证实验,分析了不同轨迹特征点选择对速度测量结果的影响。结果表明,利用轨迹中点计算速度,得到的平均相对误差小于4.2%,同时其标准偏差较小,故采用轨迹中点相较于采用轨迹端点的速度计算方法更优。并对一射流流场进行了实测,结果表明此方法能够可靠地用于三维流场测量。

关键词：机器视觉双目视觉粒子轨迹测速多曝光灰度拟合三维速度

在线全文

学校读者我要写书评

暂无评论

基于离焦成像的粒子轨迹测速

光学学报 2021年第19期41卷 160-166页

作者：王宵宵周骛王芳婷唐欣然蔡小舒上海理工大学能源与动力工程学院上海200093 上海市动力工程多相流动与传热重点实验室上海200093

提出将离焦成像与粒子轨迹测速相结合,分别针对直线型和曲线型粒子轨迹,构建了离焦特征参数σ的识别算法,形成了用于三维速度测量的离焦粒子轨迹测速(DPSV)方法。该方法基于粒子图像离焦特征参数σ沿运动轨迹线的线性变化假设,通过拟合... 详细信息

提出将离焦成像与粒子轨迹测速相结合,分别针对直线型和曲线型粒子轨迹,构建了离焦特征参数σ的识别算法,形成了用于三维速度测量的离焦粒子轨迹测速(DPSV)方法。该方法基于粒子图像离焦特征参数σ沿运动轨迹线的线性变化假设,通过拟合粒子图像的灰度分布来识别σ,从而获得粒子深度信息。在直线轨迹拟合的基础上,进一步采用圆弧拟合处理湍流实验中常见的弯曲轨迹图像。形成了相应的图像处理流程,并采用仿真图像验证了参数识别的准确性,在本文实验装置的噪声水平下相对误差为9.4%左右。采用LED光源和5μm孔径光阑组成的发光点进行模拟实验,验证了深度位置z与σ的线性关系。最后,将DPSV技术应用于射流流场,得到了三维速度分布。

关键词：测量粒子轨迹测速弯曲轨迹离焦成像三维速度灰度分布