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The Crop Journal 2024年第2期12卷 456-469页

作者： Jingli Ding Chenchen Ji Lu Yu Chuang Wang Guangda Ding Sheliang Wang Lei Shi fangsen xu Hongmei Cai Research Center of Microelement Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070HubeiChina National Key Laboratory of Crop Genetics and Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been identified.We isolated a novel R2R3-MYB transcription factor,OsMYB84,and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2 and OsHMA expression.OsMYB84 was highly expressed in roots and anthers and induced by Cu.Overexpression of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice,facilitated Cu distribution into grain and increased grain yield.In contrast,mutation of OsMYB84 reduced Cu concentration in xylem sap.OsMYB84 bound to the promoter region of OsCOPT2 and OsHMA5 and upregulated their expression.OsCOPT2 mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.

关键词： OsMYB84 OsCOPT2 OsHMA5 Copper Rice

Brassinosteroid signaling regulates phosphate starvation-induced malate secretion in plants

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Journal of Integrative Plant Biology 2023年第5期65卷 1099-1112页

作者： Tongtong Liu Suren Deng Cheng Zhang xu Yang Lei Shi fangsen xu Sheliang Wang Chuang Wang Microelement Research Center College of Resources&EnvironmentHuazhong Agricultural UniversityWuhan430070 China Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of AgricultureHuazhong Agricultural UniversityWuhan430070 China

Inorganic phosphate(Pi)is often limited in soils due to precipitation with iron(Fe)and aluminum(Al).To scavenge heterogeneously distributed phosphorus(P)resources,plants have evolved a local Pi signaling pathway that induces malate secretion to solubilize the occluded Fe-P or Al-P oxides.In this study,we show that Pi limitation impaired brassinosteroid signaling and downregulated BRASSINAZOLE-RESISTANT 1(BZR1)expression in Arabidopsis thaliana.Exogenous 2,4-epibrassinolide treatment or constitutive activation of BZR1(in the bzr1-D mutant)significantly reduced primary root growth inhibition under Pi-starvation conditions by downregulating ALUMINUM-ACTIVATED MALATE TRANSPORTER 1(ALMT1)expression and malate secretion.Furthermore,At BZR1 competitively suppressed the activator effect of SENSITIVITY TO PROTON RHIZOTOXICITY 1(STOP1)on ALMT1 expression and malate secretion in Nicotiana benthamiana leaves and Arabidopsis.The ratio of nuclear-localized STOP1 and BZR1 determined ALMT1 expression and malate secretion in Arabidopsis.In addition,BZR1-inhibited malate secretion is conserved in rice(Oryza sativa).Our findings provide insight into plant mechanisms for optimizing the secretion of malate,an important carbon resource,to adapt to Pi-deficiency stress.

关键词： ALMT1 brassinosteroid signaling bzr1 malate phosphate deficiency STOP1

Induction of jasmonic acid biosynthetic genes inhibits Arabidopsis growth in response to low boron

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Journal of Integrative Plant Biology 2021年第5期63卷 937-948页

作者： Yupu Huang Sheliang Wang Chuang Wang Guangda Ding Hongmei Cai Lei Shi fangsen xu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Microelement Research Center College of Resources&EnvironmentHuazhong Agricultural UniversityWuhan 430070China

The essential micronutrient boron(B) has key roles in cell wall integrity and B deficiency inhibits plant growth. The role of jasmonic acid(JA) in plant growth inhibition under B deficiency remains unclear. Here,we report that low B elevates JA biosynthesis in Arabidopsis thaliana by inducing the expression of JA biosynthesis genes. Treatment with JA inhibited plant growth and, a JA biosynthesis inhibitor enhanced plant growth, indicating that the JA induced by B deficiency affects plant growth. Furthermore,examination of the JA signaling mutants jasmonate resistant1, coronatine insensitive1-2, and myc2 showed that JA signaling negatively regulates plant growth under B deficiency. We identified a low-B responsive transcription factor, ERF018, and used yeast one-hybrid assays and transient activation assays in Nicotiana benthamiana leaf cells to demonstrate that ERF018 activates the expression of JA biosynthesis genes. ERF018 overexpression(OE)lines displayed stunted growth and up-regulation of JA biosynthesis genes under normal B conditions,compared to Col-0 and the difference between ERF018 OE lines and Col-0 diminished under low B.These results suggest that ERF018 enhances JA biosynthesis and thus negatively regulates plant growth. Taken together, our results highlight the importance of JA in the effect of low B on plant growth.

关键词： Arabidopsis thaliana ERF018 low B jasmonic acid biosynthesis jasmonic acid signaling plant growth

Genotypic variation in phosphorus acquisition from sparingly soluble P sources is related to root morphology and root exudates in Brassica napus

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Science China(Life Sciences) 2011年第12期54卷 1134-1142页

作者： ZHANG HaiWei HUANG Yu YE XiangSheng xu fangsen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China Key Laboratory of Subtropical Agriculture and Environment Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China

Genotypic variations in the adaptive response to low-phosphorus(P) stress and P-uptake efficiency have been widely reported in many crops.We conducted a pot experiment to evaluate the P-acquisition ability of two rapeseed(Brassica napus) genotypes supplied with two sparingly soluble sources of P,Al-P and Fe-P.Then,the root morphology,proton concentrations,and carboxylate content were investigated in a solution experiment to examine the genotypic difference in P-acquisition efficiency.Both genotypes produced greater biomass and accumulated more P when supplied with Al-P than when supplied with Fe-P.The P-efficient genotype 102 showed a significantly greater ability to deplete sparingly soluble P from the rhizosphere soil because of its greater biomass and higher P uptake compared with those of the P-inefficient genotype 105.In the solution experiment,the P-efficient genotype under low-P conditions developed dominant root morphological traits,and it showed more intensive rhizosphere acidification because of greater H+ efflux,higher H+-ATPase activity,and greater exudation of carboxylates than the P-inefficient genotype.Thus,a combination of morphological and physiological mechanisms contributed to the genotypic variation in the utilization of different sparingly soluble P sources in B.napus.

关键词：基因型差异甘蓝型油菜根系形态性状难溶性磷根系分泌物磷源收购 ATP酶活性

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遮阴栽培对雪茄茄衣烟叶叶面均匀性的影响

中国农业科技导报 2024年第2期26卷 216-225页

作者：郭昊谭荣雷杨锦鹏余君黄文昌杨久红乔保明杨瑞玮徐芳森杨春雷丁广大华中农业大学资源与环境学院微量元素研究中心武汉430070 湖北省烟草科学研究院武汉430030 中国烟草总公司湖北省公司烟叶管理处武汉430030

为探究遮阴栽培对雪茄茄衣品种叶面均匀性的影响,以雪茄茄衣品种‘楚雪26号’(CX-26)为研究对象,在大田试验条件下进行不同程度的遮阴处理,分析其成熟期上部叶和中部叶的叶面厚度、叶面平展度、叶片密度、主支脉粗细、主支脉占叶片干物... 详细信息

为探究遮阴栽培对雪茄茄衣品种叶面均匀性的影响,以雪茄茄衣品种‘楚雪26号’(CX-26)为研究对象,在大田试验条件下进行不同程度的遮阴处理,分析其成熟期上部叶和中部叶的叶面厚度、叶面平展度、叶片密度、主支脉粗细、主支脉占叶片干物质量比例以及SPAD值等的差异。结果表明,随着遮阴程度的增加,上部叶的叶面厚度、叶片密度以及叶片SPAD值均逐渐下降;当透光率为70%时,叶面厚度、叶片密度与不遮阴处理相比分别下降35.5%和33.3%,而中部叶中各性状则逐渐上升。遮阴栽培显著增加上部叶的叶面平展度,但降低了中部叶的叶面平展度;在透光率为70%时,上部叶的叶面平展度达到最大,为0.98;遮阴栽培增加上部叶主支脉占叶片干物质量比例,对中部叶主支脉占叶片干物质量比例的影响随遮阴程度的增加呈下降趋势,但差异不显著。遮阴栽培对中部叶主支脉的粗细程度无影响,但使上部叶的主支脉变细。以上研究结果为国产雪茄茄衣烟叶遮阴栽培技术提供理论基础。

关键词：雪茄烟遮阴栽培透光率叶面均匀性

Dynamics of crystallization and dissolution of calcium orthophosphates at the near-molecular level

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Chinese Science Bulletin 2011年第8期56卷 713-721页

作者： WANG LIJun LU JianWei xu fangsen ZHANG FuSuo College of Resources and Environment Huazhong Agricultural University Wuhan 430070 China College of Resources and Environmental Sciences China Agricultural University Beijing 100193 China

Although extensive investigations of biogenic and geological calcium phosphate crystallization/dissolution and their phase transformations have been performed,the mechanisms of crystallization and dissolution of sparingly soluble calcium phosphates in geological settings have not been completely determined at the near-molecular level.In particular,an understanding of the physical-chemical processes at the earliest nucleation stage and the subsequent crystal surface dynamics in soil solutions is lacking.This review focuses on the earliest events in homo/heterogeneous nucleation from an initial supersaturated solution phase,the subsequent growth of calcium phosphate phases,and the relevant influences of the presence of additional inorganic and organic molecules in both geological and biological settings.These studies have implications for the understanding of the complex processes of calcium phosphate transformations in soils,and provide possible physical-chemical mechanisms for the biogeochemical behavior of phosphorus at the near-molecular level.

关键词：钙磷酸盐分子水平溶解结晶物理化学过程生物环境地球化学行为土壤溶液

The dynamic surface evolution of halide perovskites induced by external energy stimulation

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National Science Review 2024年第4期11卷 185-194页

作者： Feiyu Cheng Pengdong Wang Chenzhe xu Qingliang Liao Suicai Zhang Haochun Sun Wenqiang Fan Guodong Liu Zhiyun Li Yaping Kong Li Wang fangsen Li Zhuo Kang Yue Zhang Academy for Advanced Interdisciplinary Science and Technology State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing Key Laboratory for Advanced Energy Materials and Technologies Key Laboratory of Advanced Materials and Devices for Post-Moore ChipsMinistry of EducationBeijing Advanced Innovation Center for Materials Genome Engineering School of Materials Science and EngineeringUniversity of Science and Technology Beijing Vacuum Interconnected Nanotech Workstation(Nano-X) Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of Sciences Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences

Tracking the dynamic surface evolution of metal halide perovskite is crucial for understanding the corresponding fundamental principles of photoelectric properties and intrinsic instability. However, due to the volatility elements and soft lattice nature of perovskites, several important dynamic behaviors remain unclear. Here, an ultra-high vacuum（UHV） interconnection system integrated by surface-sensitive probing techniques has been developed to investigate the freshly cleaved surface of CH3NH3PbBr3in situ under given energy stimulation. On this basis, the detailed three-step chemical decomposition pathway of perovskites has been clarified. Meanwhile, the evolution of crystal structure from cubic phase to tetragonal phase on the perovskite surface has been revealed under energy stimulation. Accompanied by chemical composition and crystal structure evolution, electronic structure changes including energy level position,hole effective mass, and Rashba splitting have also been accurately determined. These findings provide a clear perspective on the physical origin of optoelectronic properties and the decomposition mechanism of perovskites.

关键词： metal halide perovskite external energy stimulation surface dynamic evolution surface electronic structure ultra-high vacuum interconnection system

华中农业大学作物硼营养研究之历程与展望——纪念王运华先生逝世1周年

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华中农业大学学报 2023年第6期42卷 9-17页

作者：徐芳森华中农业大学资源与环境学院/微量元素研究中心武汉430070

硼是植物必需的微量营养元素,硼肥是国内施用最多的微量元素肥料。1975年王运华先生在湖北省新洲县发现农田大面积棉花“蕾而不花”症状为缺硼导致的结果,开启了华中农业大学作物硼营养的研究,至今已近50载。在近50 a的研究历程中,经历... 详细信息

硼是植物必需的微量营养元素,硼肥是国内施用最多的微量元素肥料。1975年王运华先生在湖北省新洲县发现农田大面积棉花“蕾而不花”症状为缺硼导致的结果,开启了华中农业大学作物硼营养的研究,至今已近50载。在近50 a的研究历程中,经历了既有明显特色又有相互交叉融合的4个阶段:(1)作物硼营养诊断与硼肥施用技术(1975-1985),该阶段主要建立了我国棉花硼营养诊断与硼肥施用技术;(2)作物硼的吸收利用与营养机制(1985-1995),该阶段以棉花和油菜为主要对象,研究揭示了作物对硼的吸收、转运、分配及其生理功能;(3)作物硼营养遗传与分子机制(1995-2015),该阶段研究以甘蓝型油菜为主要对象,筛选油菜硼高效品种,克隆硼高效基因,研究揭示植物响应缺硼胁迫的分子调控机制;(4)作物硼高效调控途径与利用(2015-),该阶段在深入研究油菜硼高效分子调控途径的同时,筛选培育硼高效育种材料和品种,开展高效品种与专用肥和先进栽培技术相结合的集成推广利用。不同阶段的研究成果为我国农业生产中微量元素肥料硼肥的推广应用和农作物增产增效做出了重要的贡献,并在国际上作物硼营养的应用基础和基础研究方面占有一席之地。在纪念王运华先生逝世1周年之际,针对这4个阶段的主要研究作回顾简述,缅怀先生对作物硼营养研究做出的巨大贡献,聊表笔者对先生的敬重之情。

关键词：作物硼营养诊断施肥技术吸收利用生理遗传

A further analysis of BnSPX3;2 promoter in Brassica napus indicated an unknown cis-acting element responsive to phosphorus stress

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A further analysis of BnSPX3;2 promoter in Brassica napus in...

第十四届全国植物基因组学大会

作者： Hongyuan Du Guangzhe Yang Guangda Ding Lei Shi fangsen xu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Phosphorus(P) is an essential macronutrient for plants.Because of the low availability of phosphate(Pi) absorbed directly by plant roots in soil,plants have evolved many adaptive responses.Recently the proteins carryi... 详细信息

关键词： Brassica napus SPX domain phosphate promoter cis-acting element

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植物硼营养高效的分子调控途径

华中农业大学学报 2023年第6期42卷 43-49页

作者：姜哲轩徐芳森华中农业大学资源与环境学院/微量元素研究中心武汉430070

植物体内的硼主要存在于细胞壁中,对稳定细胞壁结构和促进生长发育起重要作用。双子叶植物需硼多,对缺硼敏感,但不同物种及不同品种对缺硼的抗性存在极显著的基因型差异。华中农业大学王运华教授在1990年代带领团队开展甘蓝型油菜硼高... 详细信息

植物体内的硼主要存在于细胞壁中,对稳定细胞壁结构和促进生长发育起重要作用。双子叶植物需硼多,对缺硼敏感,但不同物种及不同品种对缺硼的抗性存在极显著的基因型差异。华中农业大学王运华教授在1990年代带领团队开展甘蓝型油菜硼高效品种的筛选,从此开启了我国植物硼营养高效利用的遗传与分子机制研究。近10多年的研究结果表明,植物响应缺硼胁迫提高硼效率存在2条不同的分子调控途径。(1)依赖硼转运基因的途径。在这条途径中,NIPs和BORs家族基因受缺硼诱导表达增强根系对土壤硼的吸收和体内硼的转运分配,实现硼的高效吸收和转运,进而提高植物对缺硼胁迫的抗性或适应性;(2)独立于硼转运基因的途径。该途径中,植物通过影响激素信号和细胞壁合成代谢相关基因的表达,调节根系生长发育和细胞壁组分结构等方式,提高体内硼的利用效率,进而增强植物对缺硼的抗性。在硼被确定为植物必需营养元素的百年纪念之际,我们对这一工作进行综述归纳,以飨读者。同时,在王运华先生逝世1周年之际,深切缅怀先生在开启华中农业大学作物硼营养遗传研究领域中所做的奠基性贡献。

关键词：硼甘蓝型油菜硼效率硼转运蛋白植物激素细胞壁