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全天候卫星微波观测资料变分同化研究进展

地球科学进展 2019年第11期34卷 1120-1130页

作者：窦芳丽陆其峰郭杨中国气象科学研究院北京100081 中国科学院大学北京100049 国家卫星气象中心中国气象局中国遥感卫星辐射测量和定标重点开放实验室北京100081

云和降水区微波观测包含大量与天气系统,特别是台风、暴雨等灾害性天气系统发生发展密切相关的大气信息,因此微波资料的全天候同化应用成为当前数值预报领域的热点研究问题。过去20年间,全球几大数值预报中心逐步开展了全天候同化技术... 详细信息

云和降水区微波观测包含大量与天气系统,特别是台风、暴雨等灾害性天气系统发生发展密切相关的大气信息,因此微波资料的全天候同化应用成为当前数值预报领域的热点研究问题。过去20年间,全球几大数值预报中心逐步开展了全天候同化技术的研究和业务应用,证实了全天候卫星微波观测资料能够改进模式中的质量、风场、湿度以及云和降水场的初始信息,从而改进数值预报模式的预报效果。通过梳理和评述全天候卫星微波观测资料同化方法,分析其中的关键技术问题和目前存在的困难和挑战,为未来在我国数值天气预报领域开展全天候同化研究提供依据。随着我国新一代数值天气预报模式的发展应用,加强我国全天候资料同化技术的研究将会在业务中发挥更大的科学效益和应用效益。

关键词：全天候云和降水微波观测资料同化一维变分

Enhanced therapeutic effect for murine colon carcinoma through Colon 26/Ag85A-CD226 tumor cell vaccine in BALB/c mice

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Enhanced therapeutic effect for murine colon carcinoma throu...

第九届全国免疫学学术大会

作者： Yan Li Jingbo Zhai fangli.Yang Lixuan Sang Junfeng Zhu Dan Yue Shengjun Li Xun Sun Changlong lu Department of Immunology China medical university

Objective:To examine the effects of Colon 26/Ag85A-CD226 tumor cell vaccine on colon carcinoma in ***:The recombinant pcDNA3.1-Ag85A-CD226 was constructed and stable transfected into the Colon 26 *** BALB/c mice were subcutaneously inoculated with Colon 26 cells and after three days,mice were immunized three times with Colon 26 cells,Colon 26/pcDNA3.1 cells,Colon 26/CD226 cells,Colon 26/Ag85A cells,or Colon 26/Ag85A-CD226 cells,which were inactivated by mitomycin C,at weekly intervals,*** immunotherapeutic effects by the tumor vaccines were evaluated by measuring the size of tumor and observing the survival of tumor-bearing mice,histopathological analysis and immunohistochemistry of CD4 or CD8 and TUNEL expression in tumor *** activities of NK cells and the tumor-specific cytotoxicity of CTL and The number of tumor-specific CD4and CD8T cells secreting IFN-γin spleen and MLNs were detected by ***:The survival rate of mice vaccinated with Colon 26/Ag85A-CD226 tumor cell vaccine was significantly increased,and the tumor volume and weight were the smallest in mice vaccinated with Colon 26/Ag85A-CD226 cell *** results of Massive necrosis in tumor tissues mice vaccinated with Colon 26/Ag85A-CD226 cell vaccine were *** expression and the infiltration of CD4or CD8T cell were increased in the Colon 26/Ag85A-CD226tumor cell vaccine treated mice as compared with that in other tumor cell vaccine treated *** activity of NK cell or CTL cytotoxicity in spleen of mice immunized with the Colon 26/Ag85A-CD226 tumor cell vaccine was significantly higher than other groups *** number of CD4IFN-γor CD8IFN-γT cells was significantly increased in spleen and MLN of mice immunized with the Colon 26/Ag85A-CD226 tumor cell vaccine as compared with that in other groups ***:The Colon 26/Ag85A-CD226 tumor cells vaccine treatment could protect mice from Colon Carcinoma through enhancing the antitumor immune respons

关键词： lon carcinoma Ag85A CD226 tumor cell vaccine

CD226 as a genetic adjuvant to enhance immune efficacy induced by Ag85A DNA vaccination

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CD226 as a genetic adjuvant to enhance immune efficacy induc...

第九届全国免疫学学术大会

作者： Di Zhang Yan Li fangli.Yang Junfeng Zhu Lixuan Sang Dan Yue Bing Wei Xun Sun Changlong lu Department of Immunology China Medical University

Objective:To construct the eukaryotic expressing plasmid of pcDNA3.1-Ag85A-CD226 and prepare Ag85A-CD226 DNA vaccine,and examine the effect of Ag85A DNA vaccine with CD226 as the genetic adjuvant on immune function in ***:The gene of CD226 was amplified from CD226-PCR2.1-ToPo by PCR and then inserted into constructed pcDNA3.1-Ag85A *** restriction enzyme analysis and sequence identification,the recombinant plasmid was transfected into HEK293 cells by *** extracting and purification recombinant,the Ag85A-CD226 DNA vaccine was prepared with liposomal *** mice were vaccinated vaccines by intragastric *** levels of mRNA and the protein of Ag85A or CD226 were identified by RT-PCR and *** or immunofluorescent assay was used to detect CD226 and Ag85A in small intestine,and the immune response induced by Ag85A-CD226 DNA vaccine was evaluated by detecting the activation of NK cell,NO level,cytokine production in spleen,or the cytokine mRNA level in intestine,*** number of CD4IFN-γor CD8IFN-γT cells in spleen and MLNs was detected by ***:Expression of CD226 and Ag85A in the epithelium of the small intestine was examined after the final ***226 protein was highly expressed in Peyer’s patches of the small *** activation of NK cells andthe level of NO in mice vaccinated with Ag85A-CD226 DNA vaccine were higher than those in other control *** protein or mRNA expression of TNF-α,IFN-γand IL-2 in spleen or intestine was higher,but the level of IL-4 was lower in spleen of mice vaccinated with Ag85A-CD226 DNA vaccine than those in the other group *** number of CD4IFN-γor CD8IFN-γT cells significantly increased in spleen and MLNs of mice immunized with pcDNA3.1-Ag85A-CD226 ***:The Ag85A-CD226 DNA vaccine could enhance Thl type response in systemic and intestinal immune function,as compared with Ag85A DNA vaccine or CD226 DNA ***

关键词： Ag85A CD226 genetic adjuvant DNA vaccine

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星载双频风场雷达热带气旋降雨区测风模拟

应用气象学报 2012年第4期23卷 467-477页

作者：窦芳丽卢乃锰谷松岩中国气象科学研究院北京100081 中国气象局中国遥感卫星辐射测量和定标重点开放实验室、国家卫星气象中心北京100081

基于大气辐射传输理论分别建立Ku波段和C波段的降雨模型,模拟热带气旋降雨区洋面的雷达回波并反演了洋面10 m风场,用于研究降雨对测风的影响以及风云三号双频风场雷达(WFR)的测风能力。分析表明:回波的衰减或增强取决于降雨衰减项和后... 详细信息

基于大气辐射传输理论分别建立Ku波段和C波段的降雨模型,模拟热带气旋降雨区洋面的雷达回波并反演了洋面10 m风场,用于研究降雨对测风的影响以及风云三号双频风场雷达(WFR)的测风能力。分析表明:回波的衰减或增强取决于降雨衰减项和后向散射项的相对大小;热带气旋的降雨使反演风速偏小,风向精度降低,Ku波段相对于C波段更易受影响,在高风速(超过30 m·s^(-1))条件下,可达5～20 m·s^(-1)的负风速偏差。反演结果表明:双频反演的新方法能够结合Ku波段与C波段的优势,双频最大似然估计(MLE)方法在分辨率上优于C波段单频反演,相对Ku单频反演能降低降雨对测风的衰减作用,结合双频MLE方法和C波段单频反演优势的分区反演方法可以显著减小降雨偏差,提高风速反演精度,在有风云三号湿度计同步观测的条件下,是提高热带气旋降雨区测风精度的有效手段。

关键词：降雨模型风场微波散射计辐射传输

IL-33 aggravates DSS-induced acute colitis by up-regulation of Th2 cytokine production in lamina propria of colon in mice.

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IL-33 aggravates DSS-induced acute colitis by up-regulation ...

第九届全国免疫学学术大会

作者： Dan Yue Junfeng Zhu fangli.Yang Lixuan Sang Yan Li Xiaoyun Sun Jingbo Zhai Xun Sun Changlong lu Department of Immunology China Medical University

Objectiv:Interleukin-33(IL-33)is a member of the IL-1 *** receptor,T1/ST2(ST2),a member of the IL-1 receptor family,is stably expressed on type 2 innate lymphoid cells(ILC2s)and T helper(Th)2 effector T *** levels of IL-33 are expressed by the intestinal epithelial cell suggesting that IL-33 may play a potential role in development of intestinal inflammation,In this study,we investigated the role of IL-33 in the pathogenesis of inflammatory bowel diseases(IBD).Methods:The recombinant mouse IL-33 was treated for C57BL/6 mice by intraperitoneal(i.p.)injection before and/or after dextran sodium sulfate(DSS)-induced *** induction of acute colitis mice received 3.5%DSS in their drinking water for 4 days and were intraperitoneally administrated IL-33(10μg/ml,100 ul/mouse/day)or PBS(100μl/mouse/day)at the same time,and then and diverse clinical signs of the colitis were evaluated,and lymphocyte population or the cytokine production in lamina propria of colon in IL-33-treated mice or control mice was detected by FACS or ***:IL-33 treatment resulted in aggravation of intestinal inflammation in mice with DSS-induced acute ***,the levels of IFN-γand IL-17A productions markedly reduced,but IL-5 and IL-13 secretions significantly increased in the lamina propria(LP)of colon in IL-33-treated mice as compared with those in control *** results suggest that IL-33 may stimulate CD4T cells to polarize towards a Th2 type response,but contrarily suppress Th17 and Th1 cells responses in large intestinal LP of ***:Our results suggest that IL-33 may play an importance role in the pathogenesis of DSS-induced acute colitis,which is a mouse colitis model similar to human ulcerative ***,modulation of IL-33 signaling by mAb could be a novel biological therapy for ulcerative colitis.

关键词： IL-33 Th2 Th1 Th17 acute colitis

Research on the change and interrelationship between intestine Th17 and Treg in colitis mouse model

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Research on the change and interrelationship between intesti...

第九届全国免疫学学术大会

作者： Xuefeng Jiang fangli.Yang Lixuan Sang Junfeng Zhu Yan Li Dan Yue Xiaoyun Sun Jingbo Zhai Xun Sun Changlong lu Department of Immunology China Medical University

Objective:To investigate the interrelationship between Th17 and Treg in the intestinal mucosa by detecting the numbers of Thl7 and Treg,and the expression of cytokine secretion in different genetic background mice after DSS-induced ***:Colitis was induced by administration of 3.5%dextran sodium sulfate(DSS)in drinking water to mice for *** were isolated from intestinal mucosa lamina propria(LP)and mesenteric lymph nodes(MLN).The numbers of Th17 and Treg were *** expression and production of Th17 and Treg relative cytokines were ***:C57BL/6 mice were susceptible to DSS-induced colitis as compared with Balb/*** mRNA expressions of IL-10 and FoxP3 were markedly lower,but the levels of IFN-γ,IL-6,IL-23 and IL-17a mRNA expressions were significantly higher in LP of C57BL/6 mice than those in Balb/c *** productions of IFN-γ,IL-6,IL-12,IL-23 and IL-17a were increased,but IL-10 was decreased in LP of C57BL/6 mice when compared with those in Balb/c *** numbers of CD4IFN-γcells and CD4IL-17Acells were increased,but the numbers of CD4CD25IL-10and CD4CD25FoxP3were decreased in LP of C57BL/6 mice as compared with those in Balb/c ***:These results suggest that the responses ofThl7and Thl were enhanced mice,but the response of Treg was reduced in the LP of inflammatory intestine and MLN in C57BL/6 mice as compared with Balb/c *** the disbalance of Th17/Treg may be involved in the development of DSS-induced colitis in different genetic background mice.

关键词： ulcerative colitis Th17 Treg lamina propria

合成生物学自动化装置iBioFoundry的构建与应用

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合成生物学 2023年第5期4卷 877-891页

作者：卢挥张芳丽黄磊浙江大学杭州国际科创中心杭州浙江311215 浙江大学生物工程研究所化学工程与生物工程学院浙江杭州310058

合成生物学是一门关于设计、改造和重新合成生命的交叉融合性学科。它以工程化理念对生物体进行有目标的设计、改造,使其拥有满足人类需求的生物功能,甚至创造新的“人造生命”。由于生命体的高度复杂性,为达预定目标往往需要进行大量... 详细信息

合成生物学是一门关于设计、改造和重新合成生命的交叉融合性学科。它以工程化理念对生物体进行有目标的设计、改造,使其拥有满足人类需求的生物功能,甚至创造新的“人造生命”。由于生命体的高度复杂性,为达预定目标往往需要进行大量人工试错性实验,导致研究成本高、进展缓慢。随着自动化合成生物技术的不断发展,目前全球各地有多个合成生物自动化设施已建成或在建中。本文从建设背景、设计构建、科研项目运行和应用前景等方面对浙江大学杭州国际科创中心建设的合成生物学自动化装置iBioFoundry进行介绍,通过大肠杆菌工程菌的批量构建和酶的定向进化及筛选案例对实验自动化方案制定、实验流程程序编写和上机运行的过程做简要描述和分析,并就装置耗材存储空间的分配、多实验任务并行和实验流程标准化建设等装置建设过程的一些思考做经验分享。合成生物学自动化装置可以帮助研究人员大幅提高实验效率,装置产生的大量高质量数据结合信息技术,有望高通量、低成本、多循环地实现合成生物学研究中“设计-构建-测试-学习”的自动化运行,加速合成生物学在基础及诸多应用领域的研究效率。

关键词：合成生物学生物铸造厂自动化平台标准化实验流程

Micro-CT对遗传性牙本质发育缺陷离体患牙的特征研究

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华西口腔医学杂志 2022年第2期40卷 162-168页

作者：韩安鹏鲁方丽陆玉平李强陈栋郑州大学第一附属医院河南省口腔医院口腔科郑州450052 南宁市柏乐口腔医院有限公司南宁530022 郑州颐和医院口腔科郑州450047 兰州大学口腔医院口腔科兰州730000

目的采用Micro-CT构建Ⅱ型牙本质发育不全(DGI-Ⅱ)和Ⅰ型牙本质发育不良(DD-Ⅰ)离体患牙的三维结构,研究该类患牙的内部结构及硬组织矿化密度的变化。方法收集同龄DGI-Ⅱ、DD-Ⅰ患者及健康者的第三磨牙,运用Micro-CT对3份样本逐一扫描... 详细信息

目的采用Micro-CT构建Ⅱ型牙本质发育不全(DGI-Ⅱ)和Ⅰ型牙本质发育不良(DD-Ⅰ)离体患牙的三维结构,研究该类患牙的内部结构及硬组织矿化密度的变化。方法收集同龄DGI-Ⅱ、DD-Ⅰ患者及健康者的第三磨牙,运用Micro-CT对3份样本逐一扫描并通过Mimics 17.0重建三维结构;截取矢状面和横断面观察患牙的内部结构;分别计算釉质、冠部牙本质以及根部牙本质的灰度值,分析牙齿不同部位的矿化密度。结果成功构建包括牙釉质帽、牙本质核和牙髓腔模型在内的DGI-Ⅱ、DD-Ⅰ患者的下颌第三磨牙三维精细模型;矢状面和横断面扫描显示DGI-Ⅱ患牙髓腔未完全钙化、根管狭窄,DD-Ⅰ患牙髓腔及根管闭锁、牙根缺如;灰度值分析发现DGI-Ⅱ、DD-Ⅰ患牙釉质、冠部牙本质及根部牙本质灰度值较正常对照组低(P0.05),根部牙本质的灰度值差异有统计学意义(P<0.01)。结论Micro-CT技术为遗传性牙本质发育缺陷离体患牙的三维结构重建及其矿化密度定量分析提供了简单而精确的方法。其中DGI-Ⅱ、DD-Ⅰ患牙的牙本质矿化密度均降低,DD-Ⅰ患牙降低更显著,髓腔内均有异常钙化物质,根管狭窄甚至闭塞。

关键词： Micro-CT 牙本质发育不全牙本质发育不良三维重建牙齿矿化

IL-33 alleviate DSS-induced chronic colitis by suppressing Th1/Th17 cytokine production in lamina propria of colon in mice

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IL-33 alleviate DSS-induced chronic colitis by suppressing T...

第九届全国免疫学学术大会

作者： Junfeng Zhu fangli.Yang Lixuan Sang Dan Yue Yan Li Jingbo Zhai Xiaoyun Sun Shengjun Li Xun Sun Changlong lu Department of Immunology China Medical University

Objective Interleukin(IL)-33,belonging to the IL-1 cytokine family member,is an important modulator of the immune system associated with several autoimmune ***-33 expresses high level in endothelial cells and epithelial cells of intestinal ***,IL-33 may play an important role in the development of intestinal *** this study,we investigated the effect of IL-33 on DSS-induced chronic colitis in ***:For chronic colitis induction,mice were administered with 1.5%DSS in the drinking water on days 0-5,10-15 and 20-25,and were treated by intraperitoneally administrated IL-33(10 Hg/ml,100 ul/mouse/day)or PBS(100 ul/mouse/day)for 5 days after the end of each DSS cycle,and then diverse clinical signs of the colitis were evaluated,and lymphocyte population or the cytokine production in lamina propria of colon in IL-33-treated mice or control mice was detected by FACS or ***:IL-33 treatment resulted in alleviation of intestinal inflammation in mice with DSS-induced chronic ***,the levels of IFN-y and IL-17A productions markedly reduced,but IL-5 and IL-13 secretions increased in the lamina propria(LP)of colon in IL-33-treated mice as compared with those in control *** results suggest that IL-33 may play a protective role in DSS-induced chronic colitis by switching a predominantly pathogenic Thl/Thl7 response to Th2 ***:Our results suggest that IL-33 may play an importance role in the recovery of DSS-induced chronic colitis,which is a mouse colitis model similar to human chronic Crohn’s disease(CD).Furthermore,IL-33 could be a novel biological therapy target for CD.

关键词： IL-33 Th2 Th1 Th17 chronic colitis