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Chinese Chemical Letters 2024年第6期35卷 439-444页

作者： Zhichao Zhou Fuqian Chen Xiaotong Xia Dong Ye Rong Zhou Lei Li Tao deng Zhenhua Ding Fang Liu department of Radiation Medicine Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou 510515China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China Artemisinin Research Center Guangzhou University of Chinese MedicineGuangzhou 510006China State key laboratory of respiratory disease the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhou 510120China guangzhou laboratory Bio-IslandGuangzhou 510030China Nanshan Pharmaceutical Innovation Research institute of guangdong Province Foshan 528225China

The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or Amplex red)is the most sensitive fluorogenic substrate known for HRP in current market,however,it suffers from some drawbacks,such as non-specific reactivity to carboxylesterase and limited fluorescence *** the present study,a novel HRP substrate10-cyclopropylcarbonyl-dichloro-dihydroxyphenoxazine(AR-2),has been prepared,which exhibited improved sensitivity than ADHP in sensing ***,the fluorescence of AR-2/HRP demonstrated improved tolerance to physiological relevant p H fluctuation as compared to ADHP/*** detection of uric acid/urate oxidase reaction indicated excellent application prospect of AR-2/HRP for monitoring H_(2)O_(2)-generating biochemical *** interestingly,an enzyme-linked immunosorbent assay(ELISA)using AR-2 as the fluorescence reporter has been successfully used in detecting IgG against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human serum ***,AR-2 exhibits improved performances over the commercial ADHP,which will be an ideal alternative to ADHP in HRP-based fluorescence biochemical analysis and immunoassays.

关键词： Fluorescence immunoassay HRP fluorescence substrate Hydroresorufin Amplex red/ADHP SARS-CoV-2

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中国咳嗽基层诊疗与管理指南(2024年)

中华全科医师杂志 2024年第8期23卷 793-812页

作者：中华医学会中华医学会杂志社中华医学会全科医学分会中华医学会呼吸病学分会哮喘学组中华医学会《中华全科医师杂志》编辑委员会中国呼吸系统疾病基层诊疗与管理指南制定专家组赖克方迟春花不详呼吸疾病全国重点实验室、国家呼吸系统疾病临床医学研究中心、国家呼吸医学中心、广州呼吸健康研究院、广州医科大学附属第一医院呼吸与危重症医学科广州医科大学广州510000 北京大学第一医院全科医学科北京大学第一医院健康管理中心北京100034

咳嗽是门诊患者最常见的症状。在国内专科门诊中,慢性咳嗽患者占1/3以上[1]。咳嗽病因复杂且涉及面广,明确诊断需结合多项检查。很多患者常因诊断不明确,反复进行各种检查或者长期使用抗菌药物和镇咳药物,收效甚微并产生诸多不良反应,... 详细信息

咳嗽是门诊患者最常见的症状。在国内专科门诊中,慢性咳嗽患者占1/3以上[1]。咳嗽病因复杂且涉及面广,明确诊断需结合多项检查。很多患者常因诊断不明确,反复进行各种检查或者长期使用抗菌药物和镇咳药物,收效甚微并产生诸多不良反应,对患者的工作、学习和生活质量造成严重影响,同时也带来了严重的卫生经济负担[2,3,4]。

关键词：咳嗽诊断治疗疾病管理指南基层

乙酰甲胆碱(氯醋甲胆碱)支气管激发试验技术规范(2023年版)

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中华结核和呼吸杂志 2024年第2期47卷 101-119页

作者：中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会中华医学会呼吸病学分会中国老年医学会呼吸分会肺功能学组郑劲平宋元林高怡黄刚李丽梁斌苗逯勇谢燕清赵桂华不详国家呼吸医学中心、国家呼吸疾病临床医学研究中心、呼吸疾病全国重点实验室、广州呼吸健康研究院、广州医科大学附属第一医院呼吸与危重症医学科广州510120 复旦大学附属中山医院呼吸与危重症医学科上海200032 广州医科大学附属第一医院浙江大学医学院附属第二医院复旦大学附属中山医院四川大学华西医院首都医科大学附属北京朝阳医院河南省人民医院

乙酰甲胆碱支气管激发试验(MCT)是评估气道高反应性及其程度的常用检查,对支气管哮喘诊断及治疗效果评估具有重要临床价值。中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会、中华医学会呼吸病学分会肺功能学组(筹)和中国老年... 详细信息

乙酰甲胆碱支气管激发试验(MCT)是评估气道高反应性及其程度的常用检查,对支气管哮喘诊断及治疗效果评估具有重要临床价值。中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会、中华医学会呼吸病学分会肺功能学组(筹)和中国老年医学会呼吸分会肺功能学组的专家们共同组成共识工作组,基于2014年发布的《肺功能指南——支气管激发试验检查》及其在使用中存在的问题与近年研究进展,在充分收集专家组意见、查阅文献、问卷调研、多轮线上和线下讨论后,形成了《乙酰甲胆碱(氯醋甲胆碱)支气管激发试验技术规范(2023年版)》。本共识收集了国内MCT临床实践中核心的11个问题并形成推荐意见,内容包括MCT的适应证与禁忌证、激发试剂配制、试验流程与方法、质量控制、安全性管理、结果解读和报告规范等方面,为医疗机构临床肺功能从业人员开展MCT提供参考,以期更好应用该项技术指导临床诊疗。

关键词：支气管激发试验安全性管理中国医师协会中华医学会推荐意见乙酰甲胆碱问卷调研气道高反应性

A Cullin 5-based complex serves as an essential modulator of ORF9b stability in SARS-CoV-2 replication

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3112-3129页

作者： Yuzheng Zhou Zongpeng Chen Sijie Liu Sixu Liu Yujie Liao Ashuai Du Zijun Dong Yongxing Zhang Xuan Chen Siyi Tao Xin Wu Aroona Razzaq Gang Xu De-an Tan Shanni Li Youwen deng Jian Peng Shuyan Dai Xu deng Xianwen Zhang Taijiao Jiang Zheng Zhang Gong Cheng Jincun Zhao Zanxian Xia department of Cell Biology School of Life SciencesCentral South University410013 ChangshaChina institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated HospitalSchool of MedicineSouthern University of Science and Technology518112 ShenzhenChina department of Basic Medicine School of MedicineHunan Normal University410081 ChangshaChina department of spine surgery The Third Xiangya HospitalCentral South University410013 ChangshaChina School of Basic medical Sciences Anhui Medical University230032 HefeiChina Hunan key laboratory of Neurorestoratology 921 Hospital of Joint Logistics Support Force People’s Liberation Army of China(The Second Affiliated Hospital of Hunan Normal University)410003 ChangshaHunanChina department of Geriatric Surgery Xiangya HospitalCentral South University410008 ChangshaChina Xiangya School of Pharmaceutical Sciences Central South University410013 ChangshaChina institute of Infectious diseases Shenzhen Bay Laboratory518132 ShenzhenChina guangzhou laboratory 510005 GuangzhouChina Tsinghua University-Peking University Joint Center for Life Sciences School of MedicineTsinghua University100084 BeijingChina State key laboratory of respiratory disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical University510120 GuangzhouChina Hunan key laboratory of Animal Models for Human diseases Hunan Key Laboratory of Medical Genetics&Center for Medical GeneticsSchool of Life SciencesCentral South University410008 ChangshaChina

The ORF9b protein,derived from the nucleocapsid’s open-reading frame in both SARS-CoV and SARS-CoV-2,serves as an accessory protein crucial for viral immune evasion by inhibiting the innate immune *** its significance,the precise regulatory mechanisms underlying its function remain *** the present study,we unveil that the ORF9b protein of SARS-CoV-2,including emerging mutant strains like Delta and Omicron,can undergo ubiquitination at the K67 site and subsequent degradation via the proteasome pathway,despite certain mutations present among these ***,our investigation further uncovers the pivotal role of the translocase of the outer mitochondrial membrane 70(TOM70)as a substrate receptor,bridging ORF9b with heat shock protein 90 alpha(HSP90α)and Cullin 5(CUL5)to form a *** this complex,CUL5 triggers the ubiquitination and degradation of ORF9b,acting as a host antiviral factor,while HSP90αfunctions to stabilize ***,treatment with HSP90 inhibitors such as GA or 17-AAG accelerates the degradation of ORF9b,leading to a pronounced inhibition of SARS-CoV-2 ***-cell sequencing data revealed an up-regulation of HSP90αin lung epithelial cells from COVID-19 patients,suggesting a potential mechanism by which SARS-CoV-2 may exploit HSP90αto evade the host *** study identifies the CUL5-TOM70-HSP90αcomplex as a critical regulator of ORF9b protein stability,shedding light on the intricate host–virus immune response dynamics and offering promising avenues for drug development against SARS-CoV-2 in clinical settings.

关键词： stability modulator offering

Initial COVID-19 severity influenced by SARS-CoV-2-specific T cells imprints T-cell memory and inversely affects reinfection

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2791-2803页

作者： Gang Yang Jinpeng Cao Jian Qin Xinyue Mei Shidong deng Yingjiao Xia Jun Zhao Junxiang Wang Tao Luan Daxiang Chen Peiyu Huang Cheng Chen Xi Sun Qi Luo Jie Su Yunhui Zhang Nanshan Zhong Zhongfang Wang The affiliated hospital of Kunming University of Science and Technology.department of respiratory and Critical Care Medicine The First People’s Hospital of Yunnan provinceKunmingYunnanChina guangzhou national laboratory BiolandGuangzhouGuangdongChina State key laboratory of respiratory disease&national Clinical Research Center for respiratory disease Guangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouChina

The immunoprotective components control COVID-19 disease severity,as well as long-term adaptive immunity maintenance and subsequent reinfection risk discrepancies across initial COVID-19 severity,remain ***,we longitudinally analyzed SARS-CoV-2-specific immune effectors during the acute infection and convalescent phases of 165 patients with COVID-19 categorized by *** found that early and robust SARS-CoV-2-specific CD4^(+)and CD8^(+)T cell responses ameliorate disease progression and shortened hospital stay,while delayed and attenuated virus-specific CD8^(+)T cell responses are prominent severe COVID-19 *** antiviral antibody generation rather than titer level associates with severe ***,initial COVID-19 severity imprints the long-term maintenance of SARS-CoV-2-specific adaptive immunity,demonstrating that severe convalescents exhibited more sustained virus-specific antibodies and memory T cell responses compared to mild/moderate ***,initial COVID-19 severity inversely correlates with SARS-CoV-2 reinfection ***,our study unravels the complicated interaction between temporal characteristics of virus-specific T cell responses and COVID-19 severity to guide future SARS-CoV-2 wave management.

关键词： severity immunity infection

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大鼠血流缓慢模型的制备

血栓与止血学 2024年第4期30卷 169-174页

作者：张勇彬陈伟毅阮薇林伟国苏佳琪邓方阁广州医科大学附属第一医院/广州呼吸健康研究院/呼吸疾病全国重点实验室/国家呼吸系统疾病临床医学研究中心红外项目组广州510120 梅州市人民医院超声科梅州514031 广州市干部和人才健康管理中心医学影像中心广州510700

目的通过皮下反复注射肾上腺素、冰水游泳后观察血液流变学指标和双下肢超声结果,以构建血栓形成前的大鼠血流缓慢模型。方法选取12只健康SD大鼠,肾上腺素组(n=6)皮下注射0.8 mL/kg肾上腺素,2 h后冰水游泳5 min,注射4 h后再次注射,并禁... 详细信息

目的通过皮下反复注射肾上腺素、冰水游泳后观察血液流变学指标和双下肢超声结果,以构建血栓形成前的大鼠血流缓慢模型。方法选取12只健康SD大鼠,肾上腺素组(n=6)皮下注射0.8 mL/kg肾上腺素,2 h后冰水游泳5 min,注射4 h后再次注射,并禁食16 h。生理盐水组(n=6)皮下注射0.8 mL/kg生理盐水,其制备方法与肾上腺素组一致。造模完成后,行双下肢股静脉超声和血液流变学检查。结果两组大鼠双下肢均无明显肿胀。在冰水游泳第1 min内,肾上腺素组大鼠较生理盐水组兴奋(P=0.015)。超声结果显示,两组大鼠股静脉均无明显血栓形成,但是肾上腺素组的右侧股静脉流速比生理盐水组低(P1)。血液流变学结果显示,肾上腺素组大鼠的全血粘度低切(P=0.024)及血浆粘度(P=0.004)较生理盐水组高。结论通过大鼠皮下反复注射肾上腺素及冰水游泳,并利用血液流变学和超声检查,可以构建大鼠血流缓慢模型。

关键词：血流缓慢肾上腺素冰水游泳深静脉血栓形成

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咳嗽公众教育中国专家共识(第一版)

中华全科医师杂志 2024年第4期23卷 331-344页

作者：中华医学会全科医学分会中华医学会杂志社中华医学会《中华全科医师杂志》编辑委员会《咳嗽公众教育中国专家共识》编写专家组迟春花赖克方陈亚红代继宏范腾阳孔慜李光熙邱忠民姚弥易芳张建欣周建英吴浩杨汀不详北京大学第一医院全科医学科北京大学第一医院健康管理中心北京100034 广州医科大学附属第一医院呼吸与危重症医学科、国家呼吸医学中心、国家呼吸系统疾病临床医学研究中心、呼吸疾病全国重点实验室、广州呼吸健康研究院广州510120 北京大学第三医院重庆医科大学附属儿童医院遵义医科大学附属医院北京市丰台区方庄社区卫生服务中心中国中医科学院广安门医院同济大学附属同济医院北京大学第一医院广州医科大学附属第一医院浙江大学医学院附属第一医院首都医科大学全科医学与继续教育学院中日友好医院

咳嗽是人体一种重要的保护性反射行为。通过咳嗽可将呼吸道内的痰液排出体外,保持呼吸道的清洁状态,以及防止异物(如花生米等)进入气道[1],因此正常适度的咳嗽对人体有益且很有必要。

关键词：咳嗽健康教育公众共识

An overview for monitoring and prediction of pathogenic microorganisms in the atmosphere

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Fundamental Research 2024年第3期4卷 430-441页

作者： Jianping Huang Danfeng Wang Yongguan Zhu Zifeng Yang Maosheng Yao Xiaoming Shi Taicheng An Qiang Zhang Cunrui Huang Xinhui Bi Jiang Li Zifa Wang Yongqin Liu Guibing Zhu Siyu Chen Jian Hang Xinghua Qiu Weiwei deng Huaiyu Tian Tengfei Zhang Tianmu Chen Sijin Liu Xinbo Lian Bin Chen Beidou Zhang Yingjie Zhao Rui Wang Han Li Collaborative Innovation Center for Western Ecological Safety College of Atmospheric SciencesLanzhou UniversityLanzhou 730000China Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China national Clinical Research Center for respiratory disease Guangzhou Institute of Respiratory HealthFirst Affiliated Hospital of Guangzhou Medical UniversityState Key Laboratory of Respiratory Disease(Guangzhou Medical University)Guangzhou 510230China college of Environmental Sciences and Engineering Peking UniversityBeijing 100871China China CDC key laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China guangdong-Hong Kong-Macao Joint laboratory for Contaminants Exposure and Health School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China Ministry of Education key laboratory for Earth System Modeling Department of Earth System ScienceTsinghua UniversityBeijing 100084China Vanke School of Public Health Tsinghua UniversityBeijing 100084China State key laboratory of Organic Geochemistry and guangdong key laboratory of Environmental Protection and Resources Utilization Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China national Cancer Center/national Clinical Research Center for Cancer/Cancer hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China State key laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Center for Pan-third Pole Environment Lanzhou UniversityLanzhou 730000China college of Atmospheric Sciences Lanzhou UniversityLanzhou 730000China School of Atmospheric Sciences Sun Yat-sen Universityand Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 510640

Corona virus disease 2019(COVID-19)has exerted a profound adverse impact on human *** have demonstrated that aerosol transmission is one of the major transmission routes of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Pathogenic microorganisms such as SARS-CoV-2 can survive in the air and cause widespread infection among *** monitoring of pathogenic microorganism transmission in the atmosphere and accurate epidemic prediction are the frontier guarantee for preventing large-scale epidemic *** of pathogenic microorganisms in the air,especially in densely populated areas,may raise the possibility to detect viruses before people are widely infected and contain the epidemic at an earlier *** multi-scale coupled accurate epidemic prediction system can provide support for governments to analyze the epidemic situation,allocate health resources,and formulate epidemic response *** review first elaborates on the effects of the atmospheric environment on pathogenic microorganism transmission,which lays a theoretical foundation for the monitoring and prediction of epidemic ***,the monitoring technique development and the necessity of monitoring pathogenic microorganisms in the atmosphere are summarized and ***,this review introduces the major epidemic prediction methods and highlights the significance to realize a multi-scale coupled epidemic prediction system by strengthening the multidisciplinary cooperation of epidemiology,atmospheric sciences,environmental sciences,sociology,demography,*** summarizing the achievements and challenges in monitoring and prediction of pathogenic microorganism transmission in the atmosphere,this review proposes suggestions for epidemic response,namely,the establishment of an integrated monitoring and prediction platform for pathogenic microorganism transmission in the atmosphere.

关键词： Aerosol transmission Epidemic monitoring methods Epidemic prediction modelsEpidemic response Multidisciplinary cooperation

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红外热成像在乳腺疾病检测的应用研究

红外技术 2020年第5期42卷 501-505页

作者：曾靓妮邓方阁广州医科大学附属第一医院广州呼吸健康研究院呼吸疾病国家重点实验室国家呼吸系统疾病临床医学研究中心广东广州510120

乳腺疾病已严重危害女性身心健康,其中乳腺癌更位居全球范围内女性癌症发病率和死亡率首位,因此乳腺癌的早期发现意义重大。传统结构影像学早期检测疾病具有一定局限性,而红外热成像作为功能成像技术可为乳腺癌的早期筛查提供有效线索... 详细信息

乳腺疾病已严重危害女性身心健康,其中乳腺癌更位居全球范围内女性癌症发病率和死亡率首位,因此乳腺癌的早期发现意义重大。传统结构影像学早期检测疾病具有一定局限性,而红外热成像作为功能成像技术可为乳腺癌的早期筛查提供有效线索。因此本文主要就红外热成像在乳腺疾病的早期检测及预后评估的应用价值进行综述。

关键词：红外热成像乳腺疾病早期发现预后评估