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Efficacy profiles for different concentrations of Lactobacillus acidophilus in experimental colitis

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World Journal of Gastroenterology 2013年第32期19卷 5347-5356页

作者： Lin-Lin Chen Yi-You Zou fang-gen lu Fu-Jun Li Guang-Hui Lian Department of Gastroenterology Xiangya Hospital of Central South University Department of Gastroenterology Second Xiangya Hospital of Central South University

AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(***)for treating colitis using an experimental murine ***:Colitis was established in 64 BALB/c mice by adding 5%dextran sodium sulfate(DSS)to the drinking water and allowing ad libitum access for 7 *** mice were then randomly divided into the following control and experimental model groups(n=8 each;day 0):untreated model control;negative-treatment model control(administered gavage of 1 mL/10 g normal saline);experimental-treatment models C4-C8(administered gavage of 104,105,106,107,or 108CFU/10 g ***,respectively);positive-treatment model control(administration of the anti-inflammatory agent prednisone acetate at 45 g/10 g).Eight mice given regular water(no DSS)and no subsequent treatments served as the normal control *** weight,fecal traits,and presence of fecal occult blood were assessed *** animals were sacrificed on post-treatment day7 to measure colonic length,perform histological scoring,and quantify the major bacteria in the proximal and distal *** differences were determined by one-way ANOVA and post-hoc Student-Newman-Keuls ***:All treatments(*** and prednisone acetate)protected against colitis-induced weight loss(PC4>C5>C7>C8>C6;P0.05).The C6 group also had the lowest disease activity index scores among the model *** bacterial profiles in the proximal colon were similar between all of the experimental-treatment model groups(all P>0.05).In contrast,the bacterial profile in the distal colon of the C6 group showed the distinctive features(Plus

关键词： Lactobacillus acidophilus Bifidobacterium Colonic flora Therapeutic dose Experimental colitis Efficacy profile

Ulcerative colitis as a polymicrobial infection characterized by sustained broken mucus barrier

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World Journal of Gastroenterology 2014年第28期20卷 9468-9475页

作者： Shui-Jiao Chen Xiao-Wei Liu Jian-Ping Liu Xi-Yan Yang fang-gen lu Department of Gastroenterology 2nd Xiangya Hospital of Central South University

To reduce medication for patients with ulcerative colitis(UC),we need to establish the etiology of *** intestinal microbiota of patients with inflammatory bowel disease(IBD)has been shown to differ from that of healthy controls and abundant data indicate that it changes in both composition and *** intestinal bacterial overgrowth is significantly higher in IBD patients compared with *** have been investigated for their capacity to reduce the severity of *** luminal surfaces of the gastrointestinal tract are covered by a mucus *** normally acts as a barrier that does not allow bacteria to reach the epithelial cells and thus limits the direct contact between the host and the *** mucus layer in the colon comprises an inner layer that is firmly adherent to the intestinal mucosa,and an outer layer that can be washed off with minimal *** bacteria can dissolve the protective inner mucus *** in renewal and formation of the inner mucus layer allow bacteria to reach the epithelium and have implications for the causes of *** this review,important elements of UC pathology are thought to be the intestinal bacteria,gut mucus,and the mucosa-associated immune system.

关键词： Ulcerative colitis Mucus Infection Bacteria Etiology

Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses

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World Journal of Gastroenterology 2012年第19期18卷 2344-2356页

作者： Fu-Xi Zhou lu Chen Xiao-Wei Liu Chun-Hui Ouyang Xiao-Ping Wu Xue-Hong Wang Chun-Lian Wang fang-gen lu Division of Digestive Disease Xiangya Second-HospitalCentral South UniversityChangsha 410011Hunan ProvinceChina

AIM： TO investigate the role of Lactobacillus crispatus （L. crispatus） strain China Center for Type Culture Col- lection （CCTCC） M206119 in intestinal inflammation.METHODS： Forty 8-wk-old Balb/c mice （20± 2 g） were divided into four groups of 10 mice each. Three groups that had received dextran sulfate sodium （DSS） were administered normal saline, sulfasalazine or CCTCC M206119 strain, and the fourth group received none of these. We assessed the severity of colitis using a disease activity index, measured the colon length and weight, collected stools and mesenteric lymph nodes for bacterial microflora analysis. One centimeter of the proximal colon, middle colon and distal colon were collected and fixed in 10% buffered formalin, dehydrated in ethanol, and embedded in paraffin. Interleukin （IL）- 1β, IL-6 and tumor necrosis factor （TNF）-α expression was detected using reverse transcription polymerase chain reaction. Protective factors zonula occludens （ZO）-1 and β-defensin 2 were detected by immunoblot-ting. The features of CCTCC M206119 strain were identified based on morphology, biochemical profile, and 16S RNA sequencing.RESULTS： DSS-colitis animals treated with CCTCC M206119 had markedly more severe disease, with greater weight loss, diarrhea, fecal bleeding, and shortened colon length. In addition, the CCTCC-M206119- treated group had comparatively higher histologi- cal scores and more neutrophil infiltration than the controls. Expression of protective factors ZO-1 and β-defensin 2 was downregulated due to destruction of the mucosal barrier after CCTCC M206119 strain treatment. An in vitro assay demonstrated that CCTCC M206119 strain increased the nuclear translocation of nuclear factor-κB in epithelial cells. Intestinal proinflam- matory or anti-inflammatory cytokine responses were evaluated. Proinflammatory colonic cytokine （IL-Iβ, IL-6 and TNF-α） levels were clearly increased in CCTCC- M206119-treated animals, whereas anti-inflammatory colonic cytokine （IL-

关键词： Colitis Lactobacillus crispatus Intestine Dextra sodium sulfate Mice

Therapeutic effects of four strains of probiotics on experimental colitis in mice

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World Journal of Gastroenterology 2009年第3期15卷 321-327页

作者： Lin-Lin Chen Xue-Hong Wang Yi Cui Guang-Hui Lian Jie Zhang Chun-Hui Ouyang fang-gen lu Department of Gastroenterology The Second Xiangya Hospital of Central South University Changsha 410011 Hunan Province China Department of Gastroenterology Zhongshan City People's Hospital Zhongshan 528400 Guangdong Province China

AIM:To investigate the therapeutic effects of four strains of probiotics(***,***,*** and ***) on dextran sulphate sodium(DSS)-induced experimental colitis in Balb/c ***:Eighty Balb/c mice were randomly divided into 8 ***-loss,fecal character,fecal occult blood and hematochezia were recorded *** activity index(DAI) scores were also evaluated *** of colon was measured and histological scores were evaluated on the 13th ***(MPO) activity was ***-1(IL-1) and IL-4 expression was detected by ELISA and ***:The four strains of probiotics relieved the inflammatory condition of DSS-induced experimental colitis in *** loss was slowed down in all probiotics-treated *** weight gain was observed by the end of probiotics *** DAI and histological scores of probiotics-treated mice were lower than those of mice in the control group(1.9 ± 0.2 vs 8.6 ± 0.4,P < 0.05 for ***).The length of colon of probiotics-treated mice was longer than that of mice in the control group(10.3 ± 0.34 vs 8.65 ± 0.77,P < 0.05 for ***).The four strains of probiotics decreased the MP activity and the IL-1 expression,but increased the IL-4 *** had a better effect on DSS-induced experimental colitis in mice than the other three ***:The four strains of probiotics have beneficial effects on experimental colitis in *** has a better effect on DSS-induced experimental colitis in mice than the other three *** of probiotics provides a new therapy for UC.

关键词： Probiotic E faecalis Experimental colitis Interleukin-1 Interleukin-4

Primary shunt hyperbilirubinaemia in a large four-generation family confirming autosomai dominant genetic disorder

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World Journal of Gastroenterology 2006年第19期12卷 3123-3125页

作者： Chun-Lian Wang Xiao-Wei Liu fang-gen lu Xiao-Ping Wu Chun-Hui Ouyang Dong-Ye Yang Department of Gastroenterology the Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China

AIM： To describe the pattern of inheritance and confirm the diagnostic criteda of primary shunt hyperbilirubinaemia （PSH）. METHODS： Forty members of a family pedigree across four generations were included in this study. All family members were interviewed and investigated by physical examination, hematology and liver function test and the pattern of inheritance was analyzed. RESULTS： Nine of the forty family members suffered primary shunt hyperbilirubinaemia. The mature erythrocytes of the propositus were irregular in shape and size. The pedigree showed transmission of the trait through four generations with equal distribution in male and female. No individual with a primary shunt hyperbilirubinaemia was born to unaffected parents. The penetrance was complete in adult. CONCluSION： The pattern of inheritance is autosomal dominant. The abnormality of erythrocytes and decrease in white blood cell could be supplemented in the diagnosis of PSH. The PSH is a genetic disorder and could by renamed as hereditary shunt hyperbilirubinaemia.

关键词： Hyperbilirubinaemia Hereditary Shunt

Lactobacillus species shift in distal esophagus of high-fat-diet-fed rats

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World Journal of Gastroenterology 2011年第26期17卷 3151-3157页

作者： Xin Zhao Xiao-Wei Liu Ning Xie Xue-Hong Wang Yi Cui Jun-Wen Yang Lin-Lin Chen fang-gen lu Department of Gastroenterol-ogy The Second Xiangya Hospital of Central South UniversityChangsha 410011 Hunan Province China Department of Gastroenterology Xiangya Hospi-tal of Central South University Changsha 410008 Hunan Prov-ince China

AIM： To analyze the microbiota shift in the dista esophagus of Sprague-Dawley rats fed a high-fat diet. METHODS： Twenty Sprague-Dawley rats were divided into high-fat diet and normal control groups of 10 rats each. The composition of microbiota in the mucosa from the distal esophagus was analyzed based on se- lective culture. A variety of Lactobacillus species were identified by molecular biological techniques. Bacterial DNA from Lactobacillus colonies was extracted, and 165 rDNA was amplified by PCR using bacterial uni- versal primers. The amplified 16S rDNA products were separated by denaturing gradient gel electrophoresis （DGGE）. Every single band was purified from the gel and sent to be sequenced. RESULTS： Based on mucosal bacterial culturing in the distal esophagus, Staphylococcus aureus was absent, and total anaerobes and Lactobacillus species were de- creased significantly in the high-fat diet group compared with the normal control group （P 〈 0.01）. Detailed DGGE analysis on the composition of Lactobacillus species in the distal esophagus revealed that Lactobacillus crispa- tus, Lactobacillus gasseri （L. gasser/] and Lactobacillus reuteri （L. reuterl] comprised the Lactobacillus species in the high-fat diet group, while the composition of Lactobacillus species in the normal control group consisted of L. gasseri, Lactobacillus jensenii and L. reuteri. CONCluSION： High-fat diet led to a mucosal micro- flora shift in the distal esophagus in rats, especially the composition of Lactobacillus species.

关键词： Obesity Lactobacillus Sprague-Dawleyrats Distal esophagus Denaturing gradient gel electro-phoresis

Effects of different diets on intestinal microbiota and nonalcoholic fatty liver disease development

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World Journal of Gastroenterology 2016年第32期22卷 7353-7364页

作者： Jian-Ping Liu Wen-Li Zou Shui-Jiao Chen Hong-Yun Wei Ya-Ni Yin Yi-You Zou fang-gen lu Department of Gastroenterology Second Xiangya Hospital of Central South University Department of Nephrology Zhejiang Provincial People’s Hospital Department of Gastroenterology Xiangya Hospital of Central South University

AIM To study the effects of different diets on intestinal microbiota and nonalcoholic fatty liver disease(NAFLD) development at the same caloric *** Thirty male Sprague-Dawley rats were randomized into five groups(six rats each). The control diet(CON) group and free high-fat diet(FFAT) group were allowed ad libitum access to a normal chow diet and a highfat diet, respectively. The restrictive high-fat diet(RFAT) group, restrictive high-sugar diet(RSUG) group, and high-protein diet(PRO) group were fed a highfat diet, a high-sugar diet, and a high-protein diet, respectively, in an isocaloric way. All rats were killed at 12 wk. Body weight, visceral fat index(visceral fat/body weight), liver index(liver/body weight), insulin resistance, portal lipopolysaccharide(LPS), serum alanine aminotransferase(ALT), serum aspartate aminotransferase(AST), and liver triglycerides were measured. The intestinal microbiota in the different groups of rats was sequenced using high-throughput sequencing *** The FFAT group had higher body weight, visceral fat index, liver index, peripheral insulin resistance, portal LPS, serum ALT, serum AST, and liver triglycerides compared with all other groups(P < 0.05). Taking the same calories, the RFAT and RSUG groups demonstrated increased body weight, visceral fat index, peripheral insulin resistance and liver triglycerides compared with the PRO group(P < 0.05). The RFAT group also showed increased portal LPS compared with the PRO group(P < 0.05). Unweighted Uni Frac principal coordinates analysis of the sequencing data revealed that the intestinal microbiota structures of the CON, FFAT, RSUG and PRO groups were roughly separated away from each other. Taxon-based analysis showed that, compared with the CON group, the FFAT group had an increased abundance of Firmicutes, Roseburia and Oscillospira bacteria, a higher ratio of Firmicutes to Bacteroidetes, and a decreased abundance of Bacteroidetes, Bacteroides and Parabacteroi

关键词： Nonalcoholic fatty liver disease High-fat diet Restrictive high-fat diet Restrictive high-sugar diet High-protein diet Intestinal microbiota

GPC3 fused to an alpha epitope of HBsAg acts as an immune target against hepatocellular carcinoma associated with hepatitis B virus

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 164-170页

作者： Jun-Wen Yang,Dong-Ye Yang,fang-gen lu,Cai-Hong Li,Hui Chen,Ning Xie and Xin Zhao Department of Digestive Diseases,Second Xiangya Hospital,Central South University,Changsha 410011,China Department of Digestive Diseases Second Xiangya Hospital Central South University Changsha 410011 China

BACKGROUND:The incidence of hepatocellular carcinoma (HCC)in China is closely related to the population infected with hepatitis B virus(HBV).HCC cells with HBV secrete soluble HBsAg into blood but do not express it on the cell membrane This study aimed to construct and investigate a new glycosyl phosphatidylinositol(GPI)-anchored protein(GPC3+α+EGFP) as a DNA vaccine against HCC associated with HBV. METHODS:A recombinant plasmid(pcDNA3.1(+)/GPC3+ α+EGFP)was constructed and verified by restriction endo nuclease digestion and ***3.1(+)/GPC3+α+ EGFP was transfected into HepG2 cells(experimental group) using lipofectamine ***-N1-transfected HepG2 cells were used as a negative control,and non-transfected HepG2 cells sreved as a blank ***2 cells that steadily expressed the fusion protein GPC3+α+EGFP were screened by G418,propagated,and co-cultured with lymphocytes from healthy *** proliferation was measured by the classic sulforhodamine B *** was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL),and Fas gene transcription was determined by quantitative fluorescent PCR. RESULTS:The pcDNA3.1(+)/GPC3+α+EGFP plasmid was successfully *** the experimental group,green fluorescence was observed at the cell periphery and in the cytoplasm,whereas in the negative control group,fluorescence was evenly distributed throughout the *** of the experimental group significantly decreased after 72 hours compared to the negative and blank control ***,the number of apoptotic cells was statistically different among the three groups as determined by a contingency table Chisquare test;the experimental group had the highest incidence of *** gene transcription in the experimental group was higher than in the two control groups,and an increasing trend with time in the experimental group was observed. CONCluSION:A chimeric,membrane-anchored protein, GPC3+α+EGFP,localized

关键词： HBsAg-αepitope glypican 3 hepatocellular carcinoma hepatitis B virus protein engineering

Cross-subcarrier precoder design for massive MIMO-OFDM downlink with symplectic optimization

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Science China(Information Sciences) 2025年

作者： Yu-Xuan ZHANG An-An lu Xiang-gen XIA Xi-Qi GAO National Mobile Communications Research Laboratory Southeast University Purple Mountain Laboratories Department of Electrical and Computer Engineering University of Delaware

We propose a cross-subcarrier precoder design(CSPD) for massive multiple-input multiple-output(MIMO) orthogonal frequency division multiplexing(OFDM) downlink. This work aims to significantly improve the channel estimation and signal detection performance by enhancing the smoothness of the frequency domain effective channel. This is accomplished by designing a few vectors known as the transform domain precoding vectors(TDPVs), which are then transformed into the frequency domain to generate the precoders for a set of subcarriers. To combat the effect of channel aging, the TDPVs are optimized under imperfect channel state information(CSI). The optimal precoder structure is derived by maximizing an upper bound of the ergodic weighted sum-rate(WSR) and utilizing the a posteriori beam-based statistical channel model(BSCM). To avoid the large-dimensional matrix inversion, we propose an algorithm with symplectic optimization. Simulation results indicate that the proposed cross-subcarrier precoder design significantly outperforms conventional methods.

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