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Molecular evolution of hepatitis A virus in a human diploid cell line

World Journal of Gastroenterology 2007年第34期13卷 4630-4635页

作者： Cai-Hua Tang Jiang-Sen Mao Shao-Ai Chai Yong Chen fang-cheng zhuang Institute of Infectious Diseases the First Affiliated Hospital College of Medical Science Zhejiang UniversityHangzhou 310003 Zhejiang Province China Zhejiang Academy of Medical Science Hangzhou 310013 Zh-ejiang'Province China

AIM： To investigate the hoLspoLs, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells.METHODS： Wild type hepatitis A virus H2w was serially propagated in KMB17 cells until passage 30, and the fulllength genomes of H2w and its six chosen progenies were determined by directly sequencing RT-PCR products amplified from viral genomic RNA. Alignment comparison of sequences from H2w with its six progenies and phylogenetic analysis of the whole VP1 region from H2w, progenies of H2w, and other cell culture adapted hepatitis A virus were then carried out to obtain data on the molecular evolution of hepatitis A virus in the process of consecutive passage in KMB17 cells.RESULTS： Most of the mutations occurred by passage 5 and several hotspots related to adaptation of the virus during cell growth were observed. After that stage, few additional mutations occurred through the remaining duration of passage in KMB17 cells except for mutation in the virulence determinants, which occurred in the vicinity of passage 15. The phylogenetic analysis of the whole VP1 region suggested that the progenies of H2w evolved closely to other cell culture adapted hepatitis A virus, i.e. MBB, L-A-l, other than its progenitor H2w.CONCLUSION： Hepatitis A virus served as a useful model for studying molecular evolution of viruses in a given environment. The information obtained in this study may provide assistance in cultivating the next generation of a seed virus for live hepatitis A vaccine production.

关键词： Molecular evolution Virus evolution Phylogenyanalysis Virulence gene Hepat’s A virus

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Interfacial Zn2+-solvation regulator towards reversible and stable Zn anode

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Journal of Energy Chemistry 2025年第1期100卷 684-692页

作者： Miao Zhou Xiongbin Luo Hang Li Shan Guo zhuang Tong Xiaotao Zhou Xu Li Zhaohui Hou Shuquan Liang Guozhao fang College of Mechanical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional Materials Hunan Institute of Science and Technology School of Materials Science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South University College of Materials and Metallurgy Guizhou University National Energy Metal Resources and New Materials Key Laboratory Central South University

Aqueous zinc-ion batteries （AZIBs） are fundamentally challenged by the instability of the electrode/electrolyte interface,predominantly due to irreversible zinc （Zn） deposition and hydrogen ***,the intricate mechanisms behind the electrochemical discrepancies induced by interfacial Zn2+-solvation and deposition behavior demand comprehensive *** molecules endowed with special functional groups （such as hydroxyl,carboxyl,etc.） have the potential to significantly optimize the solvation structure of Zn2+and regulate the interfacial electric double layer （EDL）.By increasing nucleation overpotential and decreasing interfacial free energy,these functional groups facilitate a lower critical nucleation radius,thereby forming an asymptotic nucleation model to promote uniform Zn ***,this study presents a pioneering approach by introducing trace amounts of n-butanol as solvation regulators to engineer the homogenized Zn （H-Zn） anode with a uniform and dense *** interfacial reaction and structure evolution are explored by in/ex-situ experimental techniques,indicating that the H-Zn anode exhibits dendrite-free growth,no by-products,and weak hydrogen evolution,in sharp contrast to the bare ***,the H-Zn anode achieves a remarkable Zn utilization rate of approximately 20% and simultaneously sustains a prolonged cycle life exceeding 500 ***,the H-Zn//NH4V4O10（NVO） full battery showcases exceptional cycle stability,retaining 95.04%capacity retention after 400 cycles at a large current density of 5 A *** study enlightens solvation-regulated additives to develop Zn anode with superior utilization efficiency and extended operational lifespan.

关键词： Aqueous zinc-ion batteries Interfacial reaction Asymptotic nucleation model Reversible and stable Zn anode

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Lipid droplets in the nervous system:involvement in cell metabolic homeostasis

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Neural Regeneration Research 2025年第3期20卷 740-750页

作者： Yuchen Zhang Yiqing Chen cheng zhuang Jingxuan Qi Robert Chunhua Zhao Jiao Wang School of Life Sciences Shanghai UniversityShanghaiChina School of Medicine Shanghai UniversityShanghaiChina Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic MedicinePeking Union Medical CollegeBeijingChina Center of Excellence in Tissue Engineering Chinese Academy of Medical SciencesBeijingChina Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy BeijingChina

Lipid droplets serve as primary storage organelles for neutral lipids in neurons,glial cells,and other cells in the nervous *** droplet formation begins with the synthesis of neutral lipids in the endoplasmic ***,lipid droplets were recognized for their role in maintaining lipid metabolism and energy homeostasis;however,recent research has shown that lipid droplets are highly adaptive organelles with diverse functions in the nervous *** addition to their role in regulating cell metabolism,lipid droplets play a protective role in various cellular stress ***,lipid droplets exhibit specific functions in neurons and glial *** of lipid droplet formation leads to cellular dysfunction,metabolic abnormalities,and nervous system *** review aims to provide an overview of the role of lipid droplets in the nervous system,covering topics such as biogenesis,cellular specificity,and ***,it will explore the association between lipid droplets and neurodegenerative *** the involvement of lipid droplets in cell metabolic homeostasis related to the nervous system is crucial to determine the underlying causes and in exploring potential therapeutic approaches for these diseases.

关键词： Alzheimer's disease lipid droplet biogenesis lipid droplets lipid metabolism nervous system neurodegenerative disorders oxidative stress Parkinson's disease

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预制UHPC组合梁槽口式连接界面抗剪性能研究

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中国公路学报 2021年第2期34卷 276-288页

作者：姜海波董孝童邵腾飞方壮城广东工业大学土木与交通工程学院广东广州510006 甘肃省铁路投资建设集团有限公司甘肃兰州730000 东莞路桥投资建设有限公司广东东莞523000

相比现浇混凝土桥面板,全预制混凝土桥面板有诸多优势,能够提高桥梁工程质量、加快桥梁施工速度和降低成本。预制超高性能混凝土(Ultra-high Performance Concrete,UHPC)梁和预制UHPC桥面板通过槽口连接形成组合梁是一种新的结构形式,... 详细信息

相比现浇混凝土桥面板,全预制混凝土桥面板有诸多优势,能够提高桥梁工程质量、加快桥梁施工速度和降低成本。预制超高性能混凝土(Ultra-high Performance Concrete,UHPC)梁和预制UHPC桥面板通过槽口连接形成组合梁是一种新的结构形式,这种槽口式连接的界面抗剪性能会影响全梁整体承载力。通过16个推出试件,研究不同界面抗剪钢筋配筋率、预制梁混凝土类型和预制桥面板混凝土类型、槽口填充混凝土类型对界面抗剪承载力的影响,在试验过程中观测裂缝的发展和破坏模式,记录竖向滑移、水平滑移和试件破坏模式、钢筋应变、极限荷载Vu和残余荷载Vr。试验结果表明:界面抗剪钢筋配筋率对Vu和Vr起主要作用,配筋率为3.7%的界面极限荷载分别是配筋率为2.8%和2.0%的1.06倍、1.20倍;不同的槽内填充混凝土和预制梁混凝土二者共同影响Vu和Vr;预制桥面板混凝土类型对抗剪性能影响不大;钢筋的销栓作用主要受到钢筋直径和混凝土强度等级的影响;通过与AASHTO LRFD 2015和ACI 318规范对比发现,2个规范对UHPC组合梁槽口式连接界面抗剪承载力估计保守;提出的预制UHPC组合梁槽口式连接界面抗剪计算公式计算值与试验值吻合较好。

关键词：桥梁工程界面抗剪性能推出试验 UHPC组合梁槽口式连接

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预制节段干接缝体外预应力混凝土简支梁抗剪性能试验

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中国公路学报 2018年第12期31卷 188-195页

作者：姜海波李宇鸿肖杰陈远航方壮城广东工业大学土木与交通工程学院广东广州510006

预制节段干接缝体外预应力混凝土梁是一种适应于快速化施工的新型桥梁结构形式,然而预制节段干接缝体外预应力混凝土梁的斜截面抗剪破坏机理尚不明确。针对此类状况以文献[18]中推荐的箱型截面为原型,进行4根预制节段干接缝体外预应力... 详细信息

预制节段干接缝体外预应力混凝土梁是一种适应于快速化施工的新型桥梁结构形式,然而预制节段干接缝体外预应力混凝土梁的斜截面抗剪破坏机理尚不明确。针对此类状况以文献[18]中推荐的箱型截面为原型,进行4根预制节段干接缝体外预应力混凝土梁和3根整体式体外预应力混凝土梁的1∶8缩尺模型试验,揭示不同剪跨比(1.5,2.0和2.5)、接缝类型(整体式接缝和干接缝)以及接缝数量(2和4)对预制节段干接缝体外预应力混凝土梁斜截面抗剪性能的影响。在试验过程中观测裂缝的发展,记录体外束应力增量、挠度发展规律、接缝张开情况和破坏形态。试验结果表明:体外预应力预制节段干接缝混凝土梁在键齿处容易产生裂缝;剪跨比是影响节段梁和整体梁抗剪承载力的主要因素,随着剪跨比增大,节段梁和整体梁的抗剪承载力明显降低;在剪跨比小于或等于2.0时,预制节段干接缝体外预应力混凝土梁的抗剪承载力小于相应的整体式混凝土梁的抗剪承载力;根据节段式混凝土梁的接缝是否张开,节段式混凝土梁的受力过程可划分为接缝张开前、后2个阶段;在接缝张开前,节段式混凝土梁的力学行为与整体式混凝土梁的无异;接缝张开前、后,节段式混凝土梁的力学行为发生改变;接缝是控制梁抗剪承载力的主要因素,但接缝数量对节段式混凝土梁抗剪承载力的影响不显著。

关键词：桥梁工程斜截面抗剪性能模型试验预制节段混凝土梁干接缝体外束快速化施工

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基于改进虚拟多矢量的APF模型预测控制

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电力电子技术 2022年第10期56卷 133-136页

作者：张远史丽萍成方状韩丽中国矿业大学电气工程学院江苏徐州221008

针对有源电力滤波器(APF)的模型预测控制(MPC)策略,为提高APF对谐波的补偿精度,提出了一种基于改进虚拟多矢量模型预测APF控制方法。为降低模型预测每个周期内迭代计算的时间,提出改进扇区判断。通过改进扇区判断直接定位到输出矢量所... 详细信息

针对有源电力滤波器(APF)的模型预测控制(MPC)策略,为提高APF对谐波的补偿精度,提出了一种基于改进虚拟多矢量模型预测APF控制方法。为降低模型预测每个周期内迭代计算的时间,提出改进扇区判断。通过改进扇区判断直接定位到输出矢量所在的小扇区内,降低备选矢量的数量,降低运算周期和功率器件开关频率。同时,对模型预测的价值函数进行优化,使其更符合虚拟合成矢量参与后的评估。最后,通过仿真和实验对算法进行验证,所提算法不仅在补偿效果上有所提升,也缩短了迭代计算所需的时间。

关键词：有源电力滤波器模型预测控制虚拟多矢量

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Co/Co_(7)Fe_(3)heterostructures with controllable alloying degree on carbon spheres as bifunctional electrocatalyst forrechargeable zinc-air batteries

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 476-487页

作者： Junkang Chen Yongyue zhuang Yanxin Qiao Yu Zhang Aihua Yuan Hu Zhou School of Materials Science and Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China

Exploring efficient and nonprecious metal electrocatalysts of oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)is crucial for developing rechargeable zinc-air batteries(ZABs).Herein,an alloying-degree control strategy was employed to fabricate nitrogen-doped carbon sphere(NCS)decorated with dual-phase Co/Co_(7)Fe_(3)heterojunctions(CoFe@NCS).The phase composition of materials has been adjusted by controlling the alloying *** optimal CoFe_(0.08)@NCS electrocatalyst displays a half-wave potential of 0.80 V for ORR and an overpotential of 283 mV at 10 mA·cm^(-2)for OER in an alkaline *** intriguing bifunctional electrocatalytic activity and durability is attributed to the hierarchically porous structure and interfacial electron coupling of highly-active Co_(7)Fe_(3)alloy and metallic Co *** the CoFe_(0.08)@NCS material is used as air-cathode catalyst of rechargeable liquid-state zinc-air battery(ZAB),the device shows a high peak power-density(157 mW·cm^(-2))and maintains a stable voltage gap over 150 h,outperforming those of the benchmark(Pt/C+RuO_(2))-based *** particular,the as-fabricated solid-state flexible ZAB delivers a reliable compatibility under different bending *** work provides a promising strategy to develop metal/alloy-based electrocatalysts for the application in renewable energy conversion technologies.

关键词： bifunctional electrocatalysts oxygen reduction reaction oxygen evolution reaction zinc-air battery metal/alloy carbon sphere

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Role of the globus pallidus in motor and non-motor symptoms of Parkinson's disease

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Neural Regeneration Research 2025年第6期20卷 1628-1643页

作者： Yimiao Jiang Zengxin Qi Huixian Zhu Kangli Shen Ruiqi Liu Chenxin fang Weiwei Lou Yifan Jiang Wangrui Yuan Xin Cao Liang Chen Qianxing zhuang Department of Physiology School of MedicineNantong UniversityNantongJiangsu ProvinceChina Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghaiChina National Center for Neurological Disorders ShanghaiChina Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration ShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institute of Brain ScienceFudan UniversityShanghaiChina

The globus pallidus plays a pivotal role in the basal ganglia circuit. Parkinson's disease is characterized by degeneration of dopamine-producing cells in the substantia nigra, which leads to dopamine deficiency in the brain that subsequently manifests as various motor and non-motor symptoms. This review aims to summarize the involvement of the globus pallidus in both motor and non-motor manifestations of Parkinson's disease. The firing activities of parvalbumin neurons in the medial globus pallidus, including both the firing rate and pattern, exhibit strong correlations with the bradykinesia and rigidity associated with Parkinson's disease. Increased beta oscillations, which are highly correlated with bradykinesia and rigidity, are regulated by the lateral globus pallidus. Furthermore,bradykinesia and rigidity are strongly linked to the loss of dopaminergic projections within the cortical-basal ganglia-thalamocortical loop. Resting tremors are attributed to the transmission of pathological signals from the basal ganglia through the motor cortex to the cerebellum-ventral intermediate nucleus circuit. The cortico–striato–pallidal loop is responsible for mediating pallidi-associated sleep disorders. Medication and deep brain stimulation are the primary therapeutic strategies addressing the globus pallidus in Parkinson's disease. Medication is the primary treatment for motor symptoms in the early stages of Parkinson's disease, while deep brain stimulation has been clinically proven to be effective in alleviating symptoms in patients with advanced Parkinson's disease,particularly for the movement disorders caused by levodopa. Deep brain stimulation targeting the globus pallidus internus can improve motor function in patients with tremordominant and non-tremor-dominant Parkinson's disease, while deep brain stimulation targeting the globus pallidus externus can alter the temporal pattern of neural activity throughout the basal ganglia–thalamus network. Therefore, the comp

关键词： anxiety basal ganglia bradykinesia deep brain stimulation depression globus pallidus externus globus pallidus internus lateral globus pallidus medial globus pallidus neural circuit Parkinson's disease

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Effects of asymmetric rolling process on ridging resistance of ultra-purified 17%Cr ferritic stainless steel

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International Journal of Minerals,Metallurgy and Materials 2018年第2期25卷 216-225页

作者： cheng-zhuang Lu Jing-yuan Li Zhi fang School of Materials Science and Engineering University of Science and Technology Beijing Institute for Advanced Materials and Technology University of Science and Technology Beijing

In ferritic stainless steels, a significant non-uniform recrystallization orientation and a substantial texture gradient usually occur, which can degrade the ridging resistance of the final sheets. To improve the homogeneity of the recrystallization orientation and reduce the texture gradient in ultra-purified 17%Cr ferritic stainless steel, in this work, we performed conventional and asymmetric rolling processes and conducted macro and micro-texture analyses to investigate texture evolution under different cold-rolling conditions. In the conventional rolling specimens, we observed that the deformation was not uniform in the thickness direction, whereas there was homogeneous shear deformation in the asymmetric rolling specimens as well as the formation of uniform recrystallized grains and random orientation grains in the final annealing sheets. As such, the ridging resistance of the final sheets was significantly improved by employing the asymmetric rolling process. This result indicates with certainty that the texture gradient and orientation inhomogeneity can be attributed to non-uniform deformation, whereas the uniform orientation gradient in the thickness direction is explained by the increased number of shear bands obtained in the asymmetric rolling process.

关键词： ferritic stainless steel asymmetric rolling surface ridging texture shear bands

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Bidirectional regulation of the brain-gut-microbiota axis following traumatic brain injury

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Neural Regeneration Research 2025年第8期20卷 2153-2168页

作者： Xinyu You Lin Niu Jiafeng Fu Shining Ge Jiangwei Shi Yanjun Zhang Pengwei zhuang National Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese MedicineTianjinChina Haihe Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjinChina First Teaching Hospital of Tianjin University of Traditional Chinese Medicine TianjinChina National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion TianjinChina

Traumatic brain injury is a prevalent disorder of the central nervous *** addition to primary brain parenchymal damage,the enduring biological consequences of traumatic brain injury pose long-term risks for patients with traumatic brain injury;however,the underlying pathogenesis remains unclear,and effective intervention methods are *** dysfunction is a significant consequence of traumatic brain *** the most densely innervated peripheral tissue in the body,the gut possesses multiple pathways for the establishment of a bidirectional“brain-gut axis”with the central nervous *** gut harbors a vast microbial community,and alterations of the gut niche contribute to the progression of traumatic brain injury and its unfavorable prognosis through neuronal,hormonal,and immune pathways.A comprehensive understanding of microbiota-mediated peripheral neuroimmunomodulation mechanisms is needed to enhance treatment strategies for traumatic brain injury and its associated *** comprehensively reviewed alterations in the gut microecological environment following traumatic brain injury,with a specific focus on the complex biological processes of peripheral nerves,immunity,and microbes triggered by traumatic brain injury,encompassing autonomic dysfunction,neuroendocrine disturbances,peripheral immunosuppression,increased intestinal barrier permeability,compromised responses of sensory nerves to microorganisms,and potential effector nuclei in the central nervous system influenced by gut ***,we reviewed the mechanisms underlying secondary biological injury and the dynamic pathological responses that occur following injury to enhance our current understanding of how peripheral pathways impact the outcome of patients with traumatic brain *** review aimed to propose a conceptual model for future risk assessment of central nervous system-related diseases while elucidating novel insights into the bidirectional effec

关键词： traumatic brain injury brain-gut-microbiome axis gut microbiota neuroimmune immunosuppression host defense vagal afferents bacterial infection dorsal root ganglia nociception neural circuitry

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