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Mechanism of resveratrol on the promotion of induced pluripotent stem cells

Journal of Integrative Medicine 2013年第6期11卷 389-396页

作者： Dao-fang Ding Xiao-feng Li Hao Xu Zhen Wang Qian-qian Liang Chen-guang Li Yong-jun Wang Institute of Spine Shanghai University of Traditional Chinese Medicine Longhua Hospital Shanghai University of Traditional Chinese Medicine Laboratory of Cancer Hospital Fudan University

OBJECTIVE： To investigate the effects of resveratrol （RV） in reprogramming mouse embryonic fibroblasts （MEFs） into induced pluripotent stem cells （iPSCs） and the related mechanism. METHODS： Primary MEFs were isolated from E13.5 embryos and used within three passages. Retroviruses expressing Sox2 and Oct4 were produced by transfecting GP2-293t cells with recombinant plasmids murine stern cell virus （MSCV）-Sox2 and MSCV-Oct4. Supernatants containing retroviruses were obtained after 48-hour transfection and MEFs were then infected. Different concentrations （0, 5, 10 and 20 IJmol/L） of RV were added to embryonic stem cell （ESC） medium to culture MEFs 48 h post-infection, iPSC clones emerged and were further cultured. Expression of pluripotent markers of iPSCs was identified by cell immunofluorescence and reverse transcription-polymerase chain reaction. Both cytotoxicity and cell proliferation were assayed by Western blot analysis after RV was added into ESC medium. The ultrastructure change of mitochondria was observed by electron microscopy. RESULTS： More than 2.9-fold and 1.3-fold increases in colony number were observed by treatment with RV at 5 and 10 pmol/L, respectively. The reprogramming efficiency was significantly decreased by treatment with 20 pmol/L RV. The proliferation effect on MEFs or MEFs infected by two factors Sox2/Oct4 （2 factors-MEFs, 2F-MEFs） was investigated after RV treatment. At 20 pmol/L RV, induced cell apoptosis and proliferation inhibition were more obvious than those of 5 and 10 IJmol/L treatments. Clones were selected from the 10 pmol/L RV-treated group and cultured. Green fluorescent protein expression from one typical clone was silenced one month later which expressed ESC-associated marker genes Gdf3, Nanog, Ecatl, Fgf4 and Foxd3. Electron transmission microscope showed obvious cavitations in mitochondria. The expression of hypoxia-inducible factor-la was up-regulated when 2F-MEFs were treated with RV compared to the control group. CONClu

关键词： resveratrol plant extracts pluripotent stem cells hypoxia mitochondria cavitation in vitro

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Construction and Genetic Analysis of Murine Hepatitis Virus Strain A59 Nsp16 Temperature Sensitive Mutant and the Revertant Virus

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Virologica Sinica 2011年第1期26卷 19-29页

作者： Guo-hui Chang Bao-jun luo Pin lu Lei Lin Xiao-yan Wu Jing Li Yi Hu Qing-yu Zhu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China Changchun Changsheng Life Sciences Limited Changchun 130103 China

Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study,we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts18. Then we cultured the ts mutant Wu"-ts18(cd) at non-permissive temperature 39.5°C,which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts18 (cd) to non-ts phenotype,an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex.

关键词： Genetic analysis MHV-A59 Temperature-sensitive mutant Revertant Nonstructural proteins (Nsp)

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A nomogram predicting the recurrence of hepatocellular carcinoma in patients after laparoscopic hepatectomy

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Cancer Communications 2019年第1期39卷 499-509页

作者： Yang-Xun Pan Jian-Cong Chen Ai-Ping fang Xiao-Hui Wang Jin-Bin Chen jun-Cheng Wang Wei He Yi-Zhen Fu Li Xu Min-Shan Chen Yao-jun Zhang Qi-Jiong Li Zhong-Guo Zhou State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Liver Surgery Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Department of Public Health Sun Yat-sen UniversityGuangzhou 510080GuangdongP.R.China Department of Molecular Medicine California Campus The Scripps Research Institute10550North Torrey Pines RoadLa JollaCA 92037USA

Background:Patients with hepatocellular carcinoma(HCC)undergoing surgical resection still have a high 5-year recurrence rate(~60%).With the development of laparoscopic hepatectomy(LH),few studies have compared the efficacy between LH and traditional surgical approach on *** objective of this study was to establish a nomo-gram to evaluate the risk of recurrence in HCC patients who underwent ***:The clinical data of 432 patients,pathologically diagnosed with HCC,underwent LH as initial treatment and had surgical margin>1 cm were *** significance of their clinicopathological features to recurrence-free survival(RFS)was assessed,based on which a nomogram was constructed using a training cohort(n=324)and was internally validated using a temporal validation cohort(n=108).Results:Hepatitis B surface antigen(hazard ratio[HR],1.838;P=0.044),tumor number(HR,1.774;P=0.003),tumor thrombus(HR,2.356;P=0.003),cancer cell differentiation(HR,0.745;P=0.080),and microvascular tumor invasion(HR,1.673;P=0.007)were found to be independent risk factors for RFS in the training cohort,and were used for con-structing the *** C-index for RFS prediction in the training cohort using the nomogram was 0.786,which was higher than that of the 8th edition of the American Joint Committee on Cancer TNM classification(C-index,0.698)and the Barcelona Clinic Liver Cancer staging system(C-index,0.632).A high consistency between the nomogram prediction and actual observation was also demonstrated by a calibration *** improved predictive benefit in RFS and higher threshold probability of the nomogram were determined by receiver operating characteristic curve analysis,which was also confirmed in the validation cohort compared to other ***:We constructed and validated a nomogram able to quantify the risk of recurrence after initial LH for HCC patients,which can be clinically implemented in assisting the planification of individual postoperative surveil-lance

关键词： Hepatocellular carcinoma Laparoscopic hepatectomy Recurrence Nomogram American Joint Committee on Cancer TNM classification Barcelona Clinic Liver Cancer staging system Hepatitis B surface antigen Tumor thrombus Tumor invasion

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THE STUDY ON DOSE-AND TIME-EFFECT RELATIONSHIP OF YERENHE ON IMOROVING SLEEP

THE STUDY ON DOSE-AND TIME-EFFECT RELATIONSHIP OF YERENHE ON...

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中国睡眠研究会第十届全国学术年会

作者： JIN YANG lu jun Heilongjiang University Of Chinese Medicine

＜正＞Objective:To study the dose-and time-effect relationships of YERENHE on improving sleep. Methods:The ICR mice were selected as subjects,and the thecooperative method of Pentobarbital sodium with the underthresho...

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Effect of valsartan on the expression of angiotensin II receptors in the lung of chronic antigen exposure rats

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Chinese Medical Journal 2008年第22期121卷 2312-2319页

作者： WANG Tong YIN Kai-sheng LIU Kou-yin lu Guo-jun LI Yu-hua CHEN jun-di Department of Respiratory Medicine First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Department of Pathology of Nanjing Medical University Nanjing Jiangsu 210029 China

Background Many studies have suggested that angiotensin Ⅱ （Ang Ⅱ） and its receptors may be involved in the development of asthma. However, the expression of angiotensin Ⅱ receptors （AGTR） is not clear in the lung tissue of chronic asthmatics. This study was designed to determine the relationship between airway remodeling, dysfunction and the expression of AGTRs in a rat model of asthma. Methods Rats were sensitized with ovalbumin （OVA） for 2 weeks. Sixty minutes before an inhalation challenge, the rats were pretreated either with valsartan （15, 30, 50 ***-1.d-1） or saline intragastrically. Then the rats received an OVA challenge for 30 alternative days. Acetylcholine （Ach）-induced bronchoconstriction was measured after the final antigen challenge. White cell counts in bronchoalveolar lavage fluid （BALF） and morphological changes in the airways were then assessed. The levels of transforming growth factor-beta 1 （TGF-β1） and platelet-derived growth factor （PDGF） in BALF were detected by ELISA. The levels of AGTR1 and AGTR2 mRNA and protein in lung tissues were measured by RT-PCR and Western blotting. Results AGTR1 mRNA and protein levels in repeatedly OVA-challenged rats were significantly increased as compared with negative controls. The AGTR1 mRNA expression versus white cell counts of BALF and airway wall thickness （mainly in small airways） in lungs of chronic antigen-exposed rats were positively correlated. Valsartan decreased the level of AGTR1 in repeatedly OVA-challenged rats. However, AGTR2 mRNA and protein levels in the OVA-challenged rats and high-dose valsartan-treated rats （50 ***-1.d-1） were also increased. Valsartan significantly decreased inflammatory cell accumulation and attenuated Ach-evoked bronchoconstriction in repeatedly antigen-challenged rats. Valsartan also decreased allergen-induced structural changes in rat airway （including total airway wall thickness and smooth muscle area） and the levels of TGF-β1 and PDGF in BALE Conclusions AGTR1 ex

关键词： asthma angiotensin Ⅱ receptor airway inflammation airway remodeling angiotensin H receptor antagonist

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Towards extreme fast charging of 4.6 V LiCoO_(2) via mitigating high-voltage kinetic hindrance

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Journal of Energy Chemistry 2023年第3期78卷 13-20,I0001页

作者： Yu Tang jun Zhao He Zhu Jincan Ren Wei Wang Yongjin fang Zhiyong Huang Zijia Yin Yalan Huang Binghao Zhang Tingting Yang Tianyi Li Leighanne CGallington Si Lan Yang Ren Qi Liu Department of Physics City University of Hong KongHong Kong 999077China Department of Materials Science and Engineering City University of Hong KongHong Kong 999077China Shenzhen Research Institute City University of Hong KongShenzhen 518057GuangdongChina Argonne National Laboratory X-Ray Science DivisionArgonneIL 60439USA

High-voltage LiCoO_(2)(LCO) is an attractive cathode for ultra-high energy density lithium-ion batteries(LIBs) in the 3 C ***,the sluggish lithium-ion diffusion at high voltage significantly hampers its rate ***,combining experiments with density functional theory(DFT) calculations,we demonstrate that the kinetic limitations can be mitigated by a facial Mg^(2+)+Gd^(3+)co-doping *** as-prepared LCO shows significantly enhanced Li-ion diffusion mobility at high voltage,making more homogenous Li-ion de/intercalation at a high-rate charge/discharge *** homogeneity enables the structural stability of LCO at a high-rate current density,inhibiting stress accumulation and irreversible phase *** used in combination with a Li metal anode,the doped LCO shows an extreme fast charging(XFC) capability,with a superior high capacity of 193.1 mAh g^(-1)even at the current density of 20 C and high-rate capacity retention of 91.3% after 100 cycles at 5 *** work provides a new insight to prepare XFC high-voltage LCO cathode materials.

关键词： Li-ion battery High-voltage LiCoO_(2) Li-ion diffusion Structural evolution Fast charging

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A Communication Theory Perspective on Prompting Engineering Methods for Large Language Models

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Journal of Computer Science & Technology 2024年第4期39卷 984-1004页

作者： Yuan-Feng Song Yuan-Qin He Xue-fang Zhao Han-Lin Gu Di Jiang Hai-jun Yang Li-Xin Fan AI Group WeBank Co.LtdShenzhen 518000China

The springing up of large language models(LLMs)has shifted the community from single-task-orientated natural language processing(NLP)research to a holistic end-to-end multi-task learning *** this line of research endeavors in the area,LLM-based prompting methods have attracted much attention,partially due to the technological advantages brought by prompt engineering(PE)as well as the underlying NLP principles disclosed by various prompting *** supervised learning usually requires training a model based on labeled data and then making *** contrast,PE methods directly use the powerful capabilities of existing LLMs(e.g.,GPT-3 and GPT-4)via composing appropriate prompts,especially under few-shot or zero-shot *** the abundance of studies related to the prompting and the ever-evolving nature of this field,this article aims to 1)illustrate a novel perspective to review existing PE methods within the well-established communication theory framework,2)facilitate a better/deeper understanding of developing trends of existing PE methods used in three typical tasks,and 3)shed light on promising research directions for future PE methods.

关键词： prompting method large language model communication theory

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Critical influenza （H1N1） pneumonia： imaging manifestations and histopathological findings

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Chinese Medical Journal 2012年第12期125卷 2109-2114页

作者： LI Hong-jun CHENG Jing-liang LINing LI Yun-fang ZHANG Hui-mao Beijing Youan Hospital Capital Medical University Beijing100069 China First Hospital Zhengzhou University Zhengzhou Henan 450052China Department of Diagnostic Radiology China Japan Union HospitalJilin University Jilin Jilin 132000 China

Background The global outbreak of influenza A （H1N1） has led to the Ministry of Health of China listing it as one of the A-class infectious diseases. Pneumonia is the most serious complication of influenza A, commonly causing death. Populations are ordinarily susceptible to influenza A. This study aimed to investigate the imaging manifestation features of critical influenza A （H1 N1 ） pneumonia and to improve its diagnostic techniques. Methods A total of seven death cases from critical influenza A （H1N1） pneumonia were retrospectively analyzed on their imaging manifestations and autopsy data. Pulmonary CT scanning was performed for five cases, with one receiving additional chest X-ray and chest CT scanning, and chest postero-anterior position X-ray examination was performed for other two. Autopsy was performed for five cases and postmortem examinations were performed for other two cases. Results The seven cases of influenza A showed critical manifestations in 4-7 days after symptoms onset, with two having basic diseases of diabetes and one being pregnant. Extensive blurry high-density shadows of bilateral lungs were found in three cases, which were most obvious in middle and inferior parts of lungs. Pulmonary CT scanning revealed bilateral flaky parenchymal shadows in peripheral, dorsal and fundus segments of the middle-inferior parts of lungs, with one case of complicated pneumothorax, atelectasis and pleural effusion and another case of thin-walled cavity and dilated bronchi shadows in the superior parts of lungs. Conclusions Diagnostic imaging is an important assessing tool for critical influenza A （H1N1） pneumonia. The imaging manifestations are characteristic instead of being specific. The definitive diagnosis can be made in combination with clinical examinations and laboratory tests.

关键词： critical diseases type A influenza imaging manifestation pneumonia

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Correlation between serum markers and transjugular intrahepatic portosystemic shunt prognosis in patients with cirrhotic ascites

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World Journal of Gastrointestinal Surgery 2024年第2期16卷 481-490页

作者： Xiao-Gang Hu Xiao-Xian Yang jun lu Gang Li Jian-Ji Dai Jia-Min Wang Yi Deng Rui Feng Department of Interventional Radiology The Affiliated Jinhua HospitalZhejiang University School of MedicineJinhua 321000Zhejiang ProvinceChina Medical College Jinhua PolytechnicJinhua 321017Zhejiang ProvinceChina Department of Interventional Medicine Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Individuals with refractory ascites in the context of liver cirrhosis typically face an adverse *** transjugular intrahepatic portosystemic shunt(TIPS)is an efficacious intervention,but there is a lack of reliable tools for postoperative pro-gnosis *** utilized clinical biochemical markers,such as the serum albumin concentration(Alb),sodium(Na+)concentration,and serum creatinine(Scr),have limited predictive ***,the quest for novel,specific biomarkers to evaluate the post-TIPS prognosis in patients with liver cirrhosis and refractory ascites holds significant practical importance.A retrospective analysis was conducted on 75 patients with liver cirrhosis and refractory ascites who underwent TIPS at our institution from August 2019 to August *** patients were followed up regularly for two years,and the death toll was meticulously *** patients were allocated into a survival group(n=45 patients)or a deceased group(n=30 patients)based on their prognosis *** clinical data of the two groups were collected,and Child-Pugh scores and MELD scores were calculated for *** correlation analysis was carried out to evaluate the correlation of prognosis with Child-Pugh grade,MELD score,and Cys C ***,a multiple-factor analysis utilizing the Cox proportional hazard model was used to identify independent risk factors affecting the post-TIPS prognosis of patients with liver cirrhosis and refractory *** receiver operating characteristic curve(ROC)ascertained the predictive value of the Cys C concen-tration,Child-Pugh grade,and MELD score for the prognosis of liver cirrhosis with refractory ascites in post-TIPS *** During a 2-year follow-up period,among 75 patients with liver cirrhosis and refractory ascites who underwent TIPS treatment,30 patients(40.00%)passed *** deceased cohort exhibited heightened aspartate aminotrans-ferase,alanine aminotransferase,total b

关键词： Liver cirrhosis Refractory ascites Transjugular intrahepatic portosystemic shunt Cystatin C

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纳米碳酸钙增容PVC/LDPE共混体系的研究

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高分子材料科学与工程 2005年第6期21卷 251-253,257页

作者：张璐佟立芳方征平浙江大学高分子复合材料研究所浙江杭州310027

采用经过表面处理的纳米碳酸钙作为相容剂,制备了PVC/LDPE/纳米碳酸钙的共混物,对其进行了DSC、DM A测试,并用相差显微镜观察了共混物的形态。研究结果表明,纳米碳酸钙的加入影响了共混物中LDPE的结晶性能,降低了PVC的玻璃化转变温度,... 详细信息

采用经过表面处理的纳米碳酸钙作为相容剂,制备了PVC/LDPE/纳米碳酸钙的共混物,对其进行了DSC、DM A测试,并用相差显微镜观察了共混物的形态。研究结果表明,纳米碳酸钙的加入影响了共混物中LDPE的结晶性能,降低了PVC的玻璃化转变温度,减小了分散相尺寸。说明纳米碳酸钙能有效地提高PVC/LDPE的相容性。

关键词：纳米碳酸钙聚氯乙烯聚乙烯共混相容性

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