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Molecular evolution of hepatitis A virus in a human diploid cell line

World Journal of Gastroenterology 2007年第34期13卷 4630-4635页

作者： Cai-Hua Tang Jiang-Sen Mao Shao-Ai Chai Yong Chen fang-Cheng zhuang Institute of Infectious Diseases the First Affiliated Hospital College of Medical Science Zhejiang UniversityHangzhou 310003 Zhejiang Province China Zhejiang Academy of Medical Science Hangzhou 310013 Zh-ejiang'Province China

AIM： To investigate the hoLspoLs, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells.METHODS： Wild type hepatitis A virus H2w was serially propagated in KMB17 cells until passage 30, and the fulllength genomes of H2w and its six chosen progenies were determined by directly sequencing RT-PCR products amplified from viral genomic RNA. Alignment comparison of sequences from H2w with its six progenies and phylogenetic analysis of the whole VP1 region from H2w, progenies of H2w, and other cell culture adapted hepatitis A virus were then carried out to obtain data on the molecular evolution of hepatitis A virus in the process of consecutive passage in KMB17 cells.RESULTS： Most of the mutations occurred by passage 5 and several hotspots related to adaptation of the virus during cell growth were observed. After that stage, few additional mutations occurred through the remaining duration of passage in KMB17 cells except for mutation in the virulence determinants, which occurred in the vicinity of passage 15. The phylogenetic analysis of the whole VP1 region suggested that the progenies of H2w evolved closely to other cell culture adapted hepatitis A virus, i.e. MBB, L-A-l, other than its progenitor H2w.CONCLUSION： Hepatitis A virus served as a useful model for studying molecular evolution of viruses in a given environment. The information obtained in this study may provide assistance in cultivating the next generation of a seed virus for live hepatitis A vaccine production.

关键词： Molecular evolution Virus evolution Phylogenyanalysis Virulence gene Hepat’s A virus

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Electrical properties of garnet-like lithium ionic conductors Li5＋xSrxLa3-xBi2O12 fabricated by spark plasma sintering method

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Frontiers of Materials Science 2012年第3期6卷 216-224页

作者： Yun-Xia GAO Xian-Ping WANG Qin-Xing SUN Zhong zhuang Qian-Feng fang Key Laboratory of Materials Physics Institute of Solid State PhysicsChinese Academy of SciencesHefei 230031China

A typical approach involving Pechini method and spark plasma sintering （SPS） method was presented for the preparation of high density Li5＋xSrxLa3_xBi2012 （x = 0, 1） ceramics. Phase formation, microstructure, grain size and electrical properties of the specimens were examined using XRD, SEM and alternating current impedance spectro- scopy （ACIS）. Dense LisLa3Bi2012 and LisSrLa2Bi2012 ceramics with pure garnet-like phase, relative density of 97% and average grain size of about 5 pm were fabricated using this approach. The total conductivities at 298 K of Li5La3Bi2012 and LisSrLa2Bi2O12 ceramics prepared by the SPS method are 5.1 Qian-Feng fang105 and 6.810SS/cm, respectively, 2 times higher than that of samples prepared by the conventional sintering method.

关键词： lithium ionic conductor Lis.xSrxLa3_xBi2012 Pechini method spark plasmasintering (SPS) ionic conductivity

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Percutaneous coronary intervention for ostial lesions of the left main stem in a patient with congenital single left coronary artery: A case report

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World Journal of Clinical Cases 2019年第15期7卷 2128-2133页

作者： Qiang Wu Zong-zhuang Li Feng Yue fang Wei Chen-Yun Zhang Department of Cardiology Guizhou Provincial People’s Hospital

BACKGROUND Single coronary artery(SCA)originating from a solitary ostium in the aorta and perfusing the entire myocardium is a very rare congenital anomaly of the coronary ***,a right coronary artery(RCA)arising from the mid segment of the left anterior descending artery(LAD)is an extremely uncommon variation of *** SUMMARY A 76-year-old woman presented a 5-mo history of exertional *** coronary angiography revealed an SCA,with severe ostial stenosis that originated from the left sinus of Valsalva and bifurcated normally into the LAD and circumflex coronary *** addition,an anomalous RCA originated from the mid segment of the LAD as a separate *** balloon angioplasty and stenting for the SCA ostial stenosis were performed on the *** Percutaneous coronary intervention(PCI)of the main trunk for SCA is very similar to PCI of an unprotected left main coronary *** technical difficulties and risks do exist,PCI for severe ostial stenosis of the main trunk is safe and efficacious in selected SCA patients.

关键词： Coronary anomaly Single coronary artery Left main coronary artery Percutaneous coronary intervention Case report

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Neuroprotective effect of recombinant human erythropoietin on optic nerve injury in rats

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Chinese Medical Journal 2009年第17期122卷 2008-2012页

作者： WANG Hui LIU Zhe-li zhuang Xiao-tong WANG Min-fang XU Li Department of Ophthalmology First Hospital of China MedicalUniversity Shenyang Liaoning 110001 China Department of Ophthalmology Forth Republic Hospital ofShenyang Shenyang Liaoning 110031 China

Background Optic nerve injury, caused by retinal and optic nerve diseases, can eventually result in vision loss. To date, few effective treatments have been discovered to restore visual function. Previous studies showed that recombinant human erythropoietin （rhEPO） has a neuroprotective effect on the central nervous system, particularly in nerve injury. In this study, we investigated the effects of rhEPO on axonal regeneration and functional restoration following optic nerve injury. This was done by measuring the expression of growth associated protein 43 （GAP-43）, a marker for neuronal regeneration, on the retina and flash-visual evoked potential （F-VEP）. Methods Adult Wistar rats were randomly assigned to rhEPO and control （saline） groups. Optic nerve crush injury models were established and rhEPO or saline were immediately injected into the vitreous cavity. The expression of GAP-43 was detected by immunohistochemistry and the F-VEP was measured pre-injury, immediately after injury, 1 week and 2 weeks post-injury. Results No detectable staining for GAP-43 was observed in normal retina. In the control group, the level of GAP-43 expression was higher at 1 week post-injury, but decreased at 2 weeks. In the rhEPO group, the level of GAP-43 expression was notably higher at both 1 week and 2 weeks. At each time point post-injury, the expression of GAP-43 in rhEPO group was significantly higher than the control group （P 〈0.05）. Obvious changes in F-VEP examination were detected immediately after optic nerve injury, including significantly prolonged latency and decreased amplitude of the P1 wave. In the control group, the changes were still obvious at 1 week. The latency was decreased and the amplitude had slightly recovered to 28.23% of the normal value at 2 weeks. In rhEPO group, there was significantly more recovery than the control group at 1 week and 2 weeks post-injury （P 〈0.05）. The latency most close to the normal level and the amplitude had recovered to 65.51% of

关键词： erythropoietin optic nerve injuries," retinal ganglion cells," growth associated protein 43

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Intra-abdominal inflammatory pseudotumor-like follicular dendritic cell sarcoma associated with paraneoplastic pemphigus: A case report and review of the literature

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World Journal of Clinical Cases 2020年第14期8卷 3097-3107页

作者： Jia-Yi zhuang fang-Fei Zhang Qing-Wen Li Yong-Feng Chen Department of Dermatology Dermatology HospitalSouthern Medical University/Guangdong Provincial Dermatology HospitalGuangzhou 510091Guangdong ProvinceChina

BACKGROUD Follicular dendritic cell(FDC)sarcomas are rare neoplasms that occur predominantly in the lymph *** can also occur *** FDC sarcomas most commonly present as solitary *** pseudotumor(IPT)-like FDC sarcomas,a subcategory of FDC sarcomas,are rarer than other sarcoma *** are composed of spindle or ovoid neoplastic cells and exhibit an admixture of plasma cells and prominent lymphoplasmacytic *** pemphigus(PNP),also known as paraneoplastic autoimmune multiorgan syndrome,is a rare autoimmune bullous disease that is associated with underlying *** has a high mortality,and its early diagnosis is usually *** SUMMARY We describe a 27-year-old woman who presented with stomatitis,conjunctivitis,and skin blisters and erosions as her first symptoms of PNP with an intraabdominal IPT-like FDC *** patient underwent surgical tumor resection and received tapering oral corticosteroid *** showed no recurrence at the 1-year *** IPT-like FDC sarcomas are rare underlying neoplasms that have an uncommon association with ***-associated FDC sarcomas predominantly occur in intra-abdominal sites and suggest a poor *** resection is an essential and effective treatment for PNP and primary and recurrent FDC sarcomas.

关键词： Inflammatory pseudotumor-like follicular dendritic cell sarcoma Paraneoplastic pemphigus Follicular dendritic cell sarcoma Resection Case report

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Investigation of Intrachain Exciton Diffusion of MEH-PPV in Solution with Different Polarity

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Chinese Journal of Chemical Physics 2019年第2期32卷 273-276,I0003页

作者： Xue-cong Li Qi-lin Yuan fang Zhang Han-zhuang Zhang Department of Physics Tianjin Polytechnic University TianJin 300387 China Femtosecond laser laboratory College of Physics Jilin University Changchun 130012 China

Femtosecond time-resolved transient grating technique was adopted to insight into the intra-chain exciton diffusion of MEH-PPV in solution with different polarity. Broadband white-light continuum was introduced as the probe to observe the transient absorption and the femtosecond time-resolved transient grating information simultaneously. The vibrational dephasing behaviors, single exciton relaxation, and population relaxation dynamics of MEH-PPV were systematically investigated. The result shows that the relaxation processes of the sample solution will be accelerated in the solvent with larger polarity.

关键词： Transient grating Exciton diffusion Poly[2-methoxy-5-(2'-ethylhexoxy)]-1,4-phenylene vinylene

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新时代背景下民族体育异化与否的判断审视与利弊探讨

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当代体育科技 2024年第1期14卷 106-109页

作者：李凤方萍河池学院广西河池546300

异化是民族体育在适应时代发展的过程中不可回避的问题。从界定民族体育异化概念出发,提出异化的判断标准并进行价值审视,指出应依据社会生活的变迁与发展来客观理解和面对,依据民族体育的属性和价值来理性分析和认识,同时,结合当前民... 详细信息

异化是民族体育在适应时代发展的过程中不可回避的问题。从界定民族体育异化概念出发,提出异化的判断标准并进行价值审视,指出应依据社会生活的变迁与发展来客观理解和面对,依据民族体育的属性和价值来理性分析和认识,同时,结合当前民族体育异化的几种主要表现形式展开分析,探讨利弊,辩证看待异化的积极与消极方面。正视异化现象,与时俱进,正确引导;科学管理,依法治体,扬长避短,促进民族体育健康发展,以更好地服务全民健身国家战略。

关键词：民族体育异化价值判断利弊

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大苞苣苔属(苦苣苔科)一新组合及一新异名

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广西植物 2024年第09期44卷 1641-1648页

作者：熊小凯顾江淼温放白新祥贵州大学林学院广西壮族自治区中国科学院广西植物研究所广西喀斯特植物保育与恢复生态学重点实验室中国野生植物保护协会苦苣苔专业委员会国家苦苣苔科种质资源库GXIB中国苦苣苔科植物保育中心

大苞苣苔属(Anna Pellegr.)自1930年建立以来，学者对该属植物的分类修订甚少，早期的物种发表和修订都依据采集的标本完成，存在性状描述简略或不准确、物种界定模糊等问题。在对白花大苞苣苔[Anna ophiorrhizoides(Hemsl.) B. L. Burt... 详细信息

大苞苣苔属(Anna Pellegr.)自1930年建立以来，学者对该属植物的分类修订甚少，早期的物种发表和修订都依据采集的标本完成，存在性状描述简略或不准确、物种界定模糊等问题。在对白花大苞苣苔[Anna ophiorrhizoides(Hemsl.) B. L. Burtt&R. A. Davidson]和红花大苞苣苔(*** S. Z. He, F. Wen&Y. G. Wei)的历史考证、形态学对比和模式产地的调查考证中发现，1911年H. Léveillé发表的Didymocarpus cavaleriei H. Lév.与红花大苞苣苔实为同种，前者被处理为白花大苞苣苔的异名。在对白花大苞苣苔和红花大苞苣苔的多个居群形态特征对比和地理分布信息整理后发现，二者的形态特征有一定的差异，但仅依据花冠颜色作为种一级分类单位的界限并不恰当，综合考虑形态和地理分布特征，故提出将红花大苞苣苔处理为白花大苞苣苔的变种。依据2018年International Code of Nomenclature for Algae,Fungi,and Plants(Shenzhen Code)中的规定和建议，该文提出一个新组合兼新等级名称Anna ophiorrhizoides(Hemsl.) B. L. Burtt&R. A. Davidson ***(H. Lév.)X. X. Bai&F. Wen,并将红花大苞苣苔处理为新组合的异名。

关键词：大苞苣苔属红花大苞苣苔白花大苞苣苔新组合新异名

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Clinical manifestation,lifestyle,and treatment patterns of chronic erosive gastritis:A multicenter real-world study in China

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World Journal of Gastroenterology 2024年第9期30卷 1108-1120页

作者： Ying-Yun Yang Ke-Min Li Gui-fang Xu Cheng-Dang Wang Hua Xiong Xiao-Zhong Wang Chun-Hui Wang Bing-Yong Zhang Hai-Xing Jiang Jing Sun Yan Xu Li-Juan Zhang Hao-Xuan Zheng Xiang-Bin Xing Liang-Jing Wang Xiu-Li Zuo Shi-Gang Ding Rong Lin Chun-Xiao Chen Xing-Wei Wang Jing-Nan Li Department of Gastroenterology Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China Department of Gastroenterology Nanjing Drum Tower HospitalNanjing 21000Jiangsu ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Gastroenterology Renji HospitalShanghai 200127China Department of Gastroenterology Union Hospital of Fujian Medical UniversityFuzhou 350000Fujian ProvinceChina Department of Gastroenterology West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Gastroenterology and Hepatology The People’s Hospital of Zhengzhou UniversityThe Henan Provincial People’s HospitalZhengzhou 450003Henan ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai Jiao Tong UnivesrityRuijin HospitalSchool MedicineShanghai 200025China Department of Gastroenterology and Hepatology China-Japan Union Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina Department of Gastroenterology Fujian Medical University Union HospitalFuzhou 350001Fujian ProvinceChina Department of Gastroenterology Nanfang HospitalGuangzhou 510080Guangdong ProvinceChina Department of Gastroenterology The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina Department of Gastroenterology The Second Affiliated Hospital Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Gastroenterology Shandong University Qilu HospitalJinan 250012Shandong ProvinceChina Department of Gastroenterology Peking University Third HospitalBeijing 100191China Department of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei Pr

BACKGROUND Although chronic erosive gastritis(CEG)is common,its clinical characteristics have not been fully *** lack of consensus regarding its treatment has resulted in varied treatment *** To explore the clinical characteristics,treatment patterns,and short-term outcomes in CEG patients in *** We recruited patients with chronic non-atrophic or mild-to-moderate atrophic gastritis with erosion based on endoscopy and *** and treating physicians completed a questionnaire regarding history,endoscopic findings,and treatment plans as well as a follow-up questionnaire to investigate changes in symptoms after 4 wk of *** Three thousand five hundred sixty-three patients from 42 centers across 24 cities in China were *** pain(68.0%),abdominal distension(62.6%),and postprandial fullness(47.5%)were the most common presenting *** was classified as chronic non-atrophic in 69.9%of *** those with erosive lesions,72.1%of patients had lesions in the antrum,51.0%had multiple lesions,and 67.3%had superficial flat *** patients with epigastric pain,the combination of a mucosal protective agent(MPA)and proton pump inhibitor was more *** those with postprandial fullness,acid regurgitation,early satiety,or nausea,a MPA appeared more *** CEG is a multifactorial disease which is common in Asian patients and has non-specific *** may play a major role in its detection and *** should be individualized based on symptom profile.

关键词： Chronic erosive gastritis Symptom Endoscopic findings Treatment pattern Real-world

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不作为型容留他人吸毒的认定路径与规则--以莫某某容留他人吸毒案为切入点

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医学与法学 2024年第5期16卷 99-106页

作者：柳安然张芳北京中医药大学北京朝阳102401 广西壮族自治区高级人民法院广西南宁412000

我国已将不作为型容留他人吸毒司法犯罪化,这不违反罪刑法定原则,但应注意防止该罪在司法适用中的不当扩张;认定该罪的不真正不作为犯,关键在于确定不作为的作为义务来源。对作为义务的判断不能只停留在形式层面,还应对其进行等价性实... 详细信息

我国已将不作为型容留他人吸毒司法犯罪化,这不违反罪刑法定原则,但应注意防止该罪在司法适用中的不当扩张;认定该罪的不真正不作为犯,关键在于确定不作为的作为义务来源。对作为义务的判断不能只停留在形式层面,还应对其进行等价性实质判断;只有与该罪的作为行为具有同等违法价值的不作为行为才构成犯罪。不作为型容留他人吸毒构成犯罪的认定规则,应以不同行为主体为区分;只有对吸毒场所具有绝对的、现实的、排他性支配地位的主体,在未履行法律法规、职业、经营等所要求的制止、举报义务时,才能成立容留他人吸毒罪的不真正不作为犯。

关键词：容留他人吸毒罪不真正不作为作为义务同等违法价值

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