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The genome of the recretohalophyte Limonium bicolor provides insights into salt gland development and salinity adaptation during terrestrial evolution

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Molecular Plant 2022年第6期15卷 1024-1044页

作者： fang Yuan Xi Wang Boqing Zhao Xiaojing Xu Miao Shi Bingying leng Xinxiu Dong Chaoxia Lu Zhongtao Feng Jianrong Guo Guoliang Han Haikuan Zhang Jianwei Huang Min Chen Bao-Shan Wang Shandong Provincial Key Laboratory of Plant Stress College of Life SciencesShandong Normal UniversityJi’nanShandongChina Berry Genomics Corporation BeijingChina

Halophytes have evolved specialized strategies to cope with high *** extreme halophyte sea lavender(Limonium bicolor)lacks trichomes but possesses salt glands on its epidermis that can excrete harmful ions,such as sodium,to avoid salt ***,we report a high-quality,2.92-Gb,chromosome-scale *** genome assembly based on a combination of Illumina short reads,single-molecule,real-time long reads,chromosome conformation capture(Hi-C)data,and Bionano genome maps,greatly enriching the genomic information on recretohalophytes with multicellular salt *** the *** genome contains genes that show similarity to trichome fate genes from Arabidopsis thaliana,it lacks homologs of the decision fate genes GLABRA3,ENHANCER OF GLABRA3,GLABRA2,TRANSPARENT TESTA GLABRA2,and SIAMESE,providing a molecular explanation for the absence of trichomes in this *** identified key genes(LbHLH and LbTTG1)controlling salt gland development among classical trichome homologous genes and confirmed their roles by showing that their mutations markedly disrupted salt gland initiation,salt secretion,and salt tolerance,thus offering genetic support for the long-standing hypothesis that salt glands and trichomes may share a common *** addition,a whole-genome duplication event occurred in the *** genome after its divergence from Tartary buckwheat and may have contributed to its adaptation to high *** *** genome resource and genetic evidence reported in this study provide profound insights into plant salt tolerance mechanisms that may facilitate the engineering of salt-tolerant crops.

关键词： development evolution genome Limonium bicolor salinity adaptation salt gland

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随机结构地震响应的TMD控制

西北工业大学学报 2007年第2期25卷 311-314页

作者：吴存利冷小磊方同西北工业大学力学与土木建筑学院陕西西安710072 南京航空航天大学振动工程研究所南京210016

讨论了随机结构地震响应的TMD控制及其优化问题。借Gegenbauer多项式逼近法,首先把随机结构-TMD系统转化为等效的确定性扩阶系统,然后用演变随机响应统一解法求其随机响应。引进响应面法来建立结构随机响应与TMD参数之间的近似关系式,... 详细信息

讨论了随机结构地震响应的TMD控制及其优化问题。借Gegenbauer多项式逼近法,首先把随机结构-TMD系统转化为等效的确定性扩阶系统,然后用演变随机响应统一解法求其随机响应。引进响应面法来建立结构随机响应与TMD参数之间的近似关系式,通过优化得到随机结构响应的最优TMD控制系统。算例证实,最优TMD可最有效地控制结构的总体响应,且对随机结构的每一个样本都有很好的控制效果。所以说,最优TMD控制具有鲁棒性。

关键词：随机结构 Gegenbauer多项式近似 TMD

Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L.

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Chinese Science Bulletin 2011年第23期56卷 2437-2443页

作者： YANG YaoLong RAO YuChun LIU HuiJuan fang YunXia DONG GuoJun HUANG LiChao leng YuJia GUO LonBiao ZHANG GuangHeng HU Jiang GAO ZhenYu QIAN Qian ZENG DaLi State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China College of Life and Environment Sciences Hangzhou Normal University Hangzhou 310036 China

An es-t (early senescence-temporary) mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identified in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffin section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromosome 5. These results will facilitate the positional cloning and functional studies of the gene.

关键词： ES 衰老早期精细定位突变水稻扫描电子显微镜表征

Synthesis and Crystal Structure of Diethyl (4,6-diamino-1,3,5-triazine-2-yl) phosphonate

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Chinese Journal of Structural Chemistry 2000年第4期19卷 244-248页

作者： HU fang Zhong ZOU Xiao Mao YANG Hua Zheng * WENG Lin Hong leng Xue Bing (State Key Laboratory of Elemento Organic Chemistry, Institute of Elemento Organic Chemistry, Nankai University, Tianjin 300071) 南开大学元素有机化学国家重点实验室天津 30 0 0 71

The crystal of the title compound diethyl (4,6 diamino 1,3,5 triazine 2 yl) phosphonate, (C 14H 28N 10O 6P 2, M r =494.40), has been prepared and determined by X ray single crystal diffraction. The crystal belongs to the triclinic system, space group P 1 with parameters: a=8.398(1) , b=10.206(1), c=14.538(2), α=75.221(3), β=85.965(3), γ=81.746(3)°, V=1191.6(3) 3, S=1.028, Z=2, D c =1.378Mg/m 3, μ =0.233mm -1, F(000)=520, λ (Mo Kα )=0.71073, T =293(2)K, final R =0.071 and wR =0.1953 for 4177 independent reflections with I≥2σ(I ). In the title compound, each molecule is linked to five neighbour molecules by hydrogen bonds forming layers perpendicular to the a axis and molecules in each layer are linked by a two dimensional network of hydrogen bonds in the bc plane.

关键词： crystal structure synthesis diethyl (4 6 diamino 1 3 5 triazine 2 yl) phosphonate layer

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太平洋褶柔鱼内脏团营养成分分析与评价

食品安全质量检测学报 2024年第11期15卷 294-300页

作者：辛云刘小芳王西西李爽姜维龙莉军蒋永毅冷凯良中国水产科学研究院黄海水产研究所农业农村部极地渔业可持续利用重点实验室青岛266071 浙江海洋大学国家海洋设施养殖工程技术研究中心舟山316022 威海博宇食品有限公司威海264205 青岛菲优特检测有限公司青岛266112

目的研究太平洋褶柔鱼(Todarodes pacificus)内脏团的营养成分和价值。方法通过系统测定太平洋褶柔鱼内脏团的基本营养成分组成、脂肪酸组成、氨基酸组成、矿物质元素组成和脂溶性维生素含量,对其营养品质进行分析评价。结果太平洋褶柔... 详细信息

目的研究太平洋褶柔鱼(Todarodes pacificus)内脏团的营养成分和价值。方法通过系统测定太平洋褶柔鱼内脏团的基本营养成分组成、脂肪酸组成、氨基酸组成、矿物质元素组成和脂溶性维生素含量,对其营养品质进行分析评价。结果太平洋褶柔鱼内脏团的水分含量为(64.19±0.78)%,蛋白质、粗脂肪、碳水化合物和灰分的含量分别占干基的(49.68±1.89)%、(32.40±0.14)%、(10.31±1.94)%和(5.70±0.14)%;总脂质中甘油酯、磷脂和游离脂肪酸的含量分别为(55.42±2.65)%、(13.33±1.31)%和(22.64±0.70)%;总脂质中的多不饱和脂肪酸含量丰富,且主要为二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA),二者分别占总脂肪酸含量的(11.56±0.04)%和(27.86±0.09)%;氨基酸组成符合联合国粮农组织/世界卫生组织规定的优质蛋白标准;矿物质元素含量丰富,Zn和Fe等微量元素的含量较高;维生素D和维生素E等脂溶性维生素的含量较为丰富。结论太平洋褶柔鱼内脏团是优质蛋白、Omega-3系列多不饱和脂肪酸、脂溶性维生素等营养成分的良好来源,值得进一步开发利用。

关键词：太平洋褶柔鱼内脏团营养评价氨基酸组成脂肪酸组成脂溶性维生素

Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MS^n and HPLC-UV/ELSD

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 604-614页

作者： Shu-fang WANG Jing leng Yi-min XU Mei-ling FENG Institute of Pharmaceutical Informatics Zhejiang University School of Pharmaceutical Sciences Zhejiang University of Technology

Xiongdankaiming tablet (XDKMT), a well-known compound in traditional Chinese medicine, is widely used for the treatment of acute iridocyclitis and primary open-angle glaucoma. In this paper, accurate and reliable methods were developed for the identification of 20 constituents using high-performance liquid chromatography with photodiode array and electron spray ionization-mass spectrometry (HPLC-PDA/ESI-MSn ), and determination of nine of the constituents (chlorogenic acid, gentiopicroside, isochlorogenic acid B, diosmetin-7-O-β-D-glucopyranoside, apigenin, diosmetin, tauroursodeoxycholic acid, acacetin, and taurochenodeoxycholic acid) was developed using HPLC with ultraviolet absorption detector and evaporative light scattering detector (HPLC-UV/ELSD) for the first time. The best results were obtained on a Zorbax SB-C18 column with gradient elution using water (0.1% formic acid) (A) and methanol (0.1% formic acid) (B) at a flow rate of 0.7 ml/min. Tauroursodeoxycholic acid and taurochenodeoxycholic acid, owing to their low UV absorption, were detected by ELSD. The other seven compounds were analyzed by HPLC-UV with variable wavelengths. The calibration curves of all nine constituents showed good linear regression (R2 >0.9996) within the linearity ranges. The limits of detection and quantification were in the ranges of 0.0460-9.90 μg/ml and 0.115-24.8 μg/ml, respectively. The accuracy, in terms of recovery, varied from 95.3% to 104.9% with relative standard deviations (RSDs) less than 4.4%. Precision (with the intra- and inter-day variations less than 4.4%) was also suitable for its intended use. The developed method was successfully applied for the analysis of major components in XDKMT, which provides an appropriate method for the quality control of XDKMT.

关键词： Traditional Chinese medicine (TCM) compound recipe Xiongdankaiming tablet HPLC-UV/ELSD HPLC-MS Quality control

Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice

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Zoological Research 2022年第1期43卷 64-74页

作者： Lin Zhang Shan-Shan Zhang Kai-fang Wang Yi-Hui Li Hui-Juan Xu Kuan-Xiang Sun Shi Ma Hong-Mei leng Si-Zhu Chen Wen-Jing Jia Xian-Jun Zhu Jie Li Department of Ophthalmology Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Sichuan Provincial Key Laboratory for Human Disease Gene Study and Department of Laboratory Medicine Sichuan Provincial People's HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical SciencesChengduSichuan 610072China Qinghai Key Laboratory of Qinghai-Tibetan Plateau Biological Resources Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningQinghai 810008China University of Chinese Academy of Science Beijing 100049China

Retinal angiogenesis is a critical process for normal retinal ***,uncontrolled angiogenesis can lead to pathological neovascularization(NV),which is closely related to most irreversible blindness-causing retinal *** the molecular basis behind pathological NV is important for the treatment of related ***-related protein 1(TWIST1)is a well-known transcription factor and principal inducer of epithelial-mesenchymal transition(EMT)in many human *** previous study showed that Twist1 expression is elevated in pathological retinal *** date,however,the role of TWIST1 in retinal pathological angiogenesis remains to be *** study the role of TWIST1 in pathological retinal NV and identify specific molecular targets for antagonizing pathological NV,we generated an inducible vascular endothelial cell(EC)-specific Twist1 transgenic mouse model(Tg-Twist1iEC+).Whole-mount retinas from Tg-Twist1iEC+mice showed retarded vascular progression and increased vascular density in the front end of the growing retinal vasculature,as well as aneurysm-like pathological retinal ***,overexpression of Twist1 in the ECs promoted cell proliferation but disturbed cell polarity,thus leading to uncontrolled retinal ***1 promoted pathological NV by activating the Wnt/β-catenin signaling pathway and inducing the expression of NV formation-related genes,thereby acting as a‘valve’in the regulation of pathological *** study identified the critical role of TWIST1 in retinal pathological NV,thus providing a potential therapeutic target for pathological NV.

关键词： Pathological angiogenesis TWIST1 Molecular markers Mouse model Retinal

COVID-19-associated monocytic encephalitis(CAME):histological and proteomic evidence from autopsy

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Signal Transduction and Targeted Therapy 2023年第2期8卷 705-715页

作者： Pei-Pei Zhang Zhi-Cheng He Xiao-Hong Yao Rui Tang Jie Ma Tao Luo Chuhong Zhu Tian-Ran Li Xindong Liu Dingyu Zhang Shuyang Zhang Yi-fang Ping Ling leng Xiu-Wu Bian Department of Pathology the First Affiliated Hospital of University of Science&Technology of China230036 HefeiAnhuiP.R.China Institute of Pathology&Southwest Cancer Center Southwest HospitalThird Military Medical University(Army Medical University)and Key Laboratory of Tumor ImmunopathologyMinistry of Education of China400038 ChongqingP.R.China Department of Pathology Ruijin HospitalSchool of MedicineShanghai Jiaotong University200025 ShanghaiP.R.China Stem Cell and Regenerative Medicine Lab Department of Medical Science Research CenterState Key Laboratory of Complex Severe and Rare DiseasesTranslational Medicine CenterPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical Sciences100730 BeijingP.R.China Department of Anatomy Key Laboratory for Biomechanics and Tissue Engineering of ChongqingState Key Laboratory of TraumaBurn and Combined injuryArmy Medical University(Third Military Medical University)400038 ChongqingP.R.China Wuhan Jinyintan Hospital(Wuhan Hospital for Infectious Diseases) 430015 WuhanHubeiP.R.China Department of Cardiology Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical Sciences100730 BeijingP.R.China

Severe neurological symptoms are associated with Coronavirus disease 2019(COVID-19).However,the morphologic features,pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic *** this study,neuropathological damages and infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining,ultrastructural examination under electron microscopy,and an image threshold method,in postmortem brains from nine critically ill COVID-19 patients and nine age-matched cadavers of healthy *** expressed proteins were identified by quantitative proteomic *** findings included neurophagocytosis,microglia nodules,satellite phenomena,extensive edema,focal hemorrhage,and infarction,as well as infiltrating mononuclear *** of COVID-19 brains revealed extensive activation of both microglia and astrocytes,severe damage of the blood-brain barrier(BBB)and various degrees of perivascular infiltration by predominantly CD14+/CD16+/CD141+/CCR7+/CD11c+monocytes and occasionally CD4+/CD8+T *** proteomic assays combined with bioinformatics analysis identified upregulated proteins predominantly involved in immune responses,autophagy and cellular metabolism in COVID-19 patient brains compared with control *** involved in brain development,neuroprotection,and extracellular matrix proteins of the basement membrane were downregulated,potentially caused by the activation of transforming growth factorβreceptor and vascular endothelial growth factor signaling ***,our results define histopathological and molecular profiles of COVID-19-associated monocytic encephalitis(CAME)and suggest potential therapeutic targets.

关键词： encephalitis metabolism histological

内镜下痔上直肠黏膜套扎术治疗内痔的临床疗效

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当代医学 2023年第29期29卷 96-98页

作者：叶长根朱晓佳冷芳胡娜戴华梅向阳方军杨力景德镇市第三人民医院消化内科江西景德镇333000

目的探究内镜下痔上直肠黏膜套扎术(SHB)治疗内痔的临床疗效。方法回顾性分析2019年11月至2021年3月于景德镇市第三人民医院行内镜下套扎治疗的88例Ⅰ~Ⅲ级伴出血和/或脱垂症状内痔患者的临床资料,根据套扎位置不同分为内镜下痔核套扎术... 详细信息

目的探究内镜下痔上直肠黏膜套扎术(SHB)治疗内痔的临床疗效。方法回顾性分析2019年11月至2021年3月于景德镇市第三人民医院行内镜下套扎治疗的88例Ⅰ~Ⅲ级伴出血和/或脱垂症状内痔患者的临床资料,根据套扎位置不同分为内镜下痔核套扎术(ERBL)组(n=47)和SHB组(n=41)。ERBL组行ERBL治疗,SHB组行SHB治疗,比较两组临床疗效、复发率及术后并发症发生率。结果两组治疗有效率和复发率比较差异无统计学意义。SHB组坠胀感、疼痛发生率均低于ERBL组,差异有统计学意义(P<0.05);两组出血、尿潴留发生率比较差异无统计学意义。结论内痔内镜下套扎术是一种可行、安全、有效的方法,SHB与ERBL疗效相当,但SHB术后并发症发生率更低,患者舒适度更高,更易接受。

关键词：内痔内镜下痔上直肠黏膜套扎术内镜下痔核套扎术复发率,并发症