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Silencing of MGMT with small interference RNA reversed resistance in human BCUN-resistant glioma cell lines

Chinese Medical journal 2011年第17期124卷 2605-2610页

作者： XIE Si-ming fang Mao GUO Hui ZHONG Xue-yun Department of Pathology Medical College Jinan University Guangzhou Guangdong 510632 China Department of Traditional Chinese Medicine Medical College Jinan University Guangzhou Guangdong 510632 China

Background Our previous study had cloned two glioma cell lines SWOZ1 and SWOZ2 isolated from parental glioma cell line SWO38. The 1,3-bis（2-chloroethyl）-l-nitrosourea （BCNU） resistance of SWOZ1 was higher than that of SWOZ2. Since Oe-methylguanine-DNA methyltransferase （MGMT） was thought to be closely related to BCNU resistance in glioma this study aimed to explore the function of MGMT in glioma resistant to BCNU. Methods A BCNU resistant glioma cell line SWOZ2-BCNU was established. The expression of MGMT was detected in SWOZ1, SWOZ2 and SWOZ2-BCNU. Small interferencing RNA targeting MGMT was used to silence the expression of MGMT in resistant cell lines SWOZ1 and SWOZ2-BCNU. The cytotoxicity of BCNU to these cells was measured using the cell counting kit-8 assay. Statistical analysis was carried out by one-way analysis of variance in statistical package SPSS 13.0. Results The resistance of SWOZl and SWOZ2-BCNU against BCNU was 4.9-fold and 5.3-fold higher than that of SWOZ2. The results of quantitative RT-PCR and Western blotting confirmed that MGMT was both significantly increased in SWOZ1 and SWOZ2-BCNU compared to SOWZ2. After transfection with small interferencing RNA targeting MGMT, a decreased level of MGMT mRNA expression in SWOZ1 and SWOZ2-BCNU for more than 75% compared to negative control was found and confirmed by Western blotting. As a result, the resistance against BCNU was reversed for about 50% both in the BCNU-resistant cell lines SWOZ1 and SWOZ2-BCNU. Conclusions Silencing MGMT with specific small interferencing RNA can reverse the BCNU resistant phenotype in these glioma cell lines. MGMT may play an important role both in intrinsic and acquired BCNU-resistance in glioma.

关键词： glioma MGMT," small interferencing RNA drug resistance 1,3-bis(2-chloroethyl)-l-nitrosourea

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河流洪水风险控制的合作博弈分析

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中国管理科学 2023年第9期31卷 45-51页

作者：单而芳上海大学管理学院上海200444

随着社会经济的发展和气候变暖等因素的影响,河流洪水风险控制面临诸多挑战。护土保水是防止洪水风险的重要措施之一。如何激励同一河流沿岸的利益主体,使其自愿改变土地利用方式,以达到护土保水的目的,各利益主体之间的利益分配是非常... 详细信息

随着社会经济的发展和气候变暖等因素的影响,河流洪水风险控制面临诸多挑战。护土保水是防止洪水风险的重要措施之一。如何激励同一河流沿岸的利益主体,使其自愿改变土地利用方式,以达到护土保水的目的,各利益主体之间的利益分配是非常关键的问题之一。本文以合作博弈理论作为工具,针对同一河流沿岸利益主体的总利益,提出了基于Shapley值的分配方案,由此建立了上、下游利益主体之间的合理补偿机制,以此激励河流上、下游利益主体之间的合作,使上游利益主体注重森林作为天然保水特征所发挥的作用,增强上游的蓄水能力,从而达到洪水风险控制的目标。

关键词：合作博弈洪水风险 Shapley值补偿有向图

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Full-scale model tests and nonlinear analysis of prestressed concrete simply supported box girders

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Engineering Sciences 2014年第1期12卷 67-76页

作者： fang Zhi Tang Shenghua He Xin College of Civil Engineering Hunan University

Full-scale model tests were carried out on a 30 m span prestressed concrete box girder and a 20 m span prestressed concrete hollow slab. Failure models were prestressed reinforcement tensile failure and crashing of roof concrete, respectively. The ductility indexes of the box girder and hollow slab were 1.99 and 1.23, respectively, according to the energy viewpoint. Based on the horizontal section hypothesis, the nonlinear computation procedure was established using the limited banding law, and it could carry out the entire performance analysis including the unloading, mainly focusing on the ways to achieve the unloading curves computation through stress-strain, moment-curvature and load-displacement curves. Through the procedure, parameters that influence on the bearing capacity, deformation performance and ductility of the structures were analyzed. Those parameters were quantity of prestressed reinforcement and tension coefficients of prestressed reinforcement. From the analysis, some useful conclusions can be obtained.

关键词： prestressed concrete box girder full-scale model test nonlinear analysis bearing capacity ductility

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Effect of Polymerization Temperature on Cationic Emulsion Properties

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科技视界 2013年第30期 197-198页

作者：贺东琴 fang Kuan-jun Laboratory of Fiber Materials and Modern Textiles The Growing Base for State Key LaboratoryCollege of Chemical and Environmental Engineering Qingdao University

The objective of this work was to analyze the effects of the polymerization temperature on the kinetic features(instantaneous and overall conversions)and colloidal characteristics[mean particle diameter,stabilities]in the semicontinuous seeded cationic emulsion polymerization of styrene and butyl *** is obutyl amidine hydrochloride was used as an *** surfactants wascetyl trimethyl ammonium *** results showed that the final instant when the polymerization temperature of 85℃emulsion highest conversion rate,and the temperature of the emulsion particle size,centrifugal stability is the best,85℃emulsion in the emulsion anti-dilution and acid salt stability also showed excellent stability.

关键词：英语教学教学方法阅读教学课外阅读

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工程建设安全生产管理的相关主体行为关系模型分析与政策改进

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土木工程学报 2014年第9期47卷 128-134页

作者：周建亮方东平房继寒中国矿业大学江苏徐州221116 清华大学北京100084

在现行工程建设安全生产管理制度中,业主、监理和施工单位是与工程现场联系最为密切的主体,但三者之间在安全生产管理上的分工和责任划分,一直存在较大的争议。通过结构方程模型方法,基于当前的政策和制度要求下识别业主、监理单位和施... 详细信息

在现行工程建设安全生产管理制度中,业主、监理和施工单位是与工程现场联系最为密切的主体,但三者之间在安全生产管理上的分工和责任划分,一直存在较大的争议。通过结构方程模型方法,基于当前的政策和制度要求下识别业主、监理单位和施工单位与安全生产管理相关的行为集合,构建三方主体履行安全生产管理职责的行为关系模型,讨论三者在安全生产管理上的复杂角色与行为关系模型之间的内在联系,提出提升业主安全责任要求、细化施工单位安全责任和调整事故安全责任主体划分思路的建议。

关键词：业主监理单位施工单位建设安全生产行为关系模型

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伤残调整生命年衡量尘肺患者疾病负担的初步研究

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中华劳动卫生职业病杂志 2005年第2期23卷 159-160页

作者：房巧玲山东省济南市职业病防治院职业病科 250013

目的　探讨尘肺病预防的经济效益和社会效益。方法　调查住院尘肺患者的经济损失和伤残状况,使用伤残调整生命年(DALY)全面衡量患者的尘肺疾病负担,分析影响伤残调整生命年的有关因素,定量说明预防尘肺发生可避免的经济损失和健康寿命... 详细信息

目的　探讨尘肺病预防的经济效益和社会效益。方法　调查住院尘肺患者的经济损失和伤残状况,使用伤残调整生命年(DALY)全面衡量患者的尘肺疾病负担,分析影响伤残调整生命年的有关因素,定量说明预防尘肺发生可避免的经济损失和健康寿命损失。结果　DALY的主要影响因素为年龄、尘肺分期、伤残等级、尘肺并发结核4项因素。136人DALY损失平均为8.37个健康年,年平均直接经济损失为16 595元,年平均总经济损失为56 855元。结论　预防尘肺发病是减少其疾病负担的关键。

关键词：尘肺患病代价寿命表预防经济效益社会效益

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Research on development of surface-roughness variation and control technology of the automotive plates and rolls

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Baosteel Technical Research 2019年第4期13卷 8-19页

作者： QU Haixia GU Tingquan fang Baiyou Research Institute Baoshan Iron&Steel Co.Ltd.Shanghai 201999China Baosteel-NSC Automotive Steel Sheets Co. Ltd.Shanghai 201900China

Surface roughness considerably influences the stamping and coating performance of the automotive plates;however,the surface roughness of the automotive plates is transferred from the roll during the rolling ***,the variation and control technology regarding the roughness of the automotive plates and rolls has drawn increasing attention from researchers in China and around the *** this study,the research methods,analytical techniques,prediction models,transfer rules,and control technologies regarding the surface roughness of the automotive plates and rolls were *** these,an online measurement system for automotive-plate and roll surface roughness has been applied to the production lines of Germany,China,Belgium,Holland,Austria,and other countries and has achieved remarkable *** measurement and timely feedback regarding the automotive-plate and roll surface roughness are needed to ensure favorable conditions for the establishment of automotive-plate roughness-control measurement.

关键词： automotive plate roll surface roughness transferring rate control technology

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CFRP片材约束混凝土短柱徐变性能及极限承载力试验

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中国铁道科学 2007年第2期28卷 55-60页

作者：曹国辉方志饶欣频方智锋湖南大学土木工程学院湖南长沙410082 湖南城市学院土木工程学院湖南益阳413000

通过不同形式轴压短柱试件727d徐变及极限承载力试验，研究长期持续荷载下徐变对CFRP约束混凝土柱极限承载力的影响。研究结果表明：CFRP约束混凝土柱的徐变发展趋势与素混凝土柱及钢筋混凝土柱基本相同；其徐变系数明显小于素混凝土柱... 详细信息

通过不同形式轴压短柱试件727d徐变及极限承载力试验，研究长期持续荷载下徐变对CFRP约束混凝土柱极限承载力的影响。研究结果表明：CFRP约束混凝土柱的徐变发展趋势与素混凝土柱及钢筋混凝土柱基本相同；其徐变系数明显小于素混凝土柱，且混凝土强度等级愈低，徐变系数减小愈显著，而轴压比对徐变系数的影响较小，CFRP约束圆柱体的极限应变较CFRP约束棱柱体的极限应变大；长期荷载作用引起CFRP约束柱轴心受压极限变形降低4．5％～4．8％，引起无约束柱轴心受压柱极限变形降低23．2％～35．2％；CFRP能显著提高约束混凝土的极限承载力，且CFRP约束钢筋混凝土柱承载力的提高较CFRP约束素混凝土柱要小。

关键词： CFRP 约束混凝土柱徐变性能极限承载力加固

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Barley FASCIATED EAR genes determine inflorescence meristem size and yield traits

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The Crop journal 2023年第3期11卷 679-691页

作者： Chengyu Wang Xiujunan Yang Yueya Zhang Chaoqun Shen jin Shi Chongjing Xia Taohong fang Qiang Tu Ling Li Xinli Zhou Dabing Zhang Gang Li School of Life Sciences and Engineering Southwest University of Science and TechnologyMianyang 629000SichuanChina School of Agriculture Food and WineWaite Research InstituteThe University of AdelaideAdelaide 5005SAAustralia School of Life Science and Biotechnology Shanghai Jiao Tong UniversityShanghai 200240China Department of Plant Pathology College of Plant ProtectionNanjing Agricultural UniversityNanjing 210095JiangsuChina

In flowering plants,the inflorescence meristem(IM)provides founder cells to form successive floral meristems,which are precursors of fruits and *** activity and developmental progression of IM are thus critical for yield production in seed *** some cereals,such as rice(Oryza sativa)and maize(Zea mays),the size of undifferentiated IM,which is located at the inflorescence apex,is positively associated with yield traits such as spikelet ***,the relationship between IM size and yieldrelated spike traits remains unknown in the Triticeae *** we report that IM size has a negative correlation with yield traits in barley(Hordeum vulgare).Three FASCIATED EAR(FEA)orthologs,HvFEA2,HvFEA3,and HvFEA4,regulate IM size and spike morphogenesis and ultimately affect yield *** HvFEAs genes are highly expressed in developing spikes,and all three loss-of-function mutants exhibit enlarged IM size,shortened spikes,and reduced spikelet number,which may lead to reduced grain *** variations identified in HvFEAs indicate selection events during barley *** further reveal that HvFEA4,as a transcription factor,potentially targets multiple pathways during reproductive development,including transcriptional control,phytohormone signaling,and redox *** roles of barley FEA genes in limiting IM size and promoting spikelet formation suggest the potential of increasing yield by manipulating IM activity.

关键词： Inflorescence meristem Yield traits FASCIATED EAR genes Gene expression Barley

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Combination of renoportal anastomosis and inferior mesenteric vein-portal anastomosis in liver transplantation:A new portal reconstruction technique

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 182-185页

作者： Guo-Ling Lin Min Xiao Li Zhuang Yu Yang Qi-Yong Li jian-fang Lu Meng-Xia Li Shu-Sen Zheng Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryShulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhouChina Division of Hepatobiliary and Pancreatic Surgery Department of NursingShulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhouChina Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryZhejiang University School of MedicineHangzhouChina Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryThe First Affiliated HospitalZhejiang University School of MedicineHangzhouChina

Liver transplantation (LT) is the only way to cure end-stage liver disease with or without tumors in the last few decades [1].However,critical issues such as how to rebuild portal flow in patients with portal vein thrombosis (PVT) or superior mesenteric vein (SMV) thrombosis have been challenging for *** portal flow is critical in LT because more than 75%of the liver’s blood supply comes from the portal vein and the rest comes from the hepatic *** is a universal problem in LT.

关键词： anastomosis portal Liver

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