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cDNA of an arylphorin-type storage protein from Pieris rapae with parasitism inducible expression by the endoparasitoid wasp Pteromalus puparum

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Insect Science 2009年第3期16卷 227-236页

作者： Jia-Ying Zhu Gong-Yin Ye Qi fang cui Hu Zunnu-Raen Akhtar State Key Laboratory of Rice Biology Institute of Insect Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou China

This report presents the cDNA cloning of a storage protein, PraAry, from Pieris rapae and investigates its expression regulated by parasitism of an endoparasitoid wasp Pteromalus puparum. The full-length eDNA of PraAry is 2 270 nucleotides and contains a 2 121 nucleotide open reading frame encoding 707 amino acids with calculated molecular weights of approximately 83 kDa. Analysis of the primary protein sequence revealed that it possesses a signal peptide of 16 amino acids at the N-terminus and contains two highly conserved storage protein signature motifs. According to both phylogenetic analysis and the criteria for amino acid composition, PraAry belongs to the subfamily of arylphorin-type storage protein （1.42% methionine and 18.82% aromatic amino acids）. Reverse transcription- polymerase chain reaction analysis indicated that the transcriptional level of PraAry mRNA in P. rapae pupae fat body is inducible in response to parasitism by P. puparum.

关键词： cloning gene expression parasitism Pieris rapae Pteromalus puparum storage protein

Synthesis and characterization of luminescent TiO2/polymer nanocomposites

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Synthesis and characterization of luminescent TiO2/polymer n...

Activity regulation and applications of metal-organic framework-based nanozymes

第八届中国功能材料及其应用学术会议

作者： cui fang YAO Tong-jie YU Jing SUN Ke-ning The Academy of Fundamental and Interdisciplinary Science Harbin Institute of Technology

ISBN: (纸本)9783037859698

Transparent luminescent TiO/polymer nanocomposites were obtained through the combined use of copolymerization of a novel titanium ioncontainingmonomer with a liquid-solid *** titanium ions are covalently introduced intopolymer chains and transformed into TiO nanocrystals directly in the solid-state polymer *** allows the generationof high-quality anatase TiO nanocrystals with a narrow size distribution in the polymer *** luminescence of the TiO nanocrystals is dominated by band-edge luminescence at room temperature.

关键词： TiO2 nanocomposites luminescent nanocrystals

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Rare Metals 2024年第3期43卷 900-914页

作者： Ge fang Shou-Xin Bao Gen-Xiu Zhou cui-cui Ge State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSchool for Radiological and Interdisciplinary Sciences(RAD-X)Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsSoochow UniversitySuzhou 215123China Suzhou Nanomicro Technology Co. LtdSuzhou 215000China

As a new generation of artificial enzymes,nanozymes show outstanding advantages such as high stability,low cost,and facile synthesis,which endow them with promising applications in biomedical and environmental *** the various reported nanozymes,metal-organic frameworks(MOFs)could mimic the active center of natural enzymes and provide a hydrophobic environment,which makes MOFs attractive alternatives to natural *** to the highly structural diversity and tailorability of MOFs,rational design will contribute to improve the activity of MOF-based nanozymes and promote their potential applications in both biomedical and environmental ***,a comprehensiye suminary of activity regulatory strategies of MOF-based nanozymes is urgently ***,we summarized the activity regulatory strategies of MOFs with intrinsic enzyme-like activities via modulation of metal nodes,ligands,structures and *** the applications of MOF-based nanozymes in biosensing,hazardous degradation,antibacterial,and cancer therapy were also ***,the current challenges and future perspectives were discussed in *** is highly expected that this review will provide a better understanding on the rational design of novel high-performance MOF-based nanozymes.

关键词： Metal-organic frameworks(MOF)-based nanozymes Activity regulation Biosensing Cancer therapy Hazardous degradation Antibacterial

To Blame or Not?Modulating Third-Party Punishment with the Framing Effect

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Neuroscience Bulletin 2022年第5期38卷 533-547页

作者： Jiamiao Yang Ruolei Gu Jie Liu Kexin Deng Xiaoxuan Huang Yue-Jia Luo fang cui School of Psychology Shenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Affective and Social Neuroscience Magnetic Resonance Imaging CenterCenter for Brain Disorders and Cognitive SciencesShenzhen UniversityShenzhen 518060China CAS Key Laboratory of Behavioral Science Institute of PsychologyChinese Academy of SciencesBeijing 100101China Department of Psychology University of Chinese Academy of SciencesBeijing 100049China Beijing Key Laboratory of Applied Experimental Psychology Faculty of PsychologyBeijing Normal UniversityBeijing 100875China

People as third-party observers,without direct self-interest,may punish norm violators to maintain social ***,third-party judgment and the follow-up punishment might be susceptible to the way we frame(i.e.,verbally describe)a norm *** conducted a behavioral and a neuroimaging experiment to investigate the above phenomenon,which we call the“third-party framing effect”.In these experiments,participants observed an anonymous perpetrator deciding whether to keep her/his economic benefit while exposing a victim to a risk of physical pain(described as“harming others”in one condition and“not helping others”in the other condition),then they had a chance to punish that perpetrator at their own *** results showed that the participants were more willing to execute third-party punishment under the harm frame compared to the help frame,manifesting a framing ***-reported anger toward perpetrators mediated the relationship between empathy toward victims and the framing ***,activation of the insula mediated the relationship between mid-cingulate cortex activation and the framing effect;the functional connectivity between these regions significantly predicted the size of the framing *** findings shed light on the psychological and neural mechanisms of the third-party framing effect.

关键词： Framing effect Third-party punishment Functional magnetic resonance imaging Mid-cingulate cortex Insula

石榴籽油的提取、营养组成及健康功效研究进展

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中国油料作物学报 2024年第1期46卷 208-220页

作者：胡子聪胡超凡李彦坡蒋巧俊李群和房翠兰温州市农业科学研究院食品科学研究所浙江温州325006 温州科技职业学院农业与生物技术学院浙江温州325006 温州市特色食品资源工程技术研究中心浙江温州325006 重庆市九龙坡区疾病预防控制中心重庆400039

石榴籽油是从石榴加工剩余物石榴籽中提取得到的一种功能性植物油,它具有抗氧化、抗炎症、抗肿瘤和预防心血管疾病等功效。本文从石榴籽油的提取方法、营养组成和健康功效等方面,对近年来国内外有关石榴籽油的研究结果进行了全面综述。... 详细信息

石榴籽油是从石榴加工剩余物石榴籽中提取得到的一种功能性植物油,它具有抗氧化、抗炎症、抗肿瘤和预防心血管疾病等功效。本文从石榴籽油的提取方法、营养组成和健康功效等方面,对近年来国内外有关石榴籽油的研究结果进行了全面综述。水酶法、溶剂萃取法、超临界流体萃取法和物理场辅助提取法对石榴籽油的得率和脂肪酸组成有明显的影响。尽管源于不同产地和不同提取方法的石榴籽油在脂肪酸组成上存在差异,但整体上来看,石榴籽油中不饱和脂肪酸占比达到88.57%~95.33%,其中以石榴酸为主(60.62%~81.40%),它是石榴籽油能够发挥一系列营养功效的主要活性成分。因此,石榴籽油是一种具有高营养价值和潜在利用价值的木本植物油,本文旨在为石榴籽油的进一步研究及其在食品、生物医药等领域的开发利用提供理论参考。

关键词：石榴籽油提取方法营养组成石榴酸健康功效活性机制

时效性激励护理对脑梗塞患者负性情绪、创伤后成长和神经功能恢复的影响

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川北医学院学报 2024年第2期39卷 280-284页

作者：师俊明张燕李翠芳秦岚首都医科大学附属北京友谊医院急诊科北京101100

目的:探讨时效性激励护理对脑梗塞患者负性情绪、创伤后成长和神经功能恢复的影响。方法:选取116例脑梗塞患者为研究对象,其中实施常规护理的58例设为对照组,实施时效性激励护理的58例设为观察组。两组均干预至患者出院,比较两组干预前... 详细信息

目的:探讨时效性激励护理对脑梗塞患者负性情绪、创伤后成长和神经功能恢复的影响。方法:选取116例脑梗塞患者为研究对象,其中实施常规护理的58例设为对照组,实施时效性激励护理的58例设为观察组。两组均干预至患者出院,比较两组干预前后负性情绪[焦虑自评量标(SAS)和抑郁自评量表(SDS)]、创伤后成长[创伤后成长量表(PTGI)]、治疗依从性、神经功能情况[美国国立卫生院神经功能缺损评分表(NIHSS)]及主观幸福感[总体幸福感量表(GWB)]。结果:干预后,两组SAS及SDS评分均下降(P<0.05),且观察组低于对照组(P<0.05);两组PTGI量表总分及各维度评分均上升(P<0.05),且观察组高于对照组(P<0.05)。观察组治疗依从性优良率为94.83%,高于对照组的82.76%(P<0.05)。干预后,两组NIHSS评分均下降(P<0.05),且观察组低于对照组(P<0.05);两组GWB量表总分及各维度评分均上升(P<0.05),且观察组高于对照组(P<0.05)。结论:时效性激励护理可改善脑梗塞患者负性情绪,提高其创伤后成长及治疗依从性,进而有效改善脑梗塞患者神经功能缺损症状,提升其主观幸福感。

关键词：脑梗塞时效性激励护理负性情绪创伤后成长神经功能

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肝硬化失代偿期合并肺结核的个体化治疗分析与药学监护

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中南药学 2024年第7期22卷 1939-1943页

作者：邓珍珍吴翠芳王胜峰中南大学湘雅三医院药学部长沙410013

1例硬化失代偿期患者持续高热,经验性抗感染治疗效果不佳,临床药师参与制订个体化抗结核+抗感染方案:异烟肼0.3 g qd+利福平0.45 g qd+乙胺丁醇0.75 g qd+左氧氟沙星0.6 g qd,抗结核治疗一周后患者体温恢复正常;治疗两周后,患者胸腔积... 详细信息

1例硬化失代偿期患者持续高热,经验性抗感染治疗效果不佳,临床药师参与制订个体化抗结核+抗感染方案:异烟肼0.3 g qd+利福平0.45 g qd+乙胺丁醇0.75 g qd+左氧氟沙星0.6 g qd,抗结核治疗一周后患者体温恢复正常;治疗两周后,患者胸腔积液较前明显减少,但复查总胆红素升高(61μmol·L^(-1)),考虑患者出现了抗结核药物性肝损伤。临床药师评估病情与肝功能情况,决定继续抗结核治疗,并加用腺苷蛋氨酸保肝治疗,一周后复查胆红素较前未再升高;两周后肝功能显著好转,顺利出院。肝硬化患者的抗结核治疗方案制订需结合患者的病情和抗结核药物的特点进行个体化选择,以寻求获益与风险的平衡。

关键词：肝硬化失代偿期肺结核个体化药物性肝损伤药学监护

Flame Morphology and Characteristic of Co-Firing Ammonia with Pulverized Coal on a Flat Flame Burner

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Journal of Thermal Science 2024年第5期33卷 1935-1945页

作者： WANG Shengye cui Mingshuang LIU Pengzhong DI Yi NIU fang China Coal Research Institute Beijing 100013China Beijing Tiandi Rongchuang Technology Co.Ltd. Beijing 100013China National Energy Technology&Equipment Laboratory of Coal Utilization and Emission Control Beijing 100013China

Ammonia as a new green carbon free fuel co-combustion with coal can effectively reduce CO_(2)emission,but the research of flame morphology and characteristics of ammonia-coal co-combustion are not *** this work,we studied the co-combustion flame of NH_(3)and pulverized coal on flat flame burner under different oxygen mole fraction(X_(i,O_(2)))and NH_(3)co-firing energy ratios(E_(NH_(3))).We initially observed that the introduction of ammonia resulted in stratification within the ammonia-coal co-combustion flame,featuring a transparent flame at the root identified as the ammonia combustion *** to challenges in visually observing the ignition of coal particles in the ammonia-coal co-combustion flame,we utilized Matlab software to analyze flame images across varying E_(NH_(3))and X_(i,O_(2)).The analysis indicates that,compared to pure coal combustion,the addition of ammonia advances the ignition delay time by 4.21 ms to 5.94 *** E_(NH_(3))increases,the ignition delay time initially decreases and then ***,an increase in X_(i,O_(2))results in an earlier ignition delay *** burn-off time and the flame divergence angle of pulverized coal demonstrated linear decreases and increases,respectively,with the growing ammonia *** addition of ammonia facilitates the release of volatile matter from coal ***,in high-ammonia environments,oxygen consumption also impedes the surface reaction of coal ***,measurements of gas composition in the ammonia-coal flame flow field unveiled that the generated water-rich atmosphere intensified coal particle gasification,resulting in an elevated concentration of ***,nitrogen-containing substances and coke produced during coal particle gasification underwent reduction reactions with NO_(x),leading to reduced NO_(x)emissions.

关键词： co-firing ammonia with coal flame characteristic gas component ignition delay time burn-off time flame divergence angle flat flame burner flame morphology

Resonance Light Scattering Method for Determination of Amikacin with Potassium Ferrioxalate as a Probe

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Chemical Research in Chinese Universities 2010年第3期26卷 366-370页

作者： HU Xiao-li AN Lan-xiang LIU Shao-pu LIU Zhong-fang LI cui-xia Key Laboratory of Luminescence and Real-Time Analysis Ministry of Education School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 P. R. China

In a weak acid medium, potassium ferrioxalate（PF） can react with some aminoglycoside（AGs） antibiotics, such as amikacin（AMK）, kanamycin（KANA）, tobramycin（TOB） and gentamicin（GEN）, to form ion-association complexes. It results in the enhancement of resonance light scattering（RLS） in different degrees. The maximum scattering peaks are all located at 345 nm. Among them, the relative scattering intensity（AIRLs） of AMK system is much higher than that of KANA, TOB or GEN. Therefore the method is more propitious to the determination of trace amounts of AMK. The optimum reaction conditions, influencing factors, and the relationship between scattering intensity and concentration of antibiotics were investigated by means of the proposed method. The enhancement of RLS signals is directly proportional to the concentration of antibiotics in a certain range of concentration. A new resonance light scattering method for the determination of AMK and other aminoglycoside antibiotics with [Fe（C2O4）3]^3- as a probe is thus established based on it. The method exhibits high sensitivity and good selectivity. The detection limit（3σ） for AMK is 1.8 ng/mL. The method can be applied to the determination of AMK in clinical serum samples. The reaction mechanism and the reasons for RLS enhancement are discussed in this paper.

关键词： Resonance light scattering Amikacin Aminoglycoside antibiotics Potassium ferrioxalate