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敲除LTA4H基因的PK-15细胞系构建及其对口蹄疫病毒复制的影响

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微生物学报 2024年第3期64卷 733-744页

作者：王家丽蒲秀瑛杨帆邵文华黄梦瑶曹伟军郑海学张伟兰州理工大学生命科学与工程学院甘肃兰州730050 中国农业科学院兰州兽医研究所/兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室甘肃兰州730000 甘肃省病原生物学基础学科研究中心甘肃兰州730046

【目的】利用CRISPR/Cas9基因编辑技术构建白三烯A4水解酶(leukotriene A4 hydrolase,LTA4H)基因缺失的猪肾细胞系(porcine kidney-15,PK-15),探究LTA4H对口蹄疫病毒(foot-and-mouth disease virus,FMDV)复制的影响,为开展LTA4H功能研究及调控病毒复制机制研究提供理论依据。【方法】设计2条针对猪LTA4H基因的引导RNA(small guide RNA,sgRNA),分别构建至载体pX459-puro-MCS中;将CRISPR重组质粒转染PK-15细胞,用嘌呤霉素(puromycin)抗生素筛选,并通过有限稀释法筛选单克隆细胞,之后通过Western blotting和测序检测LTA4H基因的敲除,获得LTA4H基因功能缺失细胞系。使用Western blotting、RT-qPCR及病毒滴度测定等方法检测敲除LTA4H基因后对FMDV复制及相关蛋白的表达情况。【结果】获得的单克隆敲除细胞系与野生型细胞相比,能够显著抑制FMDV复制。【结论】本研究成功构建了LTA4H基因敲除的PK-15细胞系,证明LTA4H对FMDV的复制具有促进作用,研究结果为后续LTA4H功能研究提供理论依据。

关键词： CRISPR/Cas9 白三烯A4水解酶细胞系口蹄疫病毒

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喉上神经阻滞联合气管内表面麻醉在老年高血压患者气管插管中的应用分析

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老年医学与保健 2024年第4期30卷 1054-1059页

作者：蒲玉碧杨帆卢海刘虹遂宁市中心医院麻醉科四川遂宁629000

目的探究喉上神经阻滞联合气管内表面麻醉在老年高血压患者全身麻醉气管插管中应用效果,进一步明确老年高血压其麻醉方案优化的必要性,以期为患者临床麻醉提供新的方法,提高手术治疗效果。方法选取2021年3月—2023年3月于遂宁市中心医... 详细信息

目的探究喉上神经阻滞联合气管内表面麻醉在老年高血压患者全身麻醉气管插管中应用效果,进一步明确老年高血压其麻醉方案优化的必要性,以期为患者临床麻醉提供新的方法,提高手术治疗效果。方法选取2021年3月—2023年3月于遂宁市中心医院就诊的172例老年高血压患者的病例资料作为观察对象,采用简单随机化法将患者分为观察组与对照组,每组86例。对照组采用全麻诱导插管,观察组采用喉上神经阻滞联合气管内表面麻醉。比较2组插管情况(一次性气管插管成功率、总插管成功率、平均插管时间、呛咳发生率),对比2组插管前、插管时、插管后(T1、T2、T3)的血流动力学[心率(HR)、血压(SBP、DBP)及血氧饱和度(SpO_(2))]指标值的变化,T1、T3时2组血清[肾上腺素(E)、去甲肾上腺素(NE)及皮质醇(Cor)]水平,记录2组插管期间不良事件的发生情况。手术结束后对患者的苏醒情况进行记录。结果观察组一次性插管成功率(97.67%)高于对照组(89.53%),平均插管时间[(34.52±5.19)s]低于对照组[(40.76±5.49)s](均P0.05)。T2、T3时观察组HR、SBP、DBP值低于对照组,SpO_(2)值高于对照组(均P<0.05)。T3时观察组E、NE水平[(35.58±6.26)kg/m^(2)、(158.76±14.36)g/L]低于对照组[(47.30±9.22)kg/m^(2)、(167.74±14.46)g/L](均P<0.05)。观察组肌肉松弛恢复时间、PAED评分、拔管时间[(8.16±2.35)min、(8.67±1.32)分、(10.56±3.30)min]均少/低于对照组[(9.79±3.05)min、(10.40±1.38)分、(12.67±3.20)min](均P<0.05)。结论喉上神经阻滞联合气管内表面麻醉降低老年高血压患者插管反应的效果显著,有利于维持患者血流动力学稳定,具有一定临床应用价值。

关键词：老年高血压喉上神经阻滞气管内表面麻醉插管成功率血流动力学

Meta- analysis of association between K469E polymorphism of the ICAM-1 gene and retinopathy in type 2 diabetes

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 603-607页

作者： Wen-Ying fan Ning-pu Liu Beijing Tongren Eye Center Beijing Tongren HospitalBeijing Ophthalmology and Visual Sciences Key LaboratoryCapital Medical University

AIM: To collectively evaluate the association of intercellular adhesion molecule-1(ICAM-1) gene K469 E polymorphism(rs5498) with diabetic retinopathy(DR) in patients with type 2 diabetic mellitus(T2DM). METHODS: Overall review of available literatures relating K469 E polymorphism to the risk of DR was conducted on 4 electronic databases. Meta-analysis was performed by Stata 12.0 to calculate pooled odds ratios(ORs). Potential sources of heterogeneity and bias were ***: Seven studies with genotype frequency data including 1120 cases with DR and 956 diabetic controls free of DR were included. Meta-analysis did not show significant association of K469 E polymorphism with DR(P >0.05). A statistically significant association was detected between the K469 E polymorphism and proliferative DR(PDR) in Asians only in dominant model(GG+AG vs AA) with pooled OR of 0.729(95%CI: 0.564-0.942, P=0.016, P heterogeneity=0.143), however, this association was not detected in recessive model(AG +AA vs GG;OR=1.178, 95%CI: 0.898-1.545, P =0.236, P heterogeneity=0.248)or allelic model(G vs A; OR=0.769, 95% CI: 0.576-1.026,P =0.074, P heterogeneity=0.094). No publication bias was found by Funnel plot, Begg’s and Egger’s test. CONCLUSION: This research found no statistically significant association between ICAM-1 gene K469 E polymorphism and DR in patients with T2 DM, but showed significant association of the K469 E polymorphism with PDR in Asian diabetic patients only in dominant model. Further investigation would be required to consolidate the conclusion.

关键词： K469E polymorphism rs5498 intercellular adhesion molecule-1 diabetic retinopathy type 2 diabetes Meta-analysis

The effect of horizontal Coriolis force on internal wave-mean flow interaction

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Acta Oceanologica Sinica 1988年第1期 27-42页

作者： fan zhisong

-In this paper, an improvement and revision of the theory of Miiller （1974, 1976）, has been made under two conditions: （1） the horizontal component of the Coriolis force has been taken into account in the equations of motion for the internal wave field; and （2） the role of internal waves with frequencies close to the inertial frequency has been considered. The values of the viscosity coefficients and the diffusivity coefficients obtained in this paper are: Theappearance of the cross-diffusion （vc） of momentum is a natural result from the effect of the horizontal Coriolis force. Therefore the role of the horizontal shear of the mean flow cannot be absolutely separated from that of the vertical shear. So far in testing Muller’s theory, the approximation of effective wave stress obtained by Ruddick and Joyce （1979） was extensively used, but it has to be revised under the conditions of this paper. The revised approximation shows that the internal waves with low frequencies close to the inertial frequency pl

关键词： wave flow

全光纤结构拉曼振荡器实现近1.8 kW激光输出

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红外与激光工程 2024年第5期53卷 268-270页

作者：范晨晨付敏郝修路黄善旻李阳陈子伦冷进勇姚天甫周朴国防科技大学前沿交叉学科学院湖南长沙410073 国防科技大学南湖之光实验室湖南长沙410073 国防科技大学高能激光技术湖南省重点实验室湖南长沙410073

拉曼光纤激光器在实现高功率特殊波长激光方面具有独特优势,受到了广泛关注和研究。近年来,随着渐变折射率多模光纤在拉曼激光器中的运用,不仅提高了可注入的泵浦功率,还通过光束净化特性实现了激光亮度增强。

关键词：光纤激光拉曼振荡器受激拉曼散射渐变折射率光纤

VenomAttack: automated and adaptive activity hijacking in Android

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Frontiers of Computer Science 2023年第1期17卷 187-204页

作者： pu SUN Sen CHEN Lingling fan Pengfei GAO Fu SONG Min YANG School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China College of Intelligence and Computing Tianjin UniversityTianjin 300350China College of Cyber Science Nankai UniversityTianjin 300350China School of Computer Science Fudan UniversityShanghai 200438China

Activity hijacking is one of the most powerful attacks in Android. Though promising, all the prior activity hijacking attacks suffer from some limitations and have limited attack capabilities. They no longer pose security threats in recent Android due to the presence of effective defense mechanisms. In this work, we propose the first automated and adaptive activity hijacking attack, named VenomAttack, enabling a spectrum of customized attacks (e.g., phishing, spoofing, and DoS) on a large scale in recent Android, even the state-of-the-art defense mechanisms are deployed. Specifically, we propose to use hotpatch techniques to identify vulnerable devices and update attack payload without re-installation and re-distribution, hence bypassing offline detection. We present a newly-discovered flaw in Android and a bug in derivatives of Android, each of which allows us to check if a target app is running in the background or not, by which we can determine the right attack timing via a designed transparent activity. We also propose an automated fake activity generation approach, allowing large-scale attacks. Requiring only the common permission INTERNET, we can hijack activities at the right timing without destroying the GUI integrity of the foreground app. We conduct proof-of-concept attacks, showing that VenomAttack poses severe security risks on recent Android versions. The user study demonstrates the effectiveness of VenomAttack in real-world scenarios, achieving a high success rate (95%) without users’ awareness. That would call more attention to the stakeholders like Google.

关键词： Android activity hijacking Android security mobile security

Control of vertical phase separation in high performance non-fullerene organic solar cell by introducing oscillating stratification preprocessing

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Nano Research 2021年第5期14卷 1319-1325页

作者： Dayong Zhang pu fan Jinyu Shi Yifan Zheng Jian Zhong Junsheng Yu State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu610054China Shanghai Institute of Optics and Fine Mechanics Chinese Academy of SciencesShanghai201800China

Non-fullerene organic solar cell(NFOSC)has attracted tremendous attention due to their great potential for commercial *** improve its power conversion efficiency(PCE),generally,sequential solution deposition(SSD)methods have been employed to construct the graded vertical phase separation(VPS)of the bulk-heterojunction(BHJ)active layer for efficient exciton separation and charge ***,a variety of orthogonal solvents used in the SSD may lead to the unpredicted change in the BHJ morphology and introduce additional defects inside the BHJ bulk thus complicate the fabrication ***,a simple oscillating stratification preprocessing(OSP)is developed to facilitate the formation of graded VPS among the BHJ *** a result,a significant improvement is obtained in PCE from 10.96%to 12.03%,which is the highest value reported among PBDB-T:ITIC based NFOSC.

关键词： oscillating stratification preprocessing graded vertical phase separation non-fullerene organic solar cells high performance sequential solution deposition

Characterization of bacteriophage BUCT631 lytic for K1 Klebsiella pneumoniae and its therapeutic efficacy in Galleria mellonella larvae

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Virologica Sinica 2023年第5期38卷 801-812页

作者： Pengjun Han Mingfang pu Yahao Li Huahao fan Yigang Tong College of Life Science and Technology Beijing University of Chemical TechnologyBeijing100029China Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing100029China

Severe infections caused by multidrug-resistant Klebsiella pneumoniae(***)highlight the need for new therapeutics with activity against this *** therapy is an alternative treatment approach for multidrug-resistant *** ***,we report a novel bacteriophage(phage)BUCT631 that can specifically lyse capsule-type K1 *** characterization revealed that phage BUCT631 could rapidly adsorb to the surface of *** and form an obvious halo ring,and it had relatively favorable thermal stability(4–50C)and pH tolerance(pH?4–12).In addition,the optimal multiplicity of infection(MOI)of phage BUCT631 was 0.01,and the burst size was approximately 303 PFU/*** analysis showed that phage BUCT631 has double-stranded DNA(total length of 44,812 bp)with a G t C content of 54.1%,and the genome contains 57 open reading frames(ORFs)and no virulence or antibiotic resistance related *** on phylogenetic analysis,phage BUCT631 could be assigned to a new species in the genus Drulisvirus of the subfamily *** addition,phage BUCT631 could quickly inhibit the growth of *** within 2 h in vitro and significantly elevated the survival rate of *** infected Galleria mellonella larvae from 10%to 90%in *** studies suggest that phage BUCT631 has promising potential for development as a safe alternative for control and treatment of multidrug-resistant *** infection.

关键词： Bacteriophage BUCT631 Klebsiella pneumoniae Genomic analysis Phage therapy Galleria mellonella

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陕北某气田集气站管线腐蚀机理研究

热加工工艺 2024年第6期53卷 128-132,137页

作者：杨开利张盼盼蒲阳峰李磊范鑫白海涛陕西延长石油(集团)责任有限公司延长气田采气二厂陕西榆林718500 西安石油大学石油工程学院西安石油大学陕西省油气田特种增产技术重点实验室陕西西安710065

针对陕北某气田集气站腐蚀严重的问题,对集气站接收的天然气和采出水组成进行分析。通过失重法测定L245NS碳钢在集气站不同部位的平均腐蚀速率,并对腐蚀产物膜和点蚀坑进行表征。结果表明:接收的天然气中CO_(2)含量与H_(2)S含量分别为6.... 详细信息

针对陕北某气田集气站腐蚀严重的问题,对集气站接收的天然气和采出水组成进行分析。通过失重法测定L245NS碳钢在集气站不同部位的平均腐蚀速率,并对腐蚀产物膜和点蚀坑进行表征。结果表明:接收的天然气中CO_(2)含量与H_(2)S含量分别为6.33%和354mg/m^(3),接收的采出水pH低,Cl^(-)和成垢阳离子含量高,接收的介质属于强腐蚀强结垢介质;进站管线、污水管线和出站管线的腐蚀产物膜均不完整,腐蚀程度分别为轻度、严重、轻度,腐蚀形态均为点蚀,主要以CO_(2)腐蚀为主。进站管线内的腐蚀结垢产物组成主要为BaSO_(4)和CaCO_(3),污水管线内的腐蚀结垢产物主要组成为单质S、FeCO_(3)和CaCO_(3)。造成点蚀的主要原因为腐蚀介质结垢严重,垢在管线表面分布不均匀及Cl^(-)和CO_(2)腐蚀。

关键词：集气站 L245NS碳钢管线腐蚀机理