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第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学

作者： shuguo fan Department of Chemistry and Life Science Chuxiong Normal University Institute for Bio-resources Development and Utilization in Central Yunnan Plateau

Background:Phalaenopsis equestris *** is a plant of the orchid genus *** ringspot virus(ORSV) is one of the two most prevalent viruses infecting orchids by mechanical transmission and no suitable control measurements are available thus *** on various plants and viruses show that overexpression of a virus CP in plants makes the plants confers resistance against this virus and close-related viruses. In this study,ORSV CP gene was introduced into *** ***’s genome,the factors affecting bombardment transformation of this Phalaenopsis species was investigated. Methods:*** ***,was used in this ***-like bodies(PLBs) were induced and proliferated in KC medium supplemented with 15%(v/v) coconut milk, 2%(w/v) sucrose and 3 mg/L *** were subcultured into fresh medium every 2 weeks. The optimal concentrations of MSO for selection were determined by plating 150 wild-type *** *** onto the solid media without phytohormones,and with various concentrations of MSO(0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,1.0 uM) *** generate the vector pORSV-CP used for genetic transformation in this study,the coding region of ORSV-CP was cloned into a pGreen 0229-35S binary vector under the control of two CaMV 35S *** transformation was done by particle bombardment and putative transformants were selected with *** and PCR southern have been done for the putative transformants. Results:Gene transformation was performed by biolistic bombardment with a 3-day recovery period on MSO-free medium and two selection stages on media with increasing amounts of selection agent,using concentrations of 0.5 and 2 uM *** gas pressure of 1350 psi and a distance of 9 cm from the stopping screen to orchid PLBs on plates were optimal parameters for biolistic bombardment of *** ***,as transformation under these parameters gave the highest number of putative transformants after 3 month

关键词： Odontoglossum ringspot virus(ORSV) Phalaenopsis equestris var.alba coat protein(CP)

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Genetic Engineering of CymMV-resistant Phalaenopsis

Genetic Engineering of CymMV-resistant Phalaenopsis

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第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学

作者： shuguo fan Department of Chemistry and Life Science Chuxiong Normal University Institute for Bio-resources Development and Utilization in Central Yunnan Plateau

Background:The orchid has been one of the most economic floricultural crops in China. Among them,Phalaenopsis *** first place in commercial value in the orchid industry. However,viral infections result in the great reduction in both quality and quantity of flowers, hence becoming a limiting factor in the orchid *** mosaic virus(CymMV) is one of the most prevalent plant viruses in *** aim of this research is to generate CymMV-resistant Phalaenopsis amabilis overexpressing either the sense or anti-sense strand of the CymMV coat protein(CP) genes using particle ***,the transgenic orchids will be resistant against viral infection. Methods:Calli have been induced from *** regeneration system has been established from *** gene comprising the capsid proteins of CymMV has been constructed,optimum conditions for particle bombardment have been *** calli and transgenic plantlets have been screened on selection *** selection medium was optimized for shoot induction with L-methionine sulfoximine(MSO) added as selection *** was done in both solid and liquid medium. Results:It was found that liquid media and experiment showed that selection in liquid medium was faster and more *** amplification of the inserted CP genes were used for genotyping,and PCR Southern assay confirm the insertion of anti-sense strand of CymMV CP genes into several lines of *** *** blot and Western blot have also been done for the putative transformed plants,and viral inoculation and detection will be performed on the transgenic Phalaenopsis when they grow to be big. Conclusions:Our results showed that the transgenic *** has been successfully created,and hopefully,the transgenic orchids will be resistant against viral infection.

关键词： Cymbididum mosaic virus(CymMV) Phalaenopsis amabilis coat protein(CP)

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Development of New Types of CMS and Restorer Lines from Rice Somaclones

Development of New Types of CMS and Restorer Lines from Rice...

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中国植物学会七十五周年年会

作者： fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

Young panicles from 11 rice materials were cultured on different media to induce calli and subsequent plant regeneration,followed by the examination of pollen fertility of regenerants to determine their characteristics of sterility and fertility.79 cases of male sterile variants in seven rice materials were found,in which 72 cases belonged to R generation (regenerated plants from

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Advances in Epidemiology and Disease Management Measures of Potato Late Blight

Advances in Epidemiology and Disease Management Measures of ...

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中国植物学会七十五周年年会

作者： fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

Potato is one of the most important crops in the *** blight is the most threat to potato production,and the pathogen of potato late blight is Phytophthora infestans (Mont.) de Bar3,.The origin,distribution,economic importance,symptoms,epidemiology,and disease management measures of late blight have been *** is discussed the future research to be conducted

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Investigation of BaMV Satellite RNA-encoded p20 in the Movement of satBaMV in p20 Transgenic Tobacco

Investigation of BaMV Satellite RNA-encoded p20 in the Movem...

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中国植物学会七十五周年年会

作者： fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

The satellite RNA of BaMV contains an open reading frame for a 20-kDa nonstructural protein,which facilitates the long distance movement of satBaMV in *** determine whether the frame shift mutant of satBaMV (F6) could...

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Agrobacterium-mediated Transformation of Oncidium Resistant to Virus Infection

Agrobacterium-mediated Transformation of Oncidium Resistant ...

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中国植物学会七十五周年年会

作者： fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

Oncidium is one of the most important commercial orchids around the world,but virus infection substantially decreases the quality and quantity of *** mosaic virus (CymMV) is the most common virus of *** generate the v...

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Response of Sodium Azide Induced Rice Mutants to BaMV

Response of Sodium Azide Induced Rice Mutants to BaMV

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中国植物学会七十五周年年会

作者： fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

Indirect ELISA,dot blot,and Northern blot have been done in order for investigation of sodium azide induced rice mutants’ response to Bamboo mosaic virus (BaMV) multiplication and *** mutants have variable responses ...

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高等植物成花分子机理研究进展

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楚雄师范学院学报 2014年第6期29卷 58-69页

作者：范树国楚雄师范学院化学与生命科学学院云南楚雄675000 滇中高原生物资源开发与利用研究所云南楚雄675000

花发育是有花植物生活史中一个极其重要而复杂的事件,它既涉及到细胞的分裂与分化,又涉及到组织和器官的形成,它既受环境因子的影响,又受自身生理信号的调控,它是一系列基因表达综合作用的结果。植物开花机制涉及四种成花诱导的途径:光... 详细信息

花发育是有花植物生活史中一个极其重要而复杂的事件,它既涉及到细胞的分裂与分化,又涉及到组织和器官的形成,它既受环境因子的影响,又受自身生理信号的调控,它是一系列基因表达综合作用的结果。植物开花机制涉及四种成花诱导的途径:光周期途径、春化途径、赤霉素途径和自主促进途径。但不同日长的植物,开花机制不同;同一日长类型的植物,开花机制也可能有差异。相对于草本植物,木本植物开花机理的研究起步较晚,尚未见茶花开花分子机制的研究报道。

关键词：开花时间基因光周期途径春化途径赤霉素途径自主促进途径

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Influence Factors on Callus Induction of Phalaenopsis

Influence Factors on Callus Induction of Phalaenopsis

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中国植物学会七十五周年年会

作者： Hu Chao fan shuguo (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000)

The effects of NAA,6-BA,AC and CM on callus induction of Phalaenopsis *** Gleam×*** Rose-Samson D×f001 (1296) were *** were conducted by using orthogonal *** were as follows:(1) As NAA 2.0 mg/L+6-BA 0.5 mg/L+AC

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水稻离体培养育性恢复突变的遗传学分析

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中国农业科学 1999年第5期32卷 95-97页

作者：范树国梁承邺刘鸿先中国科学院华南植物研究所!广州510650

Two fertile revertants were found in R 1 regenerated plants of Zhenshan 97A, one was R type restoration, the other was B type restoration. Three fertile revertants were found in R 1 regenerated plants of Hongyuan A and Baoyuan A, respectively. One excellent restorer line was obtained from the fertile revertants of Baoyuan A and Hongyuan A by means of genealogical method, respectively.

关键词：水稻离体培养育性恢复突变

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