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从新生儿分离到1.ST5型单增李斯特菌的药敏及基因组特征分析

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中国人兽共患病学报 2024年第7期40卷 644-651页

作者：刘增宾刘力李志荣许彩红王红彬杨如刚樊涛赵建宏张敬蕊河北医科大学第二医院石家庄050000 石家庄市第四医院石家庄050000

目的对1.新生儿感染ST5型单增李斯特菌LK1.0进行全基因组测序和耐药分析。方法从新生儿感染患者胃液标本中分离到1.疑似单增李斯特菌,采用VITEK2.Compact全自动微生物鉴定仪和1.S RNA序列测定进行鉴定,对鉴定的菌株应用E-test法进行5... 详细信息

目的对1.新生儿感染ST5型单增李斯特菌LK1.0进行全基因组测序和耐药分析。方法从新生儿感染患者胃液标本中分离到1.疑似单增李斯特菌,采用VITEK2.Compact全自动微生物鉴定仪和1.S RNA序列测定进行鉴定,对鉴定的菌株应用E-test法进行5种药物敏感性试验,同时利用三代测序平台对菌株进行全基因组测序,获得菌株基因组序列。基于基因组序列利用CARD抗生素耐药基因数据库获得菌株的耐药元件。基因组序列与巴斯德数据库中的单增李斯特菌的数据进行比对,获得菌株的多位点序列分型(multilocus sequence typing,MLST)、血清分型;同时利用毒力分析网站对菌株的毒力基因分布进行分析。利用前噬菌体数据库,在线进行菌株噬菌体的预测和注释。结果从新生儿患者分离到的菌株(LK1.0)鉴定为单增李斯特菌,该菌株对青霉素、氨苄西林、美罗培南、红霉素及复方新诺明等5种抗生素均敏感,其MLST分型为ST5型,血清型为1.2.-3b,染色体基因组全长为3032.82.bp,GC含量为37.91.,包含一条完整的环状质粒,序列长度为52.2. bp,携带liPI-1.力岛,且具有完整的InlA蛋白。固有耐药相关基因主要在染色体,1.SSI-1.力生存岛和1.LGI2.因组岛;LK1.0基因组中共预测到5个前噬菌体序列。结论本研究综合分析说明了ST5型临床单增李斯特菌的基因组特征和抗生素敏感性特点,为后期临床单增李斯特菌的感染治疗及其致病机理的研究提供数据支持。

关键词：新生儿单增李斯特菌表型药敏全基因组测序

Structure,ferroelectric,and enhanced fatigue properties of sol–gel-processed new Bi-based perovskite thin films of Bi(Cu_(1.2.Ti_(1.2.)O_(3)–PbTiO_(3)

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Chinese Physics B 2024年第5期33卷 608-615页

作者：宋伟宾席国强潘昭刘锦叶旭斌刘哲宏王潇单鹏飞张林兴鲁年鹏樊龙龙秦晓梅龙有文 Department of Physics Shanghai Normal UniversityShanghai 200234China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China Institute for Advanced Materials Technology University of Science and Technology BeijingBeijing 100083China Songshan Lake Materials Laboratory Dongguan 523808China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Bi-based perovskite ferroelectric thin films have wide applications in electronic devices due to their excellent ferroelectric *** Bi-based perovskite thin films Bi(Cu_(1.2.Ti_(1.2.)O_(3)–PbTiO_(3)(BCT–PT) are deposited on Pt(1.1./Ti/SiO_(2./Si substrates in the present study by the traditional sol–gel *** structures and related ferroelectric and fatigue characteristics are studied *** BCT–PT thin films exhibit good crystallization within the phase-pure perovskite structure,besides,they have a predominant(1.0) orientation together with a dense and homogeneous *** remnant polarization(2._(r)) values at 30 μC/cm^(2. and 1. μC/cm^(2. are observed in 0.1.CT–0.9PT and 0.2.CT–0.8PT thin films,*** intriguingly,although the polarization values are not so high,0.2.CT–0.8PT thin films show outstanding polarization fatigue properties,with a high switchable polarization of 93.6% of the starting values after 1.^(8) cycles,indicating promising applications in ferroelectric memories.

关键词： ferroelectric thin films perovskite PbTiO_(3)-BiMeO_(3)

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低剂量抗凝成功治疗高危肺栓塞1.

中华结核和呼吸杂志 2024年第5期47卷 450-454页

作者：范盛心蔡奇宴王晓慧李琦陈虹重庆医科大学附属第一医院呼吸与危重症医学科重庆400016 重庆医科大学附属第一医院放射科重庆400016

再灌注治疗被认为是治疗高危肺栓塞的基石,然而当合并溶栓禁忌且无法进行外科或介入干预时,高危肺栓塞的治疗就显得十分棘手。目前尚未见关于使用低剂量抗凝成功救治高危肺栓塞的报道。2.2.0}1.月30日重庆医科大学附属第一医院急诊科收... 详细信息

再灌注治疗被认为是治疗高危肺栓塞的基石,然而当合并溶栓禁忌且无法进行外科或介入干预时,高危肺栓塞的治疗就显得十分棘手。目前尚未见关于使用低剂量抗凝成功救治高危肺栓塞的报道。2.2.0}1.月30日重庆医科大学附属第一医院急诊科收治1.56岁男性自发性动脉瘤性蛛网膜下腔出血患者,因存在手术指征转入神经外科。入院第2.,在进行动脉瘤夹闭术过程中患者突发休克,完善D-二聚体、心肌损伤标志物、超声心动图、计算机断层扫描肺动脉造影等检查后考虑高危肺栓塞。因患者合并溶栓禁忌,且拒绝行介入治疗,最终通过低剂量抗凝联合血管活性药物成功将其治愈。

关键词：高危肺栓塞血管活性药物心肌损伤标志物计算机断层扫描动脉瘤夹闭术肺动脉造影急诊科再灌注治疗

刺梨果渣生物炭对白菜产量及品质和土壤性质的影响

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环境科学 2024年第6期45卷 3543-3552页

作者：樊洪谢珊龙天雨杨丽段淳刘元生宋理洪陈祖拥贵州大学农学院贵阳550025

为探明刺梨果渣生物炭对白菜产量及品质和土壤性质的影响,实现刺梨果渣的资源化利用,通过盆栽试验设置5个生物炭施用量:0%(CK)、1.(T1.、3%(T2.、5%(T3)和7%(T4),研究刺梨果渣生物炭施用对白菜产量及品质和土壤性质的影响.结果表明,①... 详细信息

为探明刺梨果渣生物炭对白菜产量及品质和土壤性质的影响,实现刺梨果渣的资源化利用,通过盆栽试验设置5个生物炭施用量:0%(CK)、1.(T1.、3%(T2.、5%(T3)和7%(T4),研究刺梨果渣生物炭施用对白菜产量及品质和土壤性质的影响.结果表明,①施用刺梨果渣生物炭能够显著提高白菜产量及品质,在5%生物炭施用量时效果最佳,白菜产量、可溶性固形物、可溶性糖、维生素C、全氮、全磷和全钾含量较CK分别提升了71.51.、40.1.%、33.65%、38.08%、9.03%、2..85%和35.38%;②施用刺梨果渣生物炭能够显著改善土壤性质提高土壤养分含量及有效性,在5%生物炭施用量时效果较好,土壤pH、有机质、全氮、碱解氮、有效磷和速效钾含量较CK分别增加了41.06%、1.4.84%、1.7.48%、1.0.79%、341.75%和62..1.%,有效Fe、Mn、Cu、Zn含量和交换性Ca、Mg含量较CK分别提高37.68%、61.69%、400.00%、4 648.84%、61..1.%和351.42.;③施用刺梨果渣生物炭能够显著增强土壤酶活性,土壤脲酶、酸性磷酸酶、过氧化氢酶和蔗糖酶分别较CK处理提高的范围为51.43%~362.86%、90.63%~1.4.1.%、2.0}40%~85.1.%和82.92.~2.0}.43%;④冗余分析表明施用刺梨果渣生物炭后,土壤有效钾、交换性钙、有机质、有效磷和有效锌等是白菜产量及品质变化的主要影响因子,与其呈显著正相关关系.综上所述,施用刺梨果渣生物炭可以显著提高白菜产量及品质,改善土壤性质,将刺梨果渣制备成生物炭可以为刺梨果渣资源化合理利用提供理论参考.

关键词：刺梨果渣生物炭白菜产量品质土壤性质冗余分析

New Record Ocean Temperatures and Related Climate Indicators in 2.2.

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Advances in Atmospheric Sciences 2024年第6期41卷 1068-1082页

作者： lijing CHENG John ABRAHAM Kevin E.TRENBERTH Tim BOYER Michael EMANN Jiang ZHU fan WANG Fujiang YU Ricardo LOCARNINI John FASULLO Fei ZHENG Yuanlong li {1. ZHANG liying WAN Xingrong CHEN Dakui WANG licheng FENG Xiangzhou SONG Yulong liU Franco RESEGHETTI Simona SIMONCELli Viktor GOURETSKI Gengxin CHEN Alexey MISHONOV Jim REAGAN Karina VON SCHUCKMANN Yuying PAN Zhetao TAN Yujing ZHU Wangxu WEI Guancheng li qiuping REN lijuan CAO Yayang LU International Center for Climate and Environment Sciences Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China university of St.Thomas School of EngineeringMinnesota 55105USA NSF National Center for Atmospheric Research BoulderColorado 80307USA university of Auckland Auckland 1010New Zealand National Oceanic and Atmospheric Administration National Centers for Environmental InformationSilver SpringMaryland 20910USA Department of Earth and Environmental Science University of PennsylvaniaPhiladelphiaPennsylvania 19104USA Institute of Oceanology Chinese Academy of SciencesQingdao 266071China National Marine Environmental Forecasting Center Ministry of Natural Resources of ChinaBeijing 100081China Oceanographic Data Center Chinese Academy of SciencesQingdao 266071China College of Oceanography Hohai UniversityNanjing 210098China National Marine Data and Information Service Tianjin 300171China Italian National Agency for New Technologies Energy and Sustainable Economic DevelopmentS.Teresa Research CenterLerici 19032Italy Istituto Nazionale di Geofisica e Vulcanologia Sede di BolognaBologna 40128Italy South china Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301China ESSIC/CISESS-MD University of MarylandCollege ParkMD20740USA Mercator Ocean International Toulouse 31400France Eco-Environmental Monitoring and Research Center Pearl River Valley and South China Sea Ecology and Environment AdministrationMinistry of Ecology and EnvironmentPRCGuangzhou 510611China National Meteorological Information Center China Meteorological AdministrationBeijing 100081China International Research Center of Big Data for Sustainable Development Goals Beijing 100094China

The global physical and biogeochemical environment has been substantially altered in response to increased atmospheric greenhouse gases from human *** 2.2.,the sea surface temperature(SST)and upper 2.00 m ocean heat content(OHC)reached record *** 0–2.00 m OHC in 2.2. exceeded that of 2.2. by 1.±1. ZJ(1.Zetta Joules=1.2.0}Joules)(updated IAP/CAS data);9±5 ZJ(NCEI/NOAA data).The Tropical Atlantic Ocean,the Mediterranean Sea,and southern oceans recorded their highest OHC observed since the *** with the onset of a strong El Niño,the global SST reached its record high in 2.2. with an annual mean of~0.2.℃ higher than 2.2. and an astounding>0.3℃ above 2.2. values for the second half of *** density stratification and spatial temperature inhomogeneity indexes reached their highest values in 2.2..

关键词： ocean heat content salinity stratification global warming climate

A novel deep generative model for mRNA vaccine development: Designing 5′ UTRs with N1.methyl-pseudouridine modification

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1814-1826页

作者： Xiaoshan Tang Miaozhe Huo Yuting Chen Hai Huang Shugang qin Jiaqi Luo Zeyi qin Xin Jiang Yongmei liu Xing Duan Ruohan Wang lingxi Chen Hao li Na fan Zhongshan He Xi He Bairong Shen Shuai Cheng li Xiangrong Song Institute of Systems Genetics Department of Critical Care MedicineFrontiers Science Center for Disease-related Molecular NetworkState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610000China Department of Computer Science City University of Hong KongHong Kong 99907China Department of Biology Brandeis UniversityBostonMA 02453USA

Efficient translation mediated by the 5'untranslated region(5'UTR)is essential for the robust efficacy of mRNA ***,the N1.methyl-pseudouridine(m1.modification of mRNA can impact the translation efficiency of the 5'*** discovered that the optimal 5'UTR for m1.-modified mRNA(m1.-5'UTR)differs significantly from its unmodified counterpart,high-lighting the need for a specialized tool for designing mly-5'UTRs rather than directly utilizing high-expression endogenous gene 5'*** response,we developed a novel machine learning-based tool,Smart5UTR,which employs a deep generative model to identify superior m1.-5'UTRs in *** tailored loss function and network architecture enable Smart5UTR to overcome limitations inherent in existing *** a result,Smart5UTR can successfully design superior 5'UTRs,greatly benefiting mRNA vaccine ***,Smart5UTR-designed superior 5'UTRs significantly enhanced antibody titers induced by COVID-1. mRNA vaccines against the Delta and Omicron variants of SARS-CoV-2.surpassing the performance of vaccines using high-expression endogenous gene 5'UTRs.

关键词： mRNA vaccine Machine learning 5'UTR mRNA design Sequence design N1.Methyl-pseudouridine COVID-1. SARS-CoV-2

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主导产业对黑龙江省经济增长的影响

学习与探索 2004年第4期 90-93页

作者：范海燕李洪山哈尔滨工业大学管理学院黑龙江哈尔滨150001 东北林业大学经济管理学院黑龙江哈尔滨150040

黑龙江省的产业层次低,主导产业对经济增长的带动作用小。主导产业的发展水平决定着区域经济发展的程度和水平。为此,必须从实际出发促进主导产业发展,加大体制创新和技术创新的力度,产业集群是提高区域经济竞争力的一项非常有效的发展... 详细信息

黑龙江省的产业层次低,主导产业对经济增长的带动作用小。主导产业的发展水平决定着区域经济发展的程度和水平。为此,必须从实际出发促进主导产业发展,加大体制创新和技术创新的力度,产业集群是提高区域经济竞争力的一项非常有效的发展模式。

关键词：经济增长主导产业产业结构黑龙江省

Reference values of carotid intima-media thickness and arterial stiffness in Chinese adults based on ultrasound radio frequency signal:A nationwide,multicenter study

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Chinese Medical Journal 2024年第15期137卷 1802-1810页

作者： Changyang Xing Xiujing Xie Yu Wu Lei Xu Xiangping Guan fan li Xiaojun Zhan Hengli Yang Jinsong li {1. Zhou Yuming Mu {1.ng Zhou Yunchuan Ding Yingli Wang Xiangzhu Wang Yu Zheng Xiaofeng Sun Hua li Chaoxue Zhang Cheng Zhao Shaodong {1.u Guozhen Yan hong Yang Yinjuan Mao Weiwei Zhan Chunyan Ma Ying Gu Wu Chen Mingxing Xie Tianan Jiang lijun Yuan Department of Ultrasound Medicine Tangdu HospitalAir Force Medical UniversityXi’anShaanxi 710038China Department of Ultrasound Medicine The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China Department of Ultrasound Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Ultrasound Xi’an Hospital of Traditional Chinese MedicineXi’anShaanxi 710021China Ultrasound Medical Diagnosis Center Shaanxi Provincial People’s HospitalXi’anShaanxi 710068China Department of Ultrasound Hospital of Northwestern Polytechnical UniversityXi’anShaanxi 710072China Department of Ultrasound Xi’an No.1 HospitalThe First Affiliated Hospital of Northwest UniversityXi’anShaanxi 710002China Department of Ultrasound Diagnosis The Second Affiliated HospitalXi’an Medical CollegeXi’anShaanxi 710038China Department of Ultrasound Xi’an Gem Flower ChangQing HospitalXi’anShaanxi 710201China Department of Ultrasound The Second Affiliated Hospital of Xi’an Jiaotong UniversityChinaXi’anShaanxi 710004China Department of Echocardiography First Affiliated Hospital of Xinjiang Medical UniversityUrumqiXinjiang 830011China Department of Ultrasound Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Ultrasound Yan’an HospitalThe Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650051China Department of Ultrasound Yan’an University Xianyang HospitalXianyangShaanxi 712000China Department of Function Examination Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China Department of Ultrasound Xi’an Central HospitalXi’anShaanxi 710004China Cadre’s Wards Ultrasound Department Diagnostic Ultrasound CenterFirst Hospital of Jilin UniversityChangchunJilin 130000China Department of Echocardiography Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical UniversityUrumqiXinjiang 830002China Department of Ultrasound First Affiliated

Background:Carotid intima-media thickness(IMT)and diameter,stiffness,and wave reflections,are independent and important clinical biomarkers and risk predictors for cardiovascular *** purpose of the present study was to establish nationwide reference values of carotid properties for healthy Chinese adults and to explore potential clinical ***:A total of 3053 healthy Han Chinese adults(1.2. women)aged 1.-79 years were enrolled at 2. collaborating tertiary centers throughout china between April 2.2.3}and July *** real-time tracking of common carotid artery walls was achieved by the radio frequency(RF)ultrasound *** IMT,diameter,compliance coefficient,βstiffness,local pulse wave velocity(PWV),local systolic blood pressure,augmented pressure(AP),and augmentation index(AIx)were then automatically measured and *** were stratified by age groups and *** relationships between age and carotid property parameters were analyzed by Jonckheere-Terpstra test and simple linear *** major clinical determinants of carotid properties were identified by Pearson’s correlation,multiple linear regression,and analyses of ***:All the parameters of carotid properties demonstrated significantly age-related *** showed thinner IMT,smaller carotid diameter,larger AP,and AIx than ***βstiffness and PWV were significantly higher in men than women before forties,but the differences reversed after *** increase rate of carotid IMT(5.5µm/year in women and 5.8µm/year in men)and diameter(0.03 mm/year in both men and women)were similar between men and *** the stiffness and wave reflections,women showed significantly larger age-related variations than men as demonstrated by steeper regression slopes(all P for age by sex interactionlinical determinants of carotid properties with adjustment of age and ***

关键词： Intima-media thickness Arterial stiffness Reference values Ultrasound radio frequency signal Wave reflections

N Accumulation and Translocation in Four Japonica Rice Cultivars at Different N Rates

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Pedosphere 2007年第6期17卷 792-800页

作者： ZHANG Yao-hong {1. Jian-Bo ZHANG Ya-{2. WANG Dong-Sheng HUANG qi-Wei SHEN qi-Rong College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 （China）

Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive *** experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast *** Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2.04 with three N rates of 0,60,and 1.0 kg N ha^(-1..Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and *** yields exhibited significant differences (P2.cation *** N rates improved N uptake at anthesis and maturity in all four cultivars(P1.and N-translocation efficiency from 33% to 68%. Grain yield was {2.near to N uptake at anthesis(r^2.0.78^(**))and N translocation(r^2.0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N ferti{2.zer supply in southeast china should efficiently increase N-recovery rate while maintaining grain yield and soil ferti{2.ty.

关键词： rain yield Japonica rice cultivar N accumulation N application rate N translocation