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环磷酰胺致大鼠弱精症的代谢组学特征

现代生物医学进展 2025年第1期25卷 26-35页

作者：宋嘉琳魏雅岚俞东红范小路王心睿福建省妇幼保健院医学研究中心

目的：观察环磷酰胺致大鼠弱精症模型（CP）与正常组大鼠的代谢差异，探索其潜在的代谢特征及通路机制。方法：用环磷酰胺（30 mg/kg）连续腹腔注射7天，建立大鼠弱精症模型；通过精子计数来检测精子浓度和活力；采用HE染色观察睾丸形... 详细信息

目的：观察环磷酰胺致大鼠弱精症模型（CP）与正常组大鼠的代谢差异，探索其潜在的代谢特征及通路机制。方法：用环磷酰胺（30 mg/kg）连续腹腔注射7天，建立大鼠弱精症模型；通过精子计数来检测精子浓度和活力；采用HE染色观察睾丸形态；采用液相色谱-质谱联用（LC-MS）非靶向代谢组学检测两组间的代谢差异。采用试剂盒检测血清中超氧化物歧化酶（SOD）、脂质氧化酶（MDA）、总谷胱甘肽的浓度。结果：与正常组相比，环磷酰胺组的精子浓度降低，精子活力下降。HE染色结果表明，环磷酰胺组的各级生精细胞排列紊乱，数量较正常组显著减少。提示弱精症大鼠模型建立成功。代谢组学分析结果显示两组代谢轮廓分离趋势较好，共筛选出129种差异代谢物。对差异代谢物进行KEGG通路富集分析，结果显示胆碱代谢、甘油磷脂代谢及鞘脂信号通路等代谢通路在两组间具有显著差异。KEGG鞘脂信号通路图显示活性氧（ROS）参与了该信号通路调控。弱精组中血清SOD和总谷胱甘肽浓度下降，MDA浓度增高，差异具有统计学意义。结论：弱精症的发生可能与其抗氧化水平下降，脂质氧化水平升高有关，过度的氧化应激可能破坏精子膜结构的完整性，对精子DNA造成一定的损伤，从而降低精子的数量和活力，其机制与鞘脂代谢通路密切相关。

关键词：弱精症代谢组学氧化应激环磷酰胺活性氧

The effect of fine grits and fine debris concentrations on the MLVSS/MLSS ratio of an activated sludge system

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Journal of Environmental Sciences 2025年第1期147卷 607-616页

作者： Zhao Jiang Zhiheng Xia Shuai Liu QiWei Haitao fan lu Qi Guohua Liu Hongchen Wang Low-carbon Water Environment Technology Research Center School of Environment&Natural ResourcesRenmin University of ChinaBeijing 100872China

This study embarks on an explorative investigation into the effects of typical concentrations and varying particle sizes of fine grits(FG,the involatile portion of suspended solids)and fine debris(FD,the volatile yet unbiodegradable fraction of suspended solids)within the influent on themixed liquor volatile suspended solids(MLVSS)/mixed liquor suspended solids(MLSS)ratio of an activated sludge *** meticulous experimentation,it was discerned that the addition of FG or FD,the particle size of FG,and the concentration of FD bore no substantial impact on the pollutant removal efficiency(denoted by the removal rate of COD and ammonia nitrogen)under constant operational ***,a notable decrease in the MLVSS/MLSS ratio was observed with a typical FG concentration of 20 mg/L,with smaller FG particle sizes exacerbating this ***,variations in FD concentrations influenced both MLSS andMLVSS/MLSS ratios;a higher FD concentration led to an increased MLSS and a reduced MLVSS/MLSS ratio,indicating FD accumulation in the system.A predictive model for MLVSS/MLSS was constructed based on quality balance calculations,offering a tool for foreseeing the MLVSS/MLSS ratio under stable long-term influent conditions of FG and *** model,validated using data from the BXH wastewater treatment plant(WWTP),showcased remarkable accuracy.

关键词： Fine grits and fine debris Activated sludge system MLVSS/MLSS ratio Predictive model

Current situation and prospect of artificial intelligence application in endoscopic diagnosis of Helicobacter pylori infection

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Artificial Intelligence in Gastrointestinal Endoscopy 2021年第3期2卷 50-62页

作者： Yi-fan lu Bin Lyu Department of Gastroenterology The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina

With the appearance and prevalence of deep learning,artificial intelligence(AI)has been broadly studied and made great progress in various fields of medicine,including *** pylori(***),closely associated with various digestive and extradigestive diseases,has a high infection rate *** surveillance can evaluate *** infection situations and predict the risk of gastric cancer,but there is no objective diagnostic criteria to eliminate the differences between *** computer-aided diagnosis system based on AI technology has demonstrated excellent performance for the diagnosis of *** infection,which is superior to novice endoscopists and similar to *** with the visual diagnosis of *** infection by endoscopists,AI possesses voluminous advantages:High accuracy,high efficiency,high quality control,high objectivity,and high-effect *** review summarizes the previous and recent studies on AI-assisted diagnosis of *** infection,points out the limitations,and puts forward prospect for future research.

关键词： Artificial intelligence Helicobacter pylori Endoscopy Diagnosis Deep learning Machine learning

Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation

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World Journal of Gastroenterology 2017年第6期23卷 986-998页

作者： Li-Jia Bu Han-Qing Yu lu-lu fan Xiao-Qiu Li fang Wang Jia-Tao Liu Fei Zhong Cong-Jun Zhang Wei Wei Hua Wang Guo-Ping Sun Department of Oncology the First Affiliated Hospital of Anhui Medical University Department of Pharmacy the First Affiliated Hospital of Anhui Medical University Institute of Clinical Pharmacology Anhui Medical University Institute for Liver Diseases of Anhui Medical University

AIM To clarify the mechanisms involved in the critical endoplasmic reticulum(ER) stress initiating unfolded protein response pathway modified by *** Hepatoma cells, Hep G2, were cultured in vitro. Flow cytometry and TUNEL assay were used to measure Hep G2 cell apoptosis. Western blotting and quantitative reverse transcription-polymerase chain reaction methods were used to determine the protein and messenger RNA levels of ER stress and apoptosis related genes' expression, respectively. Tissue microarray construction from patients was verified by immunohistochemical *** In the present study, we first identified that melatoninselectively blocked activating transcription factor 6(ATF-6) and then inhibited cyclooxygenase-2 (COX-2) expression, leading to enhanced liver cancer cell apoptosis under ER stress condition. Dramatically increased CCAAT-enhancer-binding protein homologous protein level, suppressed COX-2 and decreased Bcl-2/Bax ratio by melatonin or ATF-6 si RNA contributed the enhanced Hep G2 cell apoptosis under tunicamycin (an ER stress inducer) stimulation. In clinical hepatocellular carcinoma patients, the close relationship between ATF-6 and COX-2 was further *** These findings indicate that melatonin as a novel selective ATF-6 inhibitor can sensitize human hepatoma cells to ER stress inducing apoptosis.

关键词： Melatonin Endoplasmic reticulum stress Activating transcription factor 6 Cyclooxygenase-2 Hepatocellular carcinoma

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非线性协整模型及其状态监测应用研究

振动工程学报 2011年第5期24卷 468-472页

作者：鲁帆陈前南京航空航天大学振动工程研究所江苏南京210016

基于线性协整理论的液压舵面伺服系统状态监测已经取得了积极的效果,但显示出对部分故障敏感性较弱的不足。为提高协整模型监测效力,研究采用基于神经网络的非线性协整建模方法,建立非平稳系统的非线性协整关系监测模型。仿真研究证实,... 详细信息

基于线性协整理论的液压舵面伺服系统状态监测已经取得了积极的效果,但显示出对部分故障敏感性较弱的不足。为提高协整模型监测效力,研究采用基于神经网络的非线性协整建模方法,建立非平稳系统的非线性协整关系监测模型。仿真研究证实,该方法比线性协整监测模型具有更好的故障敏感性和可靠性。

关键词：状态监测非线性协整液压伺服系统神经网络

Differentiation of embryonic stem cells into corneal epithelium

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Science China(Life Sciences) 2005年第5期48卷 471-480页

作者： WANG Zhichong GE Jian HUANG Bing GAO Qianying LIU Bingqian WANG Linghua YU Ling fan Zhigang lu Xiaoming LIU Jingbo Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China

Our project was to determine whether embryonic stem (ES) cells could be induced to differentiate into corneal epithelia by superficial corneoscleral limbal stroma. To achieve this goal, ES-GFP cell line D3 was pre-induced by retinoic acid (RA). The pre-induced cells were seeded on deepithelialized superficial corneoscleral slices (SCSS) to form a monolayer, and di-vided into three groups. Group 1 was cultured and passaged in vitro for direct detection. Group 2 was exposed to air-liquid interfaces for 10 days and implanted into the subcutaneous layer of nude mice for 2 weeks for further induction in vivo. Group 3 was cultured in vitro without any in-ducing factors for control. There were no teratomas found in nude mice which were implanted with differentiated ES cells after two weeks. The differentiated cells showed an appearance of epithelia both in vitro and in vivo. Expression of CK3, P63 and PCNA was detected by immuno-histochemical staining in the differentiated cells in group 1 and 2. Microvillis and zonula oc-cludens were observed on the surface of the differentiated cells under an electron microscope. In the control group, ES cells differentiated freely without any inducing factors. Most cells were shed and formed a neuronal dendrite-like structure, and a minority of cells appeared polymorphic. These results demonstrate that ES cells can differentiate into corneal epithelia on the surface of SCSS under the controlled condition. Differentiated ES cells could be used as epithelial seeding cells for the reconstruction of ocular surface and corneal tissue engineering in the future.

关键词： embryonic stem cells, differentiation, corneal epithelium, microenvironment, superficial corneoscleral limbal stroma.

基于广义极值分布和Metropolis-Hastings抽样算法的贝叶斯MCMC洪水频率分析方法

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水利学报 2013年第8期44卷 942-949页

作者：鲁帆严登华中国水利水电科学研究院流域水循环模拟与调控国家重点实验室北京100038

广义极值(GEV)分布是国内外洪水频率分析建模中广泛应用的一种概率分布。本文将水文频率分布线型的未知参数看作随机变量,通过基于Metropolis-Hastings抽样算法的贝叶斯MCMC方法估计GEV分布参数和设计洪水的后验分布,并据此进行极值洪... 详细信息

广义极值(GEV)分布是国内外洪水频率分析建模中广泛应用的一种概率分布。本文将水文频率分布线型的未知参数看作随机变量,通过基于Metropolis-Hastings抽样算法的贝叶斯MCMC方法估计GEV分布参数和设计洪水的后验分布,并据此进行极值洪水的频率分析。汉江流域丹江口水库年最大1日(3日、5日、7日)洪量和年最大洪峰流量频率分析结果表明,基于Metropolis-Hastings抽样的MCMC模拟在GEV分布参数的贝叶斯估计计算中行之有效;由于利用了与似然函数渐近性质无关的先验信息,贝叶斯估计方法得到的高分位数设计洪量的后验分布比经典统计方法得到的设计洪量能包含更多的信息,从而能表达由于参数不确定性而引起的预测不确定性。该方法能显著地通过分位数图、PPCC法、均方根误差法、K-S法等多种拟合优度检验方法,拟合效果不亚于矩法、极大似然估计法等常用的经典统计方法。

关键词：洪水频率分析贝叶斯估计广义极值分布 Metropolis—Hastings抽样拟合优化度检验

Strengthening Mechanism and Microstructure of Deformable Ti Particles Reinforced AZ91 Composite

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Acta Metallurgica Sinica(English Letters) 2022年第12期35卷 1935-1945页

作者： Biao Tang Jian-Bo Li Jun-Liu Ye Huan luo Yi-Tao Wang Bo Guan Yang-fan lu Xian-Hua Chen Kai-Hong Zheng Fu-Sheng Pan School of Materials Science and Engineering Chongqing UniversityChongqing 400044China International Joint Laboratory for Light Alloys(MOE) School of Materials Science and EngineeringChongqing UniversityChongqing 400044China Institute of New Materials Guangdong Academy of SciencesGuangzhou 510650China

In this study,the deformable titanium(Ti)particles reinforced AZ91 composite was successfully prepared by powder metallurgy and subsequent *** mechanical properties and microstructural evolution of pure AZ91 and 5 Ti/AZ91 composite were *** yield strength,ultimate tensile strength,and elongation of 5 Ti/AZ91 composite are measured to be 212 MPa,323 MPa,and 10.1%,*** analysis revealed that Ti particles are elongated along the extrusion direction,forming a discontinuous strip Ti particles,fine precipitated Mg_(17)Al_(12) phase inhibits dynamic recrystallization(DRX)behavior through Zener pinning effect and hinders the growth of matrix grains,resulting in refiner grains of 5 Ti/AZ91 *** deformed Ti particles and magnesium(Mg)matrix to generate additional heterogeneous deformation-induced(HDI)*** deformation-induced strengthening mainly contributed to the increment of yield strength for 5 Ti/AZ91 composite.

关键词： Magnesium matrix composites Tiparticles Recrystallization Mechanical properties Heterogeneous deformation

Long non-coding RNAs as promising targets for controlling disease vector mosquitoes

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Insect Science 2025年第1期32卷 24-41页

作者： Yujiao Han Qian Pu Ting fan Tianqi Wei Yankun Xu lu Zhao Shiping Liu State Key Laboratory of Resource Insects Southwest UniversityChongqing400716 China

Hematophagous female mosquitoes are important vectors of numerous devastating human diseases,posing a major public health *** prevention and control of mosquito-borne diseases rely considerably on progress in understanding the molecular mechanisms of various life activities,and accordingly,the molecules that regulate the various life activities of mosquitoes are potential targets for implementing future vector control *** long non-coding RNAs(lncRNAs)have been identified in mosquitoes and significant progress has been made in determining their ***,we present a comprehensive overview of the research advances on mosquito lncRNAs,including their molecular identification,function,and interaction with other non-coding RNAs,as well as their synergistic regulatory roles in mosquito life *** also highlight the potential roles of competitive endogenous RNAs in mosquito growth and development,as well as in insecticide resistance and virus–host *** into the biological functions and mechanisms of lncRNAs in mosquito life activities,viral replication,pathogenesis,and transmission will contribute to the development of novel drugs and safe vaccines.

关键词： competitive endogenous RNAs(ceRNAs) insecticide resistance long noncoding RNA(lncRNA) mosquito mosquito-borne diseases pathogen-host interaction