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Expression of Recombinant Human FADD,Preparation of Its Polyclonal Antiserum and the Application in Immunoassays

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Cellular & Molecular Immunology 2008年第6期5卷 471-474页

作者： faiz MMT Marikar The State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Center of Hepatobiliary Diseases Nanjing University

The wild-type human Fas-associated death domain （FADD） protein was expressed as a His-tag fusion protein in Escherichia coll. Recombinant FADD proteins were purified under the denatured condition. After denatured protein purification, it was refolded and obtained at a yield of about 23 mg/L. Purified FADD exhibited as a homogenous band corresponding to the molecular weight of 31 kDa. Immunization of rabbits against the refolded FADD protein was allowed the production of high titre polyclonal antiserum. This new polyelonal antibody could recognize recombinant FADD protein in Western blot. Immunoreactivity was also observed in immunofiuorescence assay. The low cost polyclonal antiserum was applicable to extensive detection of FADD in various immunoassays. Cellular ＆ Molecular Immunology. 2008;5（6）：471-474.

关键词： FADD His-tag fusion protein polyclonal antibody immunofluorescence assay

Noise analysis of grating-based x-ray differential phase-contrast imaging with angular signal radiography

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Chinese Physics B 2017年第4期26卷 138-143页

作者： Wali faiz 鲍园高昆吴朝卫晨希昝贵彬朱佩平田扬超 National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China Shanghai United Imaging Healthcare Co.Ltd. Shanghai 201807China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

X-ray phase-contrast imaging is one of the novel techniques,and has potential to enhance image quality and provide the details of inner structures *** this work,we investigate quantitatively signal-to-noise ratio（SNR） of grating-based x-ray phase contrast imaging（GBPCI） system by employing angular signal radiography（ASR）.Moreover,photon statistics and mechanical error that is a major source of noise are investigated in *** show the dependence of SNR on the system parameters and the effects on the extracted absorption,refraction and scattering *** conclusions can be used to optimize the system design for upcoming practical applications in the areas such as material science and biomedical imaging.

关键词： angular signal radiography signal-to-noise ratio photon statistical noise mechanical error

Contemporary Trends in Power Electronics Converters for Charging Solutions of Electric Vehicles

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CSEE Journal of Power and Energy Systems 2020年第4期6卷 911-929页

作者： Salman Habib Muhammad Mansoor Khan Farukh Abbas Abdar Ali Muhammad Talib faiz Farheen Ehsan Houjun Tang School of Electronic Information and Electrical Engineeringand Key Laboratory of Control of Power Transmission and ConversionShanghai Jiao Tong UniversityShanghai 200240China Department of Electrical Engineering University of Engineering and TechnologyLahore 54890Pakistan School of International and Public Affairs Shanghai Jiao Tong UniversityShanghai 200240China

Electrifying the transport sector requires new possibilities for power electronics converters to attain reliable and efficient charging solutions for electric vehicles(EVs).With the continuous development in power electronics converters,the desire to reduce gasoline consumption and to increase the battery capacity for more electric range is achievable for EVs in the near *** main interface between the power network and EV battery system is a power electronics converter,therefore,there is a considerable need of new power converters with low cost and high reliability for the advance charging mechanism of *** rapid growth in power converter topologies brings substantial opportunities in EV charging *** view of this fact,this paper investigates the significant aspects,current progress,and challenges associated with several power converters to suggest further improvements in charging systems of *** particular,an extensive analysis of front-end as well as back-end converter configurations is ***,the comparative properties of resonant converter topologies along with other DCDC converters are discussed in ***,isolated,and non-isolated topologies with soft switching techniques are classified and rigorously analyzed with a view to their respective issues and *** is foreseen that this paper would be a valuable addition and a worthy source of information for researchers exploring the area of power converter topologies for charging solutions of EVs.

关键词： Batteries chargers converter topologies electric vehicles(EVs) power electronics plug-in hybrid electric vehicles(PHEVs)

Orthogonal Polynomials and Padé Approximation

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Advances in Manufacturing 1998年第2期2卷 24-27页

作者： faiz Ahmad (Department of Mathematics, Quaid i Azam University, Pakistan)

The diagonal Padé approximants for exp ( x ), tan x and tanh x are obtained in a simple manner by using the property of Legendre polynomials that on P r1 (x) is orthogonal to every polynomial of lo... 详细信息

关键词： orthogonal polynomials Padé approximation

Primary Multiple Cerebral Hydatid Cyst in 8 Year-Old Girl: A Rare Cause of Childhood Seizure

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Extra-Axial Anaplastic Pleomorphic Xanthoastrocytoma Mimicking Meningioma:A Case Report with Literature Review

Open Journal of Modern Neurosurgery（现代神经外科学进展） 2024年第2期 149页

作者： Kaoutar Stitou Ilias Zahir Oualid Mohammed Hmamouche Marouane Hammoud Faycal Lakhdar Mohammed Benzagmout Khalid Chakour Mohammed El faiz Chaoui

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Identification of Anomaly Scenes in Videos Using Graph Neural Networks

Open Journal of Modern Neurosurgery（现代神经外科学进展） 2024年第3期 203页

作者： Kaoutar Stitou Ilias Zahir Oualid Mohammed Hmamouche Marouane Hammoud Faycal Lakhdar Mohammed Benzagmout Khalid Chakour Mohammed El faiz Chaoui

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Computers, Materials & Continua 2023年第3期74卷 5417-5430页

作者： Khalid Masood Mahmoud M.Al-Sakhnini Waqas Nawaz Tauqeer faiz Abdul Salam Mohammad Hamza Kashif Department of Software Engineering Lahore Garrison UniversityLahore54000Pakistan School of Business Skyline University CollegeSharjahUAE Al-Madinah International University–Faculty of Computer and Information Technology Kuala LumpurMalaysia College of Computer and Information Systems Islamic University of MadinahMadinahKingdom of Saudi Arabia School of IT Skyline University CollegeSharjahUAE Faculty of Engineering Computing and Science(FECS)-Swinburne University of TechnologySarawak Department of General Education Skyline University CollegeUniversity City of SharjahUAE Department of Computer Science Lahore Garrison UniversityLahore54000Pakistan

Generally,conventional methods for anomaly detection rely on clustering,proximity,or *** themassive growth in surveillance videos,outliers or anomalies find ingenious ways to obscure themselves in the network and make conventional techniques *** research explores the structure of Graph neural networks(GNNs)that generalize deep learning frameworks to graph-structured *** node in the graph structure is labeled and anomalies,represented by unlabeled nodes,are predicted by performing random walks on the node-based graph *** to their strong learning abilities,GNNs gained popularity in various domains such as natural language processing,social network analytics and *** detection is a challenging task in computer vision but the proposed algorithm using GNNs efficiently performs the identification of *** Graph-based deep learning networks are designed to predict unknown objects and *** our case,they detect unusual objects in the form of malicious *** edges between nodes represent a relationship of nodes among each *** case of anomaly,such as the bike rider in Pedestrians data,the rider node has a negative value for the edge and it is identified as an *** encoding and decoding layers are crucial for determining how statistical measurements affect anomaly identification and for correcting the graph path to the best possible *** show that the proposed framework is a step ahead of the traditional approaches in detecting unusual activities,which shows a huge potential in automatically monitoring surveillance *** autonomous monitoring of CCTV,crime control and damage or destruction by a group of people or crowd can be identified and alarms may be triggered in unusual activities in streets or public *** suggested GNN model improves accuracy by 4%for the Pedestrian 2 dataset and 12%for the Pedestrian 1 dataset compared to a few state-of the-art techniq

关键词： Graph neural network deep learning anomaly detection auto encoders

Antibacterial mechanism with consequent cytotoxicity of different reinforcements in biodegradable magnesium and zinc alloys: A review

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Journal of Magnesium and Alloys 2023年第9期11卷 3038-3058页

作者： Chowdhury Ahmed shahed faiz Ahmad Ebru Günister Farhana Mohd Foudzi Saad Ali Khurshid Malik Wan Sharuzi Wan Harun Department of Mechanical Engineering Universiti Teknologi PETRONAS(UTP)32610Seri IskandarPerakMalaysia Faculty of Engineering and Natural Sciences Department of Mechanical EngineeringIstanbul Health and Technology UniversityIstanbulTurkey Department of Mechanical and Manufacturing Engineering Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia43600 UKM BangiSelangorMalaysia Faculty of Mechanical&Automotive Engineering Technology Universiti Malaysia Pahang26600 PekanPahangMalaysia

Benefits achieved by the biodegradable magnesium(Mg) and zinc(Zn) implants could be suppressed due to the invasion of infectious microbial, common bacteria, and fungi. Postoperative medications and the antibacterial properties of pure Mg and Zn are insufficient against biofilm and antibiotic-resistant bacteria, bringing osteomyelitis, necrosis, and even death. This study evaluates the antibacterial performance of biodegradable Mg and Zn alloys of different reinforcements, including silver(Ag), copper(Cu), lithium(Li), and gallium(Ga). Copper ions(Cu^(2+)) can eradicate biofilms and antibiotic-resistant bacteria by extracting electrons from the cellular structure. Silver ion(Ag^(+)) kills bacteria by creating bonds with the thiol group. Gallium ion(Ga^(3+)) inhibits ferric ion(Fe^(3+)) absorption, leading to nutrient deficiency and bacterial death. Nanoparticles and reactive oxygen species(ROS) can penetrate bacteria cell walls directly, develop bonds with receptors, and damage nucleotides. Antibacterial action depends on the alkali nature of metal ions and their degradation rate, which often causes cytotoxicity in living cells. Therefore, this review emphasizes the insight into degradation rate, antibacterial mechanism, and their consequent cytotoxicity and observes the correlation between antibacterial performance and oxidation number of metal ions.

关键词： Biodegradable materials Biomedical implants Antibacterial mechanism Cytotoxicity Reactive oxygen species

Isolation and Expression Analysis of FTZ-F1 Encoding Gene of Black Rock Fish (Sebastes schlegelii)

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Journal of Ocean University of China 2013年第1期12卷 183-189页

作者： Muhammad Shafi WANG Yanan ZHOU Xiaosu MA Liman faiz Muhammad QI Jie ZHANG Quanqi College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding of Ministry of Education of China Ocean University of China

Sex related FTZ-F1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, FTZ-F1 gene (FTZ-F1) was isolated from the testis of black rockfish (Sebastes schlegeli) by homology cloning. The full-length cDNA of S. schlegeli FTZ-F1 (ssFTZ-F1) contained a 232bp 5′UTR, a 1449bp ORF encoding FTZ-F1 (482 amino acid residules in length) with an estimated molecular weight of 5.4kD and a 105bp 3′UTR. Sequence, tissue distribution and phylogenic analysis showed that ssFTZ-F1 belonged to FTZ group, holding highly conserved regions including Ⅰ, Ⅱ and Ⅲ FTZ-F1 boxes and an AF-2 hexamer. Relatively high expression was observed at different larva stages. In juveniles (105 days old), the transcript of ssFTZ-F1 can be detected in all tissues and the abuncance of the gene transcript in testis, ovary, spleen and brain was higher than that in other tissues. In mature fish, the abundance of gene transcript was higher in testis, ovary, spleen and brain than that in liver (trace amount), and the gene was not transcribed in other tissues. The highest abundance of gene transcript was always observed in gonads of both juvenile and mature fish. In addition, the abundance of gene transcript in male tissues were higher than that in female tissue counterparts (P<0.05).

关键词： Sebastes schlegelii FTZ-F1 cloning expression