检索结果-南通市图书馆

Quantitative proteomics revealed extensive microenvironmental changes after stem cell transplantation in ischemic stroke

引用

Frontiers of Medicine 2022年第3期16卷 429-441页

作者： Yao Chen fahuan song Mengjiao Tu Shuang Wu Xiao He Hao Liu Caiyun Xu Kai Zhang Yuankai Zhu Rui Zhou Chentao Jin Ping Wang Hong Zhang Mei Tian Department of Nuclear Medicine and Medical PET Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Institute of Nuclear Medicine and Molecular Imaging Zhejiang UniversityHangzhou 310009China Key Laboratory of Medical Molecular Imaging of Zhejiang Province Hangzhou 310009China Shanxi Medical University Taiyuan 030001China Key Laboratory for Biomedical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China College of Biomedical Engineering and Instrument Science Zhejiang UniversityHangzhou 310027China Department of Radiology Zhejiang HospitalHangzhou 310030China Department of PET Center The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310006China

The local microenvironment is essential to stem cell-based therapy for ischemic stroke,and spatiotemporal changes of the microenvironment in the pathological process provide vital clues for understanding the therapeutic ***,relevant studies on microenvironmental changes were mainly confined in the acute phase of stroke,and long-term changes remain *** study aimed to investigate the microenvironmental changes in the subacute and chronic phases of ischemic stroke after stem cell ***,induced pluripotent stem cells(iPSCs)and neural stem cells(NSCs)were transplanted into the ischemic brain established by middle cerebral artery occlusion *** emission tomography imaging and neurological tests were applied to evaluate the metabolic and neurofunctional alterations of rats transplanted with stem *** proteomics was employed to investigate the protein expression profiles in iPSCs-transplanted brain in the subacute and chronic phases of *** with NSCs-transplanted rats,significantly increased glucose metabolism and neurofunctional scores were observed in iPSCs-transplanted *** proteomic data of iPSCs-transplanted rats identified a total of 39 differentially expressed proteins in the subacute and chronic phases,which are involved in various ischemic stroke-related biological processes,including neuronal survival,axonal remodeling,antioxidative stress,and mitochondrial function *** together,our study indicated that iPSCs have a positive therapeutic effect in ischemic stroke and emphasized the wide-ranging microenvironmental changes in the subacute and chronic phases.

关键词： ischemic stroke microenvironment induced pluripotent stem cells(iPSCs) positron emission tomography(PET) quantitative proteomics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Small-animal PET demonstrates brain metabolic change after using bevacizumab in a rat model of cerebral ischemic injury

引用

Neuroscience Bulletin 2014年第5期30卷 838-844页

作者： Ying Dong fahuan song Jianjuan Ma Xuexin He Said Amer Weizhong Gu Mei Tian Department of Oncology Department of Nuclear Medicine The Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang University Medical PET Center Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University Key Laboratory of Medical Molecular Imaging of Zhejiang Province Department of Zoology Faculty of ScienceKafr El Sheikh University Department of Pathology The Children's Hospital of Zhejiang University School of Medicine

ABSTRACT To evaluate the effect of bevacizumab on cerebral ischemia, we used 2-deoxy-2-18F-fluoro-D-glucose （18F-FDG） small-animal positron emission tomography （PET） in the middle cerebral artery occlusion （MCAO） rat model. After baseline neurologic function tests and PET studies, MCAO Sprague-Dawley rats received bevacizumab or normal saline （controls）. Weekly PET imaging and neurologic function tests showed that the 18F-FDG accumulation in the bevacizumab group was similar to that in the controls during the first 2 weeks, but lower than in controls at weeks 3 and 4. However, no difference was found in neurological scores between the groups. The number of von Willebrand factor-positive cells in the bevacizumab group was lower than that in controls. The expression of vascular endothelial growth factor was higher than in controls at week 4. These results suggested that bevacizumab does not influence functional recovery in this model of cerebral ischemia during a 4-week period, but inhibits vascular formation and metabolic recovery, which may be considered in cancer patients with a recent ischemic stroke.

关键词： cerebral ischemia bevacizumab positron emission tomography cancer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Resveratrol promotes the survival and neuronal differentiation of hypoxia-conditioned neuronal progenitor cells in rats with cerebral ischemia

引用

Frontiers of Medicine 2021年第3期15卷 472-485页

作者： Yao Yao Rui Zhou Rui Bai Jing Wang Mengjiao Tu Jingjing Shi Xiao He Jinyun Zhou Liu Feng Yuanxue Gao fahuan song Feng Lan Xingguo Liu Mei Tian Hong Zhang Department of Nuclear Medicine and PET-CT Center The Second Hospital of Zhejiang University School of MedicineHangzhou 310009China Beijing Laboratory for Cardiovascular Precision Medicine Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China Key Laboratory for Biomedical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Shanxi Medical University Taiyuan 030001China The College of Biomedical Engineering and Instrument Science of Zhejiang University Hangzhou 310027China

Hypoxia conditioning could increase the survival of transplanted neuronal progenitor cells(NPCs)in rats with cerebral ischemia but could also hinder neuronal differentiation partly by suppressing mitochondrial *** this work,the mitochondrial metabolism of hypoxia-conditioned NPCs(hcNPCs)was upregulated via the additional administration of resveratrol,an herbal compound,to resolve the limitation of hypoxia conditioning on neuronal *** was first applied during the in vitro neuronal differentiation of hcNPCs and concurrently promoted the differentiation,synaptogenesis,and functional development of neurons derived from hcNPCs and restored the mitochondrial ***,this herbal compound was used as an adjuvant during hcNPC transplantation in a photothrombotic stroke rat *** promoted neuronal differentiation and increased the long-term survival of transplanted hcNPCs.18-fluorine fluorodeoxyglucose positron emission tomography and rotarod test showed that resveratrol and hcNPC transplantation synergistically improved the neurological and metabolic recovery of stroke *** conclusion,resveratrol promoted the neuronal differentiation and therapeutic efficiency of hcNPCs in stroke rats via restoring mitochondrial *** work suggested a novel approach to promote the clinical translation of NPC transplantation therapy.

关键词： neuronal progenitor cells resveratrol cerebral ischemia neuronal differentiation mitochondrial metabolism positron emission tomography

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

脂肪肉瘤的18F-氟代脱氧葡萄糖PET- CT显像特征

引用

浙江大学学报（医学版） 2019年第2期48卷 193-199页

作者：付涧兰宋法寰程爱萍浙江省人民医院(杭州医学院附属人民医院)核医学科

目的:分析不同病理亚型脂肪肉瘤的18F-氟代脱氧葡萄糖(FDG) PET-CT显像特征,探讨PET-CT在脂肪肉瘤诊治中的应用价值。方法:回顾性分析13例经组织病理学检查证实的脂肪肉瘤患者的18F-FDG PET-CT显像资料。分析不同病理亚型脂肪肉瘤的PET... 详细信息

目的:分析不同病理亚型脂肪肉瘤的18F-氟代脱氧葡萄糖(FDG) PET-CT显像特征,探讨PET-CT在脂肪肉瘤诊治中的应用价值。方法:回顾性分析13例经组织病理学检查证实的脂肪肉瘤患者的18F-FDG PET-CT显像资料。分析不同病理亚型脂肪肉瘤的PET-CT表现,比较不同病理亚型脂肪肉瘤FDG代谢程度的差异及代谢的均匀性。结果:高分化型脂肪肉瘤以脂肪密度为主,均为单发,内有絮状间隔和(或)不规则形软组织团片影伴轻度代谢;黏液样脂肪肉瘤为囊性或囊实性肿块,单发或多发,代谢均匀或不均匀、轻中度增高;去分化型脂肪肉瘤为软组织密度影为主的混杂密度肿块,代谢不均匀,软组织成分代谢增高明显,常伴有大片片状坏死区;混合型脂肪肉瘤为高分化型与去分化型的混合类型,多发病灶,两种类型的影像表现均有。局部脂肪浸润12例,其中1例复发性去分化型脂肪肉瘤患者发生肺转移,所有患者均无淋巴结转移。FNCCLE分级为G1、G2和G3级患者脂肪肉瘤的最大标准摄取值(SUVmax)分别为3.00、5.67和10.33,差异有统计学意义(P<0.05),其中G1与G3、G2与G3级间差异均有统计学意义(均P<0.05)。结论:不同病理亚型脂肪肉瘤患者的PET-CT表现不同,术前PET-CT检查能明确肿瘤侵犯范围、有无转移情况,有利于初步判断脂肪肉瘤的病理亚型和评估组织学分级,有助于手术计划的制订,为临床决策提供更多信息。

关键词：脂肪肉瘤/病理学脂肪肉瘤/影像诊断氟脱氧葡萄糖F18/代谢体层摄影术,X线计算机正电子发射断层显像术/方法回顾性研究

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

大型超临界CO_2流体萃取装置的设计与开发

引用

世界科学技术-中医药现代化 2009年第6期11卷 892-896页

作者：孙太林宋泽耀葛发欢冉启军贵州航天乌江机电设备有限责任公司遵义563003 中药提取分离过程现代化国家工程研究中心广州510240

超临界CO_2流体萃取技术已广泛应用于天然产物的提取,成为中药现代化的关键技术之一,在中药新药的开发及中药生产中获得了成功的应用,并取得了良好的效果。本文主要报道了大型中药超临界CO_2流体萃取装置的设计与开发,着重讨论了超临界C... 详细信息

超临界CO_2流体萃取技术已广泛应用于天然产物的提取,成为中药现代化的关键技术之一,在中药新药的开发及中药生产中获得了成功的应用,并取得了良好的效果。本文主要报道了大型中药超临界CO_2流体萃取装置的设计与开发,着重讨论了超临界CO_2流体萃取装置中关键设备提取、分离容器的设计要点以及工业化装置开发应用过程中要解决的问题。设计与开发的大型超临界CO_2萃取装置已广泛应用于天然产物的提取及中药生产中。

关键词：超临界CO2流体装备设计开发

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：