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Transcriptome and carotenoid profiling of different varieties of Coffea arabica provides insights into fruit color formation

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Plant Diversity 2022年第3期44卷 322-334页

作者： faguang hu Xiaofei Bi Hongming Liu Xingfei Fu Yanan Li Yang Yang Xiaofang Zhang Ruirui Wu Guiping Li Yulan Lv Jiaxiong huang Xinping Luo Rui Shi Institute of Tropical and Subtropical Cash Crops Yunnan Academy of Agricultural SciencesChina Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of EducationSouthwest Forestry UniversityChina

The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured *** basis of genetic variation in coffee fruit quality could be explained by studying color formation during fruit *** profiling was conducted on matured fruits of four *** varieties(orange colored fruits(ORF);purple colored fruits(PF);red colored fruits(RF)and yellow colored fruits(YF))to identify key color-regulating genes,biosynthesis pathways and transcription factors implicated in fruit color formation.A total of 39,938 genes were identified in the transcriptomes of the four *** *** all,2745,781 and 1224 differentially expressed genes(DEGs)were detected in YF_vs_PF,YF_vs_RF and YF_vs_ORF,respectively,with 1732 DEGs conserved among the three pairwise *** annotation of the DEGs led to the detection of 28 and 82 key genes involved in the biosynthesis of carotenoids and anthocyanins,*** transcription factors bHLH,MYB,NAC,MADS,and WRKY implicated in fruit color regulation were *** high expression levels of gene-LOC113688784(PSY),gene-LOC113730013(b-CHY),gene-LOC113728842(CCD7),gene-LOC113689681(NCED)and gene-LOC113729473(ABA2)in YF may have accounted for the yellow *** differential expression of several anthocyanin and carotenoid-specific genes in the fruits substantially account for the purple(PF),red(RF),and orange(ORF)*** study provides important insights into fruit color formation and variations in *** and will help to develop coffee varieties with specific color and quality traits.

关键词： Coffee Carotenoids Anthocyanins Transcription factors Functional enrichment

Damage and Control Suggestions for the Emerging Disease Coffee Root Rot

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Plant Diseases and Pests 2024年第4期15卷 13-16页

作者： Xingfei FU Guiping LI faguang hu Institute of Tropical and Subtropical Cash Crops Yunnan Academy of Agricultural SciencesBaoshan 678000China

[Objectives]The paper was to elucidate the symptoms,morphological characteristics,etiological factors,and current extent of damage associated with coffee root rot disease.[Methods]The symptoms,morphological characteristics,and etiological factors associated with coffee root rot disease were systematically observed,described,and *** assessment of damage was carried out using a specialized investigative methodology.[Results]The application of bottom fertilizer containing Fusarium incarnatum facilitated the pathogen's entry through root wounds during transplantation,resulting in the development of water-soaked depression lesions in the affected *** infection significantly reduced the number of lateral roots in coffee plants,leading to symptoms such as wilting,withering,and ultimately,the death of the aboveground *** exhibited three distinct types of spore morphology:macroconidia,which were sickle-shaped;mesoconidia,which were spindle-shaped;and microconidia,which were *** incidence rate of the disease in the affected region reached 100%,with a disease index exceeding 91,indicating severe damage.[Conclusions]This study serves as a valuable reference for the prevention and management of the emerging disease known as coffee root rot.

关键词： Coffea arabica Root rot Fusarium incarnatum Prevention and control New diseases

Identification of Hyperparasitic Fungal Species Associated with Coffee Leaf Rust

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Plant Diseases and Pests 2024年第5期15卷 5-7页

作者： Xingfei FU Jianxin CHEN Guiping LI Haohao YU Yaqi LI Xiaofei BI faguang hu Institute of Tropical and Subtropical Cash Crops Yunnan Academy of Agricultural SciencesBaoshan 678000China Southwest Forestry University Kunming 650224China

[Objectives]The paper was to elucidate the specific hyperparasitic fungal species that are associated with coffee leaf rust.[Methods]Tissue isolation,sample humidification,and three-point inoculation techniques were employed to isolate,culture,and purify the hyperparasitic fungi responsible for coffee leaf *** purified strains were identified using traditional morphological techniques and molecular biology methods.[Results]Four strains were isolated,specifically BS21(Cladosporium cladosporioides),BS34(***),BS62(***),and BS75(***).[Conclusions]The findings of this research will contribute novel insights into the biological control of coffee leaf rust.

关键词： Leaf rust Hyperparasitic fungi Isolation Identification

Integrated Prevention and Control Technology for Coffee Leaf Rust in Yunnan Province

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Asian Agricultural Research 2024年第11期16卷 25-28页

作者： Xingfei FU Guiping LI faguang hu Institute of Tropical and Subtropical Cash Crops Yunnan Academy of Agricultural SciencesBaoshan 678000China

This paper investigates the damage symptoms and occurrence regularity related to coffee leaf rust,and proposes a comprehensive prevention and control strategy grounded in the principle of prioritizing prevention and implementing integrated prevention and *** strategy encompasses the cultivation of rust-resistant varieties,the implementation of agricultural practices,the application of chemical interventions,the utilization of hyperparasitic fungi,and the protection and utilization of natural *** paper further outlines the necessary requirements for effective prevention and control,emphasizing the importance of enhancing responsibility implementation,fostering systematic prevention and control measures,enhancing guidance services,and increasing publicity and *** aim is to offer technical guidance for the integrated prevention and control of coffee leaf rust in Yunnan Province.

关键词： Coffee leaf rust disease Damage symptoms Occurrence regularity Integrated prevention and control Yunnan Province

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云南咖啡产业高质量发展对策初探

热带农业科技 2022年第3期45卷 21-29页

作者：黄家雄李维锐夏兵胡发广云南省农业科学院热带亚热带经济作物研究所云南保山678000 云南省热带作物学会昆明650041 云南省农业农村厅昆明650000

分析了云南咖啡产业发展基本情况,总结云南咖啡产业发展成效和制约云南咖啡产业发展的主要因素,初步探讨了加快推动云南咖啡产业高质量发展的对策。

关键词：咖啡高质量发展对策云南

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超声辅助低共熔溶剂制备咖啡果皮可溶性膳食纤维

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热带作物学报 2024年第1期45卷 176-186页

作者：邵芳芳胡发广董文江毕晓菲陈罡陈舰飞宁夏大学食品与葡萄酒学院宁夏银川750021 中国热带农业科学院香料饮料研究所海南万宁571533 云南省农业科学院热带亚热带经济作物研究所云南保山678000 普洱富民农业装备有限公司云南普洱665000 海南省特色热带作物适宜性加工与品质控制重点实验室海南万宁571533

为进一步探索制备咖啡果皮中可溶性膳食纤维的绿色新方法。本研究建立了一种省时、高效、环保的超声辅助低共熔溶剂(UAE-DESs)制备咖啡果皮可溶性膳食纤维(SDF)的方法,并通过单因素试验和响应面试验优化了其工艺参数。采用加热法制备了... 详细信息

为进一步探索制备咖啡果皮中可溶性膳食纤维的绿色新方法。本研究建立了一种省时、高效、环保的超声辅助低共熔溶剂(UAE-DESs)制备咖啡果皮可溶性膳食纤维(SDF)的方法,并通过单因素试验和响应面试验优化了其工艺参数。采用加热法制备了8种氯化胆碱基的DESs作为提取溶剂,并与传统水提法作对比。结果表明:氯化胆碱与尿素摩尔比为1∶2的DES体系制备的咖啡果皮SDF得率最高,为10.10%±0.11%,显著高于传统水提法(6.50%±0.22%)。通过单因素实验,探讨了DES组成(摩尔比、含水量、液固比)和超声波处理(超声功率、超声时间)对咖啡果皮SDF得率的影响。在此基础上,采用响应面法(RSM)结合Box-Behnken设计(BBD)对SDF得率进行优化,考察DES含水量、液固比、超声功率和超声时间对SDF得率的影响。根据RSM的优化结果,确定最佳制备条件为:DES含水量为39.91%,液固比为32.37 mL/g,超声功率为305.20 W,超声时间为33.80 min,在此条件下,咖啡果皮SDF得率最大,为10.74%±0.14%,与其预测值10.80%接近。因此,DES作为一种高效、无污染的绿色溶剂,可用于咖啡果皮SDF的制备。本研究结果将为咖啡果皮可溶性膳食纤维的制备提供新思路和理论参考,并在咖啡副产物的增值利用、生产新型高附加值产品以及开发天然功能性食品等方面具有潜在的应用前景。

关键词：咖啡果皮低共熔溶剂可溶性膳食纤维响应面法

不同壁材对绿咖啡油微胶囊微观结构及其热稳定性的影响

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热带作物学报 2023年第3期44卷 647-660页

作者：慕静怡胡发广张珍珍董文江李亚男毕晓菲胡荣锁陈小爱新疆农业大学食品科学与药学学院新疆乌鲁木齐830053 中国热带农业科学院香料饮料研究所海南万宁571533 云南省农业科学院热带亚热带经济作物研究所云南保山678000 海南省特色热带作物适宜性加工与品质控制重点实验室海南万宁571533

分别以阿拉伯胶(gum arabic,GA)与大豆分离蛋白(soybean protein isolate,SPI)、乳清分离蛋白(whey protein isolate,WPI)和酪蛋白酸钠(sodium caseinate,SC)作为壁材,通过复合凝聚法制备3种绿咖啡油(green coffee oil,GCO)微胶囊。结合不同表征技术手段,对壁材的浊度、红外光谱特征、表观形貌以及荧光共定位进行分析;比较3种绿咖啡油微胶囊的包封效率、形貌、粒径、结构表征及热稳定性。结果表明,阿拉伯胶与不同蛋白之间通过静电相互作用形成复聚物,可作为包埋绿咖啡油的新型壁材;红外光谱分析表明,3种壁材均能较好地包埋绿咖啡油,包埋率从大到小依次为SPIGA-GCO(69.26%)>SCGA-GCO(58.69%)>WPIGA-GCO(52.57%);绿咖啡油微胶囊的微观结构表面平坦,无明显裂纹,其中SPIGA-GCO中油滴分布较为均匀;差示扫描热量法分析表明,微胶囊热变性温度较高(103.93~108.33℃),具有良好的热稳定性。研究结果表明,SPIGA-GCO微胶囊性能明显优于其他2种壁材制备的微胶囊产品。本研究为开发稳定有效的绿咖啡油微胶囊产品提供技术支撑,也为绿咖啡油的高值化利用提供理论依据。

关键词：绿咖啡油微胶囊复合凝聚法结构表征热稳定性

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小粒咖啡不同苗龄沙床苗对育成容器苗质量的影响

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热带农业科技 2017年第4期40卷 17-19页

作者：胡发广毕晓菲黄家雄李亚男云南省农业科学院热带亚热带经济作物研究所云南保山678000

为探讨小粒咖啡沙床苗质量对容器苗质量的影响,以Catimor7963不同苗龄沙床苗为试材进行移栽容器育苗试验,120 d后观测株高、基茎、叶片数、主根长、侧根数及苗木整齐度。结果表明,在参试苗龄范围内,随着沙床苗苗龄的增加,育成的容器苗... 详细信息

为探讨小粒咖啡沙床苗质量对容器苗质量的影响,以Catimor7963不同苗龄沙床苗为试材进行移栽容器育苗试验,120 d后观测株高、基茎、叶片数、主根长、侧根数及苗木整齐度。结果表明,在参试苗龄范围内,随着沙床苗苗龄的增加,育成的容器苗质量也逐步提升,从对地上部分生长量和植株整齐度的影响来看,两叶一心苗处理的影响极显著和显著地优于其它苗龄处理。

关键词：小粒咖啡沙床苗容器苗苗木质量

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小粒咖啡初加工方法概述

农产品加工（下） 2017年第3期 66-68,70页

作者：胡发广毕晓菲黄家雄李亚男云南省农业科学院热带亚热带经济作物研究所云南保山678000

咖啡初加工是影响咖啡产品品质的核心因素,不同方法加工的咖啡产品又表现出不同的特质。通过概述目前11种咖啡初加工方法、技术流程及优缺点,以期为云南咖啡初加工技术改进提升提供参考。

关键词：咖啡初加工小粒咖啡加工方法