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In silico docking analysis of beta-defensin 20 against cation channel sperm-associated protein 1–4 to predict its role in the sperm maturation

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Asian Journal of Andrology 2023年第4期25卷 528-532页

作者： Luluk Yunaini Linda Erlina fadilah fadilah Dwi Ari Pujianto Doctoral Program for Biomedical Sciences Faculty of MedicineUniversitas IndonesiaJakarta 10430Indonesia Department of Medical Biology Faculty of MedicineUniversitas IndonesiaJakarta 10430Indonesia Department of Medical Chemistry Faculty of MedicineUniversitas IndonesiaJakarta 10430Indonesia Bioinformatics Core Facilities Indonesia Medical Education and Research Institute(IMERI)Faculty of MedicineUniversitas IndonesiaJakarta 10430Indonesia

Beta-defensin 20(DEFB20)is widely expressed in the epididymis with gene features involved in epididymal sperm maturation.However,the action mechanism and function of DEFB20 in sperm maturation are still unclear.One of the important roles of beta-defensin is the ion channel activity.The cation channel sperm-associated protein(CatSper)alpha is an ion channel protein found on the sperm surface.This study aimed to investigate the interaction between DEFB20 and CatSper1–4 protein in relation to the sperm maturation process.Protein sequences were obtained from the National Center for Biotechnology Information(NCBI).Protein modeling and validation were carried out by using the Robetta modeling server and the Ramachandran plot method.Rosetta web server was used for the docking analysis.The results revealed a natural interaction between DEFB20 and CatSper1–4.The interaction occurred at the cation channel(close to the casein kinase II),ion transport protein,and kinase c phosphorylation of the CatSper1–4 active site.The DEFB20 region interacting with CatSper2–4 was the beta-defensin domain,while with CatSper1 was the non-beta-defensin domain.Based on the analysis,DEFB20 may interact with CatSperαsubunits,particularly CatsSper1,to affect ion channel activity during sperm maturation.

关键词： CatSper DEFB20 docking sperm maturation

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Case series in Indonesia:B.1.617.2(delta)variant of SARS-CoV-2 infection after a second dose of vaccine

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World Journal of Clinical Cases 2022年第36期10卷 13216-13226页

作者： Anis Karuniawati Ari F Syam Armand Achmadsyah Fera Ibrahim Yulia Rosa Pratiwi Sudarmono fadilah fadilah Menaldi Rasmin Department of Clinical Microbiology Faculty of MedicineUniversitas Indonesia/Cipto Mangunkusumo Jakarta IndonesiaJakarta 10430DKI JayaIndonesia Division of Gastroenterology Department of Internal MedicineFaculty of MedicineUniversitas Indonesia/Cipto Mangunkusumo Jakarta IndonesiaJakarta PUsat 10430DKI JayaIndonesia Faculty of Medicine Universits IndonesiaJakarta Pusat 10430DKI JayaIndonesia Department of Medical Chemistry Faculty of MedicineUniversitas IndonesiaJakarta IndonesiaFaculty of MedicineUniversitas IndonesiaJakarta 10430DKI JayaIndonesia Department of Pulmonology and Respiratory Medicine Faculty of MedicineUniversitas Indonesia/Persahabatan HospitalJakarta IndonesiaJakarta Pusat 10430DKI JayaIndonesia

BACKGROUND The B.1.617.2(delta)variant of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was first discovered in Maharashtra in late 2020 and has rapidly expanded across India and worldwide.It took only 2 mo for this variant to spread in Indonesia,making the country the new epicenter of the delta variant as of July 2021.Despite efforts made by accelerating massive rollouts of current vaccines to protect against infection,cases of fully-vaccinated people infected with the delta variant have been reported.AIM To describe the demographic statistics and clinical presentation of the delta variant infection after the second dose of vaccine in Indonesia.METHODS A retrospective,single-centre case series of the general consecutive population that worked or studied at Faculty of Medicine,Universitas Indonesia with confirmed Delta Variant Infection after a second dose of vaccine from 24 June and 25 June 2021.Cases were collected retrospectively based on a combination of author recall,reverse transcription-polymerase chain reaction(RT-PCR),and whole genome sequencing results from the Clinical Microbiology Laboratory,Faculty of Medicine,Universitas Indonesia.RESULTS Between 24 June and 25 June 2021,15 subjects were confirmed with the B.1.617.2(delta)variant infection after a second dose of the vaccine.Fourteen subjects were vaccinated with CoronaVac(Sinovac)and one subject with ChAdOx1 nCoV-19(Oxford-AstraZeneca).All of the subjects remained in home isolation,with fever being the most common symptom at the onset of illness(n=10,66.67%).The mean duration of symptoms was 7.73 d(±5.444).The mean time that elapsed from the first positive swab to a negative RT-PCR test for SARS-CoV-2 was 17.93 d(±6.3464).The median time that elapsed from the second dose of vaccine to the first positive swab was 87 d(interquartile range:86-128).CONCLUSION Although this case shows that after two doses of vaccine,subjects are still susceptible to the delta variant infection,currently available vaccines remain the most effective protection

关键词： COVID-19/SARS-CoV-2 infection B.1.617.2(delta)variant Fully vaccinated Case series

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Influence of urban air pollution on the population in the Klang Valley,Malaysia:a spatial approach

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Ecological Processes 2022年第1期11卷 126-141页

作者： Siti Haslina Mohd Shafie Mastura Mahmud Suzani Mohamad Nor Lita fadilah Rameli Ramdzani Abdullah Ahmad Fariz Mohamed Centre for Research in Development Social and Environment(SEEDS)Faculty of Social Sciences and HumanitiesUniversiti Kebangsaan MalaysiaBangiSelangorMalaysia Department of Environment Faculty of Forestry and Envi-ronmentUniversiti Putra MalaysiaSerdangSelangorMalaysia Institute for Environment and Development(LESTARI) Universiti Kebangsaan MalaysiaBangiSelangorMalaysia.

Background:Urban air pollution resulting from economic growth as well as urbanization impacts the health of inhabitants.This study aims to examine the spatial distribution of particulate matter(PM_(10))and the risk of exposure for the population in the Klang Valley,an urban conglomeration centred in Kuala Lumpur,Malaysia.Methods:The inverse distance weighted(IDW)interpolation technique in the ArcGIS platform was used to map the air quality from five air monitoring stations.Spatial statistics of the Global Moran’s I Spatial Autocorrelation and Optimized Hot Spot Analysis were used to estimate the spatial clustering of PM_(10).The population-weighted exposure level(PWEL)technique was used to calculate the population density susceptible to PM_(10)and the AirQ+model was applied to estimate the proportion of the adult population at risk of chronic bronchitis due to long-term exposure to PM_(10)concentrations in the Klang Valley.Results:The highest annual mean PM_(10)concentrations at the Klang station ranged between 80 and 100μg/m^(3)from 2000 to 2009 and exceeded the New Malaysia Ambient Air Quality Standard(NMAAQS)and the World Health Organi-zation Air Quality Guidelines(WHOAQG)levels of 40 and 20μg/m^(3),respectively.The Moran’s I results indicated that the statistically significant clusters of high PM_(10)in Klang present a health risk to the local community.The PWEL results showed that levels of PM_(10)in Petaling Jaya exceeded the WHOAQG limits,potentially posing a health risk to the high-density population.The AirQ+model found Klang to be a significantly high-risk area for chronic bronchitis among the adult population with 804,240 people(with a relative risk of 1.85)in 2009.Conclusion:These high-risk values are due to rapid urbanization and high motor vehicle usage in the Klang Valley and therefore the impact of PM_(10)on the population needs to be reduced.This situation should be taken seriously by the Department of Environment and Kuala Lumpur City Hall in addressing air pollution issues through stricter rule

关键词： Population Urban air pollution Spatial distribution PM_(10) Adult chronic bronchitis risk

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Kinetic chain disruption contributes to shoulder and elbow injuries

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Sports Medicine and Health Science 2021年第4期3卷 252-253页

作者： Qais Gasibat Tengku fadilah Tengku Kamalden Department of Sport Studies University Putra Malaysia(UPM)Kuala LumpurMalaysia

The kinetic chain or link theory provides the basis for understanding and evaluating human movement patterns as well as the justification for using exercise training and recovery services that emphasise the whole body,despite the damage to a specific joint or anatomical structure.The motion of one segment affects all proximal and distal segments to the first segment.The overhead throwing motion is produced and regulated by a sequential position and motion of the body,involving sequential activation.

关键词： Shoulder injuries Elbow injuries Kinetic chain Range of motion

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Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 813-819页

作者： Izatus Shima TAIB Siti Balkis BUDIN Seri Maseran SITI NOR AIN Jamaludin MOHAMED Santhana Raj LOUIS Srijit DAS Sulaiman SALLEHUDIN Nor fadilah RAJAB Othman HIDAYATULFATHI Department of Biomedical Sciences Faculty of Allied Health SciencesUniversiti Kebangsaan MalaysiaJalan Raja Muda Abdul Aziz50300 Kuala LumpurMalaysia Unit of Electron Microscope Institute for Medical ResearchJalan Pahang50588 Kuala LumpurMalaysia Department of Anatomy Universiti Kebangsaan MalaysiaJalan Raja Muda Abdul Aziz50300 Kuala LumpurMalaysia

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity,chemopreventative and insecticidal properties. In this study,the toxic effects of L. elliptica essential oil against Sprague-Dawley rat’s red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125,250,and 500 mg/(kg body weight),respectively,and the control group received distilled water. Full blood count,RBC osmotic fragility,RBC morphological changes,and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb),mean cell hemoglobin concentration (MCHC),mean cell volume (MCV),and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.

关键词： SD大鼠毒性作用木姜子红细胞精油椭圆

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The Effect of Reflective Learning on Childbirth Care Competence of Midwifery Students

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Open Journal of Nursing 2019年第3期9卷 209-220页

作者： Lola Noviani fadilah Farid Husin Juntika Nurihsan Tria Giri Ramdani Politeknik Kesehatan Kementerian Kesehatan Bandung Bandung Indonesia Universitas Padjadjaran Bandung Indonesia Universitas Pendidikan Indonesia Bandung Indonesia Dinas Kesehatan Provinsi Jawa Barat Bandung Indonesia

Background: The skills of midwives in childbirth care competence are still inadequate. This problem is influenced by limitation experience of midwifery students of childbirth care in the field of practice area. That condition related to the proportion of midwifery students and case of childbirth woman was an imbalance. Midwifery students must prepare this competence in an education with childbirth care experience from practice in the laboratory and in the field of practice. Reflective learning is an appropriate learning method that uses experience as the basis of learning to improve childbirth care competence of midwifery students. Objective: This study is to analyze the effect of reflective learning implementation in childbirth care practice to improve students’ competence. Method: This research uses quasi-experimental design with non-equivalent control group design. The respondents were midwifery students in the fourth semester who had experienced the theory of childbirth care. There were totally 64 samples, 34 samples for treatment group and 30 samples for control group. Data were analyzed by using unpaired t test, Mann Whitney U and linear regression. Results: The respondents were homogeny in motivation and grade point of average (GPA). The improvement of childbirth care competency is influenced by treatment of 19.064 with R2 = 57.7%. Conclusion: The implementation of reflective learning in childbirth care practice affected the improvement of students’ competence.

关键词： Reflective Learning Competence of Childbirth Care Midwifery Students

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蒌叶提取物增强5-氟尿嘧啶对结肠癌细胞HT29和HCT116的生长抑制作用(英文)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第8期15卷 692-700页

作者： Pek Leng NG Nor fadilah RAJAB Sue Mian THEN Yasmin Anum MOHD YUSOF Wan Zurinah WAN NGAH Kar Yong PIN Mee Lee LOOI Department of Biomedical Sciences Faculty of Allied Health Universiti Kebangsaan Malaysia (UKM) Jalan Raja Muda Abdul Aziz50300 Kuala Lumpur Malaysia UKM Medical Molecular Biology Institute (UMBI) Universiti Kebangsaan Malaysia (UKM) Jalan Yaacob Latiff Bandar Tun Razak Cheras56000 Kuala Lumpur Malaysia School of Biomedical Science University of Nottingham Malaysia Campus43500 Semenyih Selangor Malaysia Department of Biochemistry Faculty of Medicine Universiti Kebangsaan Malaysia (UKM) Jalan Raja Muda Abdul Aziz50300 Kuala Lumpur Malaysia Forest Research Institute Malaysia (FRIM) 52109 Kuala Lumpur Kepong Malaysia School of Biosciences Taylor’s University Lakeside Campus47500 Subang Jaya Selangor Malaysia

研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测H... 详细信息

研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测HT29和HCT116细胞的凋亡情况,高效液相色谱法排除PB和5-FU间任何可能的相互化学作用。重要结论:联合PB,低剂量5-FU可以在短时间内起到细胞毒作用,而单独应用PB或5-FU治疗较联合治疗可以诱导更多细胞发生凋亡。进一步采用等效线图法分析显示PB和5-FU的联合作用在抑制结肠癌细胞HT29和HCT116的生长中分别体现出协同和拮抗作用。因此可以认为在HT29细胞中,PB使得较低剂量5-FU发挥最大抑制结肠癌细胞生长效果,然而在HCT116细胞中,PB没有显著降低5-FU的药物浓度,说明PB和5-FU的相互作用不仅仅体现在诱导细胞凋亡方面。

关键词：胡椒科蒌叶 5-氟尿嘧啶等效线图法草药-药物相互作用

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Design and Molecular Docking Study of Antimycin A3Analogues as Inhibitors of Anti-Apoptotic Bcl-2 of Breast Cancer

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Open Journal of Medicinal Chemistry 2014年第3期4卷 79-86页

作者： Ade Arsianti fadilah Kusmardi Hiroki Tanimoto Tsumoru Morimoto Kiyomi Kakiuchi Department of Medical Chemistry Faculty of Medicine University of Indonesia Jakarta Indonesia Department of Anatomical Pathology Faculty of Medicine University of Indonesia JakartaIndonesia Graduate School of Materials Science Nara Institute of Science and Technology (NAIST) Nara Japan

In this paper, we report the design and moleculardocking study of analogues of antimycin A3 as inhibitors of anti-apoptotic Bcl-2 of breast cancer. Twenty designed compounds and the original antimycin A3 were docked based on their interaction with breast tumor receptor binding target Bcl-2. The docking resulted in the five top-ranked compounds, namely, compounds 11, 14, 15, 16, and 20, which have a lower G binding energy, better affinity and stronger hydrogen bonding interactions to the active site of Bcl-2 than antimycin A3. Among those five top-ranked compounds, analogue compounds 11 and 14, which have an 18-membered tetralactone core and 18-membered tetraol core, respectively, exhibited the strongest hydrogen bond interaction, formed high stability conformation, and demonstrated the greatest inhibitory activity on the catalytic site of Bcl-2.

关键词： Design Docking Antimycin A3 Analogue Bcl-2 Breast Cancer

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Geological Studies to Support the Tourism Site: A Case Study in the RafflesiaTrail, Near Kampung Jedip, Lojing Highlands, Kelantan, Malaysia

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International Journal of Geosciences 2014年第8期5卷 835-851页

作者： Dony Adriansyah Nazaruddin Nur Syazwani Md. fadilah Zurfarahin Zulkarnain Sharifah Aisyah Syed Omar Mohamad Khidzir Mohd Ibrahim Geoscience Programme Faculty of Earth Sciences Universiti Malaysia Kelantan UMK Jeli Campus Jeli Malaysia Laboratory of Geology and Soils Science Faculty of Earth Sciences Universiti Malaysia Kelantan UMK Jeli Campus Jeli Kelantan Malaysia Natural Resources Programme Faculty of Earth Sciences Universiti Malaysia Kelantan UMK Jeli Campus Jeli Malaysia

Some geological studies were performed in the Rafflesia Trail, near Kampung Jedip, in Lojing Highlands, Kelantan. This area is famous for its Rafflesia and has become one of the tourism attractions in Kelantan. However, the recent development of agricultural activities has significant effects on the area. The objective of these studies is to give substantial geological information including geomorphology, lithology, structural features, geohazard potentials, and water quality analysis in the study area. Desk study was conducted by reviewing some literatures related to the topic and the study area. Field work was organized during a scientific expedition in January 2014, to collect data, samples, and photographs. Geomorphologically, the study area is a mountainous area which mostly consists of mountain ridges and mountain valleys. Some fluvial features occur in the study area, such as waterfalls, cascades, rapids, runs, pools, potholes, lateral bars, and point bars. This area is composed of granitic rocks, mostly granite porphyry, and covered by superficial deposits of Quaternary age. The geological structure that is commonly found in the study area is joints, where they divide the rock body into large, roughly angular blocks, which is called as brecciation. The potential geohazard in the study area is landslides, where some of them are composed of soil only and others are mixtures of rock and soil. The water quality analysis has managed to identify that the stream water in this area generally has the quality of Class III (according to INWQS for Malaysia). These studies recommend that this area should be supported as a sustainable tourism site in Lojing Highlands.

关键词： Geological Studies Sustainable Tourism Site The Rafflesia Trail Lojing Highlands Kelantan

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Genotoxic Potential of Shrimp Pastes (Belacan) Extracts Using UmuTest

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Food and Nutrition Sciences 2012年第4期3卷 522-525页

作者： Ahmad Rohi Ghazali Yvonne Sipain Nor fadilah Rajab Siew Ee Ling Norain Ramli Rozaini Abdullah Zaliha Harun Firdaus Kamarulzaman Biomedical Science Program Faculty of Health Sciences School of Diagnostic and Applied Health Sciences Universiti Kebangsaan Malaysia Kuala Lumpur Malaysia Environmental Health Program Faculty of Health Sciences School of Diagnostic and Applied Health Sciences Universiti Kebangsaan Malaysia Kuala Lumpur Malaysia.

This study was conducted to observe the genotoxic effects of aqueous, methanol, hexane and dichloromethane extracts of “belacan” (shrimp paste) taken from three local districts in Melaka, Malaysia (Kelemak, Batang Tiga & Pantai Puteri). The umu test which was used as the screening test was conducted with and without the presence of metabolic activation system. Without the presence of metabolic activation system, aqueous extracts from Kelemak showed mutagenicity activity at 5 mg/ml with IR (Induction Rate) = 1.52 ± 0.57 and the methanol extracts showed mutagenic activities at 0.625 mg/ml and 5 mg/ml, which the IR was the highest at 5 mg/ml (2.08 ± 0.09). On the other hand, samples from Batang Tiga, Melaka showed mutagenic effects at all five concentrations for the dichloromethane extract, with IR = 2.09 ± 0.64 as the highest value at 1.25 mg/ml. Methanol extracts also showed positive results at 1.25 mg/ml and 2.5 mg/ml with IR = 1.70 ± 0.33 and IR = 2.12 ± 0.51 respectively, and aqueous extract at 0.625 mg/ml with IR = 1.54 ± 0.48 and 5 mg/ml with IR = 1.74 ± 0.50. There was a significant difference of the mean values of IR between the four different types of “belacan” extracts from Batang Tiga (p < 0.05). All four “belacan” extracts from Pantai Puteri, Melaka did not show any mutagenic effect. With the presence of metabolic activation system, there was no mutagenic effect observed in all four extracts from the three districts. Further study to analyze the contents in the food samples should be done in the future to determine the possible contents in the food samples that might be responsible for the mutagenic activities.

关键词： Shrimp Pastes Mutagenicity Umu Test Melaka Genotoxicity

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