检索结果-南通市图书馆

Differentiated cells derived from fetal neural stem cells improve motor deficits in a rat model of Parkinson's disease

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Neuroscience and Clinics 2015年第2期1卷 75-85页

作者： Wei Wang Hao Song Aifang Shen Chao Chen Yanming Liu Yabing Dong fabin han Centre for Stem Cells and Regenerative Medicine Liaocheng People’s Hospital/The Affiliated Liaocheng Hospital Taishan Medical University

Objective: Parkinson's disease(PD), which is one of the most common neuro‐degenerative disorders, is characterized by the loss of dopamine(DA) neurons in the substantia nigra in the midbrain. Experimental and clinical studies have shown that fetal neural stem cells(NSCs) have therapeutic effects in neurological disorders. The aim of this study was to examine whether cells that were differentiated from NSCs had therapeutic effects in a rat model of PD. Methods: NSCs were isolated from 14‐week‐old embryos and induced to differentiate into neurons, DA neurons, and glial cells, and these cells were characterized by their expression of the following markers: βⅢ‐tubulin and microtubule‐associated protein 2(neurons), tyrosine hydroxylase(DA neurons), and glial fibrillary acidic protein(glial cells). After a 6‐hydroxydopamine(6‐OHDA)‐lesioned rat model of PD was generated, the differentiated cells were transplanted into the striata of the 6‐OHDA‐lesioned PD rats. Results: The motor behaviors of the PD rats were assessed by the number of apomorphine‐induced rotation turns. The results showed that the NSCs differentiated in vitro into neurons and DA neurons with high efficiencies. After transplantation into the striata of the PD rats, the differentiated cells significantly improved the motor deficits of the transplanted PD rats compared to those of the control nontransplanted PD rats by decreasing the apomorphine‐induced turn cycles as early as 4 weeks after transplantation. Immunofluorescence analyses showed that the differentiated DA neurons survived more than 16 weeks. Conclusions: Our results showed that cells that were differentiated from NSCs had therapeutic effects in a rat PD model, which suggests that differentiated cells may be an effective treatment for patients with PD.

关键词： fetal neural stem cells Parkinson’s disease transplantation differentiation dopamine neuron

The application of patient-derived induced pluripotent stem cells for modeling and treatment of Alzheimer’s disease

在线全文

学校读者我要写书评

暂无评论

Brain Science Advances 2019年第1期5卷 21-40页

作者： fabin han Chuanguo Liu Jin Huang Juanli Chen Chuanfei Wei Xiwen Geng Yanming Liu Dong han Mengpeng Li The Institute for Tissue Engineering and Regenerative Medicine Liaocheng University/Liaocheng People’s HospitalShandong 252000China The Translational Research Lab for Stem Cells and Traditional Chinese Medicine Shandong University of Traditional Chinese MedicineJinanShandong 250355China Key Laboratory of Tissue Repair and Regeneration of PLA and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationThe Fourth Medical Centre of PLA General HospitalBeijing 100048China Shandong Molecular Diagnostics&Cell Therapeutic Biotechnology Corporation Ji’nanShandong 250001China

Alzheimer’s disease(AD)is the most prevalent age-related neurodegenerative disease which is mainly caused by aggregated protein plaques in degenerating neurons of the *** aggregated protein plaques are mainly consisting of amyloidβ(Aβ)fibrils and neurofibrillary tangles(NFTs)of phosphorylated tau *** though the transgenic murine models can recapitulate some of the AD phenotypes,they are not the human cell models of *** breakthrough in somatic cell reprogramming made it available to use induced pluripotent stem cells(i PSCs)for patientspecific disease modeling and autologous transplantation *** i PSCs provide alternative ways to obtain specific human brain cells of AD patients to study the molecular mechanisms and therapeutic approaches for familial and sporadic forms of *** differentiation into neuronal cells,i PSCs have enabled the investigation of the complex aetiology and timescale over which AD develops in human ***,we first go over the pathological process of and transgenic models of *** we discuss the application of i PSC for disease model and cell *** last the challenges and future applications of i PSCs for AD will be summarized to propose cell-based approaches for the treatment of this devastating disorder.

关键词： Alzheimer’s disease(AD) induced pluripotent stem cell(iPSC) amyloid-beta plaque(Aβplaque) neural stem cell(NSC) transplantation mutation

Research advances on antioxidation,neuroprotection,and molecular mechanisms of Lycium barbarum polysaccharides

在线全文

学校读者我要写书评

暂无评论

Brain Science Advances 2021年第4期7卷 207-219页

作者： Cheng Wang Liangxing Zhou Mei Mo Xianglin Kong Zhengbin Chai Lei Deng Junli Zhang Kuan Cao Chuanfei Wei Li Xu Juanli Chen fabin han The Innovation Institute for Traditional Chinese Medicine Shandong University of Traditional Chinese MedicineJinan 250355ShandongChina Experimental Centre Shandong University of Traditional Chinese MedicineJinan 250355ShandongChina The Institute for Tissue Engineering and Regenerative Medicine Stem Cell and Regenerative Medicine LaboratoryLiaocheng University/Liaocheng People’s HospitalLiaocheng 252000ShandongChina

Lycium barbarum polysaccharides(LBPs)are the major polysaccharides extracted from ***,which is used in traditional Chinese medicine(TCM)for treating *** have shown that LBPs have important biological activities,such as antioxidation,anti-aging,neuroprotection,immune *** inhibit oxidative stress,improve neurodegeneration and stroke-induced neural injury,increase proliferation and differentiation of neural stem cell,and promote neural *** we have reviewed latest advances in the biomedical activities of LBPs and improved methods for the isolation,extraction,and purification of ***,new discoveries to decrease oxidative stress and cellular apoptosis,inhibit aging progress,and improve neural repair in neurodegeneration and ischemic brain injury have been discussed in detail through in vitro cell culture and in vivo animal ***,the molecular mechanisms of LBPs in playing neuroprotective roles are further ***,we discuss the perspective of LBPs as biomedical compounds in TCM and modern medicine and provide the experimental and theoretical evidence to use LBPs for the treatment of aging-related neurological diseases and stroke-induced neural injuries.

关键词： Lycium barbarum polysaccharide oxidative stress neuroprotection neurodegenerative disease ischemic brain injury neural stem cell

在线全文

学校读者我要写书评

暂无评论

应用牙源干细胞进行组织损伤修复的研究进展

中国细胞生物学学报 2014年第12期36卷 1680-1688页

作者：邓琴南张彬高君宇韩发彬 Dr.Samuel QN.Deng Dental Clinic San FranciscoCA94123USA 聊城市人民医院泰山医学院聊城临床学院山东省口腔颌面医学生物学重点实验室泰山医学院聊城临床学院干细胞与再生医学重点实验室

牙源干细胞是由人类牙齿及其周围相关组织中分离出的间充质干细胞。自2000年从牙髓组织中发现和分离出牙髓干细胞以来,已有7种牙源干细胞被分离和鉴定。近年来,学者不但对这些干细胞的分离、鉴定、生物学特征和功能进行了大量基础方面... 详细信息

牙源干细胞是由人类牙齿及其周围相关组织中分离出的间充质干细胞。自2000年从牙髓组织中发现和分离出牙髓干细胞以来,已有7种牙源干细胞被分离和鉴定。近年来,学者不但对这些干细胞的分离、鉴定、生物学特征和功能进行了大量基础方面的研究,而且对其临床应用也作了广泛的探讨。该文对牙源干细胞在口腔科学领域的应用,如牙体、牙髓和牙周组织的修复和再造的最新研究进行综述,并对它们在全身疾病的治疗潜能方面作概括的介绍,如在治疗脑血管意外损伤、脊髓神经损伤、帕金森氏病、心肌梗死、糖尿病和免疫缺陷性疾病等方面的研究,以促进牙源干细胞在基础与临床应用方面的深入研究。

关键词：牙源干细胞牙髓干细胞口腔颌面牙周组织组织修复

枸杞多糖保护人脐带间充质干细胞对抗氧化损伤研究

在线全文

学校读者我要写书评

暂无评论

中医药信息 2023年第1期40卷 9-13页

作者：王程韩发彬山东中医药大学山东济南250300 山东省聊城市人民医院组织工程与再生医学实验室山东聊城252000

目的:探讨枸杞多糖(LBP)对人脐带间充质干细胞(HUC-MSCs)的抗氧化损伤保护作用。方法:采用200μmol/L H2O2刺激HUC-MSCs 6 h建立氧化损伤模型。细胞随机分为正常对照组(NC)、H2O2处理的模型组(M)、VC处理的阳性药对照组(PC)、枸杞多糖... 详细信息

目的:探讨枸杞多糖(LBP)对人脐带间充质干细胞(HUC-MSCs)的抗氧化损伤保护作用。方法:采用200μmol/L H2O2刺激HUC-MSCs 6 h建立氧化损伤模型。细胞随机分为正常对照组(NC)、H2O2处理的模型组(M)、VC处理的阳性药对照组(PC)、枸杞多糖低剂量组(LBP-L)、枸杞多糖中剂量组(LBP-M)和枸杞多糖高剂量组(LBP-H)。用细胞计数试剂盒(CCK-8)检测细胞活力,荧光分光光度计检测细胞内活性氧(ROS)水平。RT-PCR技术分析细胞凋亡相关基因Bax、Caspase-3和Caspase-9表达水平。结果:经200μmol/L H2O2刺激HUC-MSCs 6 h后,细胞体积缩小,呈明显的凋亡样变,表明HUC-MSCs氧化损伤模型建立成功;CCK-8实验结果显示,与NC组比较,M组的细胞存活率明显降低(P <0.01);与M组相比,LBP处理组细胞存活率逐渐升高(P <0.05)。ROS实验结果表明,与NC组比较,M组ROS水平明显上升(P <0.01);与M组比较,LBP-M组和LBP-H组ROS水平均明显降低(P <0.01)。RT-PCR结果显示,与NC组比较,M组中Bax、Caspase-9、Caspase-3的mRNA表达均显著上升(P <0.01);与M组比较,LBP处理组Bax、Caspase-9和Caspase-3 mRNA的表达均显著下降(P <0.05)。结论:LBP能够对氧化损伤的HUC-MSCs起到保护作用。

关键词：枸杞多糖人脐带间充质干细胞抗氧化凋亡

Epha4基因通过FGF信号通路调节大脑皮层发育

在线全文

学校读者我要写书评

暂无评论

中华细胞与干细胞杂志（电子版） 2017年第5期7卷 259-264页

作者：陈清法韩发彬聊城市人民医院组织工程与再生医学研究所 252000

目的阐明Epha4基因在大脑皮层发育早期放射性胶质细胞(RGCs)命运决定中的功能。方法利用Cre-loxp技术分别在孕龄为11.5 d或13.5 d的小鼠皮层细胞中敲掉Epha4基因。根据小鼠的基因型分为3组:对照组、Nestin;Epha4fx/fx及GFAP;Epha4fx/fx... 详细信息

目的阐明Epha4基因在大脑皮层发育早期放射性胶质细胞(RGCs)命运决定中的功能。方法利用Cre-loxp技术分别在孕龄为11.5 d或13.5 d的小鼠皮层细胞中敲掉Epha4基因。根据小鼠的基因型分为3组:对照组、Nestin;Epha4fx/fx及GFAP;Epha4fx/fx,分别通过尼氏染色、免疫荧光共染色及免疫印迹分析了突变小鼠大脑皮层表现型、大脑皮层RGCs增殖和分化及皮层细胞信号通路。组间比较利用F检验进行统计学分析。结果尼氏染色结果表明,与正常新生小鼠皮层(709±30)μm相比,在孕龄11.5 d Epha4基因敲除新生小鼠大脑皮层厚度减少至(475±66)μm,差异具有统计学意义(t=7.252,P<0.05),而孕龄13.5 d Epha4基因敲除新生小鼠大脑皮层厚度(727±37)μm,差异无统计学意义。Brd U标记及免疫荧光共染色结果表明,在孕龄14.5 d两种突变小鼠中RGCs增殖能力减弱(46±1)%vs Epha4fx/fx(58±2)%,t=10.72,P<0.05;GFAP;Epha4fx/fx(50±2)%vs Epha4fx/fx(58±2)%,t=5.575,P<0.05),而向神经元分化能力增强(Nestin;Epha4fx/fx(34±5)%vs Epha4fx/fx(25±1)%,t=4.269,P<0.05;GFAP;Epha4fx/fx(35±2)%vs Epha4fx/fx(25±1)%,t=12.48,P<0.05。Western blotting分析表明分离的皮层细胞经成纤维生长因子(FGFs)作用时ERK及FRS2α磷酸化减弱。结论 Epha4基因决定大脑皮层发育早期RGCs的命运,这一过程是通过FGFs信号通路完成的。这些发现为临床治疗脊髓损伤及阿尔茨海默症等神经疾病提供了理论依据。

关键词：大脑皮层胶质细胞 Epha4 FGF 信号通路 Cre-loxp

基于CiteSpace知识图谱分析中医药治疗乳腺癌研究现状

在线全文

学校读者我要写书评

暂无评论

山东中医杂志 2023年第7期42卷 698-704页

作者：张俊丽韩发彬山东中医药大学中医药创新研究院山东济南250355

目的:分析中医药治疗乳腺癌的研究现状及热点,探索研究新趋势。方法:检索中国知网2016年至2020年收录的中医药治疗乳腺癌相关文献,应用CiteSpace 5.7.R5W软件绘制作者、机构、关键词知识图谱,进行可视化分析。结果:共纳入文献1115篇。自... 详细信息

目的:分析中医药治疗乳腺癌的研究现状及热点,探索研究新趋势。方法:检索中国知网2016年至2020年收录的中医药治疗乳腺癌相关文献,应用CiteSpace 5.7.R5W软件绘制作者、机构、关键词知识图谱,进行可视化分析。结果:共纳入文献1115篇。自2016年以来,中医药治疗乳腺癌的研究总体呈上升趋势。存在四个主要研究团队,分别以王笑民、窦建卫、田华琴和姚昶为代表;主要研究机构为北京中医药大学、南京中医药大学、广州中医药大学、辽宁中医药大学、湖南中医药大学、山东中医药大学等;关键词共现分析共得到394个关键词,频次居前三位的为“乳腺癌”“化疗”“中医药治疗”;关键词聚类分析得到“乳腺癌”“乳腺癌术后”“淋巴水肿”等8个类别;关键词突现分析得到12个突现词,近年突现的关键词是“穴位贴敷”“二仙汤”“焦虑”。结论:中医药治疗乳腺癌的研究热点是减少和防止乳腺癌的复发、转移,提高乳腺癌患者预后水平;中医外治法和情志治疗或可成为中医药治疗乳腺癌的未来研究趋势。

关键词：中医药乳腺癌 CiteSpace 可视化分析研究热点研究趋势

Comparative analysis of the glucocerebrosidase gene mutations in canadian and Chinese populations with Parkinson's disease

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Comparative analysis of the glucocerebrosidase gene mutation...

人体脱落乳牙牙髓干细胞复合纤维蛋白修复大鼠牙周骨缺损

中国神经科学学会第十届全国学术会议

作者： fabin han Zhe Yu Ting Wang Jun Xu Wei Wang Guifang Wang Lemuel Racacho Lili Zheng Li Pan Dianrong Gong Xueli Li Huaiqian Qu Fang Li Bin Zhang David Grimes Dennis Bulman Weidong Le Centre for Stem cells and Regenerative Medicine The Affiliated Liaocheng Hospital Taishan Medical University Department of Neurology Liaocheng People's Hospital The Affiliated Liaocheng Hospital Taishan Medical University Regenerative Medicine Program Ottawa Health Research Institute University of Ottawa Department of Neurology The Ottawa Hospital University of Ottawa Institute of Neurology The Affiliated Reijing Hospital Shanghai Jiaotong University School of Medicine

Objective Mutations in the glucocerebrosidase(GBA) gene are associated with Gaucher’s *** studies indicate that pathological variants in GBA have increased the risk for patients with Parkinson’s disease(PD).To characterize the mutations of the GBA gene and compare the GBA mutation rates in PD patients and control individuals from Canadian and Chinese populations,we performed a complete sequencing analysis of the 11 exons of the GBA gene in 401 familial and sporadic PD patients and 178 normal control individuals from Canada and *** Genomic DNA was isolated from the whole bloods of 401 PD patients and 178 control individuals and was amplified by PCR to cover all 11 exons of the GBA *** DNA sequencing and bioinformatics analysis were performed to detect the GBA variants in each patient and control *** analysis was performed to compare the mutation frequencies of PD patients and control individuals of Canadian and han-Chinese *** Totally 18 different GBA mutations including 10 novel mutations were identified in 40 of 401 PD patients of Canadian and han-Chinese *** GBA mutation rates in Canadian and han-Chinese PD cohorts are 12.77%and 8.62%respectively,but there is no significant difference of the GBA mutation frequencies between the Canadian and han-Chinese PD cohorts(X=1.74,P=0.201).The GBA mutation rate in han-Chinese PD patients is significantly higher that that in han-Chinese Control individuals(X=4.21,P=0.045).The frequency of the E326K and T369M mutations(6.61%) in Canadian population of PD patients is significantly higher than that(0.57%) in han-Chinese population of PD patients(X=9.359,P=0.002),suggesting the E326K and T369M mutation rate might be affected by different ethnic *** is no significant difference of the non-pathological E326K and T369M mutation rates between the PD patients and control individuals of Canadian Origin(X=0.073,P=0.819).Interestingly,the GBA mutation rate(

关键词： Parkinson disease(PD) glucocerebrosidase(GBA) sequencing mutation deletion

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国组织工程研究 2019年第13期23卷 2061-2066页

作者：徐丽宋昊康洁贾国涛陈超王伟王琰张男韩发彬聊城大学/聊城市人民医院组织工程与再生医学研究所山东省聊城市252000 聊城市人民医院儿童口腔科山东省聊城市252000 聊城市人民医院病理科山东省聊城市252000

背景:口腔颌面部是人体的重要器官,牙周骨因感染、外伤等原因造成的缺损是口腔颌面部常见疾病之一。近年来组织工程学的迅速发展为牙周骨缺损的治疗带来了希望。目的:探讨人体脱落乳牙牙髓干细胞复合纤维蛋白对牙周骨缺损的修复作用。方... 详细信息

背景:口腔颌面部是人体的重要器官,牙周骨因感染、外伤等原因造成的缺损是口腔颌面部常见疾病之一。近年来组织工程学的迅速发展为牙周骨缺损的治疗带来了希望。目的:探讨人体脱落乳牙牙髓干细胞复合纤维蛋白对牙周骨缺损的修复作用。方法:分离和培养人体脱落乳牙牙髓干细胞,并对其进行成骨定向诱导分化2周。选取72只SD大鼠(购买于南京大学-南京生物医药研究院),随机分为3组:在磨牙下缘制备4 mm×5 mm×1 mm(宽×长×深)的牙周骨缺损模型,分别移植PBS、纤维蛋白、乳牙牙髓干细胞-纤维蛋白复合物,于术后1,2,4,6周处死大鼠并分离牙周组织标本,进行组织形态学分析评价骨修复效果。结果与结论:①乳牙牙髓干细胞在体外具有明显的成骨分化能力,蛋白免疫印迹实验分析成骨分化标记物Alp和Runx2有明显升高;②在移植后2周和4周,苏木精-伊红染色显示乳牙牙髓干细胞-纤维蛋白复合物组新骨形成百分比显著高于PBS组、纤维蛋白组;③免疫组化染色显示乳牙牙髓干细胞-纤维蛋白复合物组成骨相关标记物Alp、Runx2、Ocn的表达显著高于PBS组、纤维蛋白组;④结果表明,移植的乳牙牙髓干细胞-纤维蛋白复合物能够明显促进大鼠牙周骨缺损的修复和再生,为利用组织工程法修复人体牙周组织缺损提供了实验依据。

关键词：人体脱落乳牙牙髓干细胞纤维蛋白牙周骨缺损成骨分化成骨标记物牙周组织重建国家自然科学基金牙,乳牙髓干细胞组织工程