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miR-124 Modulates Gefitinib Resistance through SNAI2 and STAT3 in Non-small Cell Lung Cancer

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第6期36卷 839-845页

作者： fa-yong HU Xiao-nian CAO Qin-zi XU Yu DENG Sen-yan LAI Jing ma Jun-bo HU Cancer Research Institute Tongji HospitalHuazhong University of Science and Technology Department of Thoracic Surgery Tongji HospitalHuazhong University of Science and Technology Department of Respiratory and Critical Care Medicine Tongji HospitalHuazhong University of Science and Technology

Gefitinib is used as a first-line treatment for advanced non-small cell lung cancer（NSCLC）.Unfortunately,most NSCLC patients inevitably develop gefitinib resistance during *** addition to EGFR mutation status,the mechanisms involved are largely *** this study,we showed that mi R-124,a tumor suppressor,was significantly down-regulated in gefitinib-resistant NSCLC patients and cell lines compared with gefitinib-sensitive patients and cell *** addition,the mi R-124 depletion induced gefitinib resistance,and mi R-124 overexpression sensitized gefitinib-resistant cells to *** analysis revealed that mi R-124 decreased SNAI2 and STAT3 expression by directly targeting their 3＇UTRs and that knocking down SNAI2 or STAT3 partly reversed the gefitinib resistance induced by mi R-124 *** data demonstrate that the mi R-124 plays a new critical role in acquired resistance to gefitinib and that the manipulation of mi R-124 might provide a therapeutic strategy for reversing acquired gefitinib resistance.

关键词： miR-124 non-small cell lung cancer gefitinib-resistance SNAI2 STAT3

160 kV碳纳米管阴极分布式X射线管研制及应用探讨

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真空电子技术 2024年第4期 26-32页

作者：唐华平陈志强何武潘劲松荆增儒王彪马法杰清华大学工程物理系北京100084 新鸿电子有限公司江苏常州213200

介绍具有多个焦点的160 kV碳纳米管阴极分布式X射线管的研制工作及无损检测初步应用。基于Opera-3D软件对线阵列布置多焦点的模拟仿真,设计了多阴极—阵列聚焦—阳极结构,研究了45个碳纳米管阴极阵列的恒流发射特性以及多焦点的各焦点... 详细信息

介绍具有多个焦点的160 kV碳纳米管阴极分布式X射线管的研制工作及无损检测初步应用。基于Opera-3D软件对线阵列布置多焦点的模拟仿真,设计了多阴极—阵列聚焦—阳极结构,研究了45个碳纳米管阴极阵列的恒流发射特性以及多焦点的各焦点尺寸一致性。该射线管阳极电压160 kV,阳极电流15 ma,可长时间连续稳定工作于2.4 kW大功率状态,45个碳纳米管阴极工作于恒流状态时,各阴极控制电压的差异可反映其一致性,测得标准差为5.2%,45个焦点的平均焦斑尺寸为1.09 mm×1.22 mm,与模拟计算结果相符,且标准差为0.02 mm×0.06 mm,具有较好的一致性。该分布式X射线管以多焦点可编程脉冲发射的方式工作,在较小焦点状态实现大功率运行,通过线对卡测试具备较高的分辨率,对小型发动机进行多个视角的静态层析快速成像测试,可获得相比传统单点DR检测更丰富的结构信息。

关键词：碳纳米管阴极分布式X射线源一致性静态层析成像大功率

Impacts of Typhoon ‘Koppu' on Infectious Diarrhea in Guangdong Province,China

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Biomedical and Environmental Sciences 2015年第12期28卷 920-923页

作者： WANG Wei XUN Huan Miao ZHOU mai Geng JIANG Bao fa WANG Song Wang GUO Qing KANG Rui Hua WANG Xin marley Gifty ma Wei Department of Epidemiology School of Public HealthShandong University National Center for Chronic and Non-communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention National Center for Public Health Surveillance and Information Services Chinese Center for Disease Control and Prevention

This study aims to quantify the impact of typhoon‘Koppu’on infectious diarrhea in Guangdong,China in *** ratios(RRs)were calculated by comparing person-time of infectious diarrhea cases between typhoon period and reference *** of dysentery and other infectious diarrhea increased to a.ma.imum of 1.12(95%CI,0.86-1.47),1.10(95%CI,1.00-1.20)on the

关键词： Koppu Impacts of Typhoon

Staurosporine targets the Hippo pathway to inhibit cell growth

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Journal of Molecular Cell Biology 2018年第3期10卷 267-269页

作者： Xueyan ma Peixue Li Peihao Chen Jinhui Li Hongling Huang Chao Wang Wenjing Li Jianping Ding Yun Zhao fa-Xing Yu Xiangbing Qi Lei Zhang State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Innovation Center for Cell Signaling Network Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 200031 China National Institute of Biological Sciences Beijing 102206 China National Center for Protein Science Shanghai State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201210 China School of Ufe Science and Technoloy ShanghaiTech University Shanghai 201210 China Children's Hospital and Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

The Hippo signaling pathway was discovered to control organ size in Drosophila through regulating cell proliferation and apoptosis （Yu et al., 2015）. Arising studies over the past two decades have defined the core c... 详细信息

关键词： Staurosporine 小径河马 apoptosis 生长房间指向核心部件

Comparison of Histological, Microbiological, and Molecular Methods in Diagnosis of Patients with TBLN Having Different Anti-TB Treatment Background

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Biomedical and Environmental Sciences 2017年第6期30卷 418-425页

作者： CHE Nan Ying HUANG Shao Jun ma Yan HAN Yi LIU Zi Chen ZHANG Chen MU Jing ZHAO Dan QU Yang ZHANG Hai Qing LIU Zhi Dong XU Shao fa Department of Pathology Beijing Tuberculosis & Thoracic Tumor Research Institute Beijing Chest Hospital Capital Medical University Beijing 101149 China Department of Thoracic Surgery The affiliated hospital of Inner Mongolia Medical University Hohhot 010110 Inner Mongolia China Clinical Center Beijing Tuberculosis & Thoracic Tumor Research Institute Beijing Chest Hospital Capital Medical University Beo'ing 101149 China Department of Thoracic Surgery Beijing Tuberculosis & Thoracic Tumor Research Institute Beijing Chest Hospital Capital Medical University Beijing 101149 China

Objective The influence of anti-tuberculosis （TB） treatment history on tuberculous lymphadenitis （TBLN） diagnosis is unclear. Therefore, this study aims to evaluate the diagnostic methods, including histology, microbiology, and molecular tests, used for TBLN. Methods In this study, suspected patients with TBLN and having different anti-T8 treatment background were enrolled. All the samples were tested simultaneously by histology, ZiehI-Neelsen （ZN） staining, mycobacterial culture （culture）, Xpert MTB/RIF （xpert）, real-time PCR, and high-resolution melting curve PCR （HRM）. Thereafter, the performance of these methods on samples with different anti-TB treatment background was assessed. Results In our study, 89 patients were prospectively included 82 patients with TBLN and 7 with other diseases. The overall sensitivities of Xpert, real-time PCR, histology, ZN staining, and culture were 86.6%, 69.5%, 58.5%, 43.9%, and 22.0%, respectively. The anti-TB treatment history revealed dramatic influences on the sensitivity of culture （P 〈 0.0001）. In fact, the treatment that lasted over 3 months also influenced the sensitivity of Xpert （P 〈 0.05）. However, the treatment history did not affect the performance of remaining tests （P 〉 0.05）. For rifampicin drug susceptibility test （DST）, the anti-T8 treatment showed only significant influence on the success rate of culture DST （P = 0.001）, but not on those of Xpert and HRM tests （P 〉 0.05）. Conclusion Other tests as welt as culture should be considered for patients with TBLN having retreatment history or over 1-month treatment to avoid false negative results.

关键词： Tuberculous lymphadenitis Mycobacterial culture Molecular test Anti-TB treatment Drugresistance

7种细菌性虫媒病病原体的反向线状印迹杂交检测技术的建立

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甘肃农业大学学报 2024年第3期59卷 31-39页

作者：李雅琪叶得河马永华甘肃农业大学动物医学院甘肃兰州730070

【目的】建立快速高效的细菌性虫媒病的检测方法。【方法】运用反向线状印迹杂交技术(reverse line blot,RLB),通过对金黄色葡萄球菌(Staphylococcus aureus)、福氏志贺菌(Shigella flexneri)、豚鼠气单胞菌(Aeromonas caviae)、创伤弧... 详细信息

【目的】建立快速高效的细菌性虫媒病的检测方法。【方法】运用反向线状印迹杂交技术(reverse line blot,RLB),通过对金黄色葡萄球菌(Staphylococcus aureus)、福氏志贺菌(Shigella flexneri)、豚鼠气单胞菌(Aeromonas caviae)、创伤弧菌(Vibrio vulnificus)、肠沙门氏菌肠亚种鼠伤寒血清型(Salmonella enterica *** serovar Typhimurium)、普通变形杆菌(Proteus vulgaris)、小肠结肠炎耶尔森菌(Yersinia enterocolitica)7种细菌的核糖体16S RNA进行序列比对,利用DNAStar和Primer premier软件设计出了用于PCR-RLB杂交反应的基因组DNA样品进行PCR扩增的通用引物(RLB-F,RLB-R),和特异性探针以及通用探针(Catch-all),通用下游引物(RLB-R)5'端用生物素标记和探针5'端连接氨基团(NH_(2)^(+))。【结果】成功建立高效、快速、特异且能同时检测7种细菌PCR-RLB的方法,并确定了通用引物最佳浓度为25μmol/L,金黄色葡萄球菌和福氏志贺菌探针的最佳浓度分别为50μmol/L,其他5种病原探针的最佳浓度为100μmol/L。使用PCR-RLB检测方法,可对上述7种细菌的检测敏感性可达到10^(-6)~10^(-11) ng/μL,远远高于PCR的10^(-3)~10^(-8) ng/μL。【结论】首次同时对7种细菌进行检测,并确定了不同引物及探针的最佳浓度。该方法不仅提高了检测效率,同时还提高了检测的灵敏度,为开发新的检测手段提供了理论依据。

关键词：细菌性虫媒病反向线状印迹杂交技术 PCR扩增

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Nb-1Zr与316L不锈钢爆炸焊高温退火扩散层的显微组织分析

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原子能科学技术 2005年第B7期39卷 160-163页

作者：马雁杨启法中国原子能科学研究院反应堆工程研究设计所北京102413

研究了Nb1Zr合金与316L不锈钢爆炸焊在1300℃退火后形成的互扩散层。该互扩散层宽度约为80μm。用透射电子显微镜(TEM)分析观测到该扩散层中有大量针状析出相产生。经选区电子衍射(SADP)技术测定,析出相为亚稳定的ζ(Nb,Ni)相,基体相为(... 详细信息

研究了Nb1Zr合金与316L不锈钢爆炸焊在1300℃退火后形成的互扩散层。该互扩散层宽度约为80μm。用透射电子显微镜(TEM)分析观测到该扩散层中有大量针状析出相产生。经选区电子衍射(SADP)技术测定,析出相为亚稳定的ζ(Nb,Ni)相,基体相为(Ni,Cr,Nb,C)Feα合金。

关键词：爆炸焊高温退火扩散层金属间化合物

Same-Sign Dilepton Signature in the Inert Doublet Model

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Chinese Physics C 2021年第7期45卷 183-194页

作者： fa-Xin Yang Zhi-Long Han Yi Jin School of Physics and Technology University of JinanShandong 250022China

In this study,we perform a detailed analysis on the same-sign dilepton signature in the inert doublet *** on the low dark matter mass region,we randomly scan the corresponding parameter *** samples allowed by various constraints are obtained,and among them are twenty benchmark points that are selected for further study on collider *** hadron colliders,the same-sign dilepton signature is produced via pp→W^(±+)+W^(±+)jj→H^(±)H^(±)jj with the leptonic decay mode H^(±)→HW^(±)(→l^(±)ν),where H represents the dark matter *** investigate the testability of this signal at the high-luminosity LHC(HL-LHC)and the proposed 27 TeV high-energy LHC(HE-LHC).According to our simulation,the HL-LHC with L=3 ab^(-1)can barely probe this ***,for the HE-LHC with L=15ab^(-1),it is promising to obtain a 5σsignificance when 250 GeV≤m__(H)^(±)-m_(H)≤300 GeV with dark matter mass m_(H)60 or 71 GeV.

关键词： dark matter inert doublet model same-sign dilepton signature LHC

苏里格气田S区块山西组山1段沉积微相特征研究

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中外能源 2024年第3期29卷 36-43页

作者：郭春伟马瑶西安石油大学地球科学与工程学院陕西西安710065 陕西省油气成藏地质学重点实验室陕西西安710065

上古生界山西组山1段为鄂尔多斯盆地苏里格气田S区块的主力产气层,厘清其沉积相特征,对指导该地区的天然气勘探具有一定意义。运用沉积学基础原理和方法,综合岩心观察、测井曲线、微量元素特征、沉积构造及古生物化石等资料,对苏里格气... 详细信息

上古生界山西组山1段为鄂尔多斯盆地苏里格气田S区块的主力产气层,厘清其沉积相特征,对指导该地区的天然气勘探具有一定意义。运用沉积学基础原理和方法,综合岩心观察、测井曲线、微量元素特征、沉积构造及古生物化石等资料,对苏里格气田S区块山1段进行系统研究。并结合单井相、沉积相连井剖面及砂地比特征,刻画山1段沉积微相展布特征。研究结果表明,研究区岩性主要为中-细砂岩,总体表现出牵引流的沉积特征,沉积环境为气候较湿润的弱还原-还原的水下沉积环境。岩心上见冲刷面、板状交错层理、平行层理和水平层理等沉积构造,古生物化石以碳化植物茎杆化石或植物叶片化石印模为主。山1段为曲流河三角洲前缘沉积,划分为水下分流河道、水下分流间湾、席状砂沉积微相,主要发育近北南向的水下分流河道砂体,为研究区最主要的储集体。

关键词：山1段曲流河三角洲前缘牵引流沉积环境沉积微相