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TuBg1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways

Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 195-209页

作者： yan Zhang Zhen-Zhen Wang An-Qi Han Ming-ya ya.g Li-Xin Zhu fa.Ming Pan Yong Wang Department of general Surgery The Second Hospital of Anhui Medical UniversityHefei 230601China Department of general Surgery The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Epidemiology and Biostatistics School of Public HealthAnhui Medical UniversityHefei 230032China The Key Laboratory of Major Autoimmune Diseases Anhui Medical UniversityHefei 230032China

Background:As reported,γ-tubulin(TuBg1)is related to the occurrence and development of various types of malignant ***,its role in hepatocellular cancer(HCC)is not *** present study was to investigate the relationship between TuBg1 and clinical parameters and survival in HCC ***:The correlation between TuBg1 and clinical parameters and survival in HCC patients was ex-plored by bioinformatics *** was used for the *** molecular function of TuBg1 was measured using colony formation,scratch assay,trans-well assay and flow *** set enrichment analysis(gSEA)was used to pick up the enriched pathways,followed by investigating the target pathways using Western *** tumor-immune system interactions and drug bank database(TISIDB)was used to evaluate TuBg1 and *** on the TuBg1-related immune genes,a prognostic model was constructed and was further validated internally and ***:The bioinformatic analysis found high expressed TuBg1 in HCC tissue,which was confirmed us-ing immunohistochemistry and Western *** silencing the TuBg1 in HCC cell lines,more g1 arrested cells were found,cell proliferation and invasion were inhibited,and apoptosis was ***,the silence of TuBg1 increased the expressions of Ataxia-Telangiectasia and Rad-3(ATR),phospho-P38 mitogen-activated protein kinase(P-P38MAPK),phospho-P53(P-P53),B-cell lymphoma-2 associated X protein(Bax),cleaved caspase 3 and P21;decreased the expressions of B-cell lymphoma-2(Bcl-2),cyclin D1,cyclin E2,cyclin-dependent kinase 2(CDK2)and *** correlation analysis of immunohistochemistry and clinical parameters and survival data revealed that TuBg1 was negatively corre-lated with the overall *** constructed immune prognosis model could effectively evaluate the ***:The increased expression of TuBg1 in HCC is associated with poor prognosis,which might be involved in the oc

关键词： TuBg1 Hepatocellular carcinoma Apoptosis Cell cycling Immunomodulators

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Overexpression of wild-type HRAS drives non-alcoholic steatohepatitis to hepatocellular carcinoma in mice

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Zoological Research 2024年第3期45卷 551-566页

作者： Chen Ling Su-Su Liu Yu-ya Wang gui-Tao Huo yan-Wei ya.g Nan Xu Hong Wang Yong Wu Yu-fa.Miao Rui Fu Yu-Wei Zhao Chang-fa.fa. College of Life Sciences Northwest UniversityProvincial Key Laboratory of Biotechnology of Shaanxi ProvinceXi’anShaanxi 710069China Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China National Center for Safety Evaluation of Drugs National Institutes for Food and Drug Control(NIFDC)Beijing 100176China Division of HIV/AIDS and Sexually Transmitted Virus Vaccines Institute for Biological Products ControlNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China Division of Laboratory Animal Monitoring Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China

Hepatocellular carcinoma(HCC),a prevalent solid carcinoma of significant concern,is an aggressive and often fa.al disease with increasing global incidence rates and poor therapeutic *** etiology and pathological progression of non-alcoholic steatohepatitis(NASH)-related HCC is multifa.torial and ***,no single animal model can accurately mimic the full NASH-related HCC pathological progression,posing considerable challenges to transition and mechanistic ***,a novel conditional inducible wild-type human HRAS overexpressed mouse model(HRAS-HCC)was established,demonstrating 100%morbidity and mortality within approximately one month under normal dietary and lifestyle *** symptoms of HCC such as ascites,thrombus,internal hemorrhage,jaundice,and lung metastasis were successfully replicated in ***-depth pathological features of NASH-related HCC were demonstrated by pathological staining,biochemical analyses,and typical marker gene *** murine anti-PD-1 and sorafenib treatment effectively prolonged mouse survival,further confirming the accuracy and reliability of the *** on protein-protein interaction(PPI)network and RNA sequencing analyses,we speculated that overexpression of HRAS may initiate the THBS1-COL4A3 axis to induce NASH with severe fibrosis,with subsequent progression to ***,our study successfully duplicated natural sequential progression in a single murine model over a very short period,providing an accurate and reliable preclinical tool for therapeutic evaluations targeting the NASH to HCC continuum.

关键词： HRAS THBS1 HCC driver fa.tor NASH Fibrosis Cirrhosis HCC Treatment evaluation

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Shuxuening Injection Inhibits Apoptosis and Reduces Myocardial Ischemia-Reperfusion Injury in Rats through PI3K/AKT Pathway

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Chinese Journal of Integrative Medicine 2024年第5期30卷 421-432页

作者： YUE Tong-tong CAO Ying-jie CAO ya-xuan LI Wei-xia WANg Xiao-yan SI Chun-ying XIA Han ZHU Ming-jun TANg Jin-fa.WANg He The First Clinical Medical College of Henan University of Chinese Medicine Zhengzhou 450046China Department of Pharmacy the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China Department of Cardiovascular Diseases the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China

Objective: To investigate the main components and potential mechanism of Shuxuening Injection(SXNI) in the treatment of myocardial ischemia-reperfusion injury(MIRI) through network pharmacology and in vivo research. Methods: The Traditional Chinese Medicine Systems Pharmacology(TCMSP) and Pharm Mapper databases were used to extract and evaluate the effective components of ginkgo biloba leaves, the main component of SXNI. The Online Mendelian Inheritance in Man(OMIM) and gene Cards databases were searched for disease targets and obtain the drug target and disease target intersections. The active ingredient-target network was built using Cytoscape 3.9.1 software. The STRINg database, Metascape online platform, and R language were used to obtain the key targets and signaling pathways of the anti-MIRI effects of SXNI. In order to verify the therapeutic effect of different concentrations of SXNI on MIRI in rats, 60 rats were first divided into 5 groups according to random number table method: the sham operation group, the model group, SXNI low-dose(3.68 mg/kg), medium-dose(7.35 mg/kg), and high-dose(14.7 mg/kg) groups, with 12 rats in each group. Then, another 60 rats were randomly divided into 5 groups: the sham operation group, the model group, SXNI group(14.7 mg/kg), SXNI+LY294002 group,and LY294002 group, with 12 rats in each group. The drug was then administered intraperitoneally at body weight for 14 days. The main biological processes were validated using in vivo testing. Evans blue/triphenyltetrazolium chloride(TTC) double staining, hematoxylin-eosin(HE) staining, terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) assay, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis were used to investigate the efficacy and mechanism of SXNI in MIRI rats. Results: Eleven core targets and 30 Kyoto Encyclopedia of genes and genomes(KEgg) pathways were selected. Among these, the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT) pathway was

关键词： myocardial ischemia reperfusion injury PI3K/AKT signaling pathway Shuxuening Injection apoptosis ginkgo biloba leaves

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Washed microbiota transplantation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabolomic-based study

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World Journal of gastroenterology 2024年第11期30卷 1572-1587页

作者： Shi-Ju Chen Da-ya Zhang Xia Wu fa.Ming Zhang Bo-Ta Cui Yi-Hao Huang Zu-Lun Zhang Rui Wang Fei-Hu Bai graduate School Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Microbiota Medicine&Medical Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of gastroenterology The Second Affiliated Hospital of Hainan Medical UniversityHaikou 570216Hainan ProvinceChina

BACKgROUND Fecal microbiota transplantation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transplantation(WMT).Most existing studies have focused on observing the clinical ***,the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome,metatranscriptome,and metabolome-has not yet been *** To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to *** We conducted a prospective,open-label,single-center clinical *** CD patients underwent *** clinical responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabolome profiles were evaluated three months after the *** Seven of the 11 patients(63.6%)showed an optimal clinical response three months *** microbiome diversity significantly increased after WMT,consistent with improved clinical *** of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains,such as fa.calibac-terium prausnitzii,Roseburia intestinalis,and Escherichia *** addition,metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment compared to the ***,levels of vital amino acids that may be associated with disease progression(e.g.,L-glutamic acid,gamma-glutamyl-leucine,and prolyl-glutamine)were reduced after *** WMT demonstrated therapeutic efficacy in CD treatment,likely due to the effective reconstruction of the patient’s ***-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

关键词： Crohn’s disease Clinical trials Fecal microbiota transplant Metagenome Metatranscriptome Metabolome

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Promoted monolithic perovskite/organic tandem solar cells through elaborate manipulation of light transmission and carrier tunneling in interconnect junction

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Science China Chemistry 2024年第9期67卷 3131-3139页

作者： Rongbo Wang Jiawei Zhang Juntao Zhao ya Wang Yi Ding Ying Zhao Xiaodan Zhang guofu Hou Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage CenterSolar Energy Conversion CenterNankai UniversityTianjin 300350China Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Tianjin 300350China Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Tianjin 300350China State Key Laboratory of Photovoltaic Materials and Solar Cells Tianjin 300350China

Monolithic perovskite/organic tandem solar cells(TSCs)have emerged as promising thin film solar *** is recognized that interconnect junction plays a pivotal role in tandem ***,wide bandgap Cs_(0.25)fa.(0.75)Pb(I_(0.6)Br_(0.4))_(3)perovskite top-cell and narrow bandgap PM6:Y6:PC_(61)BM ternary organic bottom-cell were integrated in this study with several kinds of thin metal interconnect layers,which provides feasibility to elaborately manipulate light transmission and carrier tunneling process in interconnect *** is confirmed that,in comparison with Au,employing an Ag interconnect layer elevates integrated transmittance of light in longer wavelength regions,mainly because of the alleviated screening effect with a lower free electron concentration,which offers sufficient light harvest for the ***,established energy barriers with moderate height afford convenient extraction and recombination for both holes and ***,the performance of TSCs is promoted ***,an innovative Ag/Au double interconnect layer is proposed accordingly,which can preserve exceptional conductivity and light transmission and further reduce barrier height,especially for hole tunneling,by optimizing the band alignment between the interconnect layer and ***,the monolithic perovskite/organic TSC with a striking efficiency of 23.26% is *** a word,this study can pave a general approach toward high-performance TSCs integration.

关键词： tandem solar cells band alignment perovskite organic solar cells interconnect junction

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融合环形统计矢量的复合材料褶皱缺陷超声相位迁移成像

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物理学报 2024年第17期73卷 205-215页

作者：刘淑倩张海燕张辉朱文发陈祎婷刘雅洁上海大学通信与信息工程学院上海200444 上海工程技术大学城市轨道交通学院上海201620

超声波的相位变化携带着组织结构的重要信息,通过相位加权可提高超声图像的清晰度.针对褶皱散射回波微弱、受噪声影响大、时域修正成像耗时长等问题,提出一种基于相位虚部相干因子的频域相干成像方法.首先提取波场信号中的相位信息,然... 详细信息

超声波的相位变化携带着组织结构的重要信息,通过相位加权可提高超声图像的清晰度.针对褶皱散射回波微弱、受噪声影响大、时域修正成像耗时长等问题,提出一种基于相位虚部相干因子的频域相干成像方法.首先提取波场信号中的相位信息,然后采用环形统计获取相位虚部矩阵,通过相位虚部矩阵与相位迁移成像(phase shift migration,PSM)中的原频域矩阵相乘构建相位虚部相干因子(phase imaginary coherence fa.tor,PICF),将相位虚部相干因子引入相位迁移成像,并对各层迁移波场进行修正,通过频域信号相乘恢复纤维纹理信息.对厚度为18 mm的碳玻纤维复合材料板进行检测,实验结果表明:未加权的相位迁移成像在10 mm深度以后的纤维铺层信息丢失,无法检测出深层区域的缺陷;用PICF加权后PSM成像可检测出位于11,15,16 mm处的3个纤维褶皱,褶皱缺陷成像清晰度和纹理细致度均得到提高,褶皱角度的检测误差约为10%,并且成像耗时仅为1.5 s,运算效率比时域全聚焦成像(total focusing method,TFM)至少提高8.67倍.

关键词：环形统计矢量相位迁移成像褶皱复合材料

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周期性孔隙水压作用下砂岩损伤劣化特性研究

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岩土力学 2024年第7期45卷 2000-2010页

作者：李洪亚马林建刘杰李增邓家军许宏发陆军工程大学爆炸冲击防灾减灾全国重点实验室江苏南京210007 三峡大学三峡库区地质灾害教育部重点实验室湖北宜昌443002

消落带砂岩由于受到周期性水压渗透的影响,其物理力学特性随时间损伤劣化,导致岸坡稳定性降低。开展了周期性孔隙水压力损伤砂岩的不排水三轴压缩试验,分析了砂岩强度、变形及脆性特征演化规律,讨论了细微观结构劣化机制。结果表明:砂... 详细信息

消落带砂岩由于受到周期性水压渗透的影响,其物理力学特性随时间损伤劣化,导致岸坡稳定性降低。开展了周期性孔隙水压力损伤砂岩的不排水三轴压缩试验,分析了砂岩强度、变形及脆性特征演化规律,讨论了细微观结构劣化机制。结果表明:砂岩峰值强度随着孔隙水压作用周次的增大而减小,且强度劣化度随围压增大逐渐降低。随着孔隙水压作用周次增大,黏聚力和内摩擦角分别呈现指数式衰减和线性衰减,其中黏聚力下降是强度劣化的主要原因。弹性模量、破坏应变和Tarasov脆性指数均随孔隙水压作用周次增大逐渐降低,而随围压增大具有明显增强效应。破坏模式由单斜面剪切破坏发展至共轭剪切破坏,破裂角由67.20°逐渐减小至62.05°。周期性孔隙水压作用在裂隙尖端导致的应力集中和应力疲劳加剧了砂岩内部结构劣化,且不排水压缩过程中的孔隙水压力的增长进一步加剧了砂岩的损伤劣化,而围压作用导致部分渗流通道闭合,降低了砂岩的渗流损伤。

关键词：库岸边坡砂岩应力-渗流耦合周期性孔隙水压脆性指数损伤劣化

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上海软X射线自由电子激光单脉冲成像定时的设计与实现

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物理学报 2024年第12期73卷 89-95页

作者：聂勇敢高梓宸佟亚军范家东刘功发江怀东中国科学技术大学国家同步辐射实验室合肥230029 上海科技大学大科学中心上海201210

上海软X射线自由电子激光装置(SXFEL)是我国首台X射线自由电子激光用户装置,目前建有2条波荡器线、2条光束线以及5个实验站.装置可提供2—15 nm波长(80—620 eV)的X射线脉冲,用于高时空分辨的前沿科学研究.利用XFEL高亮度、短脉冲和全... 详细信息

上海软X射线自由电子激光装置(SXFEL)是我国首台X射线自由电子激光用户装置,目前建有2条波荡器线、2条光束线以及5个实验站.装置可提供2—15 nm波长(80—620 eV)的X射线脉冲,用于高时空分辨的前沿科学研究.利用XFEL高亮度、短脉冲和全相干的特性实现单脉冲相干衍射成像,可以有效地减轻辐射损伤,提高图像的空间分辨率.SXFEL设计重复频率为50 Hz,实现单脉冲成像的关键在于通过定时系统能够精确地控制X射线脉冲到达样品点的时间,以确保只有一个脉冲被选中用于成像.同时,还需要与成像系统的触发进行同步,以确保成像系统在正确的时间采集X射线脉冲与样品作用后的图像.本文介绍了SXFEL单脉冲成像定时的设计与实现.通过单脉冲成像的结果表明该定时方案能满足在50 Hz的SXFEL开展单脉冲成像的需求.

关键词：相干X射线衍射成像 X射线自由电子激光单脉冲成像定时系统

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基于视觉指标的雾天高速公路限速值

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公路交通科技 2024年第7期41卷 194-206页

作者：何富勇杨雅钧张驰王世法肖锋四川成绵苍巴高速公路有限责任公司成都分公司四川成都610000 四川省交通勘察设计研究院有限公司四川成都610047 长安大学公路学院陕西西安710064 四川雅康高速公路有限责任公司四川雅安625000 四川藏区高速公路有限责任公司四川成都610047

为解决雾天高速公路能见度降低和驾驶人视距受限的交通安全问题,提出了一种基于驾驶人视觉指标的限速控制方法。首先,对雾天高速公路行驶速度与驾驶人视觉的变化进行了分析,并确定了能综合表征路况信息和能见度水平的视觉指标;其次,通过... 详细信息

为解决雾天高速公路能见度降低和驾驶人视距受限的交通安全问题,提出了一种基于驾驶人视觉指标的限速控制方法。首先,对雾天高速公路行驶速度与驾驶人视觉的变化进行了分析,并确定了能综合表征路况信息和能见度水平的视觉指标;其次,通过UC-win/Road模拟了不同能见度下的驾驶环境,并收集了视觉指标和运行速度数据,进一步分析了雾天视觉指标的影响因素;然后,使用SPSS构建了运行速度和视觉指标间的数学模型,根据能见度与视觉指标间的线性关系提出了不同能见度下的视觉指标参考值;最后,基于能见度、交通流量、视觉指标3个因素,提出了一种考虑视觉指标的多条件限速控制标准。结果表明:在能见度低于200 m的环境下,视觉指标明显低于无雾环境下的视觉指标,降低范围在0.578%~1.407%之间;视觉指标与运行速度两变量间的相关系数达到0.863,这说明雾天能见度降低和视觉指标下降对行驶速度有显著影响;预测运行速度与实测速度的相对误差平均值为2.43%,满足预测精度要求。综上所述,本研究提出的限速控制方法同时考虑了能见度、交通流量和驾驶人视觉,对于保障雾天高速公路的安全行驶具有重要的实用价值。

关键词：交通工程车速限制驾驶仿真雾天行车驾驶人视觉

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生物炭基改性材料修复土壤氯代烃污染研究进展

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应用化工 2024年第5期53卷 1180-1185页

作者：李文章李法云胡亚茹王玮上海应用技术大学生态技术与工程学院上海201418 美丽中国与生态文明研究院(上海高校智库) 上海201418 上海城市路域生态工程技术研究中心上海201418

基于我国土壤氯代烃污染现状及应用生物炭材料修复的局限性,针对生物炭性能受官能团、吸附位点数量限制和实际场地修复应用的长效性较差等问题,系统综述了生物炭不同改性方法及其对生物炭性能的改进,分析了生物炭基改性材料作为载体固... 详细信息

基于我国土壤氯代烃污染现状及应用生物炭材料修复的局限性,针对生物炭性能受官能团、吸附位点数量限制和实际场地修复应用的长效性较差等问题,系统综述了生物炭不同改性方法及其对生物炭性能的改进,分析了生物炭基改性材料作为载体固定化降解微生物菌剂的特性,并深入阐述酸碱改性、金属改性生物炭及生物炭基材料固定化微生物去除氯代烃的机理与效能,以期为生物炭作为土壤氯代烃污染修复材料提供新思路。

关键词：生物炭改性土壤修复氯代烃微生物

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