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Changes of Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Waterlogging-Tolerant and Waterlogging-Sensitive Maize Genotypes at Seedling Stage

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Agricultural Sciences in China 2010年第5期9卷 651-661页

作者： TANG Bin XU Shang-zhong ZOU Xi-ling ZHENG Yong-lian qiu fa-zhan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China

To better understand the physiological and biochemical mechanisms of waterlogging tolerance, waterlogging effects on lipid peroxidation and the activity of antioxidative enzymes were investigated in leaves and roots of two maize genotypes, HZ32 （waterlogging-tolerant） and K12 （waterlogging-sensitive）. Potted maize plants were waterlogged at the second leaf stage under glasshouse conditions. Leaves and roots were harvested 1 d before and 2, 4, 6, 8 and 10 d after the start of waterlogging treatment. Through comparing the activities of superoxide dismutase （SOD）, ascorbate peroxidase （APX）, glutathione reductase （GR）, catalase （CAT） and guaiacol peroxidase （POD） between waterlogging-tolerant and waterloggingsensitive genotype, we deduced that CAT was the most important H2O2 scavenging enzyme in leaves, while APX seemed to play a key role in roots. POD, APX, GR and CAT activities in conjunction with SOD seem to play an essential protective role in the O2^- and H2O2 scavenging process. Lipid peroxidation was enhanced significantly only in K12 （P 〈 0.001） and there was no difference （P 〉 0.05） in HZ32 up to 6 d after waterlogging stress. These results indicated that oxidative stress may play an important role in waterlogging-stressed maize plants and that the greater protection of HZ32 leaves and roots from waterlogging-induced oxidative damage results, at least in part, through the maintenance of increased antioxidant enzyme activity.

关键词： antioxidative enzymes lipid peroxidation maize (Zea mays L.) seedling stage waterlogging stress

Revealing the process of storage protein rebalancing in high quality protein maize by proteomic and transcriptomic

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Journal of Integrative Agriculture 2023年第5期22卷 1308-1323页

作者： ZHAO Hai-liang QIN Yao XIAO Zi-yi SUN Qin GONG Dian-ming qiu fa-zhan National Key Laboratory of Crop Genetic Improvement/Hubei Hongshan Laboratory/College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China

Quality protein maize(QPM)(Zea mays L.) varieties contain enhanced levels of tryptophan and lysine, exhibiting improved nutritive value for humans and livestock. However, breeding QPM varieties remains challenging due to the complex process of rebalancing storage protein. This study conducted transcriptome and proteome analyses to investigate the process of storage proteins rebalancing in opaque2(o2) and QPM. We found a weak correlation between the transcriptome and proteome, suggesting a significant modulating effect of post-transcriptional events on non-zein protein abundances in Mo17o2 and QPM. These results highlight the advantages of proteomics. Compared with Mo17, 672 differentially expressed proteins(DEPs) were identified both in Mo17o2 and QPM, and several of them were associated with storage protein, starch, and amino acid synthesis. We identified 178 non-zeins as DEPs in Mo17o2 and QPM kernels. The up-regulated non-zein DEPs were enriched in lysine, tryptophan, and methionine, which affected the protein quality. Co-expression network analysis identified regulators of storage protein synthesis in QPM, including O2,PBF1, and several transcription factors. Our results revealed how storage protein rebalancing occurs and identified nonzein DEPs that may facilitate superior-quality QPM breeding.

关键词： quality protein maize opaque2 qy27 protein body storage protein iTRAQ

Engineering inorganic lead halide perovskite deposition toward solar cells with efficiency approaching 20%

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Aggregate 2021年第1期2卷 66-83页

作者： fa-Zheng qiu Ming-Hua Li Jun-Jie Qi Yan Jiang Jin-Song Hu Beijing National Laboratory for Molecular Sciences(BNLMS) CAS Key Laboratory of Molecular Nanostructure and NanotechnologyInstitute of ChemistryChinese Academy of SciencesBeijingChina State Key Laboratory for Advanced Metals and Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijingChina Energy Materials and Optoelectronics Unit Songshan Lake Materials LaboratoryDongguanGuangdongChina School of Chemical Sciences University of Chinese Academy of SciencesBeijingChina

Inorganic perovskite materials have gained tremendous attention due to their superior chemical and thermal stability than organic-inorganic hybrid *** the past years,substantial research efforts have been devoted to developing uniform and pin-hole free inorganic perovskite films with high electronic *** a result,power conversion efficiency of inorganic perovskite solar cells(PSCs)has boosted to over 19%,which presents a promising potential for technology ***,we give a comprehensive review of the recent progress on state-of-the-art inorganic cesium lead halide based PSCs,particularly on the perovskite deposition *** show a clear roadmap to fabricate high electronic quality inorganic perovskite films by tuning the precursor crystallization kinetics,performing post-deposition treatments,and passivating surface/interface *** perovskite films prepared by these approaches exhibit not only high crystallinity,favorable morphology,and low trap densities but also improved phase *** advanced deposition approaches lay the foundation for further improving the performance and long-term operational stability of inorganic perovskite photovoltaics.

关键词： efficiency inorganic perovskite methodology solar cell stability

Predominant expression of Th1-type cytokines in primary hepatic cancer and adjacent liver tissues

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Hepatobiliary & Pancreatic Diseases International 2007年第1期6卷 63-66页

作者： qiu, fa-Bo Wu, Li-Qun Lu, Yun Zhang, Shun Zhang, Bing-Yuan Qingdao Univ Affiliated Hosp Dept Hepatobiliary Vasc Surg Qingdao 266003 Peoples R China

BACKGROUND: Research has revealed a shift towards Th2 in many types of malignant tumor, but the state of Th1/Th2 is not clear in patients with primary hepatic cancer (PHC). This study was designed to determine the expression of Th1- versus Th2-type cytokines in primary hepatic cancer and the adjacent liver tissue in order to provide evidence for treatment of the Th1/Th2 shift. METHODS: Samples were collected from 11 patients with PHC. The gene expression of Th1/Th2 cytokines was detected by reverse transcriptase polymerase chain reaction (RT-PCR) using IFN-gamma and IL-2 as Th1-type cytokine genes, and IL-4 and IL-10 as Th2-type cytokine genes. RESULTS: Th1-type cytokines were expressed in 7/11 PHCs and 9/11 adjacent liver tissues, while Th0 type cytokines occurred in 4/11 PHCs and 2/11 adjacent liver tissues. CONCLUSION: Th1-type cytokines are expressed predominantly in primary hepatic cancer and the adjacent liver tissue.

关键词： primary hepatic carcinoma cytokine gene expression reverse transcriptase polymerase chain reaction

Denitrification Losses and N_2O Emissions from Nitrogen Fertilizer Applied to a Vegetable Field

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Pedosphere 2006年第3期16卷 390-397页

作者： CAO Bing He fa-Yun Xu qiu-Ming Yin Bin CAI Gui-Xin State Key laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Graduate School of the Chinese Academy of Sciences Beijing 100039 （China） Institute of Plant Nutrition and Resources Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 （China）

A field experiment was conducted on Chinese cabbage （Brassica campestris L. ssp. pekinensis （Lour.） Olsson） in a Nanjing suburb in 2003. The experiment included 4 treatments in a randomized complete block design with 3 replicates： zero chemical fertilizer N （CK）; urea at rates of 300 kg N ha^-1 （U300） and 600 kg N ha^-1 （U600）, both as basal and two topdressings; and polymer-coated urea at a rate of 180 kg N ha^-1 （PCU180） as a basal application. The acetylene inhibition technique was used to measure denitrification （N2 ＋ N2O） from intact soil cores and N2O emissions in the absence of acetylene. Results showed that compared to （3K total denitrification losses were significantly greater （P ≤ 0.05） in the PCU180, U300, and U600 treatments,while N2O emissions in the U300 and U600 treatments were significantly higher （P ≤ 0.05） than （3K. In the U300 and U600 treatments peaks of denitrification and N2O emission were usually observed after N application. In the polymer-coated urea treatment （PCU180） during the period 20 to 40 days after transplanting, higher denitrification rates and N2O fluxes occurred. Compared with urea, polymer-coated urea did not show any effect on reducing denitrification losses and N2O emissions in terms of percentage of applied N. As temperature gradually decreased from transplanting to harvest, denitrification rates and N2O emissions tended to decrease. A significant （P ≤0.01） positive correlation occurred between denitrification （r = 0.872） or N2O emission （r = 0.781） flux densities and soil temperature in the CK treatment with a stable nitrate content during the whole growing season.

关键词： denitrification loss N2O emission polymer-coated urea urea vegetable field

Synthesis and Structures of New Borophosphates (B_(1-X)M_X)PO_4 (M=Mn,Fe,Co,Ni,Cu) with Orthorhombic Low-Cristobalite Type

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Chinese Chemical Letters 1999年第8期10卷 707-708页

作者： Cheng Hua XU Shao Jie LU Gui Ying DENG fa Li qiu (Chengdu Institute of Organic Chemistry. Chinese Academy of Sciences, Chengdu 610041) Xiamen Univ Dept Mat Sci Xiamen 361005 Peoples R China Xiamen Univ Dept Chem Xiamen 361005 Peoples R China

The first reported (B1-xMx)PO4 (M = Mn, Fe, Co, Ni, Cu) compounds were synthesized by high temperature solid state methods and the structures refined from X-ray powder data. The transition metal substitutions on the boron site stabilized the structure to the orthorhombic low-cristobalite type with average volumes per tetrahedral unit 30% larger than that in BPO4 and novel catalytic properties are expected. The magnetic data indicate the 3+ valences thr the metals.

关键词： Transition-metal borophosphate,synthesis,structure magnetic property

The Effect of Al^(3+), Na^+, NH_4^+ on Ti Content in the Preparation of Ti-ZSM-5

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Chinese Chemical Letters 1999年第8期10卷 713-716页

作者： Cheng Hua XU Shao Jie LU Gui Ying DENG fa Li qiu (Chengdu Institute of Organic Chemistry. Chinese Academy of Sciences, Chengdu 610041) Chinese Acad Sci Chengdu Inst Organ Chem Chengdu 610041 Peoples R China

The effect of Al3+, NH, Na+ on the Ti content in the preparation of Ti-ZSM-5 by the isomorphous substitution of ZSM-5 using gaseous TiCl4 as titanium resource has been investigated. It is surprisingly found that although the direct ratio exists between the Ti content and the content of the skeletal Al: y=0.08x2+0.57x+1.23(here y represents TiO2% and x represents Al2O3%). but the catalytic activities of Ti-ZSM-5 for the oxidation of styrene sharply rise with the decrease of TiO2%. The skeletal Al hinders the Ti incorporation into the framework of molecular sieves characterized by FT-IR technique. At the same . NH ion has no effect on the Ti incorporation. but Na+ ion does.

关键词： Ti-ZSM-5 Ti content styrene catalytic oxidation

PARTIAL OXIDATION OF METHANE TO FORMALDEHYDE OVER VARIOUS SILICA GELS

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Journal of Energy Chemistry 1994年第4期 459-467页

作者： DAI Han-Shong qiu fa-LiChengdu Institute of Organic Chemistry,Academa Sinica Chengdu, Sichuan 610015

Partial oxidation of methane over various silica gels using oxygenas an oxidant has been investigated. The results showed that different silica gelshad different activities for formation of HCHO over verious silica gels. Thehighest yield of HCHO is 2.14% (output is 49.72g·m·h) on the silicagel-1. The results of IR, TGA and XPS indicated that the-OH group amountwas different on various silica gels, which is considered to be related to theactivity difference for partial oxidation of methane to HCHO, and the reactionmechnism is discussed.

关键词： HCHO CO PARTIAL OXIDATION OF METHANE TO FORMALDEHYDE OVER VARIOUS SILICA GELS over

OXIDATIVE DEHYDROGENATION OF ETHANE IN WET NATURAL GAS TO ETHYLENE

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Journal of Energy Chemistry 1992年第4期 341-351页

作者： qiu fa-Li LU Shao-Jie Chengdu Institute of Organic Chemistry,Academia Sinica Chengdu,Sichuan 610015

The oxidative dehydrogenation of ethane in wet natural gas toethylene over CdO-LaO/AlOcatalyst in the presence of steam has *** ethylene selectivity and yield are 84% and 35%,respectively,under the conditions of 780℃,space velocity 2120hand CH:O:HO=1.0:0.5:*** influence of temperature,space velocity andthe ratios of CH/O/HO on the selectivity and yield is also *** macrokinetics study shows the rate equation is r=KPand the ap-parent activation energy of the reaction is 260.4kJ/*** reaction mech-anism is discussed.

关键词： CZ OXIDATIVE DEHYDROGENATION OF ETHANE IN WET NATURAL GAS TO ETHYLENE GAS