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三重基序蛋白28对乳腺癌细胞增殖能力和肿瘤免疫微环境的影响

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新乡医学院学报 2024年第4期41卷 301-308页

作者：付琳琳马保东张书娟霍彦平郑州大学附属郑州中心医院乳腺外科河南郑州450001 郑州大学附属郑州中心医院干细胞再生医学转化中心河南郑州450001 郑州大学附属郑州中心医院检验科河南郑州450001

目的探讨三重基序蛋白28(TRIM28)对乳腺癌细胞增殖和集落形成能力的影响及其与肿瘤浸润免疫细胞之间的关系。方法选择2020年9月至2022年1月在郑州大学附属郑州中心医院进行诊治的39例乳腺癌患者的石蜡包埋肿瘤组织标本及配对的癌旁邻近... 详细信息

目的探讨三重基序蛋白28(TRIM28)对乳腺癌细胞增殖和集落形成能力的影响及其与肿瘤浸润免疫细胞之间的关系。方法选择2020年9月至2022年1月在郑州大学附属郑州中心医院进行诊治的39例乳腺癌患者的石蜡包埋肿瘤组织标本及配对的癌旁邻近组织,采用免疫组织化学染色法检测乳腺癌组织及癌旁组织中TRIM28蛋白的表达;将对数生长期MCF7细胞随机分为si-control组和si-TRIM28组,si-control组细胞转染空载质粒,si-TRIM28组细胞转染敲低TRIM28的质粒;采用Western blot法检测2组MCF7细胞中TRIM28蛋白表达水平,细胞计数盒-8实验检测2组细胞增殖能力,平板克隆形成实验检测2组细胞的集落形成能力;CIBERSORT算法分析美国癌症肿瘤基因图谱(TCGA)数据库中乳腺癌组织中肿瘤浸润免疫细胞的差异及其与TRIM28蛋白的相关性;基因组富集分析(GSEA)软件分析TRIM28基因表达水平差异之间的信号通路;TISIDB数据库获取TRIM28基因相关的免疫调节因子,对其进行京都基因和基因组百科全书(KEGG)通路富集分析。结果乳腺癌组织中TRIM28蛋白阳性染色定位于细胞质中,呈棕黄色细颗粒状。TRIM28蛋白在癌旁组织中呈阴性或弱阳性表达,而在乳腺癌组织中呈强阳性表达;肿瘤组织中TRIM28蛋白表达水平显著高于癌旁组织(t=2.258,P0.05)。培养第4天,si-TRIM28组MCF7细胞的存活率显著低于si-control组(t=13.920,P<0.05)。si-TRIM28组MCF7细胞克隆形成数量显著少于si-control组(t=11.528,P<0.05)。乳腺癌组织和癌旁组织中巨噬细胞亚群、B细胞亚群、T细胞亚群、活化的自然杀伤细胞、单核细胞和嗜酸性粒细胞计数比较差异均有统计学意义(P<0.05);乳腺癌组织中TRIM28蛋白表达水平与浸润的单核细胞和调节性T细胞呈正相关(r=0.150、0.100,P<0.05),与活化的树突状细胞、活化的记忆CD4 T细胞、静息的记忆CD4 T细胞、γδT细胞呈负相关(r=-0.100、-0.160、-0.099、-0.190,P<0.05);GSEA分析结果表明,TRIM28基因参与了多种免疫/癌症相关的信号通路,包括癌症通路、T细胞受体信号通路、Notch信号通路和Wnt信号通路。从TISIDB数据库中获取了TRIM28基因相关的58个免疫调节因子;对这58个免疫调节因子进行KEGG信号通路富集分析结果显示,免疫调节因子CSF1R、CXCL12、CD27、CD40和CD40LG共同参与了T细胞受体信号通路、自然杀伤细胞介导的细胞毒性、核因子-κB信号通路等信号通路。结论TRIM28蛋白在乳腺癌中高度表达,敲低TRIM28基因可抑制乳腺癌细胞的增殖能力和集落形成能力,TRIM28蛋白可能通过多种免疫/癌症相关信号通路参与调节乳腺癌免疫微环境。

关键词：乳腺癌三重基序蛋白28 免疫微环境生物信息学

New proof of continuity of Lyapunov exponents for a class of smooth Schrödinger cocycles with weak Liouville frequencies

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Frontiers of Mathematics in China 2020年第3期15卷 467-489页

作者： linlin fu Jiahao XU Fan WU Department of Mathematics Sun Yat-sen UniversityGuangzhou 510275China Chern Institute of Mathematics and LPMC Nankai UniversityTianjin 300017China Department of Mathematics Nanjing UniversityNanjing 210093China

We reconsider the continuity of the Lyapunov exponents for a class of smooth Schrödinger cocycles with a C2 cos-type potential and a weak Liouville *** propose a new method to prove that the Lyapunov exponent is conti... 详细信息

关键词： Schrodinger cocycle Lyapunov exponent(LE) weak Liouville frequency C2 cos-type potential large deviation theorem(LDT)

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基于多维小波特征的有源雷达欺骗干扰识别

现代防御技术 2024年第3期52卷 120-127页

作者：刘一兵罗强胡然付琳琳中国人民解放军63892部队河南洛阳471003

针对跟踪工作模式下常见雷达有源欺骗干扰识别问题,通过分析雷达信号和干扰模型,提出一种基于多维小波特征的识别算法。对目标回波多个相干处理间隔中的首个脉冲重复间隔内信号采样累积,构成一维离散序列,利用Mallat算法提取多尺度小波... 详细信息

针对跟踪工作模式下常见雷达有源欺骗干扰识别问题,通过分析雷达信号和干扰模型,提出一种基于多维小波特征的识别算法。对目标回波多个相干处理间隔中的首个脉冲重复间隔内信号采样累积,构成一维离散序列,利用Mallat算法提取多尺度小波系数,计算不同尺度下小波系数的移位相关系数,并组成特征向量用于分类识别。构建不同参数的信号样本仿真,在信噪比为-5 dB时仍有高于80%的识别正确率。该算法能将距离假目标干扰和距离拖引干扰有效识别,证明了算法的准确性、通用性和严谨性。此外,针对实际情况,可以调整特征向量维度,选择更适合的母小波函数,进一步优化干扰识别效果。

关键词：雷达有源欺骗干扰离散小波变换多尺度分析特征向量干扰识别信噪比

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南充高坪隐珠寺遗址及其所存碑刻初探——兼三通碑所存碑文分析

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文化创新比较研究 2024年第13期8卷 59-63页

作者：袁子東周煜钧付琳琳肖颖符永利西华师范大学历史文化学院四川南充637002 西华师范大学法学院四川南充637002

隐珠寺位于南充市高坪区走马镇隐珠寺村内,始建于明景泰年间,清代增修续建,现存主体遗址主要为明代所建。其被时人称为“嘉陵名刹”,是一处较为完整的古建筑,于2002年被列为四川省省级文物保护单位。隐珠寺内现存有三通清代碑刻及多处题... 详细信息

隐珠寺位于南充市高坪区走马镇隐珠寺村内,始建于明景泰年间,清代增修续建,现存主体遗址主要为明代所建。其被时人称为“嘉陵名刹”,是一处较为完整的古建筑,于2002年被列为四川省省级文物保护单位。隐珠寺内现存有三通清代碑刻及多处题记,碑刻因隐珠寺在清代间历次修缮得以立下。经识读整理与分析,碑文记录有隐珠寺变迁史、寺庙修缮缘由、寺庙地产纠纷等内容,既记叙了寺庙变迁历史及与之相关的诉讼纠纷,又反映了该地区在该历史时期的大体宗教观念与价值取向,是深挖隐珠寺历史故事、活化隐珠寺历史的珍贵文字依托,也是研究清代川东北地区民众佛教观、伦理观的重要文字材料。

关键词：古建筑文物隐珠寺清代碑刻碑文高坪

An inventory model under two-level trade credit when demand is stimulated by stock level 25th

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An inventory model under two-level trade credit when demand ...

Major reactive species of ambient volatile organic compounds (VOCs) and their sources in Beijing

第25届中国控制与决策会议

作者： Mo Jiangtao fu linlin Li Zhonghui Hao Jiaqin College of Mathematics and Information Sciences Guangxi University

ISBN: (纸本)9781467355339

An inventory model for deteriorating item under two-level trade credit is presented. The demand rate is a linear function of the inventory level if it is more than a certain amount;otherwise the demand rate is a constant. The optimal cycle and order quantity are determined. Numerical examples are given to illustrate the theoretical results. Sensitive analysis of major parameters has been carried out and the implications are discussed.

关键词： inventory model two-level trade credit deteriorating items stock-dependent demand

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Science China Earth Sciences 2005年第z2期48卷 147-154页

作者： SHAO Min fu linlin LIU Ying LU Sihua ZHANG Yuanhang TANG Xiaoyan State Joint Key Laboratory of Environmental Simulation and Pollution Control College of Environmental SciencesPeking UniversityBeijing 100871China

Volatile organic compounds(VOCs)are important precursors of atmospheric chemical *** a whole mixture,the ambient VOCs show very strong chemical *** on OH radical loss rates in the air,the chemical reactivity of VOCs in Beijing was *** results revealed that alkenes,accounting for only about 15%in the mixing ratio of VOCs,provide nearly 75%of the reactivity of ambient VOCs and the C4 to C5 alkenes were the major reactive species among the *** study of emission characteristics of various VOCs sources indicated that these alkenes are mainly from vehicle exhaust and gasoline *** reduction of alkene species in these two sources will be effective in photochemical pollution control in Beijing.

关键词： alkenes radicals vehicle exhaust gasoline evaporation.

Hierarchical vectorization for facial images

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Computational Visual Media 2024年第1期10卷 97-118页

作者： Qian fu linlin Liu Fei Hou Ying He School of Computer Science and Engineering Nanyang Technological University639798SingaporeSingapore Data61 Commonwealth Scientific and Industrial Research OrganisationSydney2015Australia Interdisciplinary Graduate School Nanyang Technological University and Alibaba Group639798SingaporeSingapore State Key Laboratory of Computer Science Institute of SoftwareChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China

The explosive growth of social media means portrait editing and retouching are in high *** portraits are commonly captured and stored as raster images,editing raster images is non-trivial and requires the user to be highly *** at developing intuitive and easy-to-use portrait editing tools,we propose a novel vectorization method that can automatically convert raster images into a 3-tier hierarchical *** base layer consists of a set of sparse diffusion curves(DCs)which characterize salient geometric features and low-frequency colors,providing a means for semantic color transfer and facial expression *** middle level encodes specular highlights and shadows as large,editable Poisson regions(PRs)and allows the user to directly adjust illumination by tuning the strength and changing the shapes of *** top level contains two types of pixel-sized PRs for high-frequency residuals and fine details such as pimples and *** train a deep generative model that can produce high-frequency residuals *** to the inherent meaning in vector primitives,editing portraits becomes easy and *** particular,our method supports color transfer,facial expression editing,highlight and shadow editing,and automatic *** quantitatively evaluate the results,we extend the commonly used FLIP metric(which measures color and feature differences between two images)to consider *** new metric,illumination-sensitive FLIP,can effectively capture salient changes in color transfer results,and is more consistent with human perception than FLIP and other quality measures for portrait *** evaluate our method on the FFHQR dataset and show it to be effective for common portrait editing tasks,such as retouching,light editing,color transfer,and expression editing.

关键词： face editing vectorization Poisson editing color transfer illumination editing expression editing

Inducible regulation of GDNF expression in human neural stem cells

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Science China(Life Sciences) 2013年第1期56卷 32-39页

作者： WANG ShuYan REN Ping GUAN YunQian ZOU ChunLin fu linlin ZHANG Yu Cell Biology Room Xuanwu HospitalCapital Medical Universityand Key Laboratory of NeurodegenerationMinistry of Education

Glial cell derived neurotrophic factor （GDNF） holds promises for treating neurodegenerative diseases such as Parkinson＇s dis- ease. Human neural stem cells （hNSCs） have proved to be a suitable cell delivery vehicle for the safe and efficient introduction of GDNF into the brain. In this study, we used hNSCs-infected with a lentivirus encoding GDNF and the hygromycin re- sistance gene as such vehicles. A modified tetracycline operator 7 （tetO7） was inserted into a region upstream of the EFI-α promoter to drive GDNF expression. After hygromycin selection, hNSCs were infected with a lentivirus encoding a KRAB-tetracycline repressor fusion protein （TTS）. TTS bound to tetO7 and suppressed the expression of GDNF in hNSCs. Upon administration of doxycycline （Dox） the TTS-tetO7 complex separated and the expression of GDNF resumed. The hNSCs infected with GDNF expressed the neural stem cell specific markers, nestin and sox2, and exhibited no significant change in proliferation rate. However, the rate of apoptosis in hNSCs expressing GDNF was lower compared with normal NSCs in response to actinomycin treatment. furthermore, a higher percentage of Tuj-I positive cells were obtained from GDNF-producing NSCs under conditions that induced differentiation compared to control NSCs. The inducible expression of GDNF in hNSCs may provide a system for the controllable delivery of GDNF in patients with neurodegenerative diseases.

关键词： neural stem cells GDNF lentivirus vectors tetracycline operator

On the Stability of Orthogonal Additivity in β-Homogeneous F-Spaces

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Journal of Mathematical Research with Applications 2022年第3期42卷 289-296页

作者： Qi LIU linlin fu Yongjin LI Department of Mathematics Sun Yat-sen University

In this paper, we study the stability of the orthogonal equation, which is closely related to the results by W. Fechner and J. Sikorska in 2010. There are some differences that we consider the target space with the β-homogeneous norm and quasi-norm. Overcoming theβ-homogeneous norm and quasi-norm bottlenecks, we get some new results.

关键词： Hyers-Ulam stability β-homogeneous F-spaces quasi-Banach spaces orthogonal additivity