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Transcriptome analysis reveals different response of resistant and susceptible rice varieties to rice stripe virus infection

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Journal of Integrative Agriculture 2023年第6期22卷 1750-1762页

作者： LIU Yu LIU Wen-wen LI Li frederic francis WANG Xi-feng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Functional&Evolutionary Entomology Gembloux Agro-BioTechUniversity of LiègeGembloux 5030Belgium

Rice stripe disease,caused by rice stripe virus (RSV) which is transmitted by small brown planthopper (SBPH,Laodelphax striatellus Fallen),resulted in serious losses to rice production during the last 2 *** on the molecular differences between resistant and susceptible rice varieties and the interaction between rice and RSV remains *** this study,RNA-Seq was used to analyze the transcriptomic differences between the resistant and susceptible rice varieties at different times post RSV *** Gene Ontology (GO) annotation,the differentially expressed genes (DEGs) related to transcription factors,peroxidases,and kinases of 2 varieties at 3 time points were *** these 2 varieties,the DEGs associated with these 3 GOs were numerically less in the resistant variety than in the susceptible variety,but the expression showed a significant up-or down-regulation trend under the conditions of|log_2(Fold change)|>0&P_(adj)<0.05 by significance *** through Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation,DEGs involved in some pathways that have a contribution to disease resistance including plant hormone signal transduction and plant–pathogen interaction were *** results showed that resistance responses regulated by abscisic acid (ABA) and brassinosteroids (BR) were the same for 2 varieties,but that mediated by salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET) were *** DEGs in resistant and susceptible varieties at the 3 time points were identified in both PAMP-triggered immunity (PTI) and Effector proteintriggered immunity (ETI),with that most of the unigenes of the susceptible variety were involved in PTI,whereas most of the unigenes of the resistant variety were involved in *** results revealed the different responses of resistant and susceptible varieties in the transcription level to RSV infection.

关键词： transcriptomics resistance susceptibility rice stripe virus(RSV) infection

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A conserved odorant receptor identified from antennal transcriptome of Megoura crassicauda that specifically responds to cis-jasmone

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Journal of Integrative Agriculture 2022年第7期21卷 2042-2054页

作者： WANG Bo HUANG Tian-yu YAO Yuan frederic francis YAN Chun-cai WANG Gui-rong WANG Bing Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity Tianjin Normal UniversityTianjin 300387P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Guangdong Laboratory for Lingnan Modern Agriculture(Shenzhen Branch) Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of LiegeGembloux 5030Belgium

Herbivore-induced plant volatiles(HIPVs)play a key role in the interactions between plants and herbivorous insects,as HIPVs can promote or deter herbivorous insects’*** aphids are common and serious phloem-feeding pests in farmland ecosystems,little is known about how aphids use their sensitive olfactory system to detect *** this study,the antennal transcriptomes of the aphid species Megoura crassicauda were sequenced,and expression level analyses of *** odorant receptors(ORs)were carried *** investigate the chemoreception mechanisms that *** uses to detect HIPVs,we performed in vitro functional studies of the ORs using 11 HIPVs reported to be released by aphid-infested *** total,54 candidate chemosensory genes were identified,among which 20 genes were ***20 and McraOR43 were selected for further functional characterization because their homologs in aphids were quite conserved and their expression levels in antennae of *** were relatively *** results showed that McraOR20 specifically detected cis-jasmone,as did its ortholog ApisOR20 from the pea aphid Acyrthosiphon pisum,while McraOR43 did not respond to any of the HIPV chemicals that were *** study characterized the ability of the homologous OR20 receptors in the two aphid species to detect HIPV cis-jasmone,and provides a candidate olfactory target for mediating aphid behaviors.

关键词： Megoura crassicauda transcriptome chemosensory genes odorant receptors cis-jasmone

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Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum

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Journal of Integrative Agriculture 2022年第5期21卷 1414-1423页

作者： HUANG Tian-yu ZHANG Rui-bin YANG Lu-lu CAO Song frederic francis WANG Bing WANG Gui-rong State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Guangdong Laboratory for Lingnan Modern Agriculture(Shenzhen Branch) Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of LiegeGembloux 5030Belgium

Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host ***,less is known about the role that Ors play in the host plant *** this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in *** results showed that the homologous Ors from ***,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid ***,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 *** to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the *** functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl *** them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of *** findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.

关键词： Acyrthosiphon pisum odorant receptor phylogenetic analysis two-electrode voltage clamp trans-2-hexen-1-al

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Aphidophagous guilds on nettle （Urtica dioica） strips close to fields of green pea, rape and wheat

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Insect Science 2007年第5期14卷 419-424页

作者： AMMAR ALHMEDI ERIC HAUBRUGE BERNARD BODSON frederic francis Functional and Evolutionary Entomology Experimental Farm Gembloux Agricultural University Gembloux Belgium

The common nettle （Urtica dioica L.） is a perennial and cosmopolitan plant species and is known to be the source of food for a great diversity of insects. To understand the importance of the nettle in agro-ecosystems, a field experiment was carried out in an experimental farm at Gembloux （Belgium） to study the effect of nettle margin strips on aphid and aphidophagous populations in close field crops, namely wheat （Triticum aestivum L.）, green pea （Pisum sativum L.） and rape （Brassicae napus L.）. The aphids and related beneficial populations were weekly assessed, from March to August 2005, by visual observations in two plots per field crop. A higher abundance of aphidophagous beneficials was collected in nettle strips when compared to the field crops. Particularly, the presence of predatory anthocorids, mirids and green lacewings was observed on nettle only. Nevertheless, the most abundant aphid predatory family, the Coccinellidae, was distributed in both environments, in nettle strips and in crop fields. The field margin supported a significantly higher density ofHarmonia axyridis than the field crops. In contrast, the field crops, green pea particularly, supported a higher density of Coccinella septempunctata. The distribution of the aphidophagous species, mainly the ladybirds, was discussed in relation to the host plant and related aphid species and their potential effect on integrated pest management.

关键词： Aphidophagous ladybirds beneficials insect diversity nettle wheat greenpea rape margin strips integrated pest management Microlophium camosum Acyrthosiphonpisum Sitobion avenae

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Role of terpenes from aphid-infested potato on searching and oviposition behavior of Episyrphus balteatus

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Insect Science 2007年第1期14卷 57-63页

作者： NICOLAS HARMEL RAKI ALMOHAMAD MARIE-LAURE FAUCONNIER PATRICK DU JARDIN FRANCOIS VERHEGGEN MICHEL MARLIER ERIC HAUBRUGE frederic francis Department of Functional and Evolutionary Entomology Department of Plant Biology Department of General and Organic Chemistry Gembloux Agricultural University Gembloux Belgium

To cope with pathogen and insect attacks, plants develop different mechanisms of defence, in both direct （physical and chemical） and indirect ways （attractive volatiles to entomophagous beneficials）. Plants are then able to express traits that facilitate ＂top-down＂ control of pests by attracting herbivore predators. Here we investigate the indirect defence mechanism of potato plants by analyzing the volatile patterns of both healthy and aphid- infested plants. Important changes in the emitted terpene pattern by the Myzus persicae infested host plant were observed. Using Solid Phase MicroExtraction （SPME） and GC-MS, the （E）-fl-farnesene （EBF） appeared to be emitted by aphid-infested potato and not by healthy plants. To assess the infochemical role of these volatile releases after aphid damage on the aphidophagous predators Episyrphus balteams, the hoverfly foraging behavior was assessed using the Observer 5.0 software （Noldus, Wageningen, The Netherlands）. Aphidfree potato plants were also used as a control volatile source in the predator behavioral study. While aphid-infested plants induced efficient searching and acceptation behaviors leading to egg-laying, no kairomonal effect of healthy potato plants was observed, leading to longer immobility durations and shorter searching periods in the net cage. High oviposition rate of E. balteatus was observed when aphid-infested potato was used （mean of 48.9 eggs per laying and per female）. On the other hand, no egg was produced by the hoverfly on healthy aphid-free plants. The E. balteatus foraging and reproductive behaviors according to the volatile emission from aphid-infested plants are discussed in relation to the potential use of active infochemical molecules in integrated aphid pest management.

关键词： aphid infested behavior Episyrphus balteatus potato terpenes

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Bacteria may contribute to distant species recognition in ant-aphid mutualistic relationships

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Insect Science 2017年第2期24卷 278-284页

作者： Christophe Y. Fischer Claire Detrain Philippe Thonart Eric Haubruge frederic francis Francois J. Verheggen Georges C. Lognay Laboratory of Analytical Chemistry University of Liege Gembloux Agro-Bio Tech Passage des Deportes 2 5030 Gembloux Unit of Social Ecology Universite Libre de Bruxelles CP231 50 avenue F. Roosevelt B-1050 Brussels Walloon Center of Industrial Biology University of Liege B40 Sart-Tilman 4000 Liege 4Functional and Evolutionary Entomology University of Liege Gembloux Agro-Bio Tech Passage des Deportes 2 5030 Gembloux Belgium

Mutualistic interactions between ant and aphid species have been the subject of considerable historical and contemporary investigations, the primary benefits being clean- ing and protection for the aphids and carbohydrate-rich honeydew for the ants. Questions remained, however, as to the volatile semiochemical factor influencing this relationship. A recent study highlighted the role of bacterial honeydew volatile compounds in ant at- traction. Here, ant＇s ability to distantly discriminate 2 aphid species was investigated based on bacterial honeydew semiochemicals emissions using a two-way olfactometer. Both the mutualistic aphid Aphis fabae L. and the nonmyrmecophilous aphid Acyrthosiphon pisum Harris were found to be attractive for the ant Lasius niger L. The level of attraction was similar in both assays （control vs. one of the aphid species）. However, when given a choice between these 2 aphid species, ants showed a significant preference for Aphis fabae. Honeydew volatiles, mostly from bacterial origins, are known to be a key element in ant attraction. Using the same olfactometry protocol, the relative attractiveness of volatiles emitted by honeydews collected from each aphid species and by bacteria isolated from each honeydew was investigated. Again, ants significantly preferred volatiles released by Aphis fabae honeydew and bacteria. This information suggests that microbial honeydew volatiles enable ants to distantly discriminate aphid species. These results strengthen the interest of studying the occurrence and potential impact of microorganisms in insect symbioses.

关键词： ant aphid bacteria honeydew mutualism recognition VOC

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Testing semiochemicals from aphid, plant and conspecific： attraction of Harmonia axyridis

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Insect Science 2012年第3期19卷 372-382页

作者： Pascal D. Leroy Thomas Schillings Julien Farmakidis Stephanie Heuskin Georges Lognay Francois J. Verheggen Yves Brostaux Eric Haubruge frederic francis Departments of Functional and Evolutionary Entomology Departments of Analytical Chemistry Departments of Applied Statistics Computer Science and Mathematics University of Liege Gembloux Agro-Bio Tech Gembloux Belgium

Harmonia axyridis Pallas （Coleoptera： Coccinellidae） is an invasive specie affecting the dynamics and composition of several guilds. Nowadays, no biological control method is available to reduce the populations of this harmful coccinellid. Attractants and semiochemicals seem to be the best alternative but only few studies have tested the impact of semiochemicals on this Asian lady beetle. In this work, through wind- tunnel experiments, semiochemicals from aphids （Z,E-nepetalactone, [E]-β-farnesene, a-pinene and β-pinene）, from coccinellids （[-]-β-earyophyllene） and from the nettle Urtica dioica L. were evaluated as potential attractants. The nettle volatile compounds （[Z]-3- hexenol and [E]-2-hexenal） were extracted using a Clevenger Apparatus and identified by headspace gas chromatography-mass spectroscopy. In the wind-tunnel experiments, the main components of the aphid alarm pheromone as well as a component of the aphid sexual pheromone strongly attracted both sexes of the Asian lady beetle while （-）-β- caryophyllene only attracted few individuals and had no impact on the males. The nettle extract as well as the （Z）-3-hexenol oriented both males and females to the odor source. The （E）-2-hexenal was shown to have no effect on females even if this green leaf volatile attracted males. Because Z,E-nepetalactone was identified as the most efficient attractant in the wind-tunnel experiments, this volatile was also tested in a potato field where H. axyridis has been showed to respond to this semiochemical. This study highlighted that Z,E-nepetalactone orientated the Asian lady beetle H. axyridis under natural condi- tions, indicating that this volatile compound could certainly help for an efficient biological control approach against this invasive specie.

关键词： aphids attractant Harmonia axyridis plant semiochemicals wind-tunnel

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Performances of local poultry breed fed black soldier fly larvae reared on horse manure

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Animal Nutrition 2018年第1期4卷 73-78页

作者： Nassim Moula Marie-Louise Scippo Caroline Douny Guy Degand Edwin Dawans Jean-Francois Cabaraux Jean-Luc Hornick Rudy C.Medigo Pascal Leroy frederic francis Johann Detilleux Fundamental and Applied Research for Animal&Health Animal Production DepartmentFaculty of Veterinary MedicineUniversity of Liege Fundamental and Applied Research for Animal&Health Food Science DepartmentFaculty of Veterinary MedicineUniversity of Liege Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of Liege

In poultry,feed based on maggots,like larvae of black soldier fly(Hermetia illucens) is an attractive option to substitute current ingredients which are expensive and often in direct or indirect competition with human *** information is currently available on the utility of these larvae in poultry feed,so goals of this study were to determine whether larvae could be reared on horse manure under traditional farming conditions and to evaluate the growth performances of a local poultry fed these larvae and the fatty acids profiles of their *** freezing and thawing,larvae were introduced in the feed of Ardennaise chickens between 30 and 80 days of *** in the control group received a commercial standard feed,while those in the treatment group received the same commercial feed in which 8% was substituted with whole fresh larvae corresponding to 2% on a dry matter *** ± standard errors of larval length and weight were 20.67 ± 2.21 mm and 0.14 ± 0.02 g,*** larval percentages of dry matter and of substances extractable in diethyl ether were 24.6% and 23.1%,*** fatty acids profiles were predominantly composed of lauric acid(28.1%) and palmitic acid(22.0%).Least squares means of weekly weights of chicken,adjusted for the effects of sex,replication and initial weights,were significantly higher(P < 0.05) by 77.03 ± 53.37 g in larvae-fed than in control *** other measurements were not statistically different between larvae-fed and control chicken,including fatty acid profiles,protein content and w6/w3 ratio.

关键词： Insect farming Chicken Local breed Growth performance Meat quality Fatty acid profile

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枫长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定

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昆虫学报 2011年第9期54卷 975-981页

作者：范佳 Sophie VANDERMOTEN frederic francis 刘勇陈巨莲程登发中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193 Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of LiegePassage des Deportes2B-5030 GemblouxBelgium 山东农业大学植物保护学院山东泰安271018

枫长镰管蚜Drepanosiphum platanoidis是少数几种不分泌E-β-法尼烯(E-β-farnesene,EBF)的蚜虫之一。ApisOBP3是首个被鉴定的特异结合EBF的昆虫气味结合蛋白,来自于豌豆蚜Acyrthosiphum pisum。本研究通过基因克隆技术,在枫长镰管蚜体... 详细信息

枫长镰管蚜Drepanosiphum platanoidis是少数几种不分泌E-β-法尼烯(E-β-farnesene,EBF)的蚜虫之一。ApisOBP3是首个被鉴定的特异结合EBF的昆虫气味结合蛋白,来自于豌豆蚜Acyrthosiphum pisum。本研究通过基因克隆技术,在枫长镰管蚜体内获得2个表达ApisOBP3类似蛋白的cDNA序列,长度均为426bp,其中,5'末端的69bp为信号肽编码序列。编码的成熟蛋白分别命名为DplaOBP3-1和DplaOBP3-2,两个蛋白均由119个氨基酸残基组成,序列一致性为95.8%。与ApisOBP3的序列一致性分别为95.8%和98.3%。DplaOBP3-1,DplaOBP3-2及ApisOBP33个蛋白具有数量和位置完全一致的6个保守的半胱氨酸残基。以测得3D结构的马德拉蜚蠊Leucophaea maderae的信息素结合蛋白LmadPBP为模板比较建模,结果显示两个蛋白在疏水中心均具有与ApisOBP3一致的关键疏水残基及芳香族氨基酸残基Tyr84。利用四臂嗅觉仪测定枫长镰管蚜的嗅觉行为反应,结果显示:枫长镰管蚜残体挥发物及外源EBF对该蚜均有显著的驱避作用(P=0.01)。本研究提示,枫长镰管蚜具有种内报警信息素,该蚜残体挥发物即为其种内报警信息素;枫长镰管蚜尽管并不分泌EBF,但具有表达2种OBP3蛋白的基因,并可识别环境中的EBF且对其作出行为反应。

关键词：枫长镰管蚜气味结合蛋白报警信息素 E-β-法尼烯(EBF) cDNA克隆四臂嗅觉仪

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蛋白质激发子Hrip1对小麦黄矮病的诱抗作用

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中国生物防治学报 2020年第1期36卷 97-104页

作者：李琳王双超杨秀芬 frederic francis 邱德文中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室北京100193 比利时列日大学让布鲁农学院/昆虫功能与进化系让布鲁B-5030

小麦黄矮病是由大麦黄矮病毒(barley yellow dwarf virus,BYDV)引起的一种小麦病毒病,其传播介体是小麦蚜虫,在小麦生产中造成巨大的经济损失。近年来,植物诱导抗性作为一种新兴的植物病虫害防治措施引起了广泛的关注。蛋白质激发子Hrip... 详细信息

小麦黄矮病是由大麦黄矮病毒(barley yellow dwarf virus,BYDV)引起的一种小麦病毒病,其传播介体是小麦蚜虫,在小麦生产中造成巨大的经济损失。近年来,植物诱导抗性作为一种新兴的植物病虫害防治措施引起了广泛的关注。蛋白质激发子Hrip1可以激活多种植物的免疫防御反应诱导植物产生广谱抗性。本研究评价了Hrip1对小麦黄矮病的诱抗效果。用30μg/m L的Hrip1溶液进行小麦浸种和幼苗喷雾,随后接种BYDV,接种后第14 d,Hrip1对小麦黄矮病控制效果在50%以上,接种后第21 d控制效果仍在30%以上。实时荧光定量PCR检测结果显示,在Hrip1处理的小麦幼苗体内,BYDV外壳蛋白m RNA的数量显著低于对照组;EPG结果显示,在Hrip1处理的小麦幼苗上,麦二叉蚜寻找叶片刺吸位点和韧皮部取食位点的时间增加。以上结果表明:Hrip1能够有效地抑制BYDV在小麦体内的增殖;影响传毒媒介麦二叉蚜的取食行为,抑制其传毒能力。此外,Hrip1处理小麦能有效缓解BYDV引起的叶片黄化和植株矮化的症状。因此,Hrip1可以作为生物诱导剂综合控制小麦黄矮病。

关键词： Hrip1 大麦黄矮病毒传毒能力病毒增殖

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