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新工科背景下“超星学习通+奥尔堡PBL”模式在制药工程类课程教学中的改革与实践

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中国医学教育技术 2024年第1期38卷 97-101页

作者：孟繁钦翟凤国赵玉佳张秀萍李方娟牡丹江医学院药学院黑龙江牡丹江157011

新工业革命时代的制药产业正发生着日新月异的变革,高校对制药工程专业人才的培养目标要满足行业发展需求,加强具有工程创新能力的工程师培养。制药工程专业的工程类课程是连接理论素养与工程实践能力的桥梁,处于其课程体系的核心位置... 详细信息

新工业革命时代的制药产业正发生着日新月异的变革,高校对制药工程专业人才的培养目标要满足行业发展需求,加强具有工程创新能力的工程师培养。制药工程专业的工程类课程是连接理论素养与工程实践能力的桥梁,处于其课程体系的核心位置。为满足医药发展新业态对制药工程专业人才的需求,该研究根据制药专业工程类课程特点,围绕新工科建设内涵,从整合优化教学内容、改革创新教学方式、完善考核方式与评价体系三个层面进行基于“超星学习通+奥尔堡PBL”模式的制药工程类课程教学改革,发挥教师关键作用,拓展课程内涵,丰富教学活动,实现对学生实践能力和工程创新能力的培养,尽快完成由学生向制药工程师的转变以及高等院校与现代工厂的“对接”,达到全面提高制药工程人才培养的育人成效,对制药工程专业工程类课程教学发展起到借鉴作用。

关键词：新工科制药工程奥尔堡PBL模式教学改革

Ginsenoside Rg1 protects against ischemia-induced neuron damage by regulating the rno-miRNA-27a-3p/PPARγaxis

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BIOCELL 2023年第7期47卷 1583-1594页

作者： YUE GUAN TINGTING ZHANG JIANAN YU JIAWEI LIU WENYUAN LI YUJIA ZHENG JIALE WANG YUE LIU fengguo zhai Department of Clinical Medicine Heilongjiang Nursing CollegeHarbin150001China Department of Neurology Hongqi Hospital of Mudanjiang Medical UniversityMudanjiang157011China Department of Pharmacology Mudanjiang Medical UniversityMudanjiang157011China Department of Organic Chemistry Mudanjiang Medical UniversityMudanjiang157011China Department of Anatomy Mudanjiang Medical UniversityMudanjiang157011China Department of Rehabilitation Science Kobe University Graduate School of Health SciencesKobe654-0142Japan Institute of Natural Medicine Mudanjiang Medical UniversityMudanjiang157011China

A preliminary miRNA screening showed that expression levels of rno-miRNA-27a-3p were significantly increased in the serum and brain tissues of rats undergoing cerebral *** recent years,there is evidence of the protective capacity of the saponins extracted from panax ginseng and its primary active ingredient ginsenosideRg1oncerebral ischemic ***:Fetal rat neurons(FRNs)were cultured in glucose-and-serumfree medium and exposed to hypoxia to establish a cerebral ischemia model in vitro(oxygen and glucose deprivation model,OGD).Antioxidant indexes(CAT,SOD),inflammatory markers(MPO,TNF-αand IL-6),and the expression of apoptosis and proliferation associated proteins(NF kB-p65,Caspase 3-cleaved,BCL-2)were ***:Pre-treatment of Rg1(30–100μg/mL)could effectively inhibit the decline of antioxidant indexes(CAT,SOD)and increase in inflammatory markers(MPO,TNF-αand IL-6),and effectively inhibited the apoptosis in FRNs induced by OGD in a gradient-dependent *** mechanism analysis showed that the role of Rg1 in protecting against ischemia-induced neuron damage depends on its indirect up-regulation of PPAR protein via suppression of ***,these effects of Rg1 could be reversed by exogenous rno-miRNA-27a-3p and PPAR gene silencing in FRNs exposed to ***:To summarize,our study demonstrates that Rg1 could effectively attenuate neuronal damage caused by cerebral ischemia via the rno-miRNA-27a-3p/PPARγ***,clarification of the novel mechanism will certainly improve our previous understanding of the role of Rg1 and enhancing its level in treatments for alleviating ischemic brain injury.

关键词： Ginsenoside Rg1 rno-miRNA-27a-3p PPARγ Cerebral ischemia Neuron OGD

Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

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Neural Regeneration Research 2012年第12期7卷 917-923页

作者： fengguo zhai Xiuping Zhang Yue Guan Xudong Yang Yang Li Gaochen Song Lixin Guan Department of Pharmacology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Endocrinology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Thoracic Surgery Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Biochemistry Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China

Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.

关键词： focal cerebral ischemia microRNA apoptosis bcl-2 caspase-3 neural regeneration

Influence of Panax quinquefolium saponins on increased intracellular Ca^(2+) in PC12 cells

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Neural Regeneration Research 2009年第3期4卷 225-229页

作者： Lixin Guan Xiudong Jin Yanhui Chu Yufei Zhang Yan Wu Xin Yi fengguo zhai Men clquan Li Department of Pharmacology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Physiology -Mudanjiang Medical University Mudanjiang - 157011 Heilongjian'g Province China Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy Mudanjiang Medical University Mudanjiang 157011 Heilongjiang ProvinceChina

BACKGROUND： Previous studies have demonstrated that intracellular Ca^2＋（[Ca^2＋]） overload, excitotoxicity, free radical injury, and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic brain. OBJECTIVE： To investigate the influence of Panax quinquefo/ium saponins （PQS） on multiple factors-induced Ca^2＋ overload in the rat pheochromocytoma （PC12） cell line. DESIGN, TIME AND SETTING： Intergroup comparison, in vitro study. The experiment was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University between November 2007 and April 2008. MATERIALS- In vitro cultured PC12 cells in the logarithmic phase were assigned into blank control, model, and drug treatment groups （10 μmol/L nimodipine; 40 μg/L, 100 μg/L, and 250 μg/L PQS）. Nimodipine was purchased from Jiangsu Yangtze River Pharmacy Group Co., China; PQS （purity 〉 95%, HLPC grade） was provided by School of Basic Medical Sciences, Jilin University. Caffeine, Na2S2O4, L-glutamic acid （Glu）, Fura-2/AM, and calcium ionophore A23187 were purchased from Sigma, USA. METHODS： PC12 cells in the model and drug treatment groups were separately incubated in glucose-free Hank＇s buffered saline solution ＋ Na2S2O4 （2 mmol/L） for 6 hours, Glu （200 μmot/L） plus A23187 （0.05 μmol/L） for 6 hours, KCI （50 mmol/L） for 1 hour, and caffeine （5 mmol/L） for 3 hours to establish models of intracellular Ca^2＋ overload induced by oxygen and glucose deprivation, Glu, A23187, high K＋, or caffeine. In addition, control cells were incubated in high-glucose DMEM culture medium. MAIN OUTCOME MEASURES： [Ca^2＋]i changes in PC12 cells exposed to oxygen-glucose deprivation, Glu, A23187, high K^＋, or caffeine were detected using spectrofluorometer. RESULTS： PQS blocked the [Ca^2＋]i increase induced by oxygen-glucose deprivation, Glu, A23187, high K＋, or caffeine. In particular, high-dose PQS was most effective （P 〈 0.01）. PQS significantly inhibited Glu- or caffeine-induced [Ca^2

关键词： Panax quinquefolium saponins intracellular Ca^2+ PC12 cells oxygen-glucose deprivation Fura-2/AM

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正交试验优化红参多糖饮料配方

食品工业 2018年第12期39卷 30-33页

作者：刘佳维翟凤国高馨郭义美牡丹江医学院药学院牡丹江157011

为优化红参多糖保健饮料配方,以红参多糖为主要原料,在单因素试验的基础上,通过正交试验的方法,同时以感官评分为评价标准,优化红参多糖饮料配方。结果表明:最佳红参多糖饮料配方为:红参多糖提取液添加量0.4%,木糖醇添加量8%,柠檬酸添加... 详细信息

为优化红参多糖保健饮料配方,以红参多糖为主要原料,在单因素试验的基础上,通过正交试验的方法,同时以感官评分为评价标准,优化红参多糖饮料配方。结果表明:最佳红参多糖饮料配方为:红参多糖提取液添加量0.4%,木糖醇添加量8%,柠檬酸添加量0.20%。此配方下研究制得的红参多糖保健饮料色泽淡黄,澄清透明,分布均匀,酸甜适中,口感协调,适合饮用。

关键词：红参多糖饮料正交试验

人参皂苷Rg1抗大鼠脑缺血再灌注损伤的机制研究

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今日药学 2022年第12期32卷 905-909页

作者：谭明马雪飞于文霞刘跃王佳乐翟凤国牡丹江医学院药理教研室黑龙江牡丹江157100

目的探讨人参皂苷Rg1对脑缺血再灌注(I/R)损伤大鼠的作用及作用机制。方法40只SD大鼠随机分为假手术组、脑I/R损伤模型组、人参皂苷Rg1低剂量(30 mg·kg^(-1))组、人参皂苷Rg1高剂量(60 mg·kg^(-1))组。假手术组、脑I/R损伤模型组大鼠... 详细信息

目的探讨人参皂苷Rg1对脑缺血再灌注(I/R)损伤大鼠的作用及作用机制。方法40只SD大鼠随机分为假手术组、脑I/R损伤模型组、人参皂苷Rg1低剂量(30 mg·kg^(-1))组、人参皂苷Rg1高剂量(60 mg·kg^(-1))组。假手术组、脑I/R损伤模型组大鼠腹腔内注射等量的0.9%氯化钠注射液,人参皂苷Rg1低、高剂量组腹腔内注射不同浓度的人参皂苷Rg1。大脑中动脉闭塞手术后,对4组大鼠进行运动神经功能缺损评分的比较;酶联免疫吸附测定法(ELISA)检测各组大鼠血浆中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)以及白细胞介素1β(IL-1β)的含量;qRT-PCR技术检测各组大鼠皮层脑组织中TLR4 mRNA的表达;Western blot检测各组大鼠皮层脑组织中TLR4蛋白以及介导的通路蛋白TRIF、IRF3的表达。结果人参皂苷Rg1预处理会降低脑(I/R)损伤大鼠运动神经功能缺损评分,降低血浆TNF-α、IL-6、IL-1β的含量以及皮层脑组织TLR4 mRNA、蛋白以及TRIF、IRF3蛋白的表达,呈剂量相关。结论人参皂苷Rg1可能通过抑制TLR4/TRIF/IRF3信号通路,减少脑I/R损伤大鼠炎症因子的释放,发挥神经保护作用。

关键词：脑缺血人参皂苷Rg1 炎症

西洋参茎叶皂苷对脑缺血大鼠细胞凋亡及caspase-3表达的影响

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中药药理与临床 2008年第1期24卷 30-32页

作者：关利新翟凤国聂影包海花苏云明药理教研室牡丹江医学院牡丹江157011 基础医学部牡丹江医学院牡丹江157011 黑龙江中医药大学药理教研室哈尔滨150040

目的:研究西洋参茎叶皂苷对大鼠局灶性脑缺血损伤的保护作用及其机制。方法:采用大鼠大脑中动脉内栓线阻断法制备局灶性脑缺血损伤模型,通过HE染色、TUNEL、免疫组化及RT-PCR等方法,观察西洋参茎叶皂苷对脑缺血损伤大鼠脑组织形态学改变... 详细信息

目的:研究西洋参茎叶皂苷对大鼠局灶性脑缺血损伤的保护作用及其机制。方法:采用大鼠大脑中动脉内栓线阻断法制备局灶性脑缺血损伤模型,通过HE染色、TUNEL、免疫组化及RT-PCR等方法,观察西洋参茎叶皂苷对脑缺血损伤大鼠脑组织形态学改变,细胞凋亡及半胱氨酸天冬氨酸蛋白酶(caspase)-3mRNA和蛋白表达的影响。结果:西洋参茎叶皂苷(100、50mg/kg,ig)均可明显改善脑缺血损伤所致的大鼠脑组织病理形态学改变,抑制神经细胞凋亡,降低促凋亡基因caspase-3mRNA和caspase-3蛋白的表达。结论:西洋参茎叶皂苷对大鼠局灶性脑缺血损伤具有保护作用,其作用机制可能与抑制caspase-3表达有关。

关键词：西洋参茎叶皂苷脑缺血细胞凋亡半胱氨酸天冬氨酸蛋白酶

miR-195调控人乳腺癌细胞MCF-7增殖与凋亡的作用及机制研究

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中国医药导报 2022年第1期19卷 18-22页

作者：刘嘉祺翟凤国高金霞刘佳维杨旭东周福波李孟全牡丹江医学院药理教研室黑龙江牡丹江157011 牡丹江医学院预防医学教研室黑龙江牡丹江157011 牡丹江医学院有机化学教研室黑龙江牡丹江157011 牡丹江医学院医药研究中心黑龙江牡丹江157011

目的研究miR-195调控人乳腺癌细胞MCF-7(MCF-7)增殖与凋亡作用及机制。方法体外培养正常乳腺上皮细胞MCF-10A(MCF-10A)和MCF-7,qRT-PCR检测miR-195表达,蛋白质免疫印迹(WB)检测蛋白磷酸酶1D(PPM1D)蛋白表达。MCF-7瞬时转染,3-(4,5-二甲... 详细信息

目的研究miR-195调控人乳腺癌细胞MCF-7(MCF-7)增殖与凋亡作用及机制。方法体外培养正常乳腺上皮细胞MCF-10A(MCF-10A)和MCF-7,qRT-PCR检测miR-195表达,蛋白质免疫印迹(WB)检测蛋白磷酸酶1D(PPM1D)蛋白表达。MCF-7瞬时转染,3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法、DNA断裂原位末端标记法观察miR-195对MCF-7增殖和凋亡的影响。双荧光素酶报告基因验证miR-195与PPM1D靶向的关系。结果MCF-7组miR-195表达低于MCF-10A组,PPM1D蛋白表达高于MCF-10A组,差异有统计学意义(P0.05)。结论miR-195可能通过靶向PPM1D调控乳腺癌细胞增殖与凋亡。

关键词： miR-195 蛋白磷酸酶1D 人乳腺癌细胞MCF-7 增殖凋亡

西洋参茎叶皂苷对大鼠局灶性脑缺血损伤炎症反应的影响

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中国现代医生 2017年第19期55卷 33-35,56页

作者：任鹏宇关亚新张婷婷翟凤国关利新牡丹江医学院附属红旗医院神经内科黑龙江牡丹江157011 牡丹江医学院药理教研室黑龙江牡丹江157011

目的探讨西洋参茎叶皂苷(PQS)对大鼠局灶性脑缺血损伤的保护作用与炎症反应之间的关系。方法 SD大鼠120只,随机分为5组,对照组、模型组、西洋参茎叶皂苷(50、100、200 mg/kg)组,采用大脑中动脉阻塞法制备大鼠脑缺血再灌注损伤模型。缺... 详细信息

目的探讨西洋参茎叶皂苷(PQS)对大鼠局灶性脑缺血损伤的保护作用与炎症反应之间的关系。方法 SD大鼠120只,随机分为5组,对照组、模型组、西洋参茎叶皂苷(50、100、200 mg/kg)组,采用大脑中动脉阻塞法制备大鼠脑缺血再灌注损伤模型。缺血再灌24 h后检测大鼠神经功能损伤评分;干湿重法测定脑含水量;生化法检测脑组织髓过氧物酶(MPO)的活性;ELISA法检测大鼠脑组织及血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)的含量。结果西洋参茎叶皂苷(100、200 mg/kg)组可明显减轻缺血再灌注大鼠脑神经功能损伤和脑水肿程度,降低缺血脑组织MPO的活性,降低缺血脑组织和血清TNF-α和IL-6的含量。结论西洋参茎叶皂苷可通过降低TNF-α和IL-6炎性细胞因子的表达,减缓炎症反应,减轻脑水肿,降低大鼠脑神经功能损伤程度,进而对脑缺血再灌注损伤起到一定的保护作用。

关键词：缺血再灌注西洋参茎叶皂苷炎症肿瘤坏死因子α