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Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

Research 2022年第3期2022卷 271-282页

作者： Liangfen Cheng Fuhan Yang Longfei Tang Lelin Qian xu Chen feng Guan juan Zhang Genxi Li Center for Molecular Recognition and Biosensing School of Life SciencesShanghai UniversityShanghai 200444China Department of Urology Shanghai Tenth People's HospitalSchool of MedicineTongji UniversityShanghai 200092China College of Life Science Northwest UniversityXi’an 710127China State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences Department of BiochemistryNanjing UniversityNanjing 210093China

Evaluating tumor development is of great importance for clinic treatment and *** has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the ***,in this work,cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids,i.e.,N-glycolylneuraminic acid(Neu5Gc)and N-acetylneuraminic acid(Neu5Ac).Specifically,PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface,followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra ***,the activated Cas12a indiscriminately cleaves DNA,resulting in the release of PbS and CdS quantum dots,both of which can be simultaneously detected by anodic stripping ***,Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL,***,a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface,which can be further used as a tool to identify bladder cancer cells at different development *** method to evaluate tumor development is simple and easy to be operated,so it can be potentially applied for the detection of tumor occurrence and development in the future.

关键词： CRISPR/Cas bladder stripping

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Visualizing nanopore blinkings in parallel:a high-throughput nanopore array potential for ultra-rapid DNA sequencing

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Science Bulletin 2015年第23期60卷 2067-2068页

作者： Jing-juan xu State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing University

Upon finishing the sequencing of the first complete human genome in 2004[1],the demand for fast DNA reading with less than$1,000 has been fueled by the ever-increasing awareness for personalized medicine and clinical diagnosis[2,3].Plenty of sequencing techniques,from the original Sanger sequencing method to novel sequencing-by-synthesis technologies(Roche,Solexa,SOLi D,etc.)[3],have been developed rapidly in past decades,which are sug-

关键词： DNA测序纳米孔阵列高通量可视化超快速人类基因组测序平行临床诊断

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A modular multilevel power flow controller for meshed HVDC grids

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Science China(Technological Sciences) 2014年第9期57卷 1773-1784页

作者： xu feng xu Zheng Department of Electrical Engineering Zhejiang University

Based on the comparison of existing power flow controllers(PFC)in meshed HVDC grids,the full-bridge modular multilevel converter based PFC(MMPFC)is *** first,the general branch current calculation method of meshed HVDC grids with the PFC is presented,and then,the issue of over-voltage on the thyristor based PFC is described and *** the analysis of different operating modes of the full-bridge sub-module,the mechanism of over-voltage ride through of the MMPFC is *** control strategy of the MMPFC,which is used to control branch current and keep capacitor voltage balancing,is ***,the performance on current regulation,bidirectional operation and over-voltage ride through is simulated and verified in a built model with PSCAD/EMTDC.

关键词： meshed HVDC grids multi-terminal HVDC modular multilevel power flow controller (MMPFC) bidirectional operation over-voltage ride through full-bridge sub-module

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Signal transducer and activator of transcription is involved in the expression regulation of ecdysteroid-induced insulin-like growth factor-like peptide in the pupal wing disc of silkworm, Bombyx mori

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Insect Science 2020年第6期27卷 1186-1197页

作者： Xiao-juan Zhang Dong-Dong Li Guan-feng xu Ya-Qing Chen Si-Chun Zheng Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Applied Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

In insects,20-hydroxyecdysone(20E)and insulin-like growth factor-like peptides(IGFLPs)regulate the development o f imaginal ***,how IGFLPs are up-regulated to impact the development of the pupal wing disc is still *** this study,we investigated the expression regulation of IGFLP in the pupal wing disc of silkworm,Bombyx *** confirmed that *** IGFLP(BmIGFLP)was mainly expressed in the pupal wing disc and the expression of BmIGFLP could be significantly induced by *** analysis of BmIGFLP promoter sequence revealed three m-regulation elements(CREs)of signal transducer and activator of transcription(STAT),which is a key component in the Janus-activated kinase/STAT *** activity assays showed that two CREs enhanced the transcriptional activity o f *** mobility shift and chromatin immunoprecipitation assays demonstrated that BmSTAT proteins in the nuclear extracts o f *** pupal wing discs and BmN cells could only bind to the STAT CRE3,indicating that STAT CRE3 activated by BmSTAT enhances BmIGFLP expression at pupal *** 20E could not enhance the expression of BmSTAT,20E enhanced the nucleus translocation o f BmSTAT to bind with the STAT CRE3 in the BmIGFLP *** increase of transcriptional activity of the STAT CRE3 by overexpression of BmSTAT and addition o f 20E in BmN cells confirmed this *** together,all data indicate that BmSTAT is one o f the transcription factors activating 20E-induced BmIGFLP expression in the pupal wing disc.

关键词： Bombyx mori expression regulation IGFLP 20-hydroxyecdysone STAT

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太原市城区儿童少年手腕骨龄评价分析

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体育研究与教育 2017年第1期32卷 109-112页

作者：张俊峰毛旭娟许寒雷庞立杰汪晓阳山西体育职业学院山西太原030006 山西职工医学院山西太原030006 山西大学体育学院山西太原030006

目的:通过对山西省太原市城区部分儿童少年骨骼、身高和体重等机体发育指标的研究分析,探讨当地儿童少年骨骼发育水平与生活年龄的关系。方法:对217例生活年龄10~18岁的儿童少年的非优势手腕关节进行X光摄片,采用《中国人手腕骨发育CHN... 详细信息

目的:通过对山西省太原市城区部分儿童少年骨骼、身高和体重等机体发育指标的研究分析,探讨当地儿童少年骨骼发育水平与生活年龄的关系。方法:对217例生活年龄10~18岁的儿童少年的非优势手腕关节进行X光摄片,采用《中国人手腕骨发育CHN法》进行评分测定。结果:(1)骨龄与生活年龄呈正相关(P值均<0.05),测试者男女骨龄均值大于生活年龄;(2)7~11岁、12~13岁及15~17岁阶段女性骨龄均值明显大于男性(P<0.01);(3)男性和女性腕骨龄闭合时间平均提前1.8岁。结论:骨龄能连续评价儿童青少年的生长发育;太原市儿童少年骨龄发育有提前的趋势;体重超重或肥胖与儿童少年骨骼提早发育有相关性。

关键词：骨龄生活年龄儿童少年

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Integrated metabolomic and transcriptomic analyses revealed the distribution of saponins in Panax notoginseng

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Acta Pharmaceutica Sinica B 2018年第3期8卷 458-465页

作者： Guangfei Wei Linlin Dong juan Yang Lianjuan Zhang Jiang xu feng Yang Ruiyang Cheng Ran xu Shilin Chen Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine

Panax notoginseng is famous for its important therapeutic effects. Saponins are bioactive compounds found in different parts and developmental stages of P. notoginseng plants. Thus, it is urgently to study saponins distribution in different parts and growth ages of P. notoginseng plants. In this study,potential biomarkers were found, and their chemical characteristic differences were revealed through metabolomic analysis. High-performance liquid chromatography data indicated the higher content of saponins(i.e., Rg1, Re, Rd, and Rb1) in the underground parts than that in the aerial parts. 20(S)-Protopanaxadiol saponins were mainly distributed in the aerial parts. Additionally, the total saponin content in the 3-year-old P. notoginseng plant(188.0 mg/g) was 1.4-fold higher than that in 2-year-old plant(130.5 mg/g). The transcriptomic analysis indicated the tissue-specific transcription expression of genes, namely, PnFPS, PnSS, PnSE1, PnSE2, and PnDS, which encoded critical synthases in saponin biosyntheses. These genes showed similar expression patterns among the parts of P. notoginseng *** expression levels of these genes in the flowers and leaves were 5.2 fold higher than that in the roots and fibrils. These results suggested that saponins might be actively synthesized in the aerial parts and transformed to the underground parts. This study provides insights into the chemical and genetic characteristics of P. notoginseng to facilitate the synthesis of its secondary metabolites and a scientific basis for appropriate collection and rational use of this plant.

关键词： Panax notoginseng Saponin Growth years Metabolomic analyses Gene expression

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Parameter design for a vibration absorber with time-delayed feedback control

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Acta Mechanica Sinica 2019年第3期35卷 624-640页

作者： feng Wang Jian xu School of Aerospace Engineering and Applied Mechanics Tongji UniversityShanghai 200092China

Traditional passive vibration absorbers are effective only when their natural frequencies are close to those of the excitations. To solve this problem, a vibration absorber with time-delayed feedback control is proposed to suppress vibration of the primary system under excitation with changing frequency. Firstly, the mechanical model of the delay coupled system is established. Then, the displacement transfer ratio of the system is obtained. The stability of the system is analyzed since delay may result in destabilization. Next, in order to design the control parameters, the vibration absorption performances of the proposed time-delayed vibration absorber are studied. The vibration absorption region is shown. The results show that time-delayed feedback control is able to change the response of the system. The effective vibration absorption frequency band is adjustable by tuning the control gain and time delay. The effective frequency band can be widened when choosing appropriate control parameters. The vibration absorption performances can be greatly improved by the time-delayed absorber. In addition, the optimum control parameters are obtained. Finally, the experimental prototype is constructed. Several tests with different control parameters are taken. The experimental and analytical results match quite well.

关键词： Vibration absorber Time-delayed feedback control Vibration absorption Experiments Parameter design

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Effect of gingerol on colonic motility via inhibition of calcium channel currents in rats

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World Journal of Gastroenterology 2015年第48期21卷 13466-13472页

作者： Zheng-xu Cai xu-Dong Tang feng-Yun Wang Zhi-Jun Duan Yu-Chun Li juan-juan Qiu Hui-Shu Guo Department of Internal Medicine The First Affiliated Hospital of Dalian Medical University Department of Gastro-enterology Xiyuan HospitalChina Academy of Chinese Medical Sciences Central Laboratory The First Affiliated Hospital of Dalian Medical University

AIM: To investigate the effect of gingerol on colonic motility and the action of L-type calcium channel currents in this ***: The distal colon was cut along the mesenteric border and cleaned with Ca^(2+)-free physiological saline solution. Muscle strips were removed and placed in Ca^(2+)-free physiological saline solution, which was oxygenated continuously. Longitudinal smooth muscle samples were prepared by cutting along the muscle strips and were then placed in a chamber. Mechanical contractile activities of isolated colonic segments in rats were recorded by a 4-channel physiograph. Colon smooth muscle cells were dissociated by enzymatic digestion. L-type calcium currents were recorded using the conventional whole-cell patch-clamp ***: Gingerol inhibited the spontaneous contraction of colonic longitudinal smooth muscle in a dose-dependent manner with inhibition percentages of 13.3% ± 4.1%, 43.4% ± 3.9%, 78.2% ± 3.6% and 80.5% ± 4.5% at 25 μmol/L, 50 μmol/L, 75 μmol/L and 100 μmol/L, respectively(P < 0.01). Nifedipine, an L-type calcium channel blocker, diminished the inhibition of colonic motility by gingerol. Gingerol inhibited L-type calcium channel currents in colonic longitudinal myocytes of rats. At a 75 μmol/L concentration of gingerol, the percentage of gingerolinduced inhibition was diminished by nifedipine from 77.1% ± 4.2% to 42.6% ± 3.6%(P < 0.01). Gingerol suppressed IBa in a dose-dependent manner, and the inhibition rates were 22.7% ± 2.38%, 35.77% ± 3.14%, 49.78% ± 3.48% and 53.78% ± 4.16% of control at 0 m V, respectively, at concentrations of 25 μmol/L, 50 μmol/L, 75 μmol/L and 100 μmol/L(P < 0.01). The steady-state activation curve was shifted to the right by treatment with gingerol. The value of half activation was-14.23 ± 1.12 m V in the control group and-10.56 ± 1.04 m V in the 75 μmol/L group(P < 0.05) with slope factors, Ks, of 7.16 ± 0.84 and 7.02 ± 0.93(P < 0.05) in the control and 75 μmol/L groups, respectively. H

关键词： Gingerol Colonic motility L-type calciumchannel current Spontaneous contraction Longitudinalsmooth muscle myocytes

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A preliminary evaluation of high-performance advanced regional eta-coordinate model(H-AREM)

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Atmospheric and Oceanic Science Letters 2017年第1期10卷 1-8页

作者： CHENG Yu-feng xu You-Ping LI Li-juan WANG Bin The State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China College of Earth Science University of Chinese Academy of Sciences Beijing China CpLA Troops PLA Troops No.78127 Chengdu China Beijing Institute of Applied Meteorology Beijing China

This paper preliminarily evaluates the speedup,scalability,and prediction skill of the highperformance advanced regional eta coordinate model（H-AREM）,which is based on several parallel processing methods and decomposition *** show that the parallel version of the model that is based on a modular parallel framework and a multidimensional domain decomposition strategy performs better overall,*** is faster and more scalable than the version based on a message passing interface and a one-dimensional decomposition *** particular,the scalability of the H-AREM with a resolution of 8 km approaches 8099 ***,in the H-AREM,higher resolutions result in more realistic precipitation predictions without remarkable increases in simulation time.

关键词： AREM parallel computation speed-up scalability

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Constraint-induced movement therapy enhances angiogenesis and neurogenesis after cerebral ischemia/reperfusion

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Neural Regeneration Research 2019年第10期14卷 1743-1754页

作者： Zhi-Yong Zhai juan feng Department of Neurology Shengjing Hospital of China Medical University

Constraint-induced movement therapy after cerebral ischemia stimulates axonal growth by decreasing expression levels of Nogo-A,RhoA,and Rho-associated kinase(ROCK)in the ischemic boundary ***,it remains unclear if there are any associations between the Nogo-A/RhoA/ROCK pathway and angiogenesis in adult rat brains in pathological processes such as ischemic *** addition,it has not yet been reported whether constraint-induced movement therapy can promote angiogenesis in stroke in adult rats by overcoming Nogo-A/RhoA/ROCK ***,a stroke model was established by middle cerebral artery occlusion and *** days after stroke,the following treatments were initiated and continued for 3 weeks:forced limb use in constraint-induced movement therapy rats(constraint-induced movement therapy group),intraperitoneal infusion of fasudil(a ROCK inhibitor)in fasudil rats(fasudil group),or lateral ventricular injection of NEP1-40(a specific antagonist of the Nogo-66 receptor)in NEP1-40 rats(NEP1-40 group).Immunohistochemistry and western blot assay results showed that,at 2 weeks after middle cerebral artery occlusion,expression levels of RhoA and ROCK were lower in the ischemic boundary zone in rats treated with NEP1-40 compared with rats treated with ischemia/reperfusion or constraint-induced movement therapy ***,at 4 weeks after middle cerebral artery occlusion,expression levels of RhoA and ROCK in the ischemic boundary zone were markedly decreased in the NEP1-40 and constraint-induced movement therapy groups,but there was no difference between these two *** with the ischemia/reperfusion group,modified neurological severity scores and foot fault scores were lower and time taken to locate the platform was shorter in the constraint-induced movement therapy and fasudil groups at 4 weeks after middle cerebral artery occlusion,especially in the constraint-induced movement therapy *** staining demonstrated that fas

关键词： nerve regeneration constraint-induced movement therapy angiogenesis ischemia/reperfusion subventricular zone Nogo-A fasudil neurovascular unit tight junction protein vascular endothelial growth factor receptor 2 neural regeneration

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