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谷氨酰tRNA合成酶转基因拟南芥的生长特性分析

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四川大学学报（自然科学版） 2009年第3期46卷 838-842页

作者：祝欣何菡姜维嘉王健美李旭锋杨毅四川大学生命科学学院生物资源与生态环境教育部重点实验室成都610064

以不同独立株系的拟南芥谷氨酰tRNA合成酶(Arabidopsis glutamyl tRNA syn-thetase,简称AtGluRS)过量表达的三种纯合株系(GS3、GS4、GS6)及抑制表达的三种纯合株系(GAS2、GAS3、GAS7)为实验材料,分析其生长情况和表型。表型分析结果表明... 详细信息

以不同独立株系的拟南芥谷氨酰tRNA合成酶(Arabidopsis glutamyl tRNA syn-thetase,简称AtGluRS)过量表达的三种纯合株系(GS3、GS4、GS6)及抑制表达的三种纯合株系(GAS2、GAS3、GAS7)为实验材料,分析其生长情况和表型。表型分析结果表明,拟南芥谷氨酰tRNA合成酶过量表达植株表现出开花晚、基叶多、茎生长快等与对照和AtGluRS抑制表达植株不同的特点。此外,用氯化钠(NaCl)模拟盐胁迫环境,对转基因植物的萌发率和生长情况进行分析,发现谷氨酰tRNA合成酶的过量表达增强了植物对盐胁迫的耐受能力。因此,拟南芥谷氨酰tRNA合成酶影响植物表型并参与植物抗逆过程。

关键词：脱落酸谷氨酰 tRNA合成酶转基因植物植物抗逆

基于个体化机器学习的原发性免疫性血小板减少症危重出血预测模型:一项全国前瞻性队列研究

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Science Bulletin 2023年第18期68卷 2106-2114,M0004页

作者： zhuo-Yu An Ye-Jun Wu Yu Hou Heng Mei wei-Xia Nong Wen-Qian li Hu Zhou Ru feng jian-Ping Shen Jun Peng Hai Zhou Yi liu Yong-Ping Song lin-Hua Yang Mei-Yun Fang jian-Yong li Yun-feng Cheng Peng liu Ya-Jing Xu Zhao Wang Yi Luo Zhen Cai Hui liu Jing-Wen Wang Juan li Xi Zhang Zi-Min Sun Xiao-Yu zhu xin Wang Rong Fu liang Huang Shao-Yuan Wang Tong-Hua Yang li-Ping Su liang-Ming Ma Xie-Qun Chen Dai-Hong liu Hong-Xia Yao Jia feng Hong-Yu Zhang Ming jiang Ze-Ping Zhou Wen-Sheng Wang Xu-liang Shen Yangjin Baima yue-Ying li Qian-Fei Wang Qiu-Sha Huang Hai-Xia Fu Xiao-Lu zhu Yun He Qian jiang Hao jiang Jin Lu Xiang-Yu Zhao Ying-Jun Chang Tao Wu Yao-zhu Pan lin Qiu Da Gao A-Rong Jin wei li Su-Jun Gao Lei Zhang Ming Hou Xiao-Jun Huang Xiao-Hui Zhang on behalf of the National Cooperative ITP Working Group Peking University People’s Hospital Peking University Institute of HematologyBeijing 100044China Collaborative Innovation Center of Hematology Peking UniversityBeijing 100044China Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Beijing 100044China National Clinical Research Center for Hematologic Disease Beijing 100044China Department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250014China Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430074China Department of Hematology First Affiliated HospitalSchool of MedicineShihezi UniversityShihezi 832002China Department of Hematology Qinghai Provincial People’s HospitalXining 810007China Henan Cancer Hospital Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of HematologyZhengzhou 450008China Department of Hematology Beijing HospitalBeijing 100044China Department of Hematology Zhejiang Provincial Hospital of Traditional Chinese MedicineHangzhou 310006China Department of Hematology Senior Department of Hematologythe Fifth Medical Center of PLA General HospitalBeijing 100044China Department of Hematology the Second Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Hematology Affiliated Zhongshan Hospital of Dalian UniversityDalian 116001China Department of Hematology The First Affiliated Hospital with Nanjing Medical University(Jiangsu Province Hospital)Nanjing 210029China Department of Hematology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Hematology Xiangya HospitalCentral South UniversityChangsha 410008China Department of Hematology Beijing Friendship HospitalCapital Medical UniversityBeijing 100044China Institute of Hematology the First Affiliated HospitalZhejiang UniversityHangzhou 310058China Department of Hematology Beijing Tongren HospitalBeijing 100005China Department of Hematology The First Affiliated HospitalSun Yat-sen UniversityGuang

原发性免疫性血小板减少症(ITP)中少见但至关重要的危重出血事件,给患者的预后、生活质量和治疗决策带来严重影响。尽管有一些研究探讨了ITP中与危重出血相关的风险因素,但目前尚缺乏大样本数据、大规模多中心研究结果以及针对ITP患者... 详细信息

原发性免疫性血小板减少症(ITP)中少见但至关重要的危重出血事件,给患者的预后、生活质量和治疗决策带来严重影响。尽管有一些研究探讨了ITP中与危重出血相关的风险因素,但目前尚缺乏大样本数据、大规模多中心研究结果以及针对ITP患者致命出血事件的预测模型。本研究首次采用国际血栓与止血学会新提出的ITP致命出血标准,利用大样本数据开发了首个基于机器学习的在线应用,用于预测ITP患者的致命出血.研究中,我们使用中国各地大型多中心数据进行开发,并对全国39家医疗中心进行为期一年的外部测试,得到了较好的训练、验证和测试数据集预测能力该基于新算法的便捷网络工具能够快速识别ITP患者的出血风险,辅助临床决策,有望未来降低不良事件的发生。

关键词： Critical bleeding Severe bleeding Immune thrombocytopenia Machine learning Prediction model

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Biomarkers of aging

Science China(life Sciences) 2023年第5期66卷 893-1066页

作者： Aging Biomarker Consortium Hainan Bao jiani Cao Mengting Chen Min Chen wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping jiang Ying Jing Dingfeng li Jiaming li Jingyi li Qinhao liang Rui liang feng liu Xiaoqian liu Zuojun liu Oscar Junhong Luo jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie xinhua Qiao xinpei Sun Xiaoqiang Tang jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao lingyan Xu Yingying Xu Haoteng Yan liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang weiwei Zhang Wenwan Zhang xing Zhang zhuo Zhang Min Zhou Rui Zhou Qingchen zhu Zhengmao zhu feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen weimin Ci Bi-Sen Ding Qiurong Ding feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji li jian li xin li Baohua liu feng liu lin liu Qiang liu Qiang liu xingguo liu Yong liu Xianghang Luo Shuai Ma xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui yue Cuntai Zhang Hongbo Zhang liang Zhang weiqi Zhang Yong Zhang Yun-Wu Zhang zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai zhu weiguo Zou Gang Pei Guang-Hui liu Aging Biomarker Consortium(ABC) Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Aging Biology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Clinic Center of Human Gene Research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Clinical Research Center of Metabolic and Cardiovascular Disease Huazhong University of Science and TechnologyWuhan 430022China Hubei Key Laboratory of Metabolic Abnormalities and Vascular Aging Huazhong University of Science and TechnologyWuhan 430022China Stem Cell Translational Research Center Tongji HospitalTongji University School of MedicineShanghai 200065China Department of Nuclear Medicine Daping HospitalThird Military Medical UniversityChongqing 400042China CAS Key Laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Shanghai Key Laboratory of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China The Department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Ageing and Regenerative MedicineJ

Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological *** a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly *** immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this ***,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory *** fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.

关键词： aging senescence biomarker clock

不同提取方法对竹荪多糖提取率及抗氧化性影响

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中国食用菌 2020年第3期39卷 24-28页

作者：王荣琨王钦罗欣孙达锋蒋建新朱莉伟北京林业大学材料科学与技术学院林业生物质材料与能源教育部工程研究中心北京100083 中华全国供销合作总社昆明食用菌研究所云南昆明650223

对比了不同提取方法下竹荪多糖的提取率和抗氧化性,并进行结构初步表征。超声波辅助提取法、压力辅助提取法和不同pH水浸提法所得的纯多糖提取率依次为12.28%、7.02%和6.61%~6.81%。竹荪多糖的抗氧化活性受提取方法影响较明显,其中超声... 详细信息

对比了不同提取方法下竹荪多糖的提取率和抗氧化性,并进行结构初步表征。超声波辅助提取法、压力辅助提取法和不同pH水浸提法所得的纯多糖提取率依次为12.28%、7.02%和6.61%~6.81%。竹荪多糖的抗氧化活性受提取方法影响较明显,其中超声波辅助提取法所得多糖抗氧化性最强,对羟基自由基和DPPH自由基的清除率在浓度2 mg·mL^-1时分别可达46.53%和60.69%。通过紫外、红外和离子交换色谱检测,分析得出竹荪多糖主要由葡萄糖、半乳糖、甘露糖和木糖组成,具有典型多糖特征吸收峰。

关键词：竹荪多糖提取方法抗氧化性单糖组成

京杭大运河丹阳段及丹金溧漕河沿线钉螺时空分布特征研究

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中国血吸虫病防治杂志 2020年第1期32卷 10-14,27页

作者：蒋甜甜施亮李伟张键锋何健王鑫瑶刘璐冯云王琳谢轶青王志坚朱涛江涛孙建平戴伟许大成杨坤国家卫生健康委员会寄生虫病预防与控制技术重点实验室、江苏省寄生虫与媒介控制技术重点实验室、江苏省血吸虫病防治研究所无锡214064 江南大学公共卫生研究中心江苏省镇江市疾病预防控制中心江苏省常州市疾病预防控制中心江苏省丹阳市疾病预防控制中心江苏省常州市金坛区疾病预防控制中心

目的分析京杭大运河江苏丹阳段及丹金溧漕河沿线钉螺时空分布特征,为动态评估该区域钉螺扩散风险提供科学依据。方法收集2012-2017年研究区域内水网型血吸虫病流行区螺情数据,采用缓冲区分析、空间自相关分析、热点分析及标准差椭圆分析... 详细信息

目的分析京杭大运河江苏丹阳段及丹金溧漕河沿线钉螺时空分布特征,为动态评估该区域钉螺扩散风险提供科学依据。方法收集2012-2017年研究区域内水网型血吸虫病流行区螺情数据,采用缓冲区分析、空间自相关分析、热点分析及标准差椭圆分析法,探索京杭大运河丹阳段及丹金溧漕河沿线钉螺时空变化趋势。结果2012-2017年,研究区域内有螺环境数呈上升趋势;有螺环境主要分布于丹金溧漕河1000 m缓冲区内,特别是京杭大运河与丹金溧漕河交汇处的老九曲河沿线。2014、2016、2017年,研究区域内钉螺分布存在空间自相关,热点区域主要为丹金溧漕河与京杭大运河交汇处;钉螺分布整体呈东北-西南方向,随着时间变化逐渐向南北方向转变。结论京杭大运河丹阳段及丹金溧漕河沿线钉螺空间分布复杂,存在从上游向下游扩散的风险,需加强该区域的灭螺工作。

关键词：钉螺时空分布京杭大运河丹金溧漕河江苏省

固相萃取-高效液相色谱串联质谱测定人体尿液中芳香化合物代谢物的方法研究

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四川大学学报（医学版） 2020年第5期51卷 695-701页

作者：任建伟罗新月赵璇王璇朱婧别明江刘峻杉丛雪邹晓莉四川大学华西公共卫生学院/四川大学华西第四医院卫生检验与检疫系成都610041 四川大学学报(医学版)编辑部成都610041

目的建立固相萃取-高效液相色谱串联质谱(high performance liquid chromatographic tandem mass spectrometric, HPLC-MS/MS)同时测定尿液中8种芳香化合物代谢物(苯巯基尿酸、甲基马尿酸、N-乙酰基-S-苯基-L-半胱氨酸、苯乙醛酸、苯乙... 详细信息

目的建立固相萃取-高效液相色谱串联质谱(high performance liquid chromatographic tandem mass spectrometric, HPLC-MS/MS)同时测定尿液中8种芳香化合物代谢物(苯巯基尿酸、甲基马尿酸、N-乙酰基-S-苯基-L-半胱氨酸、苯乙醛酸、苯乙醇酸、对氨基酚、对硝基酚和1-羟基芘)的方法。方法取1 mL尿液,加入20μLβ-葡萄糖醛酸酶和1 mL乙酸铵缓冲溶液,在37℃孵箱中酶解20 h。酶解后混匀,取适量酶解液注入PrimeHLB固相萃取柱净化,4 mL乙腈洗脱,洗脱液氮吹至干,用0.20 mL甲醇复溶后进样HPLC-MS/MS系统分析。高效液相色谱分离采用反相C18色谱柱(2.1 mm×150 mm,3.5μm),流动相A为含体积分数0.1%甲酸的水,流动相B为甲醇,采用梯度洗脱,流速为0.2 mL/min。质谱采用正、负离子交替扫描方式,多反应监测(multiple reaction monitoring, MRM)模式检测,内标标准曲线法定量。结果 8种代谢物在1~100 ng/mL范围内线性良好,相关系数均大于0.995,测定的精密度(relative standard deviation, RSD)在0.05%~9.95%之间,8种代谢物的检出限在0.041~0.12 ng/mL范围内。将所建立的方法用于尿样分析,加标回收率为80.1%~114.0%。结论所建立的方法灵敏、快速、准确,适用于普通人群和职业人群的芳香化合物生物监测评估。

关键词：高效液相色谱串联质谱法固相萃取尿液芳香化合物代谢物生物监测

Searching for ^(76)Ge neutrinoless double beta decay with the CDEX-1B experiment

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Chinese Physics C 2024年第10期48卷 11-22页

作者： Bing-Tao Zhang Jun-Zheng Wang li-Tao Yang Qian yue Ke-Jun Kang Yuan-Jing li Hai-Peng An C.Greeshma jian-Ping Chang Yun-Huan Chen jian-Ping Cheng Wen-Han Dai Zhi Deng Chang-Hao Fang xin-Ping Geng Hui Gong Qiu-Ju Guo Xu-Yuan Guo li He Sheng-Ming He Jin-wei Hu Han-Xiong Huang Tu-Chen Huang Hai-Tao Jia Xi jiang S.Karmakar Hao-Bin li jian-Min li Jin li Qian-Yun li Ren-Ming-Jie li Xue-Qian li Yu-Lan li Yi-Fan liang Bin liao Fong-Kay lin Shin-Ted lin Jia-Xuan liu Shu-Kui liu Yan-Dong liu Yu liu Yuan-Yuan liu Zhong-Zhi liu Hao Ma Yu-Cai Mao Qi-Yuan Nie Jin-Hua Ning Hui Pan Ning-Chun Qi Jie Ren Xi-Chao Ruan Ze She Manoj Kumar Singh Tian-Xi Sun Chang-jian Tang wei-You Tang Yang Tian Guang-Fu Wang li Wang Qing Wang Yu-feng Wang Yun-Xiang Wang Henry-Tsz-King Wong Shi-Yong Wu Yu-Cheng Wu Hao-Yang xing Rui Xu Yin Xu Tao Xue Yu-Lu Yan Nan Yi Chun-Xu Yu Hai-Jun Yu jian-feng yue Ming Zeng Zhi Zeng feng-Shou Zhang Lei Zhang Zhen-Hua Zhang Zhen-Yu Zhang Kang-Kang Zhao Ming-Gang Zhao Ji-Fang Zhou Zu-Ying Zhou Jing-Jun Zhou Key Laboratory of Particle and Radiation Imaging(Ministry of Education)and Department of Engineering Physics Tsinghua UniversityBeijing 100084 Department of Physics Tsinghua UniversityBeijing 100084 Institute of Physics Academia SinicaTaipei 11529 NUCTECH Company Beijing 100084 YaLong River Hydropower Development Company Chengdu 610051 College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875 College of Physics Sichuan UniversityChengdu 610065 School of Physics Peking UniversityBeijing 100871 Department of Nuclear Physics China Institute of Atomic EnergyBeijing 102413 Sino-French Institute of Nuclear and Technology Sun Yat-sen UniversityZhuhai 519082 School of Physics Nankai UniversityTianjin 300071 Department of Physics Banaras Hindu UniversityVaranasi 221005 Department of Physics Beijing Normal UniversityBeijing 100875

We operated a p-type point contact high purity germanium(PPCGe)detector(CDEX-1B,1.008 kg)in the China Jinping Underground Laboratory(CJPL)for 500.3 days to search for neutrinoless double beta(0νββ)decay of ^(76)Ge.A total of 504.3 kg⋅day effective exposure data was *** anti-coincidence and the multi/single-site event(MSE/SSE)discrimination methods were used to suppress the background in the energy region of interest(ROI,1989–2089 keV for this work)with a factor of 23.A background level of 0.33 counts/(keV⋅kg⋅yr)was *** lower limit on the half life of^(76)Ge 0νββdecay was constrained as T_(1/2)^(0ν)>1.0×10^(23)yr(90%C.L.),corresponding to the upper limits on the effective Majorana neutrino mass:<3.2–7.5 eV.

关键词： neutrinoless double beta decay CDEX point-contact germanium detector ^(76)Ge

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钙钛矿太阳能电池中S形伏安特性研究

物理学报 2015年第3期64卷 54-59页

作者：石将建卫会云朱立峰许信徐余颛吕松涛吴会觉罗艳红李冬梅孟庆波中国科学院物理研究所中科院清洁能源重点实验室北京市新能源材料与器件重点实验室北京凝聚态国家实验室北京100190

本文从理论模拟和实验角度研究了钙钛矿太阳能电池的伏安特性,并着重探讨了由于界面电荷输运受限而产生的S形特征.理论模拟表明,当电池界面电荷输运速度逐渐降低时,出现界面电荷积累,影响电池输出性能.实验研究表明,当电池的背接触,光... 详细信息

本文从理论模拟和实验角度研究了钙钛矿太阳能电池的伏安特性,并着重探讨了由于界面电荷输运受限而产生的S形特征.理论模拟表明,当电池界面电荷输运速度逐渐降低时,出现界面电荷积累,影响电池输出性能.实验研究表明,当电池的背接触,光阳极等界面电荷输运速度受限时,即出现S形伏安特性,降低电池效率.然而,由于电子和空穴在界面输运性质方面的差异,以及电池中可能存在的独特的界面能带结构,不同界面电荷输运受限而产生的S形伏安曲线又各具特征,与电池内部实际的电荷分布以及输运方向有关.本文的研究结果有助于阐明钙钛矿太阳能电池中存在的影响电荷输运的界面因素,进而为界面设计和界面优化提供理论依据.

关键词：钙钛矿太阳能电池界面电荷输运 S形伏安特性

Expert consensus on the diagnosis and treatment of solid tumors with BRAF mutations

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The Innovation 2024年第6期5卷 100-116页

作者： Wenxian Wang Bin lian Chunwei Xu Qian Wang Ziming li Nan Zheng Aijun liu Jinpu Yu Wenzhao Zhong Zhijie Wang Yongchang Zhang Jingjing liu Shirong Zhang Xiuyu Cai Anwen liu Wen li lili Mao Ping Zhan Hongbing liu Tangfeng Lv liyun Miao lingfeng Min Yu Chen Jingping Yuan feng Wang Zhansheng jiang Gen lin Long Huang xingxiang Pu Rongbo lin weifeng liu Chuangzhou Rao Dongqing Lv Zongyang Yu Xiaoyan li Chuanhao Tang Chengzhi Zhou Junping Zhang Junli Xue Hui Guo Qian Chu Rui Meng Xuewen liu Jingxun Wu Rui Zhang Jin Zhou Zhengfei zhu Yongheng li Hong Qiu Fan Xia Yuanyuan Lu Xiaofeng Chen jian feng Rui Ge Enyong Dai Yu Han weiwei Pan Fei Pang xin Huang Meizhen Hu Qing Hao Kai Wang Fan Wu Binbin Song Bingwei Xu liping Wang Youcai zhu li lin Yanru Xie xinqing lin Jing Cai ling Xu Jisheng li Xiaodong Jiao Kainan li Jia wei Huijing feng lin Wang Yingying Du Wang Yao Xuefei Shi Xiaomin Niu Dongmei Yuan Yanwen Yao jianhui Huang yue feng Yinbin Zhang Pingli Sun Hong Wang Mingxiang Ye Dong Wang Zhaofeng Wang yue Hao Zhen Wang Bin Wan Donglai Lv Shengjie Yang Jin Kang Jiatao Zhang Chao Zhang Wenfeng li jianfei Fu lizhi Wu Shijie Lan Juanjuan Ou lin Shi Zhanqiang Zhai Yina Wang Bihui li Zhang Zhang Ke Wang Xuelei Ma Zhongwu li Zhefeng liu Nong Yang lin Wu Huijuan Wang Gu Jin Guansong Wang jiandong Wang Hubing Shi Meiyu Fang Yong Fang Yuan li Xiaojia Wang Jing Chen Yiping Zhang Xixu zhu Yi Shen Shenglin Ma Biyun Wang Yong Song Zhengbo Song Wenfeng Fang Yuanzhi Lu Lu Si Department of Chemotherapy Chinese Academy of Sciences University Cancer Hospital(Zhejiang Cancer Hospital)HangzhouZhejiang 310022P.R.China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Melanoma and SarcomaPeking University Cancer Hospital&InstituteBeijing 100142P.R.China Institute of Cancer and Basic Medicine(ICBM) Chinese Academy of SciencesHangzhouZhejiang 310022P.R.China Department of Respiratory Medicine Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjingJiangsu 210002P.R.China Department of Respiratory Medicine Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Chinese MedicineNanjingJiangsu 210029P.R.China Department of Shanghai Lung Cancer Center Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030P.R.China Beijing Key Laboratory of Mental Disorders National Clinical Research Center for Mental Disorders&National Center for Mental DisordersBeijing Anding HospitalCapital Medical UniversityBeijing 200030China Advanced Innovation Center for Human Brain Protection Capital Medical UniversityBeijing 200030China Senior Department of Pathology the 7th Medical Center of PLA General HospitalBeijing 100700P.R.China Department of Cancer Molecular Diagnostics Core Tianjin Medical University Cancer Institute and HospitalTianjin 300060P.R.China Guangdong Lung Cancer Institute Guangdong Provincial Laboratory of Translational Medicine in Lung CancerGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesSchool of MedicineGuangzhouGuangdong 510080P.R.China State Key Laboratory of Molecular Oncology Department of Medical OncologyNational Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China Department of Medical Oncology Lung Cancer and Gastrointestinal UnitHunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School

The BRAF gene is an important signaling molecule in human cells that is involved in the regulation of cell growth,differentiation,and *** the BRAF gene mutates,it can lead to abnormal activation of the signaling pathway,which promotes cell proliferation,inhibits cell apoptosis,and ultimately contributes to the occurrence and development of *** mutations are widely present in various cancers,including malignant melanoma,thyroid cancer,colorectal cancer,non-small cell lung cancer,and hairy cell leukemia,among *** is an important target for the treatment of various solid tumors,and targeted combination therapies,represented by BRAF inhibitors,have become one of the main treatment modalities for a variety of BRAF-mutation-positive solid tumors.

关键词： BRAF diagnosis treatment